FastQCFastQC Report
Fri 27 May 2016
SRR2139013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10993726
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157750.048.864091
GTATCAA279650.035.7190251
ATCAACG336200.028.9852143
TCAACGC353900.027.606884
CAACGCA361250.027.0218935
AACGCAG362700.026.983476
TATCAAC366750.026.9033552
ACGCAGA366300.026.6722097
CGCAGAG431600.022.636058
GCAGAGT493000.019.6890379
GTACATG352750.019.5738051
TACATGG346750.019.2451112
ACATGGG340300.019.0906623
GAGTACT290650.018.90595612-13
AGAGTAC457500.018.61879310-11
CAGAGTA492050.017.62783210-11
GTGGTAT77150.017.5611781
AGTACTT317900.017.18609412-13
GTACTTT324700.016.936914-15
TGGTATC75850.016.36442
Produced by FastQC (version 0.11.5)