##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9445648
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51854557781531	34.0	31.0	34.0	31.0	34.0
2	32.66431662496845	34.0	31.0	34.0	31.0	34.0
3	32.65544799043962	34.0	31.0	34.0	31.0	34.0
4	36.20778532081653	37.0	35.0	37.0	35.0	37.0
5	36.14826616448125	37.0	35.0	37.0	35.0	37.0
6	36.174506079413504	37.0	36.0	37.0	35.0	37.0
7	36.126418431006535	37.0	36.0	37.0	35.0	37.0
8	36.115165523847594	37.0	36.0	37.0	35.0	37.0
9	37.828615358099306	39.0	38.0	39.0	35.0	39.0
10-11	37.77155161826907	39.0	38.0	39.0	35.0	39.0
12-13	37.74115597998147	39.0	38.0	39.0	35.0	39.0
14-15	39.09269231713907	40.0	38.0	41.0	36.0	41.0
16-17	39.069540808634834	40.0	38.0	41.0	36.0	41.0
18-19	38.995251040479175	40.0	38.5	41.0	35.0	41.0
20-21	39.05793782669902	40.0	39.0	41.0	35.5	41.0
22-23	38.99016331776642	40.0	38.5	41.0	35.0	41.0
24-25	38.91924127502942	40.0	38.0	41.0	35.0	41.0
26-27	38.84213303437526	40.0	38.0	41.0	35.0	41.0
28-29	38.62664298826009	40.0	38.0	41.0	34.5	41.0
30-31	38.553193516726864	40.0	38.0	41.0	34.0	41.0
32-33	38.48716398856179	40.0	38.0	41.0	34.0	41.0
34-35	38.299466682278705	40.0	38.0	41.0	34.0	41.0
36-37	38.4171792322101	40.0	38.0	41.0	34.0	41.0
38-39	38.536833150045595	40.0	38.0	41.0	34.0	41.0
40-41	38.53208135946902	40.0	38.0	41.0	34.0	41.0
42-43	38.40405523175529	40.0	38.0	41.0	34.0	41.0
44-45	38.34383649896306	40.0	38.0	41.0	34.0	41.0
46-47	38.35964448032293	40.0	38.0	41.0	34.0	41.0
48-49	38.23407775291834	40.0	38.0	41.0	33.0	41.0
50-51	38.17453497917923	40.0	37.5	41.0	33.0	41.0
52-53	38.10026291614276	40.0	37.0	41.0	33.0	41.0
54-55	37.994564425339526	40.0	37.0	41.0	33.0	41.0
56-57	37.84297646671895	40.0	37.0	41.0	33.0	41.0
58-59	37.684903256905784	39.5	36.0	41.0	33.0	41.0
60-61	37.50469642194062	39.0	36.0	41.0	33.0	41.0
62-63	37.285938675829165	39.0	35.5	40.5	32.0	41.0
64-65	37.04390971487584	38.5	35.0	40.0	32.0	41.0
66-67	36.76788693194344	38.0	35.0	40.0	32.0	41.0
68-69	36.45621071239363	37.5	35.0	40.0	31.0	41.0
70-71	36.16095347659183	37.0	35.0	39.5	31.0	41.0
72-73	35.83414220955404	36.5	34.5	39.0	31.0	41.0
74-75	35.506470444550345	36.0	34.0	39.0	31.0	40.0
76-77	34.49533618896713	35.0	33.5	37.0	29.5	39.0
78-79	34.798133620728635	35.0	34.0	37.0	30.0	39.0
80-81	34.64261398308759	35.0	34.0	37.0	30.0	39.0
82-83	34.37982287258643	35.0	34.0	36.0	30.0	38.5
84-85	34.11283447736531	35.0	34.0	36.0	30.0	37.0
86-87	33.866866867434226	35.0	34.0	35.5	30.0	37.0
88-89	33.627783834592705	35.0	33.5	35.0	30.0	36.5
90-91	33.503907670454396	35.0	33.0	35.0	30.0	36.0
92-93	33.42854359660414	35.0	33.0	35.0	30.0	36.0
94-95	33.34288556001174	35.0	33.0	35.0	30.0	36.0
96-97	33.33333585879814	35.0	33.0	35.0	30.0	35.0
98-99	33.36695978820556	34.5	33.0	35.0	30.0	35.0
100-101	32.15374586549361	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	16.0
20	32.0
21	257.0
22	1179.0
23	4230.0
24	11229.0
25	22680.0
26	40273.0
27	63367.0
28	90722.0
29	121458.0
30	155953.0
31	196125.0
32	247050.0
33	320848.0
34	442248.0
35	635872.0
36	1056782.0
37	2038240.0
38	2693285.0
39	1248885.0
40	54915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.998859580623794	21.369058004278795	15.455244574009111	24.176837841088297
2	13.733996862893896	21.91741635936465	41.48655550153891	22.862031276202543
3	16.986330868302655	26.942618401033542	31.299953872648917	24.77109685801489
4	11.943716182767309	19.03339830872018	39.22488795874663	29.79799754976588
5	12.779058207992918	38.22902914687352	35.827447883196534	13.164464761937028
6	29.026310270350503	38.887370843671896	19.002497134388218	13.083821751589387
7	25.71274724060104	33.227436473000374	23.60841107702263	17.451405209375952
8	24.121555574776757	37.08573388065054	21.26515350180915	17.527557042763558
9	25.2969631625931	16.996382237008152	21.598623107270082	36.108031493128664
10-11	23.745011784012647	27.189274116148766	29.205083214879167	19.86063088495942
12-13	24.72662269225659	24.80578615859478	29.34837695043589	21.11921419871274
14-15	21.853912710036866	26.12192713172706	27.12325753481754	24.900902623418535
16-17	21.198276480860105	29.22784974693925	27.766173263665312	21.807700508535337
18-19	21.283038767279656	28.3161839857221	29.661364611801034	20.739412635197212
20-21	22.09645078973388	27.39655093375112	29.619760821064485	20.88723745545052
22-23	21.78859083871011	27.393112205151382	29.442543288740953	21.37575366739756
24-25	21.551088090496272	27.577452970183387	29.475426915193424	21.396032024126914
26-27	21.51421311861973	27.692560004027207	29.42289624913561	21.370330628217456
28-29	21.476821653858607	27.662607291016243	29.369935746040493	21.490635309084656
30-31	21.7336196818812	27.628622542990843	29.326256816478036	21.31150095864992
32-33	21.497163656747166	27.773733469500694	29.269086915540075	21.460015958212065
34-35	21.6898519760833	27.79651393862873	29.26398726811932	21.249646817168646
36-37	21.678092461652813	27.747642191439276	29.179131332560786	21.39513401434713
38-39	21.528302835673394	27.851716339402067	29.229212795631454	21.39076802929309
40-41	21.71888913266857	27.740217103113874	29.069659257600744	21.471234506616813
42-43	21.679591694520237	27.87773885708495	28.798138573346062	21.64453087504875
44-45	21.78106162989847	27.986481927232	28.602004194713487	21.63045224815604
46-47	21.79489225312139	28.016951799706728	28.322322610734176	21.865833336437714
48-49	21.84831020382589	28.12208207282248	28.19240287209795	21.837204851253684
50-51	21.863602092013576	28.231180026903917	28.030765425213637	21.874452455868862
52-53	21.957423863401335	28.15841955291678	27.98349588013374	21.900660703548148
54-55	21.94111146853463	28.120351892624633	27.98913151318809	21.949405125652643
56-57	21.918328694051826	28.201304334663003	27.94865227683223	21.931714694452943
58-59	21.86320125397359	28.252151749618548	27.99234057226205	21.892306424145815
60-61	21.971911405202334	28.251672043454683	27.938826968785907	21.837589582557072
62-63	21.961131376291043	28.31266138133523	27.882015476177223	21.844191766196506
64-65	21.925580385288796	28.274724771837818	27.85256632387037	21.947128519003016
66-67	21.99763120790813	28.24560835477603	27.747560540682194	22.009199896633643
68-69	21.92962255731363	28.200965995162306	27.841801541412835	22.027609906111227
70-71	21.918890867661446	28.04251131111688	27.89068166425291	22.147916156968765
72-73	21.997981641020726	28.045058642347026	27.891372362231877	22.065587354400375
74-75	21.883906123789803	28.163128183542753	27.82114944246892	22.131816250198526
76-77	21.964973564099676	28.090856179799072	27.801535145761296	22.142635110339956
78-79	21.93476556572595	28.050137631018917	27.84018763869624	22.174909164558894
80-81	21.944831564519124	28.059503977484912	27.823565737508943	22.17209872048702
82-83	21.97015117535172	28.05491917795624	27.89282262365568	22.08210702303636
84-85	22.08178591460504	28.040281618754015	27.81439596188269	22.063536504758257
86-87	21.953539351622833	28.040379305605878	27.891590694903996	22.114490647867292
88-89	22.00426928761129	27.948687549457237	27.84243081904907	22.204612343882406
90-91	22.048393787990836	27.953298933169425	27.900616455747134	22.097690823092602
92-93	22.047139491938104	27.895471505858538	27.949799996069586	22.107589006133775
94-95	21.898406240141302	27.92809499921739	28.01388133289093	22.159617427750376
96-97	21.907943918104568	27.970593875332376	28.01618006962826	22.105282136934797
98-99	22.122764673598255	28.54674523351388	28.067950975672705	21.26253911721516
100-101	22.550544589450347	31.283694005860568	25.266706758651917	20.899054646037165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2365.0
1	2131.0
2	2844.5
3	3967.0
4	5272.5
5	7899.0
6	9968.0
7	12087.0
8	14024.5
9	14305.0
10	13165.5
11	12031.0
12	13336.0
13	18424.0
14	30737.0
15	47393.5
16	60426.5
17	66621.5
18	66538.5
19	62201.0
20	55887.5
21	49970.5
22	47082.0
23	49436.5
24	56799.0
25	69990.0
26	87142.0
27	105895.0
28	127351.0
29	151292.0
30	174662.0
31	196890.5
32	219631.0
33	242463.5
34	263928.5
35	282867.0
36	302263.0
37	321911.5
38	337253.0
39	348140.0
40	358092.5
41	368452.5
42	375192.0
43	379310.0
44	384051.0
45	388439.0
46	390023.0
47	388816.0
48	385026.5
49	375411.5
50	364250.5
51	350358.5
52	331267.0
53	311923.5
54	291557.5
55	268449.0
56	245317.0
57	220798.0
58	194136.5
59	166434.0
60	137394.0
61	111731.5
62	89381.0
63	68850.5
64	51930.5
65	39300.5
66	30065.0
67	22718.5
68	16984.0
69	12209.0
70	8512.5
71	6148.5
72	4253.0
73	2871.0
74	2056.0
75	1399.0
76	909.0
77	587.0
78	390.5
79	266.0
80	171.0
81	118.5
82	92.5
83	60.5
84	36.5
85	29.5
86	28.0
87	20.5
88	12.5
89	5.5
90	2.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.246262829188638E-4
4	2.1173772302334368E-4
5	4.02301673744353E-4
6	2.2232460917451084E-4
7	0.0031125445284431517
8	6.246262829188638E-4
9	3.387803568373499E-4
10-11	1.2174919073842259E-4
12-13	3.176065845350155E-5
14-15	1.5350984919192416E-4
16-17	9.528197536050466E-4
18-19	2.1173772302334367E-5
20-21	0.0
22-23	0.002890517825680493
24-25	0.006994799914304434
26-27	0.03591929859001524
28-29	0.27911668489389463
30-31	0.24428490117256646
32-33	0.2516220728343921
34-35	0.13302319470654278
36-37	0.024808935328341494
38-39	0.016053828886641662
40-41	0.009140450856666678
42-43	0.006029551678550795
44-45	0.006566710290612985
46-47	0.0014233606735342707
48-49	0.0020155415659129814
50-51	0.0028540852322721383
52-53	0.0017121110397604687
54-55	2.300593075640452E-4
56-57	0.001751517034141127
58-59	0.0030537557539456776
60-61	0.01324273899263712
62-63	0.00800052241506484
64-65	0.0023301229283976045
66-67	0.0016249256808711446
68-69	0.00224767477432151
70-71	0.004120726138768237
72-73	0.0036435883157684874
74-75	0.0038946500322397486
76-77	0.0015178521091639237
78-79	6.446111666765937E-5
80-81	4.1073387124692745E-4
82-83	1.9787173123320193E-5
84-85	0.0
86-87	6.753295236644244E-6
88-89	0.0
90-91	4.994889950087174E-4
92-93	0.007768659326610075
94-95	9.88097577070179E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	565.0
22-23	1279.0
24-25	3452.0
26-27	4798.0
28-29	8032.0
30-31	18191.0
32-33	24068.0
34-35	34460.0
36-37	48669.0
38-39	58538.0
40-41	62994.0
42-43	75119.0
44-45	89655.0
46-47	89669.0
48-49	78807.0
50-51	69651.0
52-53	66743.0
54-55	71070.0
56-57	74422.0
58-59	74193.0
60-61	73775.0
62-63	72780.0
64-65	77499.0
66-67	82363.0
68-69	83027.0
70-71	81595.0
72-73	78993.0
74-75	80861.0
76-77	81913.0
78-79	86797.0
80-81	89130.0
82-83	87197.0
84-85	88427.0
86-87	93765.0
88-89	99389.0
90-91	107342.0
92-93	112609.0
94-95	126042.0
96-97	188584.0
98-99	803643.0
100-101	5895387.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.76699597660235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74546624709322	76.97199042532746
2	7.261641581575562	12.456183686210391
3	1.7060165260506905	4.389597375774201
4	0.6433913491820509	2.2072697302671083
5	0.26827332305327184	1.1504498509469854
6	0.14083792011238774	0.7247547196579321
7	0.07578709055014164	0.4550021763204677
8	0.045070121925289565	0.309242317266503
9	0.029940607688319014	0.23111243812269774
>10	0.08248257724589994	1.007090409543822
>50	8.532192289064101E-4	0.045723610234118375
>100	2.2091478079200522E-4	0.04179788736574029
>500	1.8521513476874028E-5	0.009785372962606108
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0586886151167184E-5
2	0.0	1.0586886151167184E-5	0.0	1.0586886151167184E-5	1.0586886151167184E-5
3	0.0	1.0586886151167184E-5	0.0	2.1173772302334367E-5	1.0586886151167184E-5
4	0.0	1.0586886151167184E-5	0.0	4.2347544604668735E-5	1.0586886151167184E-5
5	0.0	2.1173772302334367E-5	0.0	6.35213169070031E-5	1.0586886151167184E-5
6	0.0	2.1173772302334367E-5	0.0	6.35213169070031E-5	4.2347544604668735E-5
7	1.0586886151167184E-5	2.1173772302334367E-5	0.0	6.35213169070031E-5	4.2347544604668735E-5
8	1.0586886151167184E-5	2.1173772302334367E-5	0.0	8.469508920933747E-5	4.2347544604668735E-5
9	2.1173772302334367E-5	2.1173772302334367E-5	0.0	9.528197536050464E-5	5.293443075583591E-5
10-11	2.1173772302334367E-5	3.176065845350155E-5	0.0	1.0057541843608823E-4	5.293443075583591E-5
12-13	2.1173772302334367E-5	5.293443075583591E-5	0.0	1.217491907384226E-4	5.293443075583591E-5
14-15	2.6467215377917957E-5	5.8227873831419506E-5	0.0	1.5350984919192416E-4	5.8227873831419506E-5
16-17	3.176065845350155E-5	7.940164613375386E-5	0.0	1.6939017841867494E-4	6.35213169070031E-5
18-19	3.176065845350155E-5	9.528197536050464E-5	0.0	1.7997706456984212E-4	8.469508920933747E-5
20-21	3.176065845350155E-5	1.1116230458725542E-4	0.0	1.8527050764542572E-4	8.469508920933747E-5
22-23	3.176065845350155E-5	1.270426338140062E-4	0.0	1.9585739379659287E-4	8.469508920933747E-5
24-25	3.176065845350155E-5	1.3762951996517338E-4	0.0	2.434983814768452E-4	8.469508920933747E-5
26-27	3.176065845350155E-5	1.3762951996517338E-4	0.0	3.0172625530826475E-4	8.469508920933747E-5
28-29	3.176065845350155E-5	1.3762951996517338E-4	0.0	6.034525106165294E-4	8.469508920933747E-5
30-31	3.176065845350155E-5	1.3762951996517338E-4	0.0	0.0013815886427273173	8.998853228492105E-5
32-33	3.176065845350155E-5	1.4292296304075697E-4	0.0	0.00300667566693148	1.270426338140062E-4
34-35	3.176065845350155E-5	1.5350984919192416E-4	0.0	0.005399311937095263	1.3762951996517338E-4
36-37	3.176065845350155E-5	1.6939017841867494E-4	0.0	0.009231764723817783	1.3762951996517338E-4
38-39	3.176065845350155E-5	1.9056395072100928E-4	0.0	0.015594483300669261	1.3762951996517338E-4
40-41	3.176065845350155E-5	2.487918245524288E-4	0.0	0.0221636461574685	1.3762951996517338E-4
42-43	4.2347544604668735E-5	2.646721537791796E-4	0.0	0.028171704048255874	1.3762951996517338E-4
44-45	4.2347544604668735E-5	2.911393691570975E-4	0.0	0.03556135058177057	1.3762951996517338E-4
46-47	4.2347544604668735E-5	3.0172625530826475E-4	0.0	0.04398851195809965	1.3762951996517338E-4
48-49	4.2347544604668735E-5	3.229000276105991E-4	0.0	0.05188632902687036	1.3762951996517338E-4
50-51	4.2347544604668735E-5	3.3348691376176626E-4	0.0	0.05971003789258292	1.3762951996517338E-4
52-53	4.2347544604668735E-5	3.387803568373499E-4	0.0	0.06748081232753964	1.3762951996517338E-4
54-55	4.764098768025232E-5	4.287688891222709E-4	0.0	0.07604560322383389	1.4821640611634057E-4
56-57	7.940164613375386E-5	4.92290206029274E-4	0.0	0.08266240706831338	1.5880329226750775E-4
58-59	1.0586886151167183E-4	5.134639783316084E-4	0.0	0.08788174194083878	1.5880329226750775E-4
60-61	1.1645574766283901E-4	5.346377506339427E-4	0.0	0.09310107681336421	1.6939017841867494E-4
62-63	1.270426338140062E-4	5.399311937095263E-4	0.0	0.09815102150747096	1.6939017841867494E-4
64-65	1.270426338140062E-4	5.928656244653623E-4	0.0	0.10328566129078703	1.6939017841867494E-4
66-67	1.3762951996517338E-4	6.034525106165294E-4	0.0	0.10884377652014982	1.6939017841867494E-4
68-69	1.5350984919192416E-4	6.140393967676966E-4	0.0	0.11449717372487309	1.7468362149425853E-4
70-71	1.5880329226750775E-4	6.193328398432802E-4	0.0	0.11967945449586942	2.0115083687217647E-4
72-73	1.5880329226750775E-4	6.246262829188638E-4	0.0	0.12370247123331295	2.2232460917451084E-4
74-75	1.5880329226750775E-4	6.246262829188638E-4	0.0	0.12737082728469237	2.2232460917451084E-4
76-77	1.5880329226750775E-4	6.35213169070031E-4	0.0	0.13041455705315294	2.2232460917451084E-4
78-79	1.6939017841867494E-4	6.35213169070031E-4	0.0	0.1330771589201715	2.2232460917451084E-4
80-81	1.7468362149425853E-4	6.35213169070031E-4	0.0	0.13432111804293365	2.2232460917451084E-4
82-83	1.7997706456984212E-4	6.35213169070031E-4	0.0	0.13481340824896293	2.2232460917451084E-4
84-85	1.7997706456984212E-4	6.35213169070031E-4	0.0	0.13512042794734674	2.2232460917451084E-4
86-87	1.7997706456984212E-4	6.35213169070031E-4	0.0	0.13523688369500958	2.2232460917451084E-4
88-89	1.7997706456984212E-4	6.563869413723654E-4	0.0	0.13525805746731193	2.3291149532567802E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12585	0.0	49.052612	1
GTATCAA	23855	0.0	38.074253	1
ATCAACG	29815	0.0	29.832914	3
TCAACGC	30005	0.0	29.829418	4
CAACGCA	30685	0.0	29.12661	5
TATCAAC	30905	0.0	29.001905	2
AACGCAG	31650	0.0	28.292542	6
ACGCAGA	35820	0.0	24.963074	7
CGCAGAG	36765	0.0	24.332403	8
GCAGAGT	41890	0.0	21.161592	9
GTACATG	28790	0.0	20.923048	1
GTGGTAT	6330	0.0	20.517166	1
TACATGG	29060	0.0	20.434734	2
ACATGGG	29705	0.0	19.47327	3
AGAGTAC	38675	0.0	19.341831	10-11
GAGTACT	25415	0.0	19.087101	12-13
TGGTATC	6350	0.0	18.905249	2
TATACCG	840	0.0	18.309547	5
CAGAGTA	40990	0.0	18.20777	10-11
CATGGGG	21250	0.0	18.114246	4
>>END_MODULE