FastQCFastQC Report
Fri 27 May 2016
SRR2139011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9902600
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120050.048.625421
GTATCAA227600.037.5573121
ATCAACG268400.031.2944533
TCAACGC281700.029.8471014
CAACGCA288950.029.0982115
TATCAAC292800.028.9767822
AACGCAG292550.028.8420896
ACGCAGA296100.028.4961537
CGCAGAG346550.024.359518
GCAGAGT403050.020.7126589
GTACATG271800.020.0859551
ACATGGG265600.019.7072413
TACATGG275000.019.4661942
AGAGTAC363100.019.11307510-11
GAGTACT246500.018.76946812-13
GTGGTAT64600.018.0858541
CAGAGTA392800.017.82474910-11
CATGGGG204550.017.2601574
AGTACTT269700.017.19427312-13
TGGTATC62800.017.1837232
Produced by FastQC (version 0.11.5)