FastQCFastQC Report
Fri 27 May 2016
SRR2139010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11101271
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146700.047.4642751
GTATCAA263700.035.450311
ATCAACG317150.028.6927813
TCAACGC318150.028.6832264
CAACGCA326300.027.9798035
TATCAAC333650.027.5689092
AACGCAG333900.027.4840186
ACGCAGA382450.023.9166897
CGCAGAG384400.023.7835838
GCAGAGT448450.020.27219
GTACATG288150.020.0776621
TACATGG289100.019.5080642
GAGTACT282350.019.01092312-13
AGAGTAC412200.018.82928310-11
ACATGGG295600.018.354843
CAGAGTA446750.017.74212310-11
AGTACTT314600.017.17098412-13
GTACTTT320700.016.77814314-15
GTGGTAT75700.016.4050881
CATGGGG211000.016.2158894
Produced by FastQC (version 0.11.5)