FastQCFastQC Report
Fri 27 May 2016
SRR2139009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11892859
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158200.051.1585961
GTATCAA294150.037.5838471
TCAACGC368750.029.1945254
ATCAACG368650.029.1332053
CAACGCA377150.028.578085
TATCAAC383250.028.2902552
AACGCAG389250.027.7005486
ACGCAGA441950.024.358747
CGCAGAG447900.024.0726078
CTTATAC90450.022.0043431
GCAGAGT520200.020.5465569
GTACATG376300.020.0238761
TACATGG366400.019.9368842
GTGGTAT80350.019.837451
ACATGGG370700.019.3607183
TGGTATC76350.019.2580382
AGAGTAC480150.019.18964810-11
GAGTACT313950.019.08619912-13
TATACAC133450.018.87433
CAGAGTA517700.017.98711610-11
Produced by FastQC (version 0.11.5)