FastQCFastQC Report
Fri 27 May 2016
SRR2139006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10706359
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145400.045.6246031
GTATCAA258950.035.4293441
ATCAACG315100.028.586683
TCAACGC315450.028.5549644
CAACGCA322700.027.9796985
TATCAAC329400.027.6441842
AACGCAG333950.027.062746
ACGCAGA383850.023.5333677
CGCAGAG387300.023.300788
GTACATG309550.020.2422371
GCAGAGT452900.019.7935859
TACATGG310800.019.5828362
ACATGGG315600.019.0411453
AGAGTAC401750.018.8218310-11
GAGTACT264900.018.7935412-13
CAGAGTA438300.017.65235110-11
ACCGAAC14400.017.2266568
CATGGGG233850.017.1195454
AGTACTT293050.017.04663312-13
GTACTTT297950.016.65215114-15
Produced by FastQC (version 0.11.5)