##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10993726
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6292307994578	34.0	31.0	34.0	31.0	34.0
2	32.75831615232179	34.0	31.0	34.0	31.0	34.0
3	33.41243014424773	34.0	31.0	34.0	31.0	37.0
4	36.18996625893714	37.0	35.0	37.0	35.0	37.0
5	36.149288785258065	37.0	35.0	37.0	35.0	37.0
6	36.17742465111464	37.0	36.0	37.0	35.0	37.0
7	36.18658351135912	37.0	36.0	37.0	35.0	37.0
8	36.46700208828199	37.0	37.0	37.0	35.0	39.0
9	37.809550101576114	39.0	38.0	39.0	35.0	39.0
10-11	37.77373708422422	39.0	38.0	39.0	35.0	39.0
12-13	37.91813885483411	39.0	38.0	39.0	35.0	40.0
14-15	39.08074610009382	40.0	38.0	41.0	36.0	41.0
16-17	39.03986346394298	40.0	38.0	41.0	36.0	41.0
18-19	38.998138119869466	40.0	38.5	41.0	35.0	41.0
20-21	39.042943102150446	40.0	39.0	41.0	35.0	41.0
22-23	38.979828991990644	40.0	38.0	41.0	35.0	41.0
24-25	38.899828725892135	40.0	38.0	41.0	35.0	41.0
26-27	38.82093983935745	40.0	38.0	41.0	35.0	41.0
28-29	38.60038700418805	40.0	38.0	41.0	34.5	41.0
30-31	38.54303168023211	40.0	38.0	41.0	34.0	41.0
32-33	38.45562878353998	40.0	38.0	41.0	34.0	41.0
34-35	38.296834593445226	40.0	38.0	41.0	34.0	41.0
36-37	38.43314596699102	40.0	38.0	41.0	34.0	41.0
38-39	38.530933488850366	40.0	38.0	41.0	34.0	41.0
40-41	38.51629925995364	40.0	38.0	41.0	34.0	41.0
42-43	38.40653819592414	40.0	38.0	41.0	34.0	41.0
44-45	38.35896075273719	40.0	38.0	41.0	34.0	41.0
46-47	38.3580777660449	40.0	38.0	41.0	34.0	41.0
48-49	38.256540040190416	40.0	38.0	41.0	33.0	41.0
50-51	38.187016592155594	40.0	38.0	41.0	33.0	41.0
52-53	38.12344627981631	40.0	37.0	41.0	33.0	41.0
54-55	38.021268774810494	40.0	37.0	41.0	33.0	41.0
56-57	37.8702207591008	40.0	37.0	41.0	33.0	41.0
58-59	37.72143645802574	39.5	36.0	41.0	33.0	41.0
60-61	37.54300382675443	39.0	36.0	41.0	33.0	41.0
62-63	37.33717846886389	39.0	35.5	41.0	32.0	41.0
64-65	37.1025454443261	39.0	35.0	40.0	32.0	41.0
66-67	36.83226802416233	38.0	35.0	40.0	32.0	41.0
68-69	36.53236231660118	37.5	35.0	40.0	31.0	41.0
70-71	36.233676380955984	37.0	35.0	39.5	31.0	41.0
72-73	35.904523050276836	36.5	35.0	39.0	31.0	41.0
74-75	35.4781919413325	36.0	34.0	39.0	30.5	40.0
76-77	34.6579470788657	35.0	33.5	37.0	29.5	39.0
78-79	34.884189789203475	35.0	34.0	37.0	30.0	39.0
80-81	34.70383300153986	35.0	34.0	37.0	30.5	39.0
82-83	34.43070592162756	35.0	34.0	36.5	30.0	38.5
84-85	34.15645949740053	35.0	34.0	36.0	30.0	37.0
86-87	33.89827291725865	35.0	34.0	36.0	30.0	37.0
88-89	33.66561051506717	35.0	33.5	35.0	30.0	36.5
90-91	33.542937006730426	35.0	33.0	35.0	30.0	36.0
92-93	33.462191138330226	35.0	33.0	35.0	30.0	36.0
94-95	33.38028638924679	35.0	33.0	35.0	30.0	36.0
96-97	33.370366833249705	35.0	33.0	35.0	30.0	35.0
98-99	33.36774876501343	34.5	33.0	35.0	30.0	35.0
100-101	31.96588646778207	33.0	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	15.0
20	63.0
21	331.0
22	1420.0
23	4783.0
24	12096.0
25	25121.0
26	45040.0
27	71053.0
28	101661.0
29	137718.0
30	178407.0
31	224646.0
32	284568.0
33	370139.0
34	509079.0
35	723455.0
36	1175574.0
37	2260075.0
38	3137633.0
39	1626254.0
40	104590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65562521148438	0.020893746611701954	27.15027306498619	30.17320797691773
2	14.021595629353742	25.569093806947357	38.21368335664061	22.195627207058294
3	15.26333096075698	25.701232754288263	34.300031609084314	24.73540467587044
4	12.373264857720722	24.878177901060564	38.04331482727145	24.705242413947268
5	16.648697256415343	37.23852143089809	32.730196058954945	13.382585253731621
6	26.58210634483485	38.566732744199356	21.076492240839737	13.774668670126058
7	25.073566184824454	33.593353601063605	23.371178719938378	17.961901494173556
8	23.541334657730623	32.641162922697404	22.895590921329344	20.921911498242626
9	23.34042619573917	20.961495972095243	24.515674049483515	31.182403782682073
10-11	23.925711385876134	26.71005175046802	29.419390184411697	19.944846679244154
12-13	24.308878159531076	24.920450038749394	29.233971758422083	21.536700043297447
14-15	21.793975069456803	27.340563623532326	27.320274737262107	23.545186569748765
16-17	20.758959575586918	29.584384737742198	28.326533485894423	21.33012220077647
18-19	20.93035842280866	28.637913281400657	30.031304235508998	20.40042406028169
20-21	21.752305242286834	27.892242311303246	29.86007493828294	20.495377508126985
22-23	21.498393516241194	27.76255208438049	29.673047326428385	21.066007072949926
24-25	21.232084483276243	28.02543165460097	29.818701017937787	20.923782844185
26-27	21.175945321190362	28.17311701108754	29.66595896283423	20.984978704887865
28-29	21.148118355544927	28.05677133126877	29.650645831304672	21.144464481881627
30-31	21.43862647756407	28.01536470014783	29.620935169854423	20.925073652433674
32-33	21.172246084378713	28.29589734084088	29.4998957692204	21.03196080556
34-35	21.380186136983625	28.16941571023183	29.517080758819887	20.93331739396466
36-37	21.32239326511907	28.242215592995745	29.477208759949143	20.95818238193604
38-39	21.16649317391492	28.36735044313746	29.483554697090007	20.98260168585761
40-41	21.43258291335052	28.09538836037192	29.31169124552192	21.16033748075564
42-43	21.379896404850093	28.13707667418693	29.20272384140487	21.280303079558102
44-45	21.465344207716385	28.487523210667465	28.84251856496584	21.204614016650307
46-47	21.57209382110136	28.29581659474644	28.59575103459632	21.53633854955588
48-49	21.42736617175162	28.514660315466717	28.60600912924226	21.4519643835394
50-51	21.483496520050853	28.66823169667665	28.34620120518892	21.502070578083572
52-53	21.654577026450976	28.420567699980293	28.36703893243846	21.557816341130277
54-55	21.575788023630206	28.43782565372249	28.40991399382637	21.57647232882093
56-57	21.595122383468926	28.717560925447177	28.168756946227468	21.51855974485643
58-59	21.594845610237474	28.568867383968932	28.325347934042146	21.510939071751448
60-61	21.641795959155694	28.6511353106014	28.239092994488335	21.46797573575457
62-63	21.58965410174857	28.79845684605703	28.156293487712464	21.45559556448194
64-65	21.641473636146543	28.571216387497344	28.140021150914134	21.64728882544198
66-67	21.628644472398744	28.639585172676153	28.0991885082159	21.632581846709208
68-69	21.680871422293862	28.619244508349993	28.135989935466043	21.5638941338901
70-71	21.658031746279917	28.29980339351394	28.23983776381945	21.80232709638669
72-73	21.638120850436273	28.432929633368527	28.208439199972375	21.720510316222825
74-75	21.541505311869326	28.56801330257529	28.19019462511555	21.70028676043983
76-77	21.73129460073915	28.318899353553096	28.083827751131672	21.86597829457608
78-79	21.54601294796731	28.43135460188605	28.175274198870394	21.84735825127625
80-81	21.630028036545333	28.48400267876745	28.11153516164413	21.774434123043083
82-83	21.72331656450241	28.309138766912046	28.212784723428026	21.754759945157517
84-85	21.779789606805686	28.356073255161203	28.171314652290754	21.69282248574236
86-87	21.63672932271386	28.3748776417523	28.179655446840577	21.80873758869326
88-89	21.727712248279072	28.226389252210232	28.206768380439527	21.839130119071164
90-91	21.78721003862243	28.264533401797937	28.216684297124857	21.731572262454776
92-93	21.71473010204152	28.240633871739707	28.288941449608927	21.755694576609844
94-95	21.693113205075026	28.12857958216877	28.306605573426612	21.871701639329594
96-97	21.60293367644635	28.30328708189557	28.40363338746285	21.69014585419523
98-99	21.963033961060656	29.196916700729542	28.323720007526447	20.516329330683355
100-101	22.279386488950873	31.36025991232704	25.298662596065768	21.061691002656318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2628.0
1	2289.0
2	3122.5
3	4504.5
4	6066.0
5	9165.5
6	11501.0
7	13688.5
8	15728.5
9	16076.5
10	14921.5
11	13734.0
12	15242.5
13	20153.5
14	32887.0
15	52350.5
16	69217.5
17	78478.5
18	79444.5
19	74869.5
20	68242.5
21	62000.0
22	59296.0
23	62922.5
24	72650.5
25	88940.5
26	110726.5
27	135640.0
28	164362.5
29	194982.0
30	225691.0
31	255848.5
32	286656.5
33	317429.5
34	346085.5
35	370096.5
36	393592.5
37	415326.5
38	428811.0
39	438033.5
40	444694.0
41	449483.0
42	451610.5
43	452970.0
44	454977.5
45	454922.5
46	451906.5
47	446369.5
48	436479.5
49	422854.5
50	409755.0
51	392248.0
52	367805.0
53	342582.0
54	318345.0
55	293605.0
56	267847.0
57	240179.0
58	210373.0
59	179852.5
60	149028.0
61	119794.0
62	93987.5
63	72231.0
64	54330.0
65	40769.0
66	30955.0
67	23509.5
68	17723.5
69	12437.5
70	8561.5
71	6057.5
72	4183.5
73	2866.5
74	1916.5
75	1306.5
76	870.5
77	568.0
78	379.0
79	264.0
80	183.5
81	120.5
82	85.0
83	64.0
84	50.0
85	40.0
86	31.0
87	21.5
88	12.0
89	6.0
90	5.0
91	5.5
92	5.0
93	4.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.457658304381972E-5
2	1.8192194347939908E-4
3	4.6390095587246766E-4
4	2.819790123930685E-4
5	3.9113217848070795E-4
6	6.822072880477465E-4
7	0.0020375257669692694
8	4.1842047000261785E-4
9	4.0932437282864786E-4
10-11	8.186487456572957E-5
12-13	5.457658304381972E-5
14-15	2.0466218641432393E-4
16-17	8.823214258750855E-4
18-19	5.457658304381972E-5
20-21	2.228622563438992E-4
22-23	0.003102154587367954
24-25	0.010937423650731849
26-27	0.07356307692475751
28-29	0.2750449351804727
30-31	0.24906705899996606
32-33	0.2478011120484689
34-35	0.10778214720442865
36-37	0.02217089737087458
38-39	0.013224849352724829
40-41	0.00660891422877266
42-43	0.004061022755860923
44-45	0.005318605395063916
46-47	9.283873728542226E-4
48-49	0.001599344317442138
50-51	0.0020266659181579303
52-53	9.009662367853128E-4
54-55	1.0988830403336529E-4
56-57	0.0011095180561203311
58-59	0.0026229655146245605
60-61	0.010597043610023857
62-63	0.006203238672465361
64-65	0.0012543436898561225
66-67	0.001846605064346227
68-69	0.0026889990633051563
70-71	0.004299360567828275
72-73	0.003924983734647824
74-75	0.0036641811304665
76-77	0.001319915354670804
78-79	9.664689290188266E-5
80-81	3.0516344611752064E-4
82-83	2.3330271429627125E-5
84-85	0.0
86-87	1.1978290067949247E-5
88-89	5.4688061122293806E-5
90-91	0.0013886107381576962
92-93	0.007486894326408887
94-95	8.308747961161076E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	223.0
20-21	802.0
22-23	1712.0
24-25	4410.0
26-27	6665.0
28-29	12218.0
30-31	24652.0
32-33	33235.0
34-35	48952.0
36-37	66294.0
38-39	77024.0
40-41	81800.0
42-43	97615.0
44-45	114965.0
46-47	112331.0
48-49	99100.0
50-51	89539.0
52-53	88927.0
54-55	94423.0
56-57	97418.0
58-59	97278.0
60-61	96110.0
62-63	96065.0
64-65	102045.0
66-67	107915.0
68-69	105334.0
70-71	103037.0
72-73	101741.0
74-75	103824.0
76-77	105600.0
78-79	110395.0
80-81	111860.0
82-83	109971.0
84-85	112958.0
86-87	118139.0
88-89	124887.0
90-91	133098.0
92-93	140014.0
94-95	159343.0
96-97	278850.0
98-99	1075407.0
100-101	6347550.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.51512412297188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71222766580944	74.08812704735416
2	7.587888603773693	12.674069171508876
3	2.039125356151546	5.108934216638867
4	0.7941356374215289	2.652893453189375
5	0.3436387128169434	1.4349514877182659
6	0.19154762783184642	0.9598274348302486
7	0.10228627356147382	0.5979715582793919
8	0.05959845219171637	0.3981897705862558
9	0.043264804516758466	0.32519389674358784
>10	0.12337886790896738	1.5471213565531134
>50	0.0025032805409145264	0.1386576912740621
>100	3.6820434372525444E-4	0.05270235748555668
>500	3.651313141675631E-5	0.021360557838259117
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.096097173969953E-6
2	0.0	3.6384388695879813E-5	0.0	9.096097173969953E-6	9.096097173969953E-6
3	0.0	4.548048586984977E-5	0.0	3.6384388695879813E-5	9.096097173969953E-6
4	0.0	4.548048586984977E-5	0.0	3.6384388695879813E-5	1.8192194347939907E-5
5	0.0	4.548048586984977E-5	0.0	3.6384388695879813E-5	1.8192194347939907E-5
6	0.0	4.548048586984977E-5	0.0	3.6384388695879813E-5	1.8192194347939907E-5
7	0.0	5.457658304381972E-5	0.0	3.6384388695879813E-5	2.728829152190986E-5
8	0.0	8.186487456572958E-5	0.0	5.457658304381972E-5	4.548048586984977E-5
9	0.0	1.182492632616094E-4	0.0	5.457658304381972E-5	6.367268021778967E-5
10-11	9.096097173969953E-6	1.2279731184859438E-4	0.0	6.367268021778967E-5	6.367268021778967E-5
12-13	9.096097173969953E-6	1.364414576095493E-4	0.0	8.186487456572958E-5	6.367268021778967E-5
14-15	9.096097173969953E-6	2.2285438076226387E-4	0.0	1.0915316608763944E-4	1.000570689136695E-4
16-17	9.096097173969953E-6	2.728829152190986E-4	0.0	1.364414576095493E-4	1.1824926326160938E-4
18-19	9.096097173969953E-6	2.728829152190986E-4	0.0	1.5008560337050422E-4	1.2734536043557934E-4
20-21	9.096097173969953E-6	2.728829152190986E-4	0.0	1.7282584630542912E-4	1.2734536043557934E-4
22-23	9.096097173969953E-6	3.0017120674100844E-4	0.0	1.91018040653369E-4	1.4553755478351925E-4
24-25	9.096097173969953E-6	3.4110364402387323E-4	0.0	2.1830633217527888E-4	1.7282584630542912E-4
26-27	9.096097173969953E-6	3.6384388695879816E-4	0.0	2.8652706098005354E-4	1.7282584630542912E-4
28-29	1.8192194347939907E-5	3.82036081306738E-4	0.0	7.140436281566414E-4	1.7282584630542912E-4
30-31	1.8192194347939907E-5	4.093243728286479E-4	0.0	0.0020102374754473597	1.7737389489241412E-4
32-33	1.8192194347939907E-5	4.1842047000261785E-4	0.0	0.004375222740679548	2.1830633217527888E-4
34-35	1.8192194347939907E-5	4.911892473943775E-4	0.0	0.007376934808089632	2.546907208711587E-4
36-37	1.8192194347939907E-5	5.594099761991522E-4	0.0	0.012193318261706722	2.546907208711587E-4
38-39	1.8192194347939907E-5	6.048904620690018E-4	0.0	0.01889714187892258	2.819790123930686E-4
40-41	1.8192194347939907E-5	6.458228993518667E-4	0.0	0.026369585707338897	3.0017120674100844E-4
42-43	1.8192194347939907E-5	7.185916767436264E-4	0.0	0.03347363760020943	3.0926730391497837E-4
44-45	1.8192194347939907E-5	7.731682597874461E-4	0.0	0.04191936382624053	3.2291144967593337E-4
46-47	1.8192194347939907E-5	7.731682597874461E-4	0.0	0.05066526125901264	3.4110364402387323E-4
48-49	1.8192194347939907E-5	7.82264356961416E-4	0.0	0.0595157638092854	3.6384388695879816E-4
50-51	1.8192194347939907E-5	8.914175230490555E-4	0.0	0.06842084294260199	3.6384388695879816E-4
52-53	1.8192194347939907E-5	9.096097173969953E-4	0.0	0.07680289648841529	4.0477632424166295E-4
54-55	1.8192194347939907E-5	9.232538631579502E-4	0.0	0.08570797562173188	4.457087615245277E-4
56-57	1.8192194347939907E-5	9.323499603319202E-4	0.0	0.09298940140949483	4.502568101115127E-4
58-59	1.8192194347939907E-5	9.459941060928752E-4	0.0	0.09894734505844516	4.548048586984977E-4
60-61	1.8192194347939907E-5	9.687343490278E-4	0.0	0.10454599286902366	4.548048586984977E-4
62-63	1.8192194347939907E-5	9.9602264054971E-4	0.0	0.11011280433949328	4.820931502204075E-4
64-65	1.8192194347939907E-5	0.0010642433693544846	0.0	0.11596159482235596	4.820931502204075E-4
66-67	1.8192194347939907E-5	0.0011188199523983042	0.0	0.12187405798543642	4.957372959813625E-4
68-69	1.8192194347939907E-5	0.0011370121467462442	0.0	0.12804121186938805	5.093814417423174E-4
70-71	1.8192194347939907E-5	0.0011506562925071991	0.0	0.13387181015790278	5.503138790251822E-4
72-73	1.8192194347939907E-5	0.001164300438268154	0.0	0.13854720410532334	6.640150936998066E-4
74-75	1.8192194347939907E-5	0.0012234250698989588	0.0	0.14235846882121675	6.640150936998066E-4
76-77	1.8192194347939907E-5	0.0012961938472907182	0.0	0.14566944819254182	6.685631422867916E-4
78-79	1.8192194347939907E-5	0.0013189340902256433	0.0	0.14859384343397317	6.731111908737765E-4
80-81	1.8192194347939907E-5	0.0013189340902256433	0.0	0.14981272045528513	7.185916767436263E-4
82-83	1.8192194347939907E-5	0.0013325782359865982	0.0	0.15039032262583224	7.73168259787446E-4
84-85	1.8192194347939907E-5	0.001355318478921523	0.0	0.15066320554105134	7.82264356961416E-4
86-87	1.8192194347939907E-5	0.001373510673269463	0.0	0.15076326260996498	8.00456551309356E-4
88-89	1.8192194347939907E-5	0.0014280872563132826	0.0	0.15083148333876978	8.186487456572958E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15775	0.0	48.86409	1
GTATCAA	27965	0.0	35.719025	1
ATCAACG	33620	0.0	28.985214	3
TCAACGC	35390	0.0	27.60688	4
CAACGCA	36125	0.0	27.021893	5
AACGCAG	36270	0.0	26.98347	6
TATCAAC	36675	0.0	26.903355	2
ACGCAGA	36630	0.0	26.672209	7
CGCAGAG	43160	0.0	22.63605	8
GCAGAGT	49300	0.0	19.689037	9
GTACATG	35275	0.0	19.573805	1
TACATGG	34675	0.0	19.245111	2
ACATGGG	34030	0.0	19.090662	3
GAGTACT	29065	0.0	18.905956	12-13
AGAGTAC	45750	0.0	18.618793	10-11
CAGAGTA	49205	0.0	17.627832	10-11
GTGGTAT	7715	0.0	17.561178	1
AGTACTT	31790	0.0	17.186094	12-13
GTACTTT	32470	0.0	16.9369	14-15
TGGTATC	7585	0.0	16.3644	2
>>END_MODULE