FastQCFastQC Report
Fri 27 May 2016
SRR2139014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12773145
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179450.049.1507721
GTATCAA331400.036.5566561
ATCAACG418050.028.3991953
TCAACGC422600.028.1536464
CAACGCA427900.027.8049355
TATCAAC434000.027.5711692
AACGCAG437650.027.2826526
ACGCAGA500550.023.8115587
CGCAGAG504800.023.6106228
TACATGG402450.020.4881042
GCAGAGT587000.020.1884659
GTACATG419550.020.1299171
ACATGGG406300.019.770193
AGAGTAC537150.019.14991410-11
GAGTACT351750.019.08438912-13
CATGGGG297950.017.9015254
CAGAGTA572000.017.8827110-11
AGTACTT379300.017.78812-13
GTACTTT386600.017.25979814-15
GTGGTAT96150.015.7610371
Produced by FastQC (version 0.11.5)