##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11464781
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60063127241593	34.0	31.0	34.0	31.0	34.0
2	32.728850904347844	34.0	31.0	34.0	31.0	34.0
3	33.37912795717598	34.0	31.0	34.0	31.0	37.0
4	36.17585412228982	37.0	35.0	37.0	35.0	37.0
5	36.12923631075029	37.0	35.0	37.0	35.0	37.0
6	36.155796695985735	37.0	36.0	37.0	35.0	37.0
7	36.16356474667942	37.0	36.0	37.0	35.0	37.0
8	36.44183294909863	37.0	37.0	37.0	35.0	39.0
9	37.78696767081726	39.0	38.0	39.0	35.0	39.0
10-11	37.748342292800885	39.0	38.0	39.0	35.0	39.0
12-13	37.87980555407033	39.0	38.0	39.0	35.0	40.0
14-15	39.03755972312075	40.0	38.0	41.0	36.0	41.0
16-17	38.992254016888765	40.0	38.0	41.0	35.0	41.0
18-19	38.948829768313935	40.0	38.5	41.0	35.0	41.0
20-21	38.99194108738099	40.0	38.5	41.0	35.0	41.0
22-23	38.922968317438404	40.0	38.0	41.0	35.0	41.0
24-25	38.83571232706278	40.0	38.0	41.0	35.0	41.0
26-27	38.75380425612356	40.0	38.0	41.0	35.0	41.0
28-29	38.53835964186735	40.0	38.0	41.0	34.0	41.0
30-31	38.476030820431376	40.0	38.0	41.0	34.0	41.0
32-33	38.38444067999282	40.0	38.0	41.0	34.0	41.0
34-35	38.22557233530003	40.0	38.0	41.0	34.0	41.0
36-37	38.369611715493726	40.0	38.0	41.0	34.0	41.0
38-39	38.461813014173515	40.0	38.0	41.0	34.0	41.0
40-41	38.448861955407665	40.0	38.0	41.0	34.0	41.0
42-43	38.337130343840016	40.0	38.0	41.0	34.0	41.0
44-45	38.28841159004295	40.0	38.0	41.0	33.0	41.0
46-47	38.28516847382147	40.0	38.0	41.0	33.0	41.0
48-49	38.17834940020957	40.0	38.0	41.0	33.0	41.0
50-51	38.097662698235666	40.0	37.0	41.0	33.0	41.0
52-53	38.027843448074584	40.0	37.0	41.0	33.0	41.0
54-55	37.91209644927379	40.0	37.0	41.0	33.0	41.0
56-57	37.75419848894244	40.0	36.5	41.0	33.0	41.0
58-59	37.60179217778254	39.0	36.0	41.0	33.0	41.0
60-61	37.41381525377018	39.0	36.0	41.0	32.0	41.0
62-63	37.1970676518212	39.0	35.0	40.5	32.0	41.0
64-65	36.958862466940175	38.5	35.0	40.0	32.0	41.0
66-67	36.68413021702109	38.0	35.0	40.0	31.0	41.0
68-69	36.3791024664789	37.0	35.0	40.0	31.0	41.0
70-71	36.079631591228846	37.0	35.0	39.5	31.0	41.0
72-73	35.74983474305132	36.0	34.5	39.0	31.0	40.5
74-75	35.32406324988701	36.0	34.0	38.5	30.5	40.0
76-77	34.51064847296871	35.0	33.5	37.0	29.5	39.0
78-79	34.753624496132105	35.0	34.0	37.0	30.0	39.0
80-81	34.58361316231904	35.0	34.0	37.0	30.0	39.0
82-83	34.32088498787563	35.0	34.0	36.0	30.0	38.5
84-85	34.055720616533854	35.0	34.0	36.0	30.0	37.0
86-87	33.80731565486073	35.0	34.0	35.5	30.0	37.0
88-89	33.58278555748068	35.0	33.0	35.0	29.5	36.5
90-91	33.4688300576676	35.0	33.0	35.0	29.0	36.0
92-93	33.39157466082558	35.0	33.0	35.0	29.5	36.0
94-95	33.31527678124672	35.0	33.0	35.0	29.0	36.0
96-97	33.31023780828112	35.0	33.0	35.0	30.0	35.0
98-99	33.31389801203962	34.0	33.0	35.0	30.0	35.0
100-101	31.88798451585415	33.0	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	7.0
20	80.0
21	439.0
22	1713.0
23	5832.0
24	14445.0
25	29265.0
26	51403.0
27	79597.0
28	113472.0
29	151179.0
30	193265.0
31	242264.0
32	306130.0
33	396285.0
34	547932.0
35	780032.0
36	1282739.0
37	2449572.0
38	3216916.0
39	1515001.0
40	87208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66174690327655	0.02106451419604338	26.593604639042756	30.723583943484652
2	14.029476933710175	25.194009713765514	38.041796074074284	22.734717278450027
3	15.509685970689574	25.318459420661256	34.004214317899965	25.167640290749212
4	12.597020205672152	24.533354453140763	37.43540526959547	25.43422007159161
5	16.86982881591496	36.8585176071246	32.55750198827116	13.71415158868928
6	27.15999869512572	38.08383747476822	20.641785319222315	14.114378510883752
7	25.35570724334388	33.38206935436431	22.968892450807207	18.293330951484602
8	23.859016058844926	32.452273105904986	22.371896018303122	21.31681481694696
9	23.451964593007375	20.79622208648237	24.15964739194719	31.59216592856306
10-11	24.340657550656577	26.372023278783196	28.908545039416172	20.378774131144052
12-13	24.649999795024396	24.486808685976417	28.82359093009992	22.039600588899273
14-15	22.04914694689231	26.855003780715535	26.92779204032252	24.168057232069643
16-17	21.172416695577994	29.21739232828906	27.840368471700298	21.769822504432646
18-19	21.321495624641564	28.27720125340445	29.576869149198302	20.824433972755678
20-21	22.07690837582232	27.487257664918086	29.46189764210649	20.973936317153107
22-23	21.86310568065931	27.326290049751677	29.21811002946018	21.592494240128833
24-25	21.65716518287251	27.580018068032985	29.44272108905278	21.320095660041723
26-27	21.580942035582	27.82773255997821	29.239036938039277	21.352288466400516
28-29	21.403426706339847	27.682559371255866	29.22899873836834	21.685015184035947
30-31	21.763736131237245	27.583440571214357	29.409520093127057	21.243303204421345
32-33	21.538166828030036	27.875877810215492	29.142097223997776	21.443858137756695
34-35	21.64451614792078	27.727782727019978	29.2467150643119	21.380986060747343
36-37	21.738691988844742	27.77400465139649	29.1894948914314	21.29780846832737
38-39	21.574658500360634	27.87297419085	29.152001838023473	21.400365470765895
40-41	21.78306755970116	27.624631546288636	28.94107954196042	21.651221352049784
42-43	21.761382607521348	27.719086717553882	28.912207497540898	21.607323177383876
44-45	21.773528749986067	28.098113896078754	28.53316704065078	21.5951903132844
46-47	21.845782254717363	27.771467178478034	28.306278975810883	22.076471590993723
48-49	21.80490839500138	28.003331597391185	28.332813912697212	21.858946094910223
50-51	21.83582916788381	28.234023345504433	28.017120798567124	21.913026688044635
52-53	21.905019452318193	28.037974537079123	28.093004074839982	21.9640019357627
54-55	21.836420418217948	28.0506919300819	28.14456058302387	21.968327068676277
56-57	21.936078352169677	28.402320249805022	27.752842947192512	21.90875845083278
58-59	21.88800130751293	28.296930440642143	28.011751505381987	21.803316746462936
60-61	22.051536155002474	28.350878716475187	27.828864077130362	21.768721051391978
62-63	21.87690199572112	28.55429125862312	27.75714289390152	21.811663851754233
64-65	21.933554816611768	28.24851634983086	27.783385476436873	22.0345433571205
66-67	21.910818092601815	28.301544912264383	27.788114428720718	21.999522566413088
68-69	21.972487604613995	28.28115952389463	27.816349143939668	21.930003727551714
70-71	22.011315234607718	27.876235886085038	27.89537864653716	22.217070232770084
72-73	21.976265592900745	28.057511224781724	27.84942162219047	22.116801560127065
74-75	21.87585645063933	28.224455474578715	27.81780131583489	22.08188675894707
76-77	22.03214354016073	27.985519062429752	27.729583077900628	22.25275431950889
78-79	21.838231943936847	28.067918007824034	27.879690119269164	22.214159928969956
80-81	21.959022744083747	28.16364593256694	27.765483603179717	22.111847720169596
82-83	22.033716017976523	27.89996185581498	27.901467547328902	22.164854578879595
84-85	22.075345586965692	28.02529796570901	27.831826317760083	22.06753012956521
86-87	21.937560428027457	28.114507599840298	27.791335314820483	22.15659665731176
88-89	22.028964911411105	27.89128946775034	27.865187983690443	22.214557637148115
90-91	22.036900390460325	27.974287184586267	27.887036891668338	22.10177553328507
92-93	22.055819159980565	27.975822231673586	27.858341787873016	22.110016820472833
94-95	22.013258280685836	27.876905131851686	27.923879647876237	22.18595693958624
96-97	21.78536038004857	28.023771894685222	28.102783841141637	22.088083884124575
98-99	22.22823627458163	28.96335143004553	27.942988861923073	20.86542343344977
100-101	22.583443559941184	31.0468524985359	24.825147015550467	21.54455692597245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2300.0
1	2127.5
2	3232.5
3	4799.0
4	6454.5
5	9603.0
6	11964.0
7	14345.5
8	16414.5
9	16687.0
10	15488.5
11	13961.0
12	15110.5
13	19833.5
14	32536.0
15	52233.0
16	69391.5
17	78527.5
18	78755.5
19	73230.5
20	65625.0
21	58549.0
22	54874.0
23	57503.5
24	66685.5
25	81619.0
26	102341.5
27	126970.5
28	153584.5
29	183451.5
30	212697.0
31	239526.5
32	268376.0
33	297532.5
34	326063.5
35	352047.5
36	378078.5
37	400567.0
38	415084.5
39	425947.0
40	435433.0
41	445080.0
42	451797.5
43	457268.0
44	464093.0
45	469956.5
46	476910.5
47	480335.5
48	476669.5
49	468308.0
50	456645.0
51	439113.0
52	415070.5
53	388386.5
54	362566.5
55	336644.0
56	307449.5
57	274250.5
58	239436.5
59	208190.0
60	176123.5
61	142561.0
62	112202.5
63	85941.5
64	63372.0
65	47374.5
66	36440.0
67	27844.5
68	21083.0
69	14843.0
70	10314.5
71	7591.0
72	5387.0
73	3661.5
74	2447.5
75	1589.5
76	1071.5
77	693.5
78	462.0
79	313.0
80	208.0
81	147.5
82	106.5
83	70.5
84	48.5
85	34.0
86	22.5
87	15.5
88	11.5
89	10.5
90	10.0
91	8.5
92	6.5
93	4.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.744472921026577E-5
2	1.744472921026577E-4
3	4.797300532823087E-4
4	2.6167093815398655E-4
5	4.0995113644124554E-4
6	6.541773453849664E-4
7	0.002590542287724467
8	6.192878869644348E-4
9	3.488945842053154E-4
10-11	5.669536993336375E-5
12-13	1.744472921026577E-5
14-15	1.744472921026577E-4
16-17	0.0011208238517595756
18-19	5.233418763079731E-5
20-21	1.8753765199981212E-4
22-23	0.003245138268188346
24-25	0.010981512011535955
26-27	0.07190865043517523
28-29	0.2737372738047581
30-31	0.24665908500658634
32-33	0.24559551838921814
34-35	0.10816891220361792
36-37	0.02380855395529046
38-39	0.015126373225101457
40-41	0.008771671510926683
42-43	0.006014660893734423
44-45	0.006692817337590893
46-47	0.0014021654675700046
48-49	0.0018711793755730487
50-51	0.0027543016758520443
52-53	0.001426251577082404
54-55	2.0002412671966575E-4
56-57	0.0016678127472129862
58-59	0.0034889734125183106
60-61	0.012886984380974931
62-63	0.008060100941755256
64-65	0.0021134598161589744
66-67	0.0019846379929401425
68-69	0.0025632643774366965
70-71	0.004180368771489224
72-73	0.003926655089031038
74-75	0.003372335624326027
76-77	0.0012887968458348137
78-79	1.4541189129976419E-4
80-81	4.522879531032975E-4
82-83	2.7576760049481534E-5
84-85	1.1164937896986812E-5
86-87	1.1306845164062323E-5
88-89	5.730289594510382E-5
90-91	0.0013838908591566593
92-93	0.007341988649982534
94-95	9.1351857847374E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	242.0
20-21	865.0
22-23	1861.0
24-25	5465.0
26-27	6852.0
28-29	12085.0
30-31	24530.0
32-33	33054.0
34-35	47288.0
36-37	64919.0
38-39	76053.0
40-41	79740.0
42-43	95213.0
44-45	112286.0
46-47	110526.0
48-49	97549.0
50-51	87625.0
52-53	87091.0
54-55	93463.0
56-57	99417.0
58-59	98869.0
60-61	99456.0
62-63	98140.0
64-65	102580.0
66-67	110864.0
68-69	105074.0
70-71	100739.0
72-73	98987.0
74-75	100276.0
76-77	102937.0
78-79	107447.0
80-81	110170.0
82-83	109260.0
84-85	111216.0
86-87	116266.0
88-89	124835.0
90-91	132514.0
92-93	140792.0
94-95	163265.0
96-97	292147.0
98-99	1139148.0
100-101	6763675.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.5196582121971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.87841047027676	70.75939575297848
2	7.641378539556206	12.305623765501672
3	2.258656208575323	5.455986778000258
4	0.958462228785679	3.087002042844941
5	0.4457592022117424	1.7946189303515574
6	0.25609148516238384	1.2372239313797455
7	0.14638831912740896	0.8250996195677901
8	0.10490073432744644	0.6757257019403583
9	0.06655692374077844	0.4823226676136471
>10	0.23814292412522303	2.9500318044056133
>50	0.00417622069624593	0.21614322333485375
>100	0.0010117988329517694	0.17094362116171474
>500	4.32966318005734E-5	0.021527470149427838
>1k	2.164795010196227E-5	0.018354690769945482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.722364605132885E-6	8.722364605132885E-6
2	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	8.722364605132885E-6
3	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	8.722364605132885E-6
4	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	8.722364605132885E-6
5	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	8.722364605132885E-6
6	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	1.744472921026577E-5
7	0.0	1.3083546907699328E-4	0.0	8.722364605132885E-6	1.744472921026577E-5
8	0.0	1.4828019828725905E-4	0.0	2.6167093815398655E-5	1.744472921026577E-5
9	0.0	2.6167093815398655E-4	0.0	3.488945842053154E-5	1.744472921026577E-5
10-11	0.0	2.6167093815398655E-4	0.0	3.488945842053154E-5	1.744472921026577E-5
12-13	0.0	2.6167093815398655E-4	0.0	3.488945842053154E-5	1.744472921026577E-5
14-15	0.0	3.7506167802071406E-4	0.0	3.925064072309798E-5	2.1805911512832213E-5
16-17	0.0	4.2739586565151137E-4	0.0	4.3611823025664425E-5	2.6167093815398655E-5
18-19	0.0	4.2739586565151137E-4	0.0	5.669536993336375E-5	2.6167093815398655E-5
20-21	0.0	4.2739586565151137E-4	0.0	6.10565522359302E-5	2.6167093815398655E-5
22-23	0.0	5.233418763079731E-4	0.0	6.977891684106308E-5	3.488945842053154E-5
24-25	0.0	5.538701524259381E-4	0.0	6.977891684106308E-5	4.3611823025664425E-5
26-27	0.0	5.669536993336375E-4	0.0	1.1775192216929395E-4	4.3611823025664425E-5
28-29	0.0	5.71314881636204E-4	0.0	3.7506167802071406E-4	4.3611823025664425E-5
30-31	0.0	7.021503507131972E-4	0.0	0.001273465232349401	4.797300532823087E-5
32-33	0.0	7.065115330157636E-4	0.0	0.002808601402852789	5.233418763079731E-5
34-35	0.0	8.547917313030226E-4	0.0	0.0047013545221666255	5.233418763079731E-5
36-37	0.0	9.943495649851488E-4	0.0	0.008216467458035177	5.233418763079731E-5
38-39	0.0	0.0010641284818262119	0.0	0.01312715873072499	5.233418763079731E-5
40-41	0.0	0.001142629763272408	0.0	0.018526302421302245	6.10565522359302E-5
42-43	0.0	0.0012865487792571005	0.0	0.02406064276325906	6.10565522359302E-5
44-45	0.0	0.0014304677952417931	0.0	0.030092157887708453	6.10565522359302E-5
46-47	0.0	0.0014304677952417931	0.0	0.03717471794707636	6.10565522359302E-5
48-49	0.0	0.0014304677952417931	0.0	0.043943272880659474	6.977891684106308E-5
50-51	0.0	0.0016528880926726817	0.0	0.050437073329180904	6.977891684106308E-5
52-53	0.0	0.001665971639580381	0.0	0.05695704087151774	7.414009914362952E-5
54-55	0.0	0.0016703328218829476	0.0	0.0640439621131882	7.850128144619597E-5
56-57	0.0	0.0017008610980009126	0.0	0.07082123941137647	7.850128144619597E-5
58-59	0.0	0.001713944644908612	0.0	0.07663469542069752	8.722364605132885E-5
60-61	0.0	0.001744472921026577	0.0	0.08303691104086507	9.594601065646174E-5
62-63	0.0	0.0017880847440522414	0.0	0.0888547282324887	9.594601065646174E-5
64-65	0.0	0.0020061438591805637	0.0	0.09464637833029693	9.594601065646174E-5
66-67	0.0	0.002041033317601095	0.0	0.10047727906882828	9.594601065646174E-5
68-69	0.0	0.0020715615937190604	0.0	0.10669632503228801	9.594601065646174E-5
70-71	0.0	0.0020933675052318924	0.0	0.11233097256720387	9.594601065646174E-5
72-73	0.0	0.0021151734167447244	0.0	0.11660057004141641	1.221131044718604E-4
74-75	0.0	0.0021544240574678224	0.0	0.12026396317557221	1.221131044718604E-4
76-77	0.0	0.002228564156611452	0.0	0.12356537817861502	1.221131044718604E-4
78-79	0.0	0.0022503700681242842	0.0	0.12641323022219092	1.221131044718604E-4
80-81	0.0	0.0022590924327294173	0.0	0.1275427764385556	1.221131044718604E-4
82-83	0.0	0.002276537161939683	0.0	0.1280225064918379	1.221131044718604E-4
84-85	0.0	0.0022983430734525153	0.0	0.12835395634683297	1.221131044718604E-4
86-87	0.0	0.0023288713495704804	0.0	0.12844990235748943	1.221131044718604E-4
88-89	0.0	0.002503318641673138	0.0	0.12847170826900225	1.221131044718604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16260	0.0	50.10487	1
GTATCAA	29375	0.0	36.364803	1
ATCAACG	34950	0.0	29.946674	3
TCAACGC	36730	0.0	28.564665	4
CAACGCA	37125	0.0	28.249344	5
AACGCAG	37585	0.0	27.959784	6
TATCAAC	37950	0.0	27.802511	2
ACGCAGA	38900	0.0	27.003614	7
CTTATAC	8955	0.0	25.33197	1
CGCAGAG	44590	0.0	23.538267	8
TATACAC	12775	0.0	20.606354	3
GTACATG	36510	0.0	20.575779	1
TACATGG	36190	0.0	20.278418	2
GCAGAGT	51535	0.0	20.218277	9
ACATGGG	35485	0.0	20.013306	3
GAGTACT	30225	0.0	19.008137	12-13
AGAGTAC	47165	0.0	18.565336	10-11
CAGAGTA	50330	0.0	18.188286	10-11
GTGGTAT	8500	0.0	17.675804	1
CATGGGG	27180	0.0	17.502111	4
>>END_MODULE