FastQCFastQC Report
Fri 27 May 2016
SRR2139012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9445648
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125850.049.0526121
GTATCAA238550.038.0742531
ATCAACG298150.029.8329143
TCAACGC300050.029.8294184
CAACGCA306850.029.126615
TATCAAC309050.029.0019052
AACGCAG316500.028.2925426
ACGCAGA358200.024.9630747
CGCAGAG367650.024.3324038
GCAGAGT418900.021.1615929
GTACATG287900.020.9230481
GTGGTAT63300.020.5171661
TACATGG290600.020.4347342
ACATGGG297050.019.473273
AGAGTAC386750.019.34183110-11
GAGTACT254150.019.08710112-13
TGGTATC63500.018.9052492
TATACCG8400.018.3095475
CAGAGTA409900.018.2077710-11
CATGGGG212500.018.1142464
Produced by FastQC (version 0.11.5)