##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11892859
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47352474287301	34.0	31.0	34.0	31.0	34.0
2	32.62170332634062	34.0	31.0	34.0	31.0	34.0
3	32.61927203542899	34.0	31.0	34.0	31.0	34.0
4	36.185295226320264	37.0	35.0	37.0	35.0	37.0
5	36.11991414343683	37.0	35.0	37.0	35.0	37.0
6	36.14610910631329	37.0	36.0	37.0	35.0	37.0
7	36.095586015103684	37.0	36.0	37.0	35.0	37.0
8	36.08322515216904	37.0	36.0	37.0	35.0	37.0
9	37.785900093493076	39.0	38.0	39.0	35.0	39.0
10-11	37.72739170623312	39.0	37.5	39.0	35.0	39.0
12-13	37.69669782514028	39.0	37.0	39.0	35.0	39.0
14-15	39.030313106377534	40.0	38.0	41.0	36.0	41.0
16-17	39.004534107400076	40.0	38.0	41.0	36.0	41.0
18-19	38.93234053308797	40.0	38.5	41.0	35.0	41.0
20-21	38.993308928181634	40.0	38.5	41.0	35.0	41.0
22-23	38.92540417511972	40.0	38.0	41.0	35.0	41.0
24-25	38.85204144682058	40.0	38.0	41.0	35.0	41.0
26-27	38.77136883153411	40.0	38.0	41.0	35.0	41.0
28-29	38.559046391281925	40.0	38.0	41.0	34.5	41.0
30-31	38.4849447341189	40.0	38.0	41.0	34.0	41.0
32-33	38.41779300135579	40.0	38.0	41.0	34.0	41.0
34-35	38.22991898888394	40.0	38.0	41.0	34.0	41.0
36-37	38.35364566700081	40.0	38.0	41.0	34.0	41.0
38-39	38.46531216074758	40.0	38.0	41.0	34.0	41.0
40-41	38.46856872426295	40.0	38.0	41.0	34.0	41.0
42-43	38.34714034187033	40.0	38.0	41.0	34.0	41.0
44-45	38.28865230654472	40.0	38.0	41.0	33.0	41.0
46-47	38.30256964077155	40.0	38.0	41.0	33.0	41.0
48-49	38.17893940718423	40.0	38.0	41.0	33.0	41.0
50-51	38.11982449963186	40.0	37.5	41.0	33.0	41.0
52-53	38.05132418590851	40.0	37.0	41.0	33.0	41.0
54-55	37.94496465768043	40.0	37.0	41.0	33.0	41.0
56-57	37.79848874472186	40.0	37.0	41.0	33.0	41.0
58-59	37.64965902245992	39.5	36.0	41.0	33.0	41.0
60-61	37.47898189277588	39.0	36.0	41.0	32.5	41.0
62-63	37.27243652562036	39.0	35.5	40.5	32.0	41.0
64-65	37.043895757599074	38.5	35.0	40.0	32.0	41.0
66-67	36.78042187788166	38.0	35.0	40.0	31.5	41.0
68-69	36.480803220308125	37.5	35.0	40.0	31.0	41.0
70-71	36.19357491666318	37.0	35.0	39.5	31.0	41.0
72-73	35.874462819966936	36.5	34.5	39.0	31.0	41.0
74-75	35.553344936861635	36.0	34.0	39.0	31.0	40.0
76-77	34.52775615530797	35.0	33.5	37.0	29.5	39.0
78-79	34.845739791978765	35.0	34.0	37.0	30.0	39.0
80-81	34.69146600507104	35.0	34.0	37.0	30.0	39.0
82-83	34.42128459243722	35.0	34.0	36.5	30.0	39.0
84-85	34.145051157763575	35.0	34.0	36.0	30.0	37.0
86-87	33.887227905259124	35.0	34.0	36.0	30.0	37.0
88-89	33.639495637549004	35.0	33.0	35.0	30.0	37.0
90-91	33.509464047581204	35.0	33.0	35.0	29.0	36.0
92-93	33.4311128799457	35.0	33.0	35.0	30.0	36.0
94-95	33.33667039050846	35.0	33.0	35.0	29.0	36.0
96-97	33.322634558004324	35.0	33.0	35.0	30.0	35.5
98-99	33.35473993047801	34.0	33.0	35.0	30.0	35.0
100-101	32.14402948465429	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	14.0
20	51.0
21	382.0
22	1747.0
23	5977.0
24	14847.0
25	30317.0
26	52928.0
27	82547.0
28	117233.0
29	157191.0
30	200733.0
31	252699.0
32	318124.0
33	411865.0
34	567256.0
35	805298.0
36	1323347.0
37	2525826.0
38	3386104.0
39	1573185.0
40	65184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.20587631619949	22.06582117891081	15.677037792174279	24.05126471271542
2	13.38272824053493	22.270111837700256	41.93719104884705	22.409968872917773
3	16.554127855323387	27.67254871987102	31.85896895815665	23.91435446664894
4	11.676683059165525	19.498087840766015	39.720746217177094	29.104482882891368
5	12.416986572974164	38.578930706422916	36.22520507146969	12.778877649133236
6	28.25289600693392	39.55422425978789	19.306589569304762	12.886290163973435
7	25.00726926413857	33.664428904915	24.09921246415189	17.22908936679454
8	23.696301891970794	37.47048630497283	21.72435823268967	17.1088535703667
9	25.08231025002674	16.992597542985642	21.900748755045907	36.02434345194171
10-11	23.323030922577626	27.458630071561917	29.661277503137917	19.55706150272254
12-13	24.302452123049488	25.1192575039262	29.718560068511714	20.859730304512603
14-15	21.510270949170717	26.499247719604725	27.404338551781514	24.586142779443044
16-17	20.862891257278594	29.7016291438061	28.020844632233928	21.41463496668138
18-19	20.948288539919382	28.67271085492746	29.91891267169865	20.46008793345451
20-21	21.757657836360774	27.796245546874914	29.81622605390242	20.629870562861893
22-23	21.430328429261085	27.844631570590906	29.654687674235902	21.070352325912108
24-25	21.216870117097137	28.008615981886802	29.725352013154883	21.049161887861178
26-27	21.176986055062553	28.170882303919836	29.646085972736408	21.0060456682812
28-29	20.998737790685354	28.071808725092602	29.657384768834333	21.272068715387714
30-31	21.384711432404842	27.961338285062592	29.704253698682432	20.94969658385013
32-33	21.158554869089706	28.206160545280394	29.517587978847192	21.117696606782708
34-35	21.284810640590216	28.24808168144939	29.6095593300988	20.8575483478616
36-37	21.365093239163745	28.142351751484163	29.432555923826413	21.05999908552568
38-39	21.168398362654404	28.205083134280567	29.50894130444238	21.11757719862265
40-41	21.358789142123648	28.07541814034972	29.415380809341222	21.150411908185415
42-43	21.368449006204077	28.177528426010458	29.17135252283809	21.282670044947377
44-45	21.404904428456046	28.359553165189205	28.930803120105708	21.304739286249042
46-47	21.524135776122506	28.280441766942378	28.595486883230915	21.599935573704197
48-49	21.505941567841116	28.400700966603797	28.550642563863583	21.542714901691507
50-51	21.458329736852704	28.518014411817173	28.47487462668531	21.548781224644813
52-53	21.50368312360054	28.50755790192896	28.44681739880585	21.54194157566465
54-55	21.561742482731855	28.416703305857478	28.3494306002575	21.672123611153165
56-57	21.663528592718393	28.558761308181747	28.242508880330764	21.5352012187691
58-59	21.44580099403143	28.714105357063886	28.401542418628146	21.43855123027654
60-61	21.629679270653327	28.748890040132242	28.21107235314121	21.41035833607322
62-63	21.560955252580083	28.836303839491322	28.125378002718566	21.477362905210033
64-65	21.52005463750039	28.7696115544652	28.135643583489895	21.57469022454451
66-67	21.59410523409592	28.66825159923163	28.085255748006745	21.652387418665707
68-69	21.563475407128845	28.613295816789762	28.163317155586086	21.659911620495308
70-71	21.54727136765523	28.457618614654436	28.194369218694376	21.800740798995957
72-73	21.59974307918046	28.499492537490607	28.170805262128614	21.72995912120032
74-75	21.50386835598347	28.612991484961388	28.145763732517633	21.737376426537512
76-77	21.593082872140563	28.52501641965063	28.080708310634332	21.80119239757447
78-79	21.516668265708944	28.564013025125828	28.058611007367812	21.86070770179742
80-81	21.561523431499975	28.60521923713875	28.07907697123585	21.754180360125424
82-83	21.589319040294637	28.579846347586084	28.155283794590186	21.67555081752909
84-85	21.68206745855508	28.55664361625595	28.075153966604173	21.6861349585848
86-87	21.531754303768107	28.563282981003013	28.159254449203054	21.745708266025826
88-89	21.59353784984674	28.507857652535158	28.095288317130994	21.803316180487112
90-91	21.60735385156595	28.575093941038677	28.13102969936428	21.686522508031096
92-93	21.646574660392492	28.51767936285991	28.163656346916206	21.67208962983139
94-95	21.469488007694235	28.630850904423855	28.155656210532797	21.744004877349116
96-97	21.421050843210498	28.66555473987791	28.212734932385874	21.700659484525715
98-99	21.627528547303694	29.20824691174677	28.28223732035587	20.881987220593665
100-101	22.073283949077375	31.9570487723551	25.504968651364496	20.464698627203028
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3978.0
1	3435.5
2	4101.0
3	5569.0
4	7038.0
5	9913.5
6	12219.5
7	14542.5
8	16754.5
9	17287.0
10	16374.0
11	15365.0
12	16893.5
13	22428.5
14	35884.5
15	54826.0
16	70300.5
17	78497.5
18	80002.5
19	76656.5
20	70975.5
21	65824.0
22	64122.5
23	68782.0
24	80025.0
25	98587.5
26	123908.0
27	152205.5
28	182724.0
29	215939.5
30	248046.0
31	278848.5
32	310868.5
33	341058.0
34	368710.5
35	392552.0
36	416612.0
37	439882.5
38	455790.5
39	465597.5
40	472701.0
41	479363.5
42	481667.0
43	483754.0
44	484780.5
45	483577.5
46	483008.0
47	478694.0
48	465007.0
49	448603.5
50	434787.5
51	414925.5
52	388783.5
53	360930.0
54	335626.5
55	308802.5
56	279258.0
57	248605.0
58	218176.5
59	187516.5
60	154929.5
61	125456.5
62	99255.0
63	76062.0
64	56899.0
65	42825.5
66	32768.0
67	24655.5
68	18514.5
69	13056.5
70	8631.0
71	6008.5
72	4243.0
73	2901.5
74	1887.5
75	1215.5
76	794.5
77	505.5
78	329.5
79	216.5
80	147.0
81	102.5
82	77.5
83	65.5
84	53.5
85	39.0
86	27.5
87	19.0
88	13.5
89	10.0
90	6.5
91	4.0
92	2.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.13813717963023E-4
4	1.7657654900306142E-4
5	4.5405398315072934E-4
6	1.5135132771690977E-4
7	0.0026990986776182246
8	6.306305321537907E-4
9	4.288287618645777E-4
10-11	9.66966815969146E-5
12-13	1.2612610643075814E-5
14-15	1.639639383599856E-4
16-17	9.165163733968427E-4
18-19	1.6816814190767754E-5
20-21	0.0
22-23	0.0027666739407486082
24-25	0.00693934332615595
26-27	0.035409584889935765
28-29	0.27893814310814563
30-31	0.24346028690517368
32-33	0.2509370651225945
34-35	0.1316463892347026
36-37	0.022758849703439686
38-39	0.015056330603323972
40-41	0.008262960453471269
42-43	0.0053828374080155655
44-45	0.006360466661537493
46-47	0.001404007292890712
48-49	0.0018676047420935911
50-51	0.002481510052813007
52-53	0.001463441398890494
54-55	1.4614551480556824E-4
56-57	0.0014236892866782214
58-59	0.0027575313389019036
60-61	0.012288085673021457
62-63	0.008020772711673072
64-65	0.0021907328556260174
66-67	0.0016433585260195373
68-69	0.0025845292521808737
70-71	0.004176428327380442
72-73	0.0037505282284853716
74-75	0.0038491627970153063
76-77	0.0016343013064075151
78-79	8.235404084513364E-5
80-81	5.25884366983355E-4
82-83	1.0537272334328263E-5
84-85	0.0
86-87	5.3964726388314086E-6
88-89	0.0
90-91	5.212450036725593E-4
92-93	0.008106482247197943
94-95	0.0011160726422493248
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	228.0
20-21	767.0
22-23	1701.0
24-25	5016.0
26-27	6360.0
28-29	10029.0
30-31	23194.0
32-33	30372.0
34-35	43093.0
36-37	60746.0
38-39	74286.0
40-41	78744.0
42-43	93555.0
44-45	111591.0
46-47	110650.0
48-49	97641.0
50-51	88209.0
52-53	85811.0
54-55	93855.0
56-57	99875.0
58-59	99251.0
60-61	99956.0
62-63	99591.0
64-65	104329.0
66-67	113131.0
68-69	106311.0
70-71	104157.0
72-73	100706.0
74-75	103100.0
76-77	104957.0
78-79	110493.0
80-81	113352.0
82-83	110794.0
84-85	112576.0
86-87	118369.0
88-89	126433.0
90-91	133542.0
92-93	141958.0
94-95	160739.0
96-97	241598.0
98-99	999547.0
100-101	7372246.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.72202244035572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55472950974121	73.25426321704443
2	7.517854806803678	12.437843080635
3	2.115050034893968	5.248836493469218
4	0.817330901978763	2.7044506085873365
5	0.3728919389097434	1.5423187669159775
6	0.20593580839610207	1.0221255938049092
7	0.12817138015621257	0.7421817049845507
8	0.0783751137755271	0.5186678336403668
9	0.045561196210921896	0.3392022865832398
>10	0.16137995485511913	1.9683385557716415
>50	0.002185416024897996	0.11919351925747718
>100	4.9811640160853E-4	0.08074040865544528
>500	3.5821852278857434E-5	0.02183793065044152
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.408407095383877E-6
2	0.0	2.522522128615163E-5	0.0	0.0	8.408407095383877E-6
3	0.0	2.522522128615163E-5	0.0	8.408407095383877E-6	8.408407095383877E-6
4	0.0	7.56756638584549E-5	0.0	8.408407095383877E-6	8.408407095383877E-6
5	0.0	7.56756638584549E-5	0.0	8.408407095383877E-6	8.408407095383877E-6
6	0.0	7.56756638584549E-5	0.0	8.408407095383877E-6	1.6816814190767754E-5
7	0.0	9.249247804922265E-5	0.0	8.408407095383877E-6	1.6816814190767754E-5
8	0.0	9.249247804922265E-5	0.0	8.408407095383877E-6	3.363362838153551E-5
9	0.0	1.849849560984453E-4	0.0	8.408407095383877E-6	3.363362838153551E-5
10-11	0.0	1.849849560984453E-4	0.0	1.6816814190767754E-5	3.363362838153551E-5
12-13	0.0	1.849849560984453E-4	0.0	2.522522128615163E-5	3.783783192922745E-5
14-15	0.0	2.396396022184405E-4	0.0	3.783783192922745E-5	4.2042035476919384E-5
16-17	0.0	2.4384380576613244E-4	0.0	5.045044257230326E-5	5.46546461199952E-5
18-19	0.0	2.606606199569002E-4	0.0	5.885884966768714E-5	5.885884966768714E-5
20-21	8.408407095383877E-6	2.7747743414766794E-4	0.0	7.147146031076296E-5	5.885884966768714E-5
22-23	8.408407095383877E-6	3.4474469091073896E-4	0.0	9.669668159691458E-5	6.726725676307101E-5
24-25	8.408407095383877E-6	3.6996991219689055E-4	0.0	1.0510508869229846E-4	8.408407095383877E-5
26-27	8.408407095383877E-6	3.867867263876583E-4	0.0	1.3873871707383397E-4	8.408407095383877E-5
28-29	8.408407095383877E-6	3.867867263876583E-4	0.0	2.7747743414766794E-4	8.408407095383877E-5
30-31	8.408407095383877E-6	4.79279204436881E-4	0.0	7.945944705137764E-4	8.828827450153071E-5
32-33	8.408407095383877E-6	4.8768761153226487E-4	0.0	0.002249248898015187	9.249247804922265E-5
34-35	8.408407095383877E-6	5.885884966768714E-4	0.0	0.004338738061218081	1.0090088514460652E-4
36-37	8.408407095383877E-6	6.894893818214779E-4	0.0	0.007163962845267064	1.0510508869229846E-4
38-39	8.408407095383877E-6	7.147146031076296E-4	0.0	0.011889487632872802	1.093092922399904E-4
40-41	8.408407095383877E-6	7.777776563230086E-4	0.0	0.017527324590327693	1.1351349578768235E-4
42-43	8.408407095383877E-6	8.072070811568522E-4	0.0	0.022954951370397984	1.1771769933537428E-4
44-45	8.408407095383877E-6	9.375373911353023E-4	0.0	0.02916456001033898	1.1771769933537428E-4
46-47	8.408407095383877E-6	0.0010426424798276007	0.0	0.03604684121791068	1.219219028830662E-4
48-49	1.2612610643075815E-5	0.0010510508869229847	0.0	0.04285344676162393	1.2612610643075815E-4
50-51	1.6816814190767754E-5	0.0011687685862583588	0.0	0.04977356580112486	1.2612610643075815E-4
52-53	2.1021017738459692E-5	0.0011813811969014348	0.0	0.05658857975193349	1.429429206215259E-4
54-55	2.522522128615163E-5	0.0011897896039968186	0.0	0.06359698706593594	1.639639383599856E-4
56-57	2.522522128615163E-5	0.0012192190288306622	0.0	0.07080719615022762	1.6816814190767754E-4
58-59	2.522522128615163E-5	0.0012192190288306622	0.0	0.07745824616267627	1.6816814190767754E-4
60-61	2.522522128615163E-5	0.001244444250116814	0.0	0.08352911608554343	1.6816814190767754E-4
62-63	2.522522128615163E-5	0.0012822820820460412	0.0	0.0895999860084106	1.6816814190767754E-4
64-65	2.522522128615163E-5	0.0014126123920244914	0.0	0.09603662163992695	1.6816814190767754E-4
66-67	2.522522128615163E-5	0.0014336334097629511	0.0	0.1026834674488279	1.7237234545536948E-4
68-69	2.522522128615163E-5	0.0014798796487875622	0.0	0.10935133427546732	1.7657654900306142E-4
70-71	2.522522128615163E-5	0.0015303300913598655	0.0	0.11551890087993139	1.7657654900306142E-4
72-73	2.522522128615163E-5	0.0015471469055506333	0.0	0.12036214336687251	2.2702699157536467E-4
74-75	2.522522128615163E-5	0.0015891889410275528	0.0	0.12456214271101676	2.3543539867074855E-4
76-77	2.522522128615163E-5	0.0016774772155290834	0.0	0.12820298298331798	2.3543539867074855E-4
78-79	2.522522128615163E-5	0.001715315047458311	0.0	0.13156634582147153	2.3543539867074855E-4
80-81	2.522522128615163E-5	0.001719519251006003	0.0	0.13290328254963757	2.438438057661324E-4
82-83	2.522522128615163E-5	0.0017321318616490787	0.0	0.13331109029376367	2.522522128615163E-4
84-85	2.522522128615163E-5	0.0017405402687444625	0.0	0.13365163078112674	2.522522128615163E-4
86-87	2.522522128615163E-5	0.0017489486758398465	0.0	0.13375253166627132	2.5645641640920823E-4
88-89	2.522522128615163E-5	0.0018120117290552256	0.0	0.13381139051593902	2.648648235045921E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15820	0.0	51.158596	1
GTATCAA	29415	0.0	37.583847	1
TCAACGC	36875	0.0	29.194525	4
ATCAACG	36865	0.0	29.133205	3
CAACGCA	37715	0.0	28.57808	5
TATCAAC	38325	0.0	28.290255	2
AACGCAG	38925	0.0	27.700548	6
ACGCAGA	44195	0.0	24.35874	7
CGCAGAG	44790	0.0	24.072607	8
CTTATAC	9045	0.0	22.004343	1
GCAGAGT	52020	0.0	20.546556	9
GTACATG	37630	0.0	20.023876	1
TACATGG	36640	0.0	19.936884	2
GTGGTAT	8035	0.0	19.83745	1
ACATGGG	37070	0.0	19.360718	3
TGGTATC	7635	0.0	19.258038	2
AGAGTAC	48015	0.0	19.189648	10-11
GAGTACT	31395	0.0	19.086199	12-13
TATACAC	13345	0.0	18.8743	3
CAGAGTA	51770	0.0	17.987116	10-11
>>END_MODULE