##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10706359
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48478245498773	34.0	31.0	34.0	31.0	34.0
2	32.6287609074196	34.0	31.0	34.0	31.0	34.0
3	32.62417830375387	34.0	31.0	34.0	31.0	34.0
4	36.188386453321804	37.0	35.0	37.0	35.0	37.0
5	36.12329681827408	37.0	35.0	37.0	35.0	37.0
6	36.1474932794613	37.0	36.0	37.0	35.0	37.0
7	36.09694556291266	37.0	36.0	37.0	35.0	37.0
8	36.083996155929384	37.0	36.0	37.0	35.0	37.0
9	37.78870641270296	39.0	38.0	39.0	35.0	39.0
10-11	37.728671904239334	39.0	37.5	39.0	35.0	39.0
12-13	37.69959483891769	39.0	37.0	39.0	35.0	39.0
14-15	39.03663304210143	40.0	38.0	41.0	36.0	41.0
16-17	39.01298546032316	40.0	38.0	41.0	36.0	41.0
18-19	38.932629197283596	40.0	38.5	41.0	35.0	41.0
20-21	38.99046596732508	40.0	38.0	41.0	35.0	41.0
22-23	38.920933807941765	40.0	38.0	41.0	35.0	41.0
24-25	38.84742040373492	40.0	38.0	41.0	35.0	41.0
26-27	38.76621675779322	40.0	38.0	41.0	35.0	41.0
28-29	38.55081071243602	40.0	38.0	41.0	34.5	41.0
30-31	38.48346209800456	40.0	38.0	41.0	34.0	41.0
32-33	38.416601652552586	40.0	38.0	41.0	34.0	41.0
34-35	38.22929420347129	40.0	38.0	41.0	34.0	41.0
36-37	38.36184620850883	40.0	38.0	41.0	34.0	41.0
38-39	38.48301677774995	40.0	38.0	41.0	34.0	41.0
40-41	38.48021895144133	40.0	38.0	41.0	34.0	41.0
42-43	38.353038513118214	40.0	38.0	41.0	34.0	41.0
44-45	38.291645766408266	40.0	38.0	41.0	33.0	41.0
46-47	38.306111313129904	40.0	38.0	41.0	33.0	41.0
48-49	38.18076340880406	40.0	38.0	41.0	33.0	41.0
50-51	38.1235199439499	40.0	37.5	41.0	33.0	41.0
52-53	38.04918300813989	40.0	37.0	41.0	33.0	41.0
54-55	37.941020634496525	40.0	37.0	41.0	33.0	41.0
56-57	37.789477207542376	40.0	36.5	41.0	33.0	41.0
58-59	37.63511499960757	39.5	36.0	41.0	33.0	41.0
60-61	37.45718980474564	39.0	36.0	41.0	32.5	41.0
62-63	37.24158742163083	39.0	35.0	40.5	32.0	41.0
64-65	36.999816959976215	38.5	35.0	40.0	32.0	41.0
66-67	36.72312465528782	38.0	35.0	40.0	31.5	41.0
68-69	36.41133105299481	37.0	35.0	40.0	31.0	41.0
70-71	36.115524214364065	37.0	35.0	39.5	31.0	41.0
72-73	35.78842523658508	36.5	34.5	39.0	31.0	40.5
74-75	35.462895778083855	36.0	34.0	39.0	31.0	40.0
76-77	34.442695401525555	35.0	33.5	37.0	29.5	39.0
78-79	34.75620083646973	35.0	34.0	37.0	30.0	39.0
80-81	34.603246972836885	35.0	34.0	37.0	30.0	39.0
82-83	34.340602637320046	35.0	34.0	36.0	30.0	38.5
84-85	34.074580032552255	35.0	34.0	36.0	30.0	37.0
86-87	33.826543072153385	35.0	34.0	35.5	30.0	37.0
88-89	33.584637433764556	35.0	33.0	35.0	29.0	36.5
90-91	33.462185507388114	35.0	33.0	35.0	29.0	36.0
92-93	33.39084058244582	35.0	33.0	35.0	29.0	36.0
94-95	33.30578348511384	35.0	33.0	35.0	29.0	36.0
96-97	33.29443015822131	35.0	33.0	35.0	29.5	35.0
98-99	33.32994402033273	34.0	33.0	35.0	30.0	35.0
100-101	32.13098438125578	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	12.0
20	59.0
21	329.0
22	1509.0
23	5201.0
24	13072.0
25	27091.0
26	47686.0
27	73990.0
28	106328.0
29	141702.0
30	182299.0
31	228951.0
32	288115.0
33	373370.0
34	517025.0
35	737402.0
36	1213511.0
37	2318809.0
38	3025141.0
39	1350412.0
40	54343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.76914644838642	21.532166070650163	15.399492955541655	24.29919452542176
2	13.714485008395478	22.00604332434584	41.34425157983214	22.935220087426547
3	16.915582553589505	27.004731982887826	31.302101362058615	24.777584101464054
4	11.956876770504508	19.00688573248321	39.28680209233014	29.74943540468214
5	12.760178392028326	38.27010207440324	35.93364771497327	13.036071818595168
6	29.15188063889277	38.940160194875276	18.94344796795542	12.964511198276535
7	25.756110573877088	33.129607840335105	23.755404993035263	17.35887659275254
8	24.085314595961407	37.13913783879105	21.32250307856007	17.453044486687467
9	25.3733987838019	16.882307311152342	21.57588301857362	36.16841088647214
10-11	23.73613497416719	27.144884645506938	29.323256442791635	19.795723937534238
12-13	24.730100733157983	24.782060524704228	29.37770467270585	21.110134069431936
14-15	21.806874503565364	26.156034216722347	27.13146056207422	24.905630717638065
16-17	21.261021644012406	29.221651419283674	27.732850027462895	21.784476909241025
18-19	21.32572632662416	28.456741706971766	29.520114567442757	20.697417398961317
20-21	22.133801370101324	27.532626224938518	29.408000460302787	20.92557194465737
22-23	21.838569676481516	27.444380620334847	29.25512531006046	21.461924393123173
24-25	21.645221925932347	27.588597008088506	29.30436471535388	21.46181635062526
26-27	21.558613006594534	27.747391388328328	29.239899600461982	21.45409600461515
28-29	21.504699812560926	27.797033946100186	29.196933906680055	21.50133233465883
30-31	21.79448595815632	27.69223302820966	29.137519115796266	21.37576189783775
32-33	21.59397590253765	27.823729830249217	29.064047171534472	21.51824709567866
34-35	21.736727420065346	27.848740762727235	29.096406856959785	21.31812496024763
36-37	21.735666069831552	27.854740416416174	28.985929840144486	21.423663673607788
38-39	21.57978888706922	27.945973607581642	29.039033889881154	21.435203615467984
40-41	21.75454817374993	27.83973269594068	28.91052290754173	21.495196222767664
42-43	21.722114840811706	27.94024395017274	28.620730810592626	21.716910398422932
44-45	21.85339894757206	28.00500089735293	28.46266545672933	21.678934698345685
46-47	21.810190977874182	28.078258175100313	28.23563645563265	21.875914391392858
48-49	21.845167437585925	28.208860171598715	28.12490520115517	21.82106718966019
50-51	21.883313744039505	28.27278017638648	28.002269277556756	21.84163680201726
52-53	21.96314205715757	28.19216758657312	27.90884880278064	21.93584155348867
54-55	21.951848439767776	28.11989429754707	27.9448756700795	21.983381592605653
56-57	21.939080239077104	28.192222261800502	27.934559150415968	21.934138348706426
58-59	21.86718867516219	28.29504202244682	27.956842747621284	21.880926554769704
60-61	22.00733720733721	28.234301834301835	27.94957554957555	21.808785408785408
62-63	22.000977430111885	28.313766288688214	27.850527976406585	21.834728304793313
64-65	21.943889561871917	28.25543702484405	27.847536837158227	21.953136576125807
66-67	22.03163239145179	28.239710426682542	27.715554868227915	22.01310231363775
68-69	21.941310433398748	28.24159835066632	27.771133597667326	22.045957618267604
70-71	21.887424865307654	28.097668019581317	27.875412028958035	22.139495086152994
72-73	21.979457645717194	28.11863119108942	27.86450730953402	22.037403853659367
74-75	21.894647274614954	28.23277528740077	27.78039630327903	22.09218113470525
76-77	21.912967798866877	28.156417017903212	27.754813522551864	22.175801660678047
78-79	21.908093369748467	28.16953018587615	27.74544148471722	22.17693495965816
80-81	21.93136406711768	28.181569540474648	27.751758005657955	22.135308386749713
82-83	21.9555415942725	28.17298308312725	27.81605908966011	22.05541623294014
84-85	22.035174387828636	28.17828797122417	27.74835099322077	22.03818664772642
86-87	21.900432062786027	28.206733818326345	27.783585179790034	22.109248939097593
88-89	21.93939133449358	28.157192187091695	27.7343243731492	22.169092105265534
90-91	21.95953073655773	28.23577395591191	27.76867585292122	22.03601945460914
92-93	21.99035040108387	28.16001385433512	27.788192685012636	22.061443059568376
94-95	21.82928963909973	28.254067082286905	27.831571524233684	22.085071754379683
96-97	21.784416188390164	28.335613173443086	27.847440925741097	22.032529712425656
98-99	22.01143437250747	28.85880669607631	27.89902827346268	21.230730657953544
100-101	22.4757078471478	31.614498019881125	25.089747775894534	20.820046357076542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3520.0
1	2994.5
2	3547.5
3	4863.0
4	6194.0
5	8826.5
6	11019.5
7	13226.0
8	15170.0
9	15718.5
10	14863.5
11	13565.0
12	14514.0
13	18870.0
14	30105.5
15	46276.5
16	59218.0
17	66146.0
18	67326.0
19	64044.0
20	58972.0
21	54153.5
22	51848.5
23	55029.5
24	63726.0
25	77700.5
26	96665.5
27	118013.0
28	142498.0
29	170484.0
30	197834.0
31	224930.0
32	252455.5
33	278540.0
34	302835.0
35	324917.0
36	348791.5
37	371926.5
38	388367.0
39	399599.0
40	410228.0
41	420864.5
42	427507.5
43	431868.5
44	436258.5
45	439515.5
46	440094.5
47	437934.5
48	433318.0
49	425063.0
50	413751.0
51	397283.0
52	376144.5
53	353598.0
54	331054.0
55	306112.5
56	279433.0
57	250959.5
58	220369.5
59	188952.5
60	155961.0
61	126255.0
62	100145.0
63	76946.5
64	57865.0
65	44335.0
66	34817.0
67	26908.5
68	20563.5
69	14493.5
70	9757.5
71	6962.0
72	4951.0
73	3456.0
74	2325.0
75	1511.0
76	989.5
77	671.5
78	443.0
79	295.5
80	198.5
81	131.0
82	97.0
83	72.0
84	50.5
85	35.5
86	27.0
87	18.5
88	11.5
89	11.0
90	9.5
91	8.0
92	6.5
93	3.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.071158271453442E-4
4	1.681243829017876E-4
5	4.763524182217316E-4
6	2.2416584386905015E-4
7	0.0026713096394395145
8	5.137133921999066E-4
9	4.1097071375992527E-4
10-11	1.307634089236126E-4
12-13	3.269085223090315E-5
14-15	1.4010365241815635E-4
16-17	8.919932537289287E-4
18-19	1.4010365241815634E-5
20-21	0.0
22-23	0.0028631284035555665
24-25	0.006909820719352049
26-27	0.03509319866419618
28-29	0.2789175235046102
30-31	0.2436543194200017
32-33	0.25109014686659786
34-35	0.12983735182619244
36-37	0.02113117998436491
38-39	0.012455814991528194
40-41	0.005899664962138398
42-43	0.003090794396264587
44-45	0.0042777819724750706
46-47	6.520818349955455E-4
48-49	0.0012223179622148361
50-51	0.0019011749310973785
52-53	8.096049927183322E-4
54-55	4.5612061410255E-5
56-57	0.0010783119175135332
58-59	0.002098011142140255
60-61	0.009546298142121917
62-63	0.005112917873533785
64-65	8.4640146334349E-4
66-67	0.0014738403746502233
68-69	0.002453535034404223
70-71	0.0040683821514683185
72-73	0.0036249446357090164
74-75	0.0037498693795499456
76-77	0.001537869877349826
78-79	7.942473346335918E-5
80-81	3.7293454505313224E-4
82-83	1.741290543556032E-5
84-85	0.0
86-87	5.942764639480697E-6
88-89	0.0
90-91	4.5161889199943095E-4
92-93	0.008138016297213732
94-95	0.0011518181985261259
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	712.0
22-23	1637.0
24-25	5172.0
26-27	6063.0
28-29	9053.0
30-31	21196.0
32-33	27339.0
34-35	38321.0
36-37	54861.0
38-39	66494.0
40-41	70442.0
42-43	83444.0
44-45	98367.0
46-47	96969.0
48-49	86146.0
50-51	77927.0
52-53	76394.0
54-55	80757.0
56-57	84257.0
58-59	83507.0
60-61	82082.0
62-63	81758.0
64-65	87357.0
66-67	90791.0
68-69	91346.0
70-71	91772.0
72-73	89235.0
74-75	91397.0
76-77	93663.0
78-79	97943.0
80-81	100700.0
82-83	99214.0
84-85	100263.0
86-87	105982.0
88-89	111833.0
90-91	118183.0
92-93	125728.0
94-95	143133.0
96-97	219424.0
98-99	917493.0
100-101	6697778.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.9278947900279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17199532059695	74.8401784148392
2	7.293440259481669	12.242461735102626
3	1.9229073032617092	4.84156685517375
4	0.7464545704554215	2.5059344261887233
5	0.33023538904723637	1.3857980493950195
6	0.18236968040095	0.9183542009749168
7	0.10650125080602334	0.6256898040858012
8	0.06297387750237449	0.4228211972431512
9	0.043113336482056716	0.32565704114818145
>10	0.13746579775261655	1.7146905225602562
>50	0.0021929629634466056	0.1195036364130703
>100	3.316731290602268E-4	0.04875280065783155
>500	1.857812051535628E-5	0.008591316217491138
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8680486989087515E-5	0.0	9.340243494543757E-6	0.0
3	0.0	1.8680486989087515E-5	0.0	1.8680486989087515E-5	0.0
4	0.0	5.604146096726254E-5	0.0	3.736097397817503E-5	0.0
5	0.0	5.604146096726254E-5	0.0	3.736097397817503E-5	0.0
6	9.340243494543757E-6	5.604146096726254E-5	0.0	3.736097397817503E-5	9.340243494543757E-6
7	9.340243494543757E-6	7.472194795635006E-5	0.0	3.736097397817503E-5	9.340243494543757E-6
8	9.340243494543757E-6	7.472194795635006E-5	0.0	3.736097397817503E-5	9.340243494543757E-6
9	9.340243494543757E-6	1.7746462639633138E-4	0.0	5.604146096726254E-5	9.340243494543757E-6
10-11	9.340243494543757E-6	1.7746462639633138E-4	0.0	7.472194795635006E-5	9.340243494543757E-6
12-13	9.340243494543757E-6	1.8213474814360325E-4	0.0	8.873231319816568E-5	9.340243494543757E-6
14-15	1.4010365241815636E-5	2.3350608736359392E-4	0.0	1.307634089236126E-4	1.4010365241815636E-5
16-17	1.8680486989087515E-5	2.3350608736359392E-4	0.0	1.307634089236126E-4	1.8680486989087515E-5
18-19	1.8680486989087515E-5	2.4284633085813767E-4	0.0	1.5878413940724386E-4	1.8680486989087515E-5
20-21	1.8680486989087515E-5	2.568566960999533E-4	0.0	1.8680486989087515E-4	2.3350608736359394E-5
22-23	1.8680486989087515E-5	3.0355791357267214E-4	0.0	2.148256003745064E-4	3.736097397817502E-5
24-25	1.8680486989087515E-5	3.222384005617596E-4	0.0	2.4751645260540957E-4	4.670121747271878E-5
26-27	1.8680486989087515E-5	3.362487658035752E-4	0.0	3.0822803531994393E-4	4.670121747271878E-5
28-29	1.8680486989087515E-5	3.362487658035752E-4	0.0	4.8102253996900343E-4	4.670121747271878E-5
30-31	1.8680486989087515E-5	3.7360973978175024E-4	0.0	0.0011068188541034352	4.670121747271878E-5
32-33	1.8680486989087515E-5	3.7360973978175024E-4	0.0	0.00253587610876863	6.0711582714534416E-5
34-35	1.8680486989087515E-5	4.2965120074901276E-4	0.0	0.004520677851359178	6.53817044618063E-5
36-37	1.8680486989087515E-5	4.8569266171627533E-4	0.0	0.007738391735229503	6.53817044618063E-5
38-39	1.8680486989087515E-5	5.137133921999066E-4	0.0	0.012875525657228569	6.53817044618063E-5
40-41	1.8680486989087515E-5	6.071158271453442E-4	0.0	0.018367588832020297	6.53817044618063E-5
42-43	1.8680486989087515E-5	6.257963141344317E-4	0.0	0.023724218476141142	6.53817044618063E-5
44-45	1.8680486989087515E-5	6.91178018596238E-4	0.0	0.029561870660230992	6.53817044618063E-5
46-47	1.8680486989087515E-5	7.378792360689568E-4	0.0	0.03645030023745701	7.005182620907819E-5
48-49	1.8680486989087515E-5	7.378792360689568E-4	0.0	0.04300248104887945	7.472194795635006E-5
50-51	1.8680486989087515E-5	8.359517927616661E-4	0.0	0.04935851674691648	7.472194795635006E-5
52-53	1.8680486989087515E-5	8.499621580034818E-4	0.0	0.055901357314844385	7.939206970362193E-5
54-55	1.8680486989087515E-5	8.546322797507537E-4	0.0	0.06296258139671947	8.40621914508938E-5
56-57	1.8680486989087515E-5	8.593024014980256E-4	0.0	0.0700985274265509	8.40621914508938E-5
58-59	1.8680486989087515E-5	8.686426449925693E-4	0.0	0.0761089741152898	8.40621914508938E-5
60-61	1.8680486989087515E-5	8.966633754762006E-4	0.0	0.08228754518693049	8.40621914508938E-5
62-63	1.8680486989087515E-5	9.386944712016475E-4	0.0	0.08774224738774405	8.40621914508938E-5
64-65	1.8680486989087515E-5	0.001074128001872532	0.0	0.09358923981532843	8.40621914508938E-5
66-67	1.8680486989087515E-5	0.0010881383671143478	0.0	0.09942689199941829	8.40621914508938E-5
68-69	1.8680486989087515E-5	0.0011021487323561634	0.0	0.10547469966213538	8.40621914508938E-5
70-71	1.8680486989087515E-5	0.0011208292193452508	9.340243494543757E-6	0.11139174391592885	8.40621914508938E-5
72-73	1.8680486989087515E-5	0.0011254993410925226	9.340243494543757E-6	0.1158190193323426	1.307634089236126E-4
74-75	1.8680486989087515E-5	0.001195551167301601	9.340243494543757E-6	0.11957846733889643	1.307634089236126E-4
76-77	1.8680486989087515E-5	0.001265602993510679	9.340243494543757E-6	0.12266074769209588	1.307634089236126E-4
78-79	1.8680486989087515E-5	0.001298293845741582	9.340243494543757E-6	0.12566830609733898	1.307634089236126E-4
80-81	1.8680486989087515E-5	0.001298293845741582	9.340243494543757E-6	0.12707401274326782	1.3543353067088448E-4
82-83	1.8680486989087515E-5	0.001298293845741582	9.340243494543757E-6	0.12754569503974228	1.4010365241815635E-4
84-85	1.8680486989087515E-5	0.0013169743327306697	9.340243494543757E-6	0.1278772736837986	1.4010365241815635E-4
86-87	1.8680486989087515E-5	0.0013543353067088447	9.340243494543757E-6	0.12801270721446945	1.4010365241815635E-4
88-89	1.8680486989087515E-5	0.001419717011170651	9.340243494543757E-6	0.12807341879718398	1.4010365241815635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14540	0.0	45.624603	1
GTATCAA	25895	0.0	35.429344	1
ATCAACG	31510	0.0	28.58668	3
TCAACGC	31545	0.0	28.554964	4
CAACGCA	32270	0.0	27.979698	5
TATCAAC	32940	0.0	27.644184	2
AACGCAG	33395	0.0	27.06274	6
ACGCAGA	38385	0.0	23.533367	7
CGCAGAG	38730	0.0	23.30078	8
GTACATG	30955	0.0	20.242237	1
GCAGAGT	45290	0.0	19.793585	9
TACATGG	31080	0.0	19.582836	2
ACATGGG	31560	0.0	19.041145	3
AGAGTAC	40175	0.0	18.82183	10-11
GAGTACT	26490	0.0	18.79354	12-13
CAGAGTA	43830	0.0	17.652351	10-11
ACCGAAC	1440	0.0	17.226656	8
CATGGGG	23385	0.0	17.119545	4
AGTACTT	29305	0.0	17.046633	12-13
GTACTTT	29795	0.0	16.652151	14-15
>>END_MODULE