FastQCFastQC Report
Fri 27 May 2016
SRR2139015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181892
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119250.049.9841231
GTATCAA220800.035.39411
TCAACGC270900.028.3593964
ATCAACG270950.028.3070053
CAACGCA276050.027.8457985
TATCAAC283900.027.3318542
AACGCAG286150.026.952256
ACGCAGA332150.023.155147
CGCAGAG332150.023.1415218
GCAGAGT383600.019.9261959
GTACATG266350.019.9083541
TACATGG260550.019.6956622
ACATGGG264050.019.256593
GAGTACT231900.019.0974512-13
AGAGTAC353600.018.6445110-11
CAGAGTA377600.017.83854110-11
GTGGTAT62350.017.6807041
AGTACTT253800.017.3148812-13
TGGTATC60250.016.6659262
CATGGGG192650.016.6122724
Produced by FastQC (version 0.11.5)