##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11101271
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49990483071713	34.0	31.0	34.0	31.0	34.0
2	32.65232989988264	34.0	31.0	34.0	31.0	34.0
3	32.6450764961958	34.0	31.0	34.0	31.0	34.0
4	36.20044164312357	37.0	35.0	37.0	35.0	37.0
5	36.13945223028967	37.0	35.0	37.0	35.0	37.0
6	36.164435225480034	37.0	36.0	37.0	35.0	37.0
7	36.11646143941536	37.0	36.0	37.0	35.0	37.0
8	36.102429262379054	37.0	36.0	37.0	35.0	37.0
9	37.812510297244344	39.0	38.0	39.0	35.0	39.0
10-11	37.755779135560246	39.0	38.0	39.0	35.0	39.0
12-13	37.72739076453498	39.0	38.0	39.0	35.0	39.0
14-15	39.077778121081806	40.0	38.0	41.0	36.0	41.0
16-17	39.05599642599482	40.0	38.0	41.0	36.0	41.0
18-19	38.97746703958492	40.0	38.5	41.0	35.0	41.0
20-21	39.03816124791097	40.0	39.0	41.0	35.0	41.0
22-23	38.967565769095316	40.0	38.0	41.0	35.0	41.0
24-25	38.89809684111435	40.0	38.0	41.0	35.0	41.0
26-27	38.822112164072934	40.0	38.0	41.0	35.0	41.0
28-29	38.607989634791615	40.0	38.0	41.0	34.5	41.0
30-31	38.54074945568412	40.0	38.0	41.0	34.0	41.0
32-33	38.479559295192715	40.0	38.0	41.0	34.0	41.0
34-35	38.293497481587245	40.0	38.0	41.0	34.0	41.0
36-37	38.41072557123069	40.0	38.0	41.0	34.0	41.0
38-39	38.53421623626056	40.0	38.0	41.0	34.0	41.0
40-41	38.53355096290829	40.0	38.0	41.0	34.0	41.0
42-43	38.40858597809184	40.0	38.0	41.0	34.0	41.0
44-45	38.35063046722317	40.0	38.0	41.0	34.0	41.0
46-47	38.37436453045923	40.0	38.0	41.0	34.0	41.0
48-49	38.25545219962447	40.0	38.0	41.0	33.0	41.0
50-51	38.200420130403266	40.0	38.0	41.0	33.0	41.0
52-53	38.13559458557599	40.0	37.0	41.0	33.0	41.0
54-55	38.03916119628798	40.0	37.0	41.0	33.0	41.0
56-57	37.89468926981506	40.0	37.0	41.0	33.0	41.0
58-59	37.74797799391763	40.0	36.5	41.0	33.0	41.0
60-61	37.57830364326237	39.0	36.0	41.0	33.0	41.0
62-63	37.372970744416286	39.0	36.0	41.0	32.0	41.0
64-65	37.13964345093379	39.0	35.0	40.0	32.0	41.0
66-67	36.873347731429625	38.0	35.0	40.0	32.0	41.0
68-69	36.56914239923684	37.5	35.0	40.0	31.0	41.0
70-71	36.27674017674694	37.0	35.0	40.0	31.0	41.0
72-73	35.94937211238151	36.5	35.0	39.0	31.0	41.0
74-75	35.6184586837503	36.0	34.0	39.0	31.0	40.0
76-77	34.59598587440857	35.0	33.5	37.0	29.5	39.0
78-79	34.8982076321895	35.0	34.0	37.0	30.0	39.0
80-81	34.73222838828701	35.0	34.0	37.0	30.0	39.0
82-83	34.45827635396181	35.0	34.0	36.5	30.0	39.0
84-85	34.178371175662946	35.0	34.0	36.0	30.0	37.5
86-87	33.91655669848031	35.0	34.0	36.0	30.0	37.0
88-89	33.66445944403049	35.0	33.5	35.0	30.0	37.0
90-91	33.53233027110579	35.0	33.0	35.0	30.0	36.0
92-93	33.45274764270792	35.0	33.0	35.0	30.0	36.0
94-95	33.359174831719336	35.0	33.0	35.0	30.0	36.0
96-97	33.34341157267018	35.0	33.0	35.0	30.0	35.5
98-99	33.375075413866696	34.5	33.0	35.0	30.0	35.0
100-101	32.16818646745347	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	9.0
20	49.0
21	337.0
22	1447.0
23	5138.0
24	13371.0
25	27047.0
26	47451.0
27	74767.0
28	106717.0
29	142743.0
30	183840.0
31	230652.0
32	290474.0
33	374302.0
34	513143.0
35	734472.0
36	1209324.0
37	2349868.0
38	3235106.0
39	1497429.0
40	63584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.145740249021934	22.12596197318307	15.666413332311228	24.061884445483763
2	13.441821211282926	22.367781130647113	41.69735159154298	22.49304606652698
3	16.63032072318714	27.39701039338745	31.670163738257678	24.30250514516773
4	11.725765395512976	19.429399597558977	39.893979545013565	28.950855461914482
5	12.511705912174762	38.44357579179529	36.29540803954301	12.74931025648694
6	28.400380894077777	39.56661993618916	19.289126836088442	12.743872333644621
7	25.129984323869824	33.51703826535989	24.2383042034045	17.114673207365783
8	23.548865916826756	37.550667881909135	21.717876981742716	17.182589219521397
9	24.907508071846753	17.214294728723523	22.154653187810837	35.723544011618884
10-11	23.299337498745636	27.515822075003726	29.74077631894464	19.444064107305998
12-13	24.26583161491102	25.155639878255347	29.81543639879697	20.76309210803667
14-15	21.406988289074985	26.525534313318367	27.527853149616018	24.53962424799063
16-17	20.81260692000746	29.645443774275027	28.19164695478577	21.350302350931745
18-19	20.91230835467136	28.832038428761543	29.937709370780464	20.317943845786633
20-21	21.648696500671946	27.94066505416138	29.88448146151154	20.52615698365513
22-23	21.36847950360552	27.90299989986234	29.70169383994849	21.026826756583645
24-25	21.175077631320818	28.062495792782972	29.716676655850232	21.04574992004598
26-27	21.154500781582165	28.16174670425716	29.664386319358936	21.019366194801744
28-29	21.11846245815151	28.17988746157599	29.596713722814787	21.104936357457714
30-31	21.299511511684553	28.13522544021986	29.586218334750143	20.979044713345445
32-33	21.150700181854933	28.236794296431434	29.51030936015593	21.1021961615577
34-35	21.3191928585895	28.271183238864705	29.45725116727259	20.952372735273205
36-37	21.324053983711053	28.24453038728371	29.410714490614744	21.020701138390496
38-39	21.201380986483777	28.338836336369322	29.441935812211028	21.017846864935873
40-41	21.345691869921957	28.23476947413473	29.32998433454117	21.089554321402147
42-43	21.34008772610297	28.3627636641881	29.02630735795807	21.27084125175086
44-45	21.464486379559432	28.461988801615302	28.843416173086787	21.230108645738476
46-47	21.415366349307973	28.493042256166063	28.63668155768612	21.454909836839846
48-49	21.42484246505849	28.63559597729342	28.496597077748966	21.442964479899125
50-51	21.479506971741216	28.62729886587764	28.398852779988704	21.494341382392438
52-53	21.534923621947833	28.610747353928318	28.307234332817554	21.5470946913063
54-55	21.540060012533942	28.545066186971596	28.337707745622133	21.577166054872333
56-57	21.55499855194994	28.616965372207794	28.29062133967169	21.537414736170582
58-59	21.50308975294754	28.690689020800846	28.303859499468643	21.502361726782972
60-61	21.59432013093525	28.65688855326678	28.297298196522974	21.451493119274993
62-63	21.575936453458564	28.716156185313213	28.227421507298274	21.480485853929952
64-65	21.542646795030432	28.663065190911922	28.203518781724856	21.59076923233279
66-67	21.59123855892583	28.64921713269502	28.109734590265557	21.649809718113598
68-69	21.526110180647027	28.62580096034945	28.184655648567432	21.663433210436096
70-71	21.51798503941045	28.49056880165657	28.224175509123572	21.767270649809408
72-73	21.602878488137538	28.547612048457065	28.192117516603936	21.657391946801457
74-75	21.510413837752193	28.59876069669808	28.161480991882286	21.729344473667446
76-77	21.58213153733857	28.559787874124776	28.109966321055712	21.748114267480943
78-79	21.54443702053165	28.534683393602844	28.117221755156063	21.80365783070944
80-81	21.568817977791007	28.571313681354727	28.09936807862554	21.760500262228724
82-83	21.568023876157145	28.589038364348628	28.191375413492864	21.651562346001366
84-85	21.644589066875564	28.583616165510794	28.136966080594927	21.63482868701871
86-87	21.570469435825704	28.59441957289675	28.15877019739234	21.676340793885203
88-89	21.58527270464291	28.532425179382642	28.110679963232588	21.77162215274186
90-91	21.57092935550088	28.59977953931375	28.15513424786381	21.67415685732156
92-93	21.615335496061356	28.53781994329627	28.181260367098908	21.66558419354346
94-95	21.45606607049745	28.667540786277755	28.185883411056555	21.690509732168238
96-97	21.408291848643167	28.709580900994524	28.22098708731301	21.6611401630493
98-99	21.658327349214627	29.25575933243765	28.263474327842385	20.82243899050534
100-101	22.071211143694804	32.018340204169185	25.55372751406231	20.356721138073702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3626.0
1	3024.0
2	3532.0
3	4911.5
4	6568.0
5	9573.5
6	11907.0
7	14069.5
8	15739.5
9	15784.5
10	14556.5
11	13522.0
12	15231.5
13	20693.0
14	33554.5
15	51081.5
16	64674.5
17	71412.0
18	72211.5
19	69091.0
20	64086.0
21	59390.0
22	58133.0
23	63880.0
24	76357.0
25	94721.0
26	117957.5
27	142862.0
28	170015.0
29	199270.5
30	227128.5
31	254999.5
32	284574.0
33	313904.5
34	339314.0
35	360272.0
36	383944.5
37	407116.0
38	422277.0
39	431908.5
40	440996.0
41	449278.5
42	452892.0
43	455875.5
44	457902.0
45	456533.0
46	452901.0
47	446236.0
48	437142.0
49	424868.5
50	408665.0
51	386618.5
52	360441.5
53	334392.5
54	309129.5
55	281744.0
56	253752.5
57	226358.0
58	198066.5
59	168434.5
60	137649.5
61	110272.5
62	86422.5
63	65432.5
64	48350.0
65	36043.0
66	27374.0
67	20613.0
68	15618.5
69	11241.5
70	7841.0
71	5606.5
72	3902.5
73	2609.5
74	1712.0
75	1140.0
76	731.5
77	472.0
78	310.5
79	215.0
80	165.0
81	116.5
82	82.0
83	52.5
84	32.5
85	25.0
86	21.0
87	21.5
88	22.0
89	17.5
90	11.0
91	7.0
92	4.5
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.026222492901939E-4
4	1.5313561843504226E-4
5	3.873430348651069E-4
6	1.9817550621005467E-4
7	0.0028555288849357882
8	5.134547206351417E-4
9	2.882552817600795E-4
10-11	8.107179799502237E-5
12-13	2.7023932665007456E-5
14-15	1.1710370821503231E-4
16-17	8.827818003902437E-4
18-19	2.2519943887506213E-5
20-21	0.0
22-23	0.0029684550970491123
24-25	0.007208633997197463
26-27	0.03586732030102949
28-29	0.2793088117068706
30-31	0.24356124432425513
32-33	0.2504611538255742
34-35	0.13118614414548668
36-37	0.02262499673371526
38-39	0.014336690565574435
40-41	0.007770207707134612
42-43	0.004582856493061977
44-45	0.005601354714967194
46-47	0.0012214237537775713
48-49	0.0017940853526826786
50-51	0.0024323619861257497
52-53	0.0012953948904985298
54-55	1.2175481414882499E-4
56-57	0.001365047210010451
58-59	0.0026842148948768344
60-61	0.011388894688788962
62-63	0.006856294683041709
64-65	0.0017581911891653144
66-67	0.001631224802578197
68-69	0.0024399263960717494
70-71	0.004148571596576994
72-73	0.003576690396977237
74-75	0.003830725878804796
76-77	0.0015576639654222315
78-79	8.170776194868436E-5
80-81	3.5253816184288887E-4
82-83	2.7857291774265455E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	4.976910939115574E-4
92-93	0.008008728801450364
94-95	9.841597971205899E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	197.0
20-21	710.0
22-23	1559.0
24-25	3917.0
26-27	5617.0
28-29	8883.0
30-31	19876.0
32-33	26741.0
34-35	38083.0
36-37	53403.0
38-39	63653.0
40-41	69212.0
42-43	82626.0
44-45	99156.0
46-47	98427.0
48-49	87236.0
50-51	78821.0
52-53	76550.0
54-55	82226.0
56-57	86586.0
58-59	86472.0
60-61	85206.0
62-63	84605.0
64-65	90459.0
66-67	95374.0
68-69	94509.0
70-71	94128.0
72-73	92002.0
74-75	94717.0
76-77	96082.0
78-79	101500.0
80-81	103039.0
82-83	101585.0
84-85	103676.0
86-87	108847.0
88-89	115896.0
90-91	122294.0
92-93	131017.0
94-95	148845.0
96-97	228592.0
98-99	946534.0
100-101	6992413.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.25228082506794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.95701414727486	74.94831337295744
2	7.5507188153713045	12.72330564127575
3	1.9546012866071039	4.940388495007826
4	0.7179261875140819	2.4194767504842734
5	0.34855425594456757	1.468324552730716
6	0.16606768613532322	0.8394948796945497
7	0.09792685189375896	0.5775392438247472
8	0.05975870414927407	0.4027845698981434
9	0.03807467423079155	0.2887090331054065
>10	0.10849034792487824	1.3197916648712156
>50	7.392257843442076E-4	0.042526122336384135
>100	1.1073638606379322E-4	0.02171847036964044
>500	1.7080783752349644E-5	0.007627203443900666
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.007977555002485E-6	9.007977555002485E-6
2	0.0	9.007977555002485E-6	0.0	9.007977555002485E-6	1.801595511000497E-5
3	0.0	9.007977555002485E-6	0.0	2.702393266500746E-5	1.801595511000497E-5
4	0.0	3.603191022000994E-5	0.0	2.702393266500746E-5	2.702393266500746E-5
5	0.0	3.603191022000994E-5	0.0	2.702393266500746E-5	2.702393266500746E-5
6	6.30558428850174E-5	3.603191022000994E-5	0.0	2.702393266500746E-5	2.702393266500746E-5
7	6.30558428850174E-5	3.603191022000994E-5	0.0	2.702393266500746E-5	2.702393266500746E-5
8	6.30558428850174E-5	5.404786533001492E-5	0.0	2.702393266500746E-5	2.702393266500746E-5
9	6.30558428850174E-5	1.0809573066002984E-4	0.0	2.702393266500746E-5	5.404786533001492E-5
10-11	6.755983166251863E-5	1.0809573066002984E-4	0.0	4.5039887775012426E-5	5.404786533001492E-5
12-13	7.206382044001988E-5	1.0809573066002984E-4	0.0	6.755983166251863E-5	5.404786533001492E-5
14-15	7.206382044001988E-5	1.7565556232254848E-4	0.0	1.0809573066002984E-4	6.30558428850174E-5
16-17	1.5313561843504226E-4	1.801595511000497E-4	0.0	1.1710370821503232E-4	6.30558428850174E-5
18-19	1.5313561843504226E-4	1.9367151743255342E-4	0.0	1.48631629657541E-4	6.30558428850174E-5
20-21	1.5313561843504226E-4	2.1619146132005964E-4	0.0	1.6214359599004473E-4	6.30558428850174E-5
22-23	1.6214359599004476E-4	2.3871140520756586E-4	0.0	1.981755062100547E-4	8.557578677252362E-5
24-25	1.7115157354504723E-4	2.6573533787257336E-4	0.0	2.432153939850671E-4	1.1710370821503232E-4
26-27	1.7115157354504723E-4	2.792473042050771E-4	0.0	2.882552817600795E-4	1.261116857700348E-4
28-29	1.7115157354504723E-4	2.792473042050771E-4	0.0	5.224626981901442E-4	1.261116857700348E-4
30-31	1.7115157354504723E-4	3.468071358675957E-4	0.0	0.0013106607342528616	1.261116857700348E-4
32-33	1.7115157354504723E-4	3.5131112464509696E-4	0.0	0.002963624615595818	1.261116857700348E-4
34-35	1.7115157354504723E-4	4.1887095630761557E-4	0.0	0.005283178836008958	1.4412764088003976E-4
36-37	1.7115157354504723E-4	4.954387655251367E-4	0.0	0.008688194351799898	1.5313561843504226E-4
38-39	1.7115157354504723E-4	5.359746645226479E-4	0.0	0.014183060660351413	1.5313561843504226E-4
40-41	1.7115157354504723E-4	5.855185410751616E-4	0.0	0.020281461465088097	1.5313561843504226E-4
42-43	1.7115157354504723E-4	6.170464625176702E-4	0.0	0.026289782494274754	1.6214359599004476E-4
44-45	1.7565556232254848E-4	7.251421931777001E-4	0.0	0.03337005285250671	1.7565556232254848E-4
46-47	1.801595511000497E-4	8.017100023952212E-4	0.0	0.04135112096623891	1.8466353987755095E-4
48-49	1.801595511000497E-4	8.017100023952212E-4	0.0	0.048917822112441	1.891675286550522E-4
50-51	2.432153939850671E-4	9.09805733055251E-4	0.0	0.0565340671351956	1.891675286550522E-4
52-53	2.432153939850671E-4	9.09805733055251E-4	0.0	0.06385755288741263	2.0718348376505717E-4
54-55	2.432153939850671E-4	9.143097218327523E-4	0.0	0.07190167684402984	2.297034276525634E-4
56-57	2.432153939850671E-4	9.27821688165256E-4	0.0	0.07841444461629664	2.3871140520756586E-4
58-59	2.432153939850671E-4	9.368296657202586E-4	0.0	0.0840714545208382	2.4771938276256836E-4
60-61	2.432153939850671E-4	9.63853598385266E-4	0.0	0.08927806554762963	2.522233715400696E-4
62-63	2.792473042050771E-4	0.0010133974749377797	0.0	0.09425046915799101	2.612313490950721E-4
64-65	2.837512929825783E-4	0.0010899652841553009	0.0	0.09952914400522246	2.612313490950721E-4
66-67	2.882552817600795E-4	0.0010899652841553009	0.0	0.10486637070656143	2.6573533787257336E-4
68-69	2.882552817600795E-4	0.0010989732617103032	0.0	0.11034772504878046	2.702393266500746E-4
70-71	2.97263259315082E-4	0.0011124852280428072	0.0	0.11550028821024187	2.792473042050771E-4
72-73	3.062712368700845E-4	0.0011169892168203083	0.0	0.11966197384065302	2.97263259315082E-4
74-75	3.062712368700845E-4	0.001153021127040318	0.0	0.12310752525544147	2.97263259315082E-4
76-77	3.062712368700845E-4	0.001184549048482827	0.0	0.12618374959047482	2.97263259315082E-4
78-79	3.107752256475858E-4	0.0011980610148153307	0.0	0.1289491986998606	2.97263259315082E-4
80-81	3.1978320320258824E-4	0.0011980610148153307	0.0	0.13002114802890588	3.0176724809258325E-4
82-83	3.287911807575907E-4	0.0012025650035928318	0.0	0.13035444319844097	3.15279214425087E-4
84-85	3.5131112464509696E-4	0.0012160769699253355	0.0	0.13065170645775606	3.15279214425087E-4
86-87	3.603191022000994E-4	0.0012521088801453455	0.0	0.1307958340986361	3.2428719198008947E-4
88-89	3.603191022000994E-4	0.0013016527566978593	0.0	0.1308318660088561	3.2428719198008947E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14670	0.0	47.464275	1
GTATCAA	26370	0.0	35.45031	1
ATCAACG	31715	0.0	28.692781	3
TCAACGC	31815	0.0	28.683226	4
CAACGCA	32630	0.0	27.979803	5
TATCAAC	33365	0.0	27.568909	2
AACGCAG	33390	0.0	27.484018	6
ACGCAGA	38245	0.0	23.916689	7
CGCAGAG	38440	0.0	23.783583	8
GCAGAGT	44845	0.0	20.2721	9
GTACATG	28815	0.0	20.077662	1
TACATGG	28910	0.0	19.508064	2
GAGTACT	28235	0.0	19.010923	12-13
AGAGTAC	41220	0.0	18.829283	10-11
ACATGGG	29560	0.0	18.35484	3
CAGAGTA	44675	0.0	17.742123	10-11
AGTACTT	31460	0.0	17.170984	12-13
GTACTTT	32070	0.0	16.778143	14-15
GTGGTAT	7570	0.0	16.405088	1
CATGGGG	21100	0.0	16.215889	4
>>END_MODULE