FastQCFastQC Report
Fri 27 May 2016
SRR2139008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432101
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC130450.045.535291
GGTATCA214050.039.1591571
TATACAC179300.035.6270873
TTATACA182100.033.687242
GTATCAA350800.031.4813121
ATCAACG421400.025.6356873
TCAACGC423600.025.622124
CAACGCA428600.025.293645
TATCAAC430750.025.28522
TACACAT253450.025.2371585
ACACATC243000.024.9836256
ACATCTC243650.024.8647388
AACGCAG439300.024.7640116
CACATCT264150.022.80737
ATACACA307750.022.0060624
CATCTCC273800.021.848979
ACGCAGA500050.021.7301377
CGCAGAG502500.021.598788
GTACATG409900.020.4695451
TACATGG405000.020.2478682
Produced by FastQC (version 0.11.5)