##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139015_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9181892
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506916766174115	34.0	31.0	34.0	31.0	34.0
2	32.64840928209567	34.0	31.0	34.0	31.0	34.0
3	32.642743565269555	34.0	31.0	34.0	31.0	34.0
4	36.201859812770614	37.0	35.0	37.0	35.0	37.0
5	36.13728412401279	37.0	35.0	37.0	35.0	37.0
6	36.16543039277744	37.0	36.0	37.0	35.0	37.0
7	36.11482034421664	37.0	36.0	37.0	35.0	37.0
8	36.102850806783614	37.0	36.0	37.0	35.0	37.0
9	37.810669086501996	39.0	38.0	39.0	35.0	39.0
10-11	37.75372515817001	39.0	38.0	39.0	35.0	39.0
12-13	37.72517717481321	39.0	38.0	39.0	35.0	39.0
14-15	39.07438254555815	40.0	38.0	41.0	36.0	41.0
16-17	39.05091417977907	40.0	38.0	41.0	36.0	41.0
18-19	38.975532820468814	40.0	38.5	41.0	35.0	41.0
20-21	39.03436491237803	40.0	39.0	41.0	35.0	41.0
22-23	38.96457207644495	40.0	38.0	41.0	35.0	41.0
24-25	38.890759378493385	40.0	38.0	41.0	35.0	41.0
26-27	38.81358225236798	40.0	38.0	41.0	35.0	41.0
28-29	38.60221827702165	40.0	38.0	41.0	34.5	41.0
30-31	38.52997182870026	40.0	38.0	41.0	34.0	41.0
32-33	38.464352056372434	40.0	38.0	41.0	34.0	41.0
34-35	38.27826733130101	40.0	38.0	41.0	34.0	41.0
36-37	38.40192314717716	40.0	38.0	41.0	34.0	41.0
38-39	38.5211431708534	40.0	38.0	41.0	34.0	41.0
40-41	38.520294154377254	40.0	38.0	41.0	34.0	41.0
42-43	38.39509127594786	40.0	38.0	41.0	34.0	41.0
44-45	38.33549947890476	40.0	38.0	41.0	34.0	41.0
46-47	38.35703024693689	40.0	38.0	41.0	34.0	41.0
48-49	38.234002426806825	40.0	38.0	41.0	33.0	41.0
50-51	38.174658321555754	40.0	37.5	41.0	33.0	41.0
52-53	38.104813570866085	40.0	37.0	41.0	33.0	41.0
54-55	37.99649341321809	40.0	37.0	41.0	33.0	41.0
56-57	37.84644018308238	40.0	37.0	41.0	33.0	41.0
58-59	37.69186146087958	39.5	36.0	41.0	33.0	41.0
60-61	37.511734113072634	39.0	36.0	41.0	33.0	41.0
62-63	37.297705570703	39.0	35.5	40.5	32.0	41.0
64-65	37.06097381709553	38.5	35.0	40.0	32.0	41.0
66-67	36.78884189404494	38.0	35.0	40.0	32.0	41.0
68-69	36.48234995905119	37.5	35.0	40.0	31.0	41.0
70-71	36.1874116955389	37.0	35.0	39.5	31.0	41.0
72-73	35.86001270798489	36.5	34.5	39.0	31.0	41.0
74-75	35.5382977350198	36.0	34.0	39.0	31.0	40.0
76-77	34.51548523203043	35.0	33.5	37.0	29.5	39.0
78-79	34.826366800390886	35.0	34.0	37.0	30.0	39.0
80-81	34.67242762222945	35.0	34.0	37.0	30.0	39.0
82-83	34.4050763277815	35.0	34.0	36.5	30.0	38.5
84-85	34.134679679807206	35.0	34.0	36.0	30.0	37.0
86-87	33.88372316977262	35.0	34.0	36.0	30.0	37.0
88-89	33.63684533672953	35.0	33.0	35.0	30.0	36.5
90-91	33.51076726282078	35.0	33.0	35.0	30.0	36.0
92-93	33.433311485922516	35.0	33.0	35.0	30.0	36.0
94-95	33.34386059038084	35.0	33.0	35.0	30.0	36.0
96-97	33.330757955430485	35.0	33.0	35.0	30.0	35.0
98-99	33.36176341149539	34.5	33.0	35.0	30.0	35.0
100-101	32.15512349033233	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	57.0
21	282.0
22	1276.0
23	4348.0
24	11185.0
25	22940.0
26	39722.0
27	61964.0
28	89146.0
29	118095.0
30	153083.0
31	191354.0
32	240021.0
33	310969.0
34	428003.0
35	615976.0
36	1026507.0
37	1984364.0
38	2621852.0
39	1209556.0
40	51182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.57679876870693	21.6307597606245	15.560877867001702	24.23156360366687
2	13.598765918832415	22.042744567241698	41.54314818775913	22.815341326166763
3	16.787922490807112	27.23139958964594	31.47401927742703	24.50665864211992
4	11.843483837072077	19.25477974217905	39.58382796718231	29.31790845356657
5	12.621850377728682	38.409918257258475	36.08303550861999	12.885195856392853
6	28.664669760662072	39.263903838335345	19.127822640941044	12.943603760061539
7	25.376984168042693	33.375704219484604	23.906252263353554	17.34105934911915
8	23.93469847046947	37.135063495463925	21.510085617943336	17.420152416123265
9	25.190952595661447	17.060911405750034	21.721252556671526	36.02688344191699
10-11	23.463640635882502	27.31764773791003	29.533227654234395	19.685483971973074
12-13	24.46372285355863	24.938864707531057	29.5191311113034	21.078281327606916
14-15	21.630735197171557	26.277159887896563	27.29730533310346	24.79479958182842
16-17	21.0162183226144	29.383928968662104	27.94651575373539	21.653336954988106
18-19	21.129975480536984	28.597899157902084	29.687810989230485	20.584314372330446
20-21	21.950928899662106	27.66519532073657	29.59687597477394	20.786999804827385
22-23	21.624950200301672	27.64175587949603	29.390331873631443	21.342962046570854
24-25	21.41881975336001	27.755107399521034	29.525919163581527	21.300153683537427
26-27	21.396094540513943	27.922730300143794	29.362826102291077	21.318349057051186
28-29	21.323534437436077	27.930823024689623	29.379600409110555	21.36604212876375
30-31	21.53726985085931	27.86799510999769	29.318185051783896	21.276549987359104
32-33	21.42996511919063	27.93841178526723	29.30782602172211	21.323797073820025
34-35	21.56229776755985	27.99938191476675	29.20312103379974	21.23519928387366
36-37	21.549279517310897	27.933764132587434	29.167688017373017	21.34926833272865
38-39	21.41804168364053	28.073999812322686	29.22527381887101	21.28268468516577
40-41	21.5875406318161	28.04802168932921	29.051414047403824	21.313023631450864
42-43	21.590073751787997	28.136109802990816	28.853491768048407	21.420324677172783
44-45	21.683225419022648	28.267830210287805	28.606819595528727	21.442124775160824
46-47	21.706259675822867	28.13048275182075	28.370672213075625	21.792585359280757
48-49	21.726931626106694	28.321242345073692	28.25162467692257	21.700201351897043
50-51	21.665108157906708	28.350909821831493	28.18899457345439	21.794987446807408
52-53	21.766450170654572	28.35401895848917	28.086881679789165	21.792649191067095
54-55	21.78167766682389	28.283215985309933	28.08290393267007	21.852202415196103
56-57	21.857139763460452	28.386064155626645	27.956319283944282	21.80047679696862
58-59	21.714571358665083	28.532926770179362	28.082428954021786	21.670072917133766
60-61	21.911367281488932	28.478555535601924	27.953386961979902	21.656690220929235
62-63	21.811756671205355	28.571877533562017	27.876861396567694	21.739504398664934
64-65	21.772874751517595	28.466234515471516	27.92856933593931	21.832321397071574
66-67	21.851035494446087	28.508030697534963	27.803504270335633	21.83742953768332
68-69	21.824736065932278	28.372826385923748	27.925301427970822	21.877136120173144
70-71	21.78795742054273	28.22085264495232	28.018485408691202	21.972704525813747
72-73	21.874050584229142	28.23674728131056	27.933487323587137	21.95571481087316
74-75	21.762184530227568	28.351691587052752	27.91683990981874	21.96928397290094
76-77	21.854045605212963	28.34914246257653	27.815381385607278	21.981430546603224
78-79	21.82354406704578	28.36061915544135	27.79406151007876	22.021775267434116
80-81	21.821570207860137	28.33899130458567	27.83789565435763	22.001542833196563
82-83	21.854835964539575	28.2905719091571	27.932741331706662	21.921850794596665
84-85	21.94792050944993	28.29325453985943	27.854735745790233	21.90408920490041
86-87	21.78788880412477	28.31833313751919	27.875166300333458	22.01861175802258
88-89	21.875147802868415	28.252388722756223	27.829836793305514	22.042626681069855
90-91	21.822696102070456	28.392308795463194	27.918869856262784	21.866125246203566
92-93	21.8963725244221	28.33014456542945	27.862176702195967	21.91130620795248
94-95	21.73290305663192	28.397402915054876	27.906154433197855	21.963539595115353
96-97	21.654233143608163	28.442691128164377	27.9991787195513	21.90389700867616
98-99	21.921751558157982	28.98917276967939	28.021673163212668	21.067402508949957
100-101	22.321388007195438	31.75592698262623	25.247529528569064	20.675155481609274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2992.0
1	2570.0
2	3150.0
3	4332.5
4	5700.5
5	8353.5
6	10322.0
7	12068.0
8	13370.0
9	13185.0
10	11975.0
11	10902.5
12	12378.0
13	17405.0
14	28962.0
15	44593.0
16	56658.5
17	62655.0
18	63035.5
19	59427.5
20	54150.5
21	49120.5
22	47080.5
23	51059.5
24	60686.0
25	75648.5
26	95040.0
27	115911.5
28	136944.0
29	159952.0
30	181084.5
31	201098.0
32	224941.0
33	248836.0
34	270815.5
35	289357.5
36	309131.0
37	324885.0
38	331811.5
39	337254.5
40	345675.5
41	354450.0
42	358462.5
43	363323.0
44	368388.0
45	369951.0
46	370289.0
47	368513.0
48	365989.5
49	359761.0
50	346944.5
51	332049.5
52	314462.0
53	294179.5
54	274552.5
55	253682.5
56	230908.0
57	207599.0
58	183570.5
59	158962.5
60	132763.0
61	106591.0
62	82685.0
63	62256.0
64	45957.5
65	34330.0
66	26259.0
67	20098.5
68	15047.5
69	10850.5
70	7704.5
71	5395.0
72	3738.5
73	2653.5
74	1903.0
75	1295.5
76	843.5
77	526.5
78	341.0
79	236.0
80	169.5
81	116.0
82	80.5
83	51.5
84	33.5
85	26.0
86	22.0
87	18.5
88	13.0
89	8.0
90	6.5
91	5.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.079151007221606E-4
4	1.742560247931472E-4
5	4.029670573341529E-4
6	2.2871103254100574E-4
7	0.0029732434230330743
8	5.990050852264435E-4
9	3.485120495862944E-4
10-11	7.62370108470019E-5
12-13	2.7227503873929253E-5
14-15	1.633650232435755E-4
16-17	9.311806324883804E-4
18-19	2.7227503873929253E-5
20-21	0.0
22-23	0.002968150923666783
24-25	0.007473611532893832
26-27	0.03647179495451161
28-29	0.28134595459751643
30-31	0.24587503443134806
32-33	0.25275348797570696
34-35	0.13403337377795277
36-37	0.0256683620087311
38-39	0.01663226765156867
40-41	0.009570332215382529
42-43	0.006241912387237917
44-45	0.007043733260163062
46-47	0.001687137072509491
48-49	0.002165399821155298
50-51	0.00284768916139223
52-53	0.001989536563573851
54-55	2.1294016062076314E-4
56-57	0.0017718048929018513
58-59	0.003208022246460613
60-61	0.013753766649648027
62-63	0.00866780599820751
64-65	0.002435164065779591
66-67	0.0017100772480588128
68-69	0.0023072638781764672
70-71	0.004158101592928604
72-73	0.0035885181310843233
74-75	0.0037991900126892945
76-77	0.0015941724926126833
78-79	8.618452172396888E-5
80-81	3.5530489651711366E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	4.7731935172337935E-4
92-93	0.00792159757807273
94-95	0.0010797199074349512
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	159.0
20-21	662.0
22-23	1332.0
24-25	3170.0
26-27	4806.0
28-29	8102.0
30-31	17683.0
32-33	23647.0
34-35	34278.0
36-37	46531.0
38-39	56596.0
40-41	60612.0
42-43	72554.0
44-45	87080.0
46-47	86430.0
48-49	75150.0
50-51	67365.0
52-53	65358.0
54-55	70483.0
56-57	74252.0
58-59	73326.0
60-61	72432.0
62-63	71386.0
64-65	76897.0
66-67	80236.0
68-69	79413.0
70-71	78341.0
72-73	75525.0
74-75	77333.0
76-77	78408.0
78-79	82581.0
80-81	85361.0
82-83	83159.0
84-85	84608.0
86-87	88661.0
88-89	95043.0
90-91	99382.0
92-93	107344.0
94-95	121390.0
96-97	185613.0
98-99	781395.0
100-101	5747808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.74508952266045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13972243068085	74.65014034982471
2	7.301815447800148	12.229823767079367
3	1.9502998139851024	4.899840975546313
4	0.7282179329805099	2.439387039578382
5	0.3428046040990615	1.435410112952804
6	0.18168991025026357	0.912938267956348
7	0.10228898804295702	0.5996340322587848
8	0.06617281420898594	0.44333185999183244
9	0.04335569303191895	0.32677437548475347
>10	0.14069098526651092	1.7640506309149373
>50	0.002161439850019411	0.11368998202990978
>100	6.68670799465351E-4	0.0957290762912389
>500	6.676195425209498E-5	0.035987172007043135
>1k	4.45070500618279E-5	0.05326235808357407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.26730046487151E-5	0.0	0.0	0.0
3	0.0	3.26730046487151E-5	0.0	0.0	0.0
4	0.0	7.623701084700191E-5	0.0	1.08910015495717E-5	0.0
5	0.0	8.71280123965736E-5	0.0	1.08910015495717E-5	0.0
6	5.44550077478585E-5	8.71280123965736E-5	0.0	4.35640061982868E-5	1.08910015495717E-5
7	5.44550077478585E-5	8.71280123965736E-5	0.0	4.35640061982868E-5	2.17820030991434E-5
8	5.44550077478585E-5	9.80190139461453E-5	0.0	5.44550077478585E-5	2.17820030991434E-5
9	5.44550077478585E-5	1.742560247931472E-4	0.0	8.71280123965736E-5	3.26730046487151E-5
10-11	6.534600929743021E-5	1.742560247931472E-4	0.0	9.80190139461453E-5	3.26730046487151E-5
12-13	7.623701084700191E-5	1.742560247931472E-4	0.0	1.08910015495717E-4	3.26730046487151E-5
14-15	8.71280123965736E-5	2.0148352866707645E-4	0.0	1.5247402169400382E-4	3.811850542350095E-5
16-17	1.306920185948604E-4	2.0692902944186232E-4	0.0	1.5247402169400382E-4	6.53460092974302E-5
18-19	1.306920185948604E-4	2.2326553176621987E-4	0.0	1.742560247931472E-4	8.71280123965736E-5
20-21	1.306920185948604E-4	2.4504753486536324E-4	0.0	1.8514702634271892E-4	9.80190139461453E-5
22-23	1.3613751936964624E-4	3.049480433880076E-4	0.0	1.9059252711750476E-4	1.0346451472093116E-4
24-25	1.415830201444321E-4	3.9207605578458126E-4	0.0	2.3415653331579155E-4	1.1980101704528871E-4
26-27	1.415830201444321E-4	4.1385805888372463E-4	0.0	2.831660402888642E-4	1.415830201444321E-4
28-29	1.415830201444321E-4	4.247490604332963E-4	0.0	4.1930355965851045E-4	1.415830201444321E-4
30-31	1.415830201444321E-4	4.955405705055123E-4	0.0	9.747446386866672E-4	1.415830201444321E-4
32-33	1.415830201444321E-4	5.009860712802982E-4	0.0	0.0021999823130134837	1.5247402169400382E-4
34-35	1.5247402169400382E-4	5.826685829020859E-4	0.0	0.003986106567143243	1.5247402169400382E-4
36-37	1.5247402169400382E-4	6.589055937490879E-4	0.0	0.006289553394877656	1.5247402169400382E-4
38-39	1.5247402169400382E-4	6.643510945238737E-4	0.0	0.01034100597131833	1.633650232435755E-4
40-41	1.5247402169400382E-4	7.514791069204473E-4	0.0	0.014860771614390585	1.633650232435755E-4
42-43	1.5247402169400382E-4	7.732611100195908E-4	0.0	0.019048361710200905	1.633650232435755E-4
44-45	1.5791952246878966E-4	8.658346231909502E-4	0.0	0.023954757908282956	1.633650232435755E-4
46-47	1.633650232435755E-4	9.366261332631662E-4	0.0	0.02979233473885339	1.633650232435755E-4
48-49	1.633650232435755E-4	9.420716340379521E-4	0.0	0.035422982539981954	1.960380278922906E-4
50-51	1.6881052401836134E-4	0.0010400906479840974	0.0	0.04124966836900282	1.960380278922906E-4
52-53	1.742560247931472E-4	0.0010455361487588832	0.0	0.04662982313449124	2.0692902944186232E-4
54-55	1.742560247931472E-4	0.0010618726510832409	0.0	0.0522223524301963	2.17820030991434E-4
56-57	1.742560247931472E-4	0.00108910015495717	0.0	0.057891118736748376	2.287110325410057E-4
58-59	1.742560247931472E-4	0.00108910015495717	0.0	0.06383760558281452	2.3960203409057742E-4
60-61	1.960380278922906E-4	0.0010999911565067418	0.0	0.06945736238239353	2.613840371897208E-4
62-63	2.504930356401491E-4	0.0011108821580563134	0.0	0.07482662614633237	2.6682953796450666E-4
64-65	2.504930356401491E-4	0.0012415741766511738	0.0	0.08031024542654172	2.722750387392925E-4
66-67	2.613840371897208E-4	0.0012415741766511738	0.0	0.08612059475323822	2.722750387392925E-4
68-69	2.613840371897208E-4	0.0012579106789755313	0.0	0.09175124255436679	2.831660402888642E-4
70-71	2.7772053951407834E-4	0.001274247181299889	0.0	0.09734377185007186	2.940570418384359E-4
72-73	2.831660402888642E-4	0.0012796926820746748	0.0	0.10201056601406333	3.158390449375793E-4
74-75	2.831660402888642E-4	0.0013014746851738182	0.0	0.10571350654091771	3.26730046487151E-4
76-77	2.831660402888642E-4	0.0013613751936964627	0.0	0.10916050853135716	3.3217554726193687E-4
78-79	2.831660402888642E-4	0.0013940481983451777	0.0	0.11250404600707567	3.4306654881150855E-4
80-81	2.831660402888642E-4	0.0013940481983451777	0.0	0.11362037366590677	3.539575503610803E-4
82-83	2.831660402888642E-4	0.0013940481983451777	0.0	0.11420848774958364	3.811850542350095E-4
84-85	2.831660402888642E-4	0.0013994936991199637	0.0	0.11458967280381865	3.811850542350095E-4
86-87	2.9405704183843595E-4	0.0014376122045434644	0.0	0.1147203648224135	3.866305550097954E-4
88-89	3.485120495862944E-4	0.0015138492153904664	0.0	0.11474759232628744	4.029670573341529E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11925	0.0	49.984123	1
GTATCAA	22080	0.0	35.3941	1
TCAACGC	27090	0.0	28.359396	4
ATCAACG	27095	0.0	28.307005	3
CAACGCA	27605	0.0	27.845798	5
TATCAAC	28390	0.0	27.331854	2
AACGCAG	28615	0.0	26.95225	6
ACGCAGA	33215	0.0	23.15514	7
CGCAGAG	33215	0.0	23.141521	8
GCAGAGT	38360	0.0	19.926195	9
GTACATG	26635	0.0	19.908354	1
TACATGG	26055	0.0	19.695662	2
ACATGGG	26405	0.0	19.25659	3
GAGTACT	23190	0.0	19.09745	12-13
AGAGTAC	35360	0.0	18.64451	10-11
CAGAGTA	37760	0.0	17.838541	10-11
GTGGTAT	6235	0.0	17.680704	1
AGTACTT	25380	0.0	17.31488	12-13
TGGTATC	6025	0.0	16.665926	2
CATGGGG	19265	0.0	16.612272	4
>>END_MODULE