FastQCFastQC Report
Fri 27 May 2016
SRR2139007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11464781
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162600.050.104871
GTATCAA293750.036.3648031
ATCAACG349500.029.9466743
TCAACGC367300.028.5646654
CAACGCA371250.028.2493445
AACGCAG375850.027.9597846
TATCAAC379500.027.8025112
ACGCAGA389000.027.0036147
CTTATAC89550.025.331971
CGCAGAG445900.023.5382678
TATACAC127750.020.6063543
GTACATG365100.020.5757791
TACATGG361900.020.2784182
GCAGAGT515350.020.2182779
ACATGGG354850.020.0133063
GAGTACT302250.019.00813712-13
AGAGTAC471650.018.56533610-11
CAGAGTA503300.018.18828610-11
GTGGTAT85000.017.6758041
CATGGGG271800.017.5021114
Produced by FastQC (version 0.11.5)