##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139008_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11432101
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48540158978651	34.0	31.0	34.0	31.0	34.0
2	32.62726615168988	34.0	31.0	34.0	31.0	34.0
3	32.62182681905977	34.0	31.0	34.0	31.0	34.0
4	36.18499801567533	37.0	35.0	37.0	35.0	37.0
5	36.12178128937104	37.0	35.0	37.0	35.0	37.0
6	36.14718659326051	37.0	36.0	37.0	35.0	37.0
7	36.094656878906164	37.0	36.0	37.0	35.0	37.0
8	36.08174193002669	37.0	36.0	37.0	35.0	37.0
9	37.78727182343823	39.0	38.0	39.0	35.0	39.0
10-11	37.72639023220666	39.0	37.5	39.0	35.0	39.0
12-13	37.694138505249384	39.0	37.0	39.0	35.0	39.0
14-15	39.026003575370794	40.0	38.0	41.0	35.5	41.0
16-17	38.9934544840008	40.0	38.0	41.0	35.5	41.0
18-19	38.92230885643855	40.0	38.0	41.0	35.0	41.0
20-21	38.98570859920623	40.0	38.0	41.0	35.0	41.0
22-23	38.917049322793346	40.0	38.0	41.0	35.0	41.0
24-25	38.840749090615006	40.0	38.0	41.0	35.0	41.0
26-27	38.75312649417312	40.0	38.0	41.0	34.5	41.0
28-29	38.52799535406051	40.0	38.0	41.0	34.0	41.0
30-31	38.45424895777123	40.0	38.0	41.0	34.0	41.0
32-33	38.391188072020086	40.0	38.0	41.0	34.0	41.0
34-35	38.199290639066874	40.0	38.0	41.0	34.0	41.0
36-37	38.3261441631883	40.0	38.0	41.0	34.0	41.0
38-39	38.42603872461004	40.0	38.0	41.0	34.0	41.0
40-41	38.44986408181044	40.0	38.0	41.0	34.0	41.0
42-43	38.336285752751735	40.0	38.0	41.0	34.0	41.0
44-45	38.281085299299946	40.0	38.0	41.0	33.0	41.0
46-47	38.29351241891381	40.0	38.0	41.0	33.0	41.0
48-49	38.164934214028605	40.0	38.0	41.0	33.0	41.0
50-51	38.08538760881039	40.0	37.0	41.0	33.0	41.0
52-53	38.02320046707804	40.0	37.0	41.0	33.0	41.0
54-55	37.900315325071276	40.0	37.0	41.0	33.0	41.0
56-57	37.74030666940848	40.0	36.5	41.0	33.0	41.0
58-59	37.58586135715045	39.0	36.0	41.0	33.0	41.0
60-61	37.39837081527926	39.0	36.0	41.0	32.0	41.0
62-63	37.192176074897226	39.0	35.0	40.5	32.0	41.0
64-65	36.97056064691792	38.5	35.0	40.0	32.0	41.0
66-67	36.69993042254589	38.0	35.0	40.0	31.0	41.0
68-69	36.394377684342416	37.0	35.0	40.0	31.0	41.0
70-71	36.102125120366	37.0	35.0	39.5	31.0	41.0
72-73	35.7789987269786	36.0	34.5	39.0	31.0	40.5
74-75	35.45438501336559	36.0	34.0	39.0	31.0	40.0
76-77	34.43924213211582	35.0	33.5	37.0	29.5	39.0
78-79	34.75055799729644	35.0	34.0	37.0	30.0	39.0
80-81	34.59841079507338	35.0	34.0	37.0	30.0	39.0
82-83	34.33902018628922	35.0	34.0	36.0	30.0	38.5
84-85	34.074800324078	35.0	34.0	36.0	30.0	37.0
86-87	33.83232520212554	35.0	34.0	35.5	30.0	37.0
88-89	33.59345693363679	35.0	33.0	35.0	30.0	36.5
90-91	33.47154391204106	35.0	33.0	35.0	29.0	36.0
92-93	33.40047008552415	35.0	33.0	35.0	30.0	36.0
94-95	33.31751587216942	35.0	33.0	35.0	29.0	36.0
96-97	33.31034753733402	35.0	33.0	35.0	30.0	35.0
98-99	33.34505343057903	34.0	33.0	35.0	30.0	35.0
100-101	32.1411271574443	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	0.0
19	18.0
20	77.0
21	377.0
22	1723.0
23	5788.0
24	14576.0
25	29388.0
26	51852.0
27	80916.0
28	114376.0
29	152660.0
30	195070.0
31	243897.0
32	307954.0
33	397085.0
34	546651.0
35	782721.0
36	1287596.0
37	2469110.0
38	3222778.0
39	1465450.0
40	62035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.41068031338777	21.65693281697438	15.445448819153858	24.486938050483992
2	13.570446374681817	21.772858879820856	41.77989170843502	22.876803037062306
3	16.865324776793916	27.262449138543648	31.60440537451072	24.267820710151717
4	11.764143481775767	19.346154323891547	38.844940459685134	30.04476173464755
5	12.561148209509046	38.42671399555286	35.88434630329092	13.127791491647175
6	28.626952794925437	38.879263923717794	19.0028565240469	13.49092675730987
7	25.4170388744088	33.37867182281547	23.24537508918115	17.958914213594586
8	24.13895236306613	36.84445277580986	21.45452169356054	17.56207316756346
9	25.14837671283803	16.85918098678715	21.30464789779611	36.68779440257872
10-11	23.633206017744524	27.109429373394136	29.094175732776083	20.16318887608526
12-13	24.763549504522224	24.642946084169857	29.04157478719783	21.551929624110084
14-15	21.976127271649762	26.02980690525982	26.738151163622202	25.25591465946821
16-17	21.38314507237751	29.34443077521666	27.320279978908335	21.95214417349749
18-19	21.46703424572488	28.167711291150965	29.073699811106422	21.291554652017737
20-21	22.286075610426355	27.170164376063894	29.07550803602268	21.468251977487075
22-23	21.870865468021474	27.55270701256867	28.86118645839758	21.715241061012275
24-25	21.791949012677176	27.76326741846925	29.050140416057168	21.3946431527964
26-27	21.79741395163112	27.86547241881164	29.015196493090034	21.321917136467206
28-29	21.277582543512228	27.54915551017543	29.188795404630348	21.98446654168199
30-31	21.878441226807563	27.373503398057974	29.33686387755607	21.41119149757839
32-33	21.502292466577742	27.91939715705951	29.013917791901832	21.56439258446092
34-35	21.565856861318576	27.9284551499466	29.371515403896648	21.134172584838183
36-37	21.91401190713321	27.63809839984065	28.92947347453688	21.518416218489257
38-39	21.548639994479622	27.5742674645989	29.14884433754893	21.72824820337255
40-41	21.686802710125452	27.493859069550723	29.18567750962734	21.633660710696486
42-43	21.897498716519582	27.437569941331	29.01179501801929	21.653136324130138
44-45	21.729763280238785	27.85276676776175	28.633929815735907	21.783540136263554
46-47	22.12990130241044	27.498181026372986	28.133962084786614	22.237955586429962
48-49	21.947449372873752	27.63724174470511	28.264923668418962	22.15038521400217
50-51	21.731661970556907	27.850215822112922	28.296719254275228	22.12140295305495
52-53	21.829645747369042	27.969394894800214	28.38853268376429	21.812426674066455
54-55	21.85699738865527	27.95650252318036	28.02598921570349	22.160510872460883
56-57	22.14246155413647	28.21064174350088	27.63836797341258	22.008528728950065
58-59	21.7380742285117	28.535711235374716	28.136240296848953	21.589974239264624
60-61	22.128854369666737	28.690852894419304	27.569361361326646	21.610931374587317
62-63	21.789010648953564	28.90516219866327	27.538824875478095	21.767002276905075
64-65	21.838180106802323	28.717730036354077	27.592941990924487	21.851147865919117
66-67	21.984800715046664	28.478033137730762	27.530164435923428	22.007001711299143
68-69	21.990219758846585	28.198471429426185	27.729570917660002	22.081737894067228
70-71	22.020287827120672	27.955295001119524	27.828012942225527	22.19640422953428
72-73	22.077457927926528	27.985753658348088	27.815556012982324	22.12123240074306
74-75	21.930349150626693	28.09226066832221	27.76945709137139	22.207933089679706
76-77	21.99318122185156	28.044999378191626	27.716934037066693	22.24488536289012
78-79	21.989118424372055	28.000987694939898	27.71475606081435	22.295137819873695
80-81	22.02705078157389	28.022682723784193	27.73838307789427	22.21188341674765
82-83	22.05396820724877	27.96921189715431	27.881354125462643	22.09546577013428
84-85	22.217864689254334	27.90513065989905	27.71736334951594	22.159641301330673
86-87	21.984404909360286	27.95269407695366	27.83864313519423	22.224257878491823
88-89	22.099261961175966	27.86235141141164	27.763614688883347	22.27477193852905
90-91	22.098814053797778	27.923728793549785	27.831910519003756	22.145546633648685
92-93	22.172558221396375	27.836943737962834	27.846169756583077	22.144328284057714
94-95	22.00496203166348	27.839136091649102	27.879073531943575	22.27682834474384
96-97	21.90247691763022	27.901470972993035	27.98773215771389	22.20831995166285
98-99	22.158314336027967	28.482562678140262	27.99933087989294	21.35979210593883
100-101	22.638646587685365	31.21882023781495	25.121905699757352	21.020627474742337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1970.0
1	1928.0
2	3104.5
3	4541.5
4	6076.5
5	9060.5
6	11321.5
7	13302.5
8	14909.5
9	15150.5
10	14222.0
11	13186.5
12	14721.0
13	20406.5
14	34384.5
15	53486.0
16	68761.5
17	77066.0
18	77696.5
19	72401.5
20	64589.0
21	56945.0
22	52656.5
23	54608.5
24	63093.0
25	78521.0
26	99474.5
27	122671.5
28	148230.5
29	175220.5
30	201516.0
31	227926.0
32	257140.5
33	287731.5
34	316691.0
35	344016.5
36	371433.5
37	394265.5
38	408384.0
39	419757.0
40	433504.0
41	445422.0
42	451903.0
43	459152.5
44	469000.0
45	483919.5
46	508447.5
47	530023.0
48	522969.5
49	501727.5
50	482382.0
51	454638.5
52	421856.0
53	389222.5
54	359880.0
55	329523.5
56	298940.0
57	267364.5
58	232968.5
59	199886.0
60	167114.5
61	134377.0
62	103437.0
63	78286.5
64	58816.0
65	43639.5
66	32886.5
67	24906.5
68	18677.0
69	13114.0
70	8956.5
71	6480.5
72	4580.0
73	3090.5
74	2034.5
75	1305.0
76	854.5
77	538.0
78	339.0
79	223.0
80	161.5
81	117.5
82	90.5
83	63.5
84	40.5
85	28.5
86	21.5
87	17.0
88	14.5
89	11.5
90	9.0
91	7.0
92	5.5
93	4.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7494597012395184E-5
2	8.747298506197592E-6
3	5.685744029028435E-4
4	1.312094775929639E-4
5	3.586392387541013E-4
6	2.186824626549398E-4
7	0.0027379044324398463
8	5.948162984214363E-4
9	4.461122238160772E-4
10-11	9.184663431507471E-5
12-13	3.498919402479037E-5
14-15	1.6182502236465547E-4
16-17	8.484879551011664E-4
18-19	2.186824626549398E-5
20-21	0.0
22-23	0.0029175717736872383
24-25	0.007118305582514747
26-27	0.036025490693575385
28-29	0.279819843146722
30-31	0.24381320431454442
32-33	0.2512214374689225
34-35	0.1308161665759935
36-37	0.022007859368141213
38-39	0.013879155995971218
40-41	0.007084799175743441
42-43	0.004148679462686871
44-45	0.0051751395226270905
46-47	9.759408933367037E-4
48-49	0.001632127713296075
50-51	0.0022473633946729042
52-53	0.0011778842719714917
54-55	7.15619390289417E-5
56-57	0.0013687574838863747
58-59	0.0025821945408802077
60-61	0.010822761928449671
62-63	0.0063560203243214165
64-65	0.0013658044005814597
66-67	0.0016692583561695022
68-69	0.002704565192277003
70-71	0.004173522514192722
72-73	0.003758299776896542
74-75	0.0038140094550042233
76-77	0.001727965616940154
78-79	7.646382761208218E-5
80-81	4.588042687370274E-4
82-83	1.678703718418267E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	5.017366877598737E-4
92-93	0.00782170823948583
94-95	0.0010863061045825945
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	847.0
22-23	1607.0
24-25	4284.0
26-27	6712.0
28-29	10022.0
30-31	22856.0
32-33	30187.0
34-35	43104.0
36-37	61131.0
38-39	74461.0
40-41	79083.0
42-43	94736.0
44-45	112693.0
46-47	112060.0
48-49	98276.0
50-51	88877.0
52-53	86587.0
54-55	100586.0
56-57	113346.0
58-59	111947.0
60-61	114245.0
62-63	114931.0
64-65	118241.0
66-67	133367.0
68-69	111348.0
70-71	102835.0
72-73	98501.0
74-75	100118.0
76-77	103080.0
78-79	109171.0
80-81	110139.0
82-83	108834.0
84-85	111389.0
86-87	116169.0
88-89	123020.0
90-91	133825.0
92-93	138461.0
94-95	154476.0
96-97	226510.0
98-99	952288.0
100-101	6897551.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.38300985486003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82658714438959	74.06628191234222
2	7.504718970516919	12.515321117531347
3	1.99933898105994	5.001327058827805
4	0.7601059468477716	2.535196866269818
5	0.34839974184089123	1.4525309553674863
6	0.18394811334628824	0.9202888408761878
7	0.11508765886704994	0.6717448775438797
8	0.071188117481674	0.4748703601218685
9	0.047881129109298495	0.35932253943442155
>10	0.1397894744683474	1.7323090414034454
>50	0.0023215534993519834	0.127474092687225
>100	5.617538831450381E-4	0.07903592647432484
>500	3.570772524931914E-5	0.021377850828988562
>1k	3.5706964436269344E-5	0.04291856029107335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.747298506197592E-6	0.0	0.0	0.0
3	0.0	8.747298506197592E-6	0.0	8.747298506197592E-6	0.0
4	0.0	3.498919402479037E-5	0.0	8.747298506197592E-6	0.0
5	0.0	3.498919402479037E-5	0.0	8.747298506197592E-6	0.0
6	0.0	3.498919402479037E-5	0.0	8.747298506197592E-6	0.0
7	0.0	3.498919402479037E-5	0.0	8.747298506197592E-6	0.0
8	0.0	3.498919402479037E-5	0.0	8.747298506197592E-6	0.0
9	0.0	8.747298506197592E-5	0.0	1.7494597012395184E-5	0.0
10-11	8.747298506197592E-6	8.747298506197592E-5	0.0	3.498919402479037E-5	0.0
12-13	8.747298506197592E-6	8.747298506197592E-5	0.0	3.498919402479037E-5	0.0
14-15	8.747298506197592E-6	1.7494597012395185E-4	0.0	3.498919402479037E-5	4.373649253098796E-6
16-17	8.747298506197592E-6	1.9244056713634702E-4	0.0	3.498919402479037E-5	8.747298506197592E-6
18-19	8.747298506197592E-6	2.1430881340184102E-4	0.0	3.498919402479037E-5	8.747298506197592E-6
20-21	8.747298506197592E-6	2.536716566797302E-4	0.0	3.936284327788916E-5	8.747298506197592E-6
22-23	8.747298506197592E-6	3.017817984638169E-4	0.0	5.2483791037185555E-5	2.186824626549398E-5
24-25	8.747298506197592E-6	3.411446417417061E-4	0.0	6.997838804958074E-5	3.936284327788916E-5
26-27	8.747298506197592E-6	3.498919402479037E-4	0.0	1.093412313274699E-4	4.373649253098796E-5
28-29	8.747298506197592E-6	3.498919402479037E-4	0.0	3.280236939824097E-4	4.373649253098796E-5
30-31	8.747298506197592E-6	3.8488113427269404E-4	0.0	9.403345894162412E-4	4.811014178408676E-5
32-33	8.747298506197592E-6	3.9362843277889164E-4	0.0	0.002379265193685745	6.997838804958074E-5
34-35	8.747298506197592E-6	4.242439775505832E-4	0.0	0.004526726976957254	6.997838804958074E-5
36-37	8.747298506197592E-6	4.548595223222748E-4	0.0	0.00777634837200966	6.997838804958074E-5
38-39	8.747298506197592E-6	4.679804700815712E-4	0.0	0.01285852880411046	7.872568655577834E-5
40-41	8.747298506197592E-6	5.073433133594604E-4	0.0	0.018426184303305226	7.872568655577834E-5
42-43	8.747298506197592E-6	5.292115596249544E-4	0.0	0.023910740466691116	7.872568655577834E-5
44-45	1.7494597012395184E-5	5.773217014090411E-4	0.0	0.030331257570240147	7.872568655577834E-5
46-47	1.7494597012395184E-5	6.21058193940029E-4	0.0	0.03746467950204428	7.872568655577834E-5
48-49	1.7494597012395184E-5	6.298054924462267E-4	0.0	0.04449313385177405	7.872568655577834E-5
50-51	1.7494597012395184E-5	6.954102312427086E-4	0.0	0.05135101588063297	7.872568655577834E-5
52-53	1.7494597012395184E-5	7.129048282551037E-4	0.0	0.058077688431898915	9.622028356817352E-5
54-55	1.7494597012395184E-5	7.172784775082026E-4	0.0	0.06497055965478261	1.0496758207437111E-4
56-57	1.7494597012395184E-5	7.347730745205978E-4	0.0	0.07202525590003098	1.0496758207437111E-4
58-59	1.7494597012395184E-5	7.347730745205978E-4	0.0	0.07859885072743847	1.0496758207437111E-4
60-61	1.7494597012395184E-5	7.522676715329929E-4	0.0	0.08456888195791831	1.137148805805687E-4
62-63	1.7494597012395184E-5	7.785095670515857E-4	0.0	0.09018902124815029	1.3120947759296387E-4
64-65	1.7494597012395184E-5	8.572352536073641E-4	0.0	0.09579603959062294	1.3120947759296387E-4
66-67	1.7494597012395184E-5	8.572352536073641E-4	0.0	0.10171796067931871	1.3120947759296387E-4
68-69	1.7494597012395184E-5	8.922244476321544E-4	0.0	0.10781920138739151	1.3558312684606267E-4
70-71	1.7494597012395184E-5	9.140926938976484E-4	0.0	0.11368863868505011	1.4433042535226027E-4
72-73	1.7494597012395184E-5	9.184663431507471E-4	0.0	0.11840343257989061	1.6619867161775425E-4
74-75	1.7494597012395184E-5	9.272136416569448E-4	0.0	0.12237907975095741	1.6619867161775425E-4
76-77	2.6241895518592778E-5	9.66576484934834E-4	0.0	0.1256636903400346	1.7057232087085305E-4
78-79	2.6241895518592778E-5	9.796974326941304E-4	0.0	0.12881709145151884	1.7494597012395185E-4
80-81	2.6241895518592778E-5	9.88444731200328E-4	0.0	0.13021228556325737	1.7931961937705065E-4
82-83	2.6241895518592778E-5	9.88444731200328E-4	0.0	0.13063652954080796	1.8806691788324822E-4
84-85	2.6241895518592778E-5	9.88444731200328E-4	0.0	0.13088145389898148	1.9244056713634702E-4
86-87	2.6241895518592778E-5	9.88444731200328E-4	0.0	0.13099079513030895	1.9681421638944582E-4
88-89	2.6241895518592778E-5	0.0010146866267189208	0.0	0.13102578432433373	2.0118786564254462E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	13045	0.0	45.53529	1
GGTATCA	21405	0.0	39.159157	1
TATACAC	17930	0.0	35.627087	3
TTATACA	18210	0.0	33.68724	2
GTATCAA	35080	0.0	31.481312	1
ATCAACG	42140	0.0	25.635687	3
TCAACGC	42360	0.0	25.62212	4
CAACGCA	42860	0.0	25.29364	5
TATCAAC	43075	0.0	25.2852	2
TACACAT	25345	0.0	25.237158	5
ACACATC	24300	0.0	24.983625	6
ACATCTC	24365	0.0	24.864738	8
AACGCAG	43930	0.0	24.764011	6
CACATCT	26415	0.0	22.8073	7
ATACACA	30775	0.0	22.006062	4
CATCTCC	27380	0.0	21.84897	9
ACGCAGA	50005	0.0	21.730137	7
CGCAGAG	50250	0.0	21.59878	8
GTACATG	40990	0.0	20.469545	1
TACATGG	40500	0.0	20.247868	2
>>END_MODULE