FastQCFastQC Report
Fri 27 May 2016
SRR2139005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519333
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130700.049.955481
GTATCAA235850.037.5442961
ATCAACG272550.031.8827253
TCAACGC273500.031.740194
CAACGCA277450.031.3196495
AACGCAG287800.030.2983256
TATCAAC292050.029.9623152
ACGCAGA328350.026.5298677
CGCAGAG333200.026.0913948
GCAGAGT397550.021.857149
GAGTACT232150.020.01618412-13
GTACATG288150.019.2344761
CAGAGTA392900.019.15081810-11
TACATGG284250.018.8713722
TGGTATC61750.018.5143132
GTGGTAT67050.018.4771461
ACATGGG284200.018.3852353
AGAGTAC361000.018.38069310-11
GTACTTT269550.017.3197814-15
AGTACTT255450.016.33569512-13
Produced by FastQC (version 0.11.5)