##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9902600
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.633148264092256	34.0	31.0	34.0	31.0	34.0
2	32.76294730676792	34.0	31.0	34.0	31.0	34.0
3	33.40380839375518	34.0	31.0	34.0	31.0	37.0
4	36.19646961404076	37.0	35.0	37.0	35.0	37.0
5	36.153486256134755	37.0	35.0	37.0	35.0	37.0
6	36.180148445862706	37.0	36.0	37.0	35.0	37.0
7	36.18841031648254	37.0	36.0	37.0	35.0	37.0
8	36.46675428675297	37.0	37.0	37.0	35.0	39.0
9	37.81964251812655	39.0	38.0	39.0	35.0	39.0
10-11	37.785292801890414	39.0	38.0	39.0	35.0	39.0
12-13	37.922517268192195	39.0	38.0	39.0	35.0	40.0
14-15	39.09969311090017	40.0	38.0	41.0	36.0	41.0
16-17	39.05709308666411	40.0	38.0	41.0	36.0	41.0
18-19	39.01026008321047	40.0	38.5	41.0	35.0	41.0
20-21	39.05193941058069	40.0	39.0	41.0	35.0	41.0
22-23	38.9861351007856	40.0	38.5	41.0	35.0	41.0
24-25	38.90599853520111	40.0	38.0	41.0	35.0	41.0
26-27	38.83058843785429	40.0	38.0	41.0	35.0	41.0
28-29	38.616428844635344	40.0	38.0	41.0	34.5	41.0
30-31	38.56330305226636	40.0	38.0	41.0	34.0	41.0
32-33	38.47580170394785	40.0	38.0	41.0	34.0	41.0
34-35	38.3177519413712	40.0	38.0	41.0	34.0	41.0
36-37	38.45160217078666	40.0	38.0	41.0	34.0	41.0
38-39	38.549666470314875	40.0	38.0	41.0	34.0	41.0
40-41	38.530128425819875	40.0	38.0	41.0	34.0	41.0
42-43	38.41632122452383	40.0	38.0	41.0	34.0	41.0
44-45	38.365658659712864	40.0	38.0	41.0	34.0	41.0
46-47	38.36083571299929	40.0	38.0	41.0	34.0	41.0
48-49	38.25338983485908	40.0	38.0	41.0	33.0	41.0
50-51	38.18026030768933	40.0	37.5	41.0	33.0	41.0
52-53	38.10870101209066	40.0	37.0	41.0	33.0	41.0
54-55	37.999283165778486	40.0	37.0	41.0	33.0	41.0
56-57	37.843467993419495	40.0	37.0	41.0	33.0	41.0
58-59	37.68872465520279	39.5	36.0	41.0	33.0	41.0
60-61	37.50137375903605	39.0	36.0	41.0	33.0	41.0
62-63	37.28218378835177	39.0	35.5	40.5	32.0	41.0
64-65	37.034607238197864	38.5	35.0	40.0	32.0	41.0
66-67	36.75198005178687	38.0	35.0	40.0	32.0	41.0
68-69	36.44196136150742	37.0	35.0	40.0	31.0	41.0
70-71	36.138168672925104	37.0	35.0	39.5	31.0	41.0
72-73	35.80427379186453	36.0	34.5	39.0	31.0	40.5
74-75	35.37635330831106	36.0	34.0	38.5	30.5	40.0
76-77	34.55807774545568	35.0	33.5	37.0	29.5	39.0
78-79	34.78884666577888	35.0	34.0	37.0	30.0	39.0
80-81	34.61200712118713	35.0	34.0	37.0	30.0	39.0
82-83	34.3495774786838	35.0	34.0	36.0	30.0	38.5
84-85	34.08699078800031	35.0	34.0	36.0	30.0	37.0
86-87	33.83862131172702	35.0	34.0	35.5	30.0	37.0
88-89	33.61966738451862	35.0	33.5	35.0	30.0	36.5
90-91	33.50387946762082	35.0	33.0	35.0	30.0	36.0
92-93	33.42878673192463	35.0	33.0	35.0	30.0	36.0
94-95	33.35512638237489	35.0	33.0	35.0	30.0	36.0
96-97	33.34901691128324	35.0	33.0	35.0	30.0	35.0
98-99	33.346883375718875	34.5	33.0	35.0	30.0	35.0
100-101	31.927155846351233	33.0	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	7.0
20	47.0
21	290.0
22	1357.0
23	4680.0
24	11565.0
25	23834.0
26	42064.0
27	65673.0
28	94523.0
29	126312.0
30	161388.0
31	203202.0
32	256543.0
33	331933.0
34	456259.0
35	655699.0
36	1085048.0
37	2104007.0
38	2825493.0
39	1367855.0
40	84817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03081021304218	0.02112577988411722	26.299586381062014	30.648477626011694
2	14.12645415430751	25.123096745422934	37.88082637479113	22.86962272547843
3	15.541088107017423	25.28340969591934	33.89327401654747	25.28222818051576
4	12.66004057331801	24.267949916420662	37.545325368664514	25.526684141596824
5	16.972537191407955	36.91925599814836	32.4662743639117	13.641932446531987
6	27.326844695720016	37.93308202409867	20.75661186356108	13.983461416620239
7	25.430460299130566	33.29864128417807	23.066509869107026	18.20438854758434
8	23.845429478411337	32.41624493122215	22.526686348495613	21.211639241870895
9	23.3935190196975	20.755720531708114	24.321681550335995	31.529078898258394
10-11	24.25522284920727	26.351517223822086	29.143992891558806	20.249267035411837
12-13	24.536540721241177	24.53277908136412	28.95546278975735	21.975217407637352
14-15	21.984485159857524	26.97779321931494	27.021635313653253	24.016086307174284
16-17	21.18135640212417	29.114707767943614	27.994202675213735	21.709733154718478
18-19	21.300067239947346	28.306987799309784	29.603965950139035	20.788979010603835
20-21	22.014748250134225	27.617070629183164	29.471657453533712	20.896523667148898
22-23	21.86367091675278	27.351685952667754	29.26477767329437	21.519865457285096
24-25	21.541780425551174	27.638377681113035	29.42990331100992	21.389938582325872
26-27	21.532648460968755	27.835653892278607	29.245949853233327	21.38574779351931
28-29	21.589696816986482	27.685124863177013	29.217785695780705	21.5073926240558
30-31	21.731666691280292	27.633679786643995	29.247800924569074	21.386852597506632
32-33	21.552040828730128	27.902997486349317	29.12214578482249	21.422815900098062
34-35	21.791271205333945	27.75148671180635	29.023842410790156	21.433399672069545
36-37	21.683536843534714	27.821376089623328	29.048204617547203	21.446882449294755
38-39	21.560357490261868	28.033759335225593	29.05154498793494	21.3543381865776
40-41	21.85211430670016	27.738214607075246	28.826428660373065	21.58324242585153
42-43	21.693455583678816	27.8723083284212	28.75922314469018	21.675012943209804
44-45	21.82891500812388	28.130451142746477	28.41007870918307	21.630555139946573
46-47	21.90465753727367	27.933824162551264	28.222301110822436	21.93921718935263
48-49	21.822720586953636	28.14249608147843	28.223363103817693	21.81142022775024
50-51	21.908086246356046	28.359296238718557	27.90679697696241	21.82582053796299
52-53	22.033583163794095	28.091193934275772	27.889099092339855	21.98612380959028
54-55	21.94537837160654	28.076467113419845	28.004856360163995	21.97329815480962
56-57	21.919382712023886	28.330201333052074	27.87127013036618	21.879145824557856
58-59	21.99264894487078	28.156983065915753	27.921290686186516	21.929077303026954
60-61	21.989991281716435	28.18621810978193	27.94141272785826	21.88237788064337
62-63	21.93024201706162	28.352833550221863	27.860018674766167	21.856905757950347
64-65	22.01358007228752	28.10007210352548	27.813026473608616	22.073321350578382
66-67	21.92383522843258	28.19900246604592	27.81373179249068	22.06343051303082
68-69	21.95152651651719	28.261939098952936	27.81567232074451	21.97086206378536
70-71	22.00966832071223	27.923449520575073	27.823737105555086	22.24314505315762
72-73	21.996819986968575	28.06304870520807	27.79991530112655	22.140216006696807
74-75	21.907778154717704	28.189659672499896	27.79840046934875	22.10416170343365
76-77	22.083140985893447	27.972318129820305	27.685439103417796	22.259101780868452
78-79	21.91001722443672	28.04012484512464	27.82900955742937	22.220848373009268
80-81	22.003672558268452	28.14762985038419	27.698531195976013	22.15016639537134
82-83	22.10974829981837	27.9393364948143	27.809747817911074	22.141167387456257
84-85	22.132689826279794	27.947269322541008	27.85360069050611	22.06644016067309
86-87	22.021145524165313	27.99788520055784	27.80799399649705	22.172975278779802
88-89	22.075878974374923	27.821754631430238	27.830374126963058	22.271992267231774
90-91	22.102090394989098	27.90059774290034	27.846286658477432	22.151025203633132
92-93	22.085386770898324	27.881698898480657	27.897092886754592	22.135821443866426
94-95	22.033211168729547	27.809373449037178	27.92156811241171	22.235847269821566
96-97	21.88684199128556	27.987996288353894	28.03475573769461	22.090405982665935
98-99	22.30550699414999	28.916764717244103	27.895663874326694	20.882064414279213
100-101	22.654012643959664	31.021135782753028	24.843563735466294	21.481287837821007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2082.0
1	1869.5
2	2660.0
3	3876.5
4	5217.0
5	7882.5
6	9941.5
7	11879.0
8	13557.0
9	13726.5
10	12703.5
11	11705.5
12	13048.5
13	17255.5
14	27902.0
15	43659.5
16	57089.0
17	64389.0
18	64782.5
19	60546.5
20	54681.0
21	49454.5
22	47359.5
23	50700.5
24	59359.5
25	73155.0
26	91412.0
27	112227.5
28	135452.0
29	160484.0
30	185248.0
31	209005.5
32	233091.5
33	256701.5
34	279198.5
35	299063.0
36	319870.5
37	339964.5
38	354970.0
39	367059.0
40	377564.5
41	387256.0
42	392370.5
43	396906.0
44	403229.0
45	407387.0
46	408837.5
47	407344.5
48	404233.5
49	397198.0
50	385950.5
51	371662.5
52	353423.0
53	333129.5
54	312259.5
55	289101.5
56	264151.0
57	237615.5
58	209771.0
59	181044.5
60	150626.0
61	122089.5
62	96952.0
63	74952.0
64	56064.5
65	41481.5
66	31076.5
67	23310.5
68	17480.0
69	12712.5
70	8950.5
71	6475.5
72	4618.5
73	3179.5
74	2205.0
75	1540.0
76	1052.0
77	657.5
78	404.0
79	265.0
80	181.5
81	127.5
82	103.5
83	76.5
84	46.0
85	25.0
86	15.0
87	12.5
88	11.5
89	11.0
90	7.5
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.068850604891645E-5
2	2.3226223416072546E-4
3	6.361965544402481E-4
4	3.3324581423060614E-4
5	4.4432775230747484E-4
6	5.958031224122958E-4
7	0.002373114131642195
8	6.563932704542241E-4
9	2.4236059216771352E-4
10-11	7.068850604891645E-5
12-13	4.0393432027952256E-5
14-15	1.6662290711530307E-4
16-17	0.0010249833377092884
18-19	4.544261103144629E-5
20-21	2.019736970664179E-4
22-23	0.0032167129294286513
24-25	0.01110692728296223
26-27	0.0715080420525092
28-29	0.27197646376193724
30-31	0.24558243968612223
32-33	0.24438851121841135
34-35	0.10841301373470695
36-37	0.02383052905461347
38-39	0.015373362415553194
40-41	0.008616448349670689
42-43	0.006030765452168498
44-45	0.006815230631138211
46-47	0.0015563818721563497
48-49	0.0020272658678202275
50-51	0.002639703180523188
52-53	0.0013576767871020486
54-55	2.7485436566580834E-4
56-57	0.0015471220950495253
58-59	0.003379629785057784
60-61	0.01283380744455688
62-63	0.00832313213191589
64-65	0.0021734083147702415
66-67	0.0021022769343343344
68-69	0.002722746481319612
70-71	0.004032455574496237
72-73	0.0038830294892719624
74-75	0.0035304069469204225
76-77	0.0013589885060792393
78-79	1.3002361724217184E-4
80-81	3.195262090433185E-4
82-83	6.340885094982336E-6
84-85	0.0
86-87	1.3001347394637243E-5
88-89	6.589824046426101E-5
90-91	0.001578085896820197
92-93	0.007200552785078715
94-95	7.949161311639266E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	171.0
20-21	735.0
22-23	1456.0
24-25	3700.0
26-27	5429.0
28-29	10040.0
30-31	19943.0
32-33	27301.0
34-35	39564.0
36-37	54118.0
38-39	62306.0
40-41	66643.0
42-43	80189.0
44-45	94290.0
46-47	92648.0
48-49	81896.0
50-51	74063.0
52-53	73123.0
54-55	78065.0
56-57	80855.0
58-59	80718.0
60-61	78997.0
62-63	79438.0
64-65	85400.0
66-67	88269.0
68-69	89367.0
70-71	88136.0
72-73	86824.0
74-75	88684.0
76-77	91131.0
78-79	94522.0
80-81	95506.0
82-83	94895.0
84-85	97214.0
86-87	102804.0
88-89	108371.0
90-91	115591.0
92-93	122879.0
94-95	141958.0
96-97	254771.0
98-99	994129.0
100-101	5876461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.08312058204204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63696869675428	73.64235958259121
2	7.571489761249824	12.581259936392316
3	2.071028083617505	5.162024279999658
4	0.7879281749114762	2.6185412626463394
5	0.3593700324711673	1.4928791870687175
6	0.1964794444159974	0.9794475223384163
7	0.10624412667911728	0.6178965511610396
8	0.06997318369525929	0.46508723667700835
9	0.044813331903603766	0.3350908312407165
>10	0.15309997153988855	1.905998381552945
>50	0.0021279778763308253	0.11651038690977929
>100	4.352192779273808E-4	0.05999875123894531
>500	4.199560764447088E-5	0.022906090182924384
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.0393432027952256E-5	0.0	0.0	0.0
3	0.0	4.0393432027952256E-5	0.0	1.0098358006988064E-5	0.0
4	0.0	4.0393432027952256E-5	0.0	2.0196716013976128E-5	0.0
5	0.0	4.0393432027952256E-5	0.0	2.0196716013976128E-5	0.0
6	5.049179003494032E-5	4.0393432027952256E-5	0.0	2.0196716013976128E-5	0.0
7	5.049179003494032E-5	4.0393432027952256E-5	0.0	2.0196716013976128E-5	0.0
8	5.049179003494032E-5	5.049179003494032E-5	0.0	4.0393432027952256E-5	1.0098358006988064E-5
9	6.059014804192838E-5	1.0098358006988064E-4	0.0	4.0393432027952256E-5	1.0098358006988064E-5
10-11	6.563932704542241E-5	1.0098358006988064E-4	0.0	5.049179003494032E-5	1.0098358006988064E-5
12-13	7.068850604891645E-5	1.0098358006988064E-4	0.0	7.068850604891645E-5	1.0098358006988064E-5
14-15	7.068850604891645E-5	1.716720861187971E-4	0.0	8.078686405590451E-5	1.5147537010482095E-5
16-17	1.3127865409084483E-4	1.9186880213277322E-4	0.0	9.088522206289258E-5	2.0196716013976128E-5
18-19	1.3632783309433885E-4	1.9186880213277322E-4	0.0	9.593440106638661E-5	2.0196716013976128E-5
20-21	1.413770120978329E-4	1.9691798113626724E-4	0.0	1.110819380768687E-4	2.0196716013976128E-5
22-23	1.413770120978329E-4	2.3226223416072546E-4	0.0	1.3127865409084483E-4	2.5245895017470158E-5
24-25	1.4642619110132694E-4	2.3226223416072546E-4	0.0	1.3632783309433885E-4	3.029507402096419E-5
26-27	1.5147537010482096E-4	2.3226223416072546E-4	0.0	2.4236059216771352E-4	3.029507402096419E-5
28-29	1.5652454910831498E-4	2.3226223416072546E-4	0.0	5.756064063983196E-4	3.029507402096419E-5
30-31	1.6157372811180902E-4	2.4236059216771352E-4	0.0	0.0016359339971320664	3.029507402096419E-5
32-33	1.6157372811180902E-4	2.4236059216771352E-4	0.0	0.0038272776846484764	3.029507402096419E-5
34-35	1.8177044412578516E-4	2.6255730818168965E-4	0.0	0.006604326136570193	4.0393432027952256E-5
36-37	1.868196231292792E-4	2.827540241956658E-4	0.0	0.010891079110536626	4.0393432027952256E-5
38-39	1.9186880213277322E-4	3.029507402096419E-4	0.0	0.016808716902631634	4.0393432027952256E-5
40-41	1.9186880213277322E-4	3.332458142306061E-4	0.0	0.023549370872296166	4.0393432027952256E-5
42-43	1.9186880213277322E-4	4.1403267828651056E-4	0.0	0.02974471350958334	5.0491790034940315E-5
44-45	1.9186880213277322E-4	4.948195423424151E-4	0.0	0.037575990144002584	6.059014804192838E-5
46-47	1.9186880213277322E-4	4.948195423424151E-4	0.0	0.046215135418980875	8.078686405590451E-5
48-49	2.2216387615373742E-4	4.948195423424151E-4	0.0	0.05455637913275302	8.078686405590451E-5
50-51	2.6255730818168965E-4	6.412457334437421E-4	0.0	0.06262496718033647	8.078686405590451E-5
52-53	2.7265566618867775E-4	6.462949124472361E-4	0.0	0.07109748954819946	8.078686405590451E-5
54-55	2.7265566618867775E-4	6.513440914507301E-4	0.0	0.07966594631712884	8.078686405590451E-5
56-57	2.7265566618867775E-4	6.563932704542241E-4	0.0	0.08634601013875144	8.078686405590451E-5
58-59	2.7265566618867775E-4	6.614424494577182E-4	0.0	0.09198594308565428	8.078686405590451E-5
60-61	2.878032031991598E-4	6.715408074647062E-4	0.0	0.09757033506351867	8.078686405590451E-5
62-63	2.928523822026538E-4	6.816391654716943E-4	0.0	0.10290731727021186	9.088522206289258E-5
64-65	3.029507402096419E-4	8.381637145800093E-4	0.0	0.10824429947690506	9.088522206289258E-5
66-67	3.2314745622361805E-4	8.482620725869973E-4	0.0	0.11366711772665765	9.593440106638661E-5
68-69	3.2314745622361805E-4	8.785571466079615E-4	0.0	0.11950901783370024	1.0098358006988064E-4
70-71	3.2819663522711207E-4	8.987538626219377E-4	0.0	0.12490154100943188	1.110819380768687E-4
72-73	3.534425302445822E-4	8.987538626219377E-4	0.0	0.12931452345848565	1.2118029608385676E-4
74-75	3.5849170924807624E-4	9.038030416254317E-4	0.0	0.13275301435986508	1.2118029608385676E-4
76-77	3.635408882515703E-4	9.340981156463958E-4	0.0	0.1360400298911397	1.262294750873508E-4
78-79	3.635408882515703E-4	9.59344010663866E-4	0.0	0.13888271767010685	1.3127865409084483E-4
80-81	3.635408882515703E-4	9.59344010663866E-4	0.0	0.1402005533900188	1.3632783309433885E-4
82-83	3.635408882515703E-4	9.59344010663866E-4	0.0	0.1406347827843193	1.413770120978329E-4
84-85	3.635408882515703E-4	9.59344010663866E-4	0.0	0.14089229091349748	1.413770120978329E-4
86-87	3.786884252620524E-4	9.896390846848302E-4	0.0	0.14097812695655687	1.413770120978329E-4
88-89	3.938359622725345E-4	0.0010249833377092886	0.0	0.14099327449356736	1.413770120978329E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12005	0.0	48.62542	1
GTATCAA	22760	0.0	37.557312	1
ATCAACG	26840	0.0	31.294453	3
TCAACGC	28170	0.0	29.847101	4
CAACGCA	28895	0.0	29.098211	5
TATCAAC	29280	0.0	28.976782	2
AACGCAG	29255	0.0	28.842089	6
ACGCAGA	29610	0.0	28.496153	7
CGCAGAG	34655	0.0	24.35951	8
GCAGAGT	40305	0.0	20.712658	9
GTACATG	27180	0.0	20.085955	1
ACATGGG	26560	0.0	19.707241	3
TACATGG	27500	0.0	19.466194	2
AGAGTAC	36310	0.0	19.113075	10-11
GAGTACT	24650	0.0	18.769468	12-13
GTGGTAT	6460	0.0	18.085854	1
CAGAGTA	39280	0.0	17.824749	10-11
CATGGGG	20455	0.0	17.260157	4
AGTACTT	26970	0.0	17.194273	12-13
TGGTATC	6280	0.0	17.183723	2
>>END_MODULE