##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10519333
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18154753728207	33.0	33.0	34.0	30.0	34.0
2	31.264249453838946	33.0	32.0	33.0	27.0	34.0
3	32.50105705371244	33.0	33.0	33.0	31.0	34.0
4	32.92072586731497	33.0	33.0	34.0	32.0	34.0
5	33.15594049546677	34.0	33.0	34.0	33.0	34.0
6	37.079363967278155	38.0	37.0	38.0	36.0	38.0
7	37.14108993412415	38.0	38.0	38.0	36.0	38.0
8	37.349403331941296	38.0	38.0	38.0	37.0	38.0
9	37.33426415914393	38.0	38.0	38.0	37.0	38.0
10-11	37.39181604955371	38.0	38.0	38.0	37.0	38.0
12-13	37.39903414028247	38.0	38.0	38.0	37.0	38.0
14-15	37.406015523988074	38.0	38.0	38.0	37.0	38.0
16-17	37.36747729157352	38.0	38.0	38.0	37.0	38.0
18-19	37.348742928853	38.0	38.0	38.0	37.0	38.0
20-21	37.40624515240851	38.0	38.0	38.0	37.0	38.0
22-23	37.42238122482155	38.0	38.0	38.0	37.0	38.0
24-25	37.42550329226849	38.0	38.0	38.0	37.0	38.0
26-27	37.32676567946277	38.0	38.0	38.0	37.0	38.0
28-29	37.228730516836656	38.0	38.0	38.0	37.0	38.0
30-31	37.31404205568268	38.0	38.0	38.0	37.0	38.0
32-33	37.33496725054077	38.0	38.0	38.0	37.0	38.0
34-35	37.2920100082136	38.0	38.0	38.0	37.0	38.0
36-37	37.2956119389306	38.0	38.0	38.0	37.0	38.0
38-39	37.29367384397467	38.0	38.0	38.0	37.0	38.0
40-41	37.254107569284386	38.0	38.0	38.0	37.0	38.0
42-43	37.25361958715031	38.0	38.0	38.0	37.0	38.0
44-45	37.18948998946128	38.0	38.0	38.0	37.0	38.0
46-47	37.16817606554095	38.0	38.0	38.0	37.0	38.0
48-49	37.14787313487501	38.0	38.0	38.0	37.0	38.0
50-51	37.14973898015183	38.0	38.0	38.0	37.0	38.0
52-53	37.13784188236984	38.0	38.0	38.0	36.5	38.0
54-55	37.093668920097755	38.0	38.0	38.0	36.0	38.0
56-57	37.10058343363713	38.0	38.0	38.0	36.0	38.0
58-59	37.11812863922923	38.0	38.0	38.0	36.0	38.0
60-61	37.1344855750972	38.0	38.0	38.0	36.0	38.0
62-63	37.06992270475851	38.0	38.0	38.0	36.0	38.0
64-65	37.10570374620081	38.0	38.0	38.0	36.0	38.0
66-67	37.13364718928423	38.0	38.0	38.0	36.0	38.0
68-69	37.109515768728166	38.0	38.0	38.0	36.0	38.0
70-71	37.067031727173664	38.0	38.0	38.0	36.0	38.0
72-73	37.08473092118125	38.0	38.0	38.0	36.0	38.0
74-75	37.11050786567503	38.0	38.0	38.0	36.0	38.0
76-77	37.05665437355866	38.0	38.0	38.0	36.0	38.0
78-79	37.086864334648965	38.0	38.0	38.0	36.0	38.0
80-81	37.06895021094756	38.0	38.0	38.0	36.0	38.0
82-83	37.020517923125894	38.0	38.0	38.0	36.0	38.0
84-85	37.056381233561	38.0	38.0	38.0	36.0	38.0
86-87	37.08541770491953	38.0	38.0	38.0	36.0	38.0
88-89	37.001208291401305	38.0	38.0	38.0	36.0	38.0
90-91	36.967618323126345	38.0	38.0	38.0	36.0	38.0
92-93	37.026834031879105	38.0	38.0	38.0	36.0	38.0
94-95	37.01638099599232	38.0	38.0	38.0	36.0	38.0
96-97	36.96999825570077	38.0	38.0	38.0	36.0	38.0
98-99	36.98385243934611	38.0	38.0	38.0	36.0	38.0
100	35.524268306075726	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	9.0
19	30.0
20	70.0
21	230.0
22	670.0
23	1905.0
24	4320.0
25	8821.0
26	15487.0
27	25014.0
28	39240.0
29	57396.0
30	78985.0
31	104514.0
32	136228.0
33	181095.0
34	265336.0
35	478262.0
36	1350081.0
37	7771637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77067433838248	21.003556023941822	14.399059331993769	23.826710305681928
2	14.424647750553483	21.17662347218038	42.35428313291323	22.0444456443529
3	17.515207475607056	26.93517735392539	30.82643167584865	24.723183494618908
4	12.110621514986509	18.605019255982892	40.117163377736674	29.167195851293926
5	12.94546259320126	37.82424189590739	36.24665451789931	12.98364099299204
6	28.77409170166112	38.831266684830126	19.514851103012123	12.879790510496624
7	25.624700372828173	33.171992151966435	24.01875101658158	17.184556458623813
8	23.645253933875846	37.110185598269396	21.841812594011426	17.40274787384333
9	25.301290490566274	17.182990594555758	21.93572539247498	35.57999352240299
10-11	23.466649453914997	27.15575217554193	29.67128714339588	19.706311227147197
12-13	24.561975554913985	24.790350300727244	29.632629749433733	21.015044394925038
14-15	21.734372321895314	26.120719821304263	27.372861948566513	24.772045908233917
16-17	21.198059800939852	29.200539615962345	28.009161797615878	21.592238785481932
18-19	21.149216399937142	28.421583383661304	29.769311419269645	20.65988879713191
20-21	22.019367464729747	27.533387751082195	29.638677777038918	20.80856700714914
22-23	21.766970614374344	27.452831762655077	29.411440921090225	21.36875670188035
24-25	21.527898564175807	27.55237259926091	29.542175567919752	21.37755326864353
26-27	21.523995588532905	27.73576112663458	29.366191749544896	21.374051535287624
28-29	21.424809104242033	27.7859373113552	29.419637238026386	21.36961634637638
30-31	21.7296766317101	27.6324691967152	29.365626084159857	21.27222808741485
32-33	21.546228040529915	27.702470874627515	29.340085078739865	21.411216006102705
34-35	21.6354733016726	27.745215306150072	29.336778092948446	21.28253329922888
36-37	21.623108029465033	27.67943931986916	29.3235242978198	21.373928352846008
38-39	21.49498499791868	27.81870915969828	29.366776780524734	21.319529061858304
40-41	21.77547538286004	27.667961973237592	29.20184280090891	21.354719842993454
42-43	21.625241573848307	27.72843468404193	29.077779917814496	21.568543824295265
44-45	21.74251003867191	27.82128063069881	28.95782792575427	21.47838140487501
46-47	21.738126337227836	27.81169587597938	28.76917180310966	21.681005983683118
48-49	21.71033090117539	27.932256291468725	28.766017919368792	21.591394887987093
50-51	21.6864138614559	28.026721961007667	28.627431157437677	21.659433020098756
52-53	21.77211242995722	27.996736357976342	28.509750758169144	21.721400453897292
54-55	21.818248237289254	27.924845778599046	28.526179389759616	21.73072659435209
56-57	21.839368846935734	27.978318891504006	28.488267215506035	21.69404504605422
58-59	21.768178093671786	28.04942337719717	28.542862040562223	21.63953648856883
60-61	21.867445223298027	27.995601735634533	28.553758447638867	21.583194593428576
62-63	21.865181705454372	27.99216378229106	28.48053240643446	21.66212210582011
64-65	21.828770385922965	27.980885306396836	28.47895439396641	21.711389913713788
66-67	21.866375724499772	27.989552020058774	28.37920821414299	21.764864041298466
68-69	21.845145896580462	27.957840036512604	28.41037378391619	21.78664028299075
70-71	21.852113326046936	27.801402414732344	28.47651652518401	21.86996773403671
72-73	21.9082717822487	27.86112457736524	28.40724889584365	21.823354744542414
74-75	21.83990500947283	27.96468083842285	28.352021644469094	21.843392507635226
76-77	21.925231939070073	27.889528316518653	28.28799997894006	21.897239765471216
78-79	21.832536082560885	27.904569696389192	28.295548671819553	21.96734554923037
80-81	21.859730338091413	27.92614718311446	28.306897991156436	21.907224487637688
82-83	21.950027923646264	27.944836510538757	28.289843173062255	21.81529239275272
84-85	22.042335492019472	27.90433733357095	28.22215640301005	21.831170771399528
86-87	21.876163417766996	27.93135519877617	28.291094196250878	21.901387187205955
88-89	21.946128387172536	27.898780896313717	28.21566187436651	21.939428842147237
90-91	21.97527982521305	27.991967422425944	28.185764246806666	21.84698850555434
92-93	22.02422921419216	27.94821648184672	28.19591133626854	21.83164296769258
94-95	21.93324549080042	28.035001943846886	28.18534667623546	21.84640588911723
96-97	21.998849592668403	28.14268806950791	28.218838148788024	21.639624189035665
98-99	23.06329736457786	29.9976941524602	28.505941240410987	18.433067242550955
100	21.93892615916814	30.94435196965824	22.445716251979817	24.6710056191938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1410.0
1	1394.5
2	2140.0
3	3257.0
4	4400.0
5	6087.0
6	7418.0
7	9190.5
8	11537.5
9	12950.5
10	13185.0
11	13482.0
12	15050.5
13	18464.0
14	24680.0
15	31549.0
16	36492.0
17	38994.0
18	38422.5
19	35598.5
20	32727.5
21	31129.0
22	32723.5
23	38944.5
24	49752.5
25	65337.5
26	85062.5
27	108407.0
28	134103.0
29	161932.5
30	189135.0
31	215823.5
32	244586.5
33	272214.5
34	299506.0
35	324824.5
36	348580.5
37	370734.0
38	386382.0
39	398936.0
40	412139.0
41	423125.0
42	428800.0
43	433685.5
44	438659.5
45	442422.5
46	442655.5
47	437506.5
48	429600.5
49	417708.5
50	403245.0
51	385361.5
52	361507.5
53	335329.5
54	310289.0
55	283119.5
56	255798.5
57	227608.0
58	198205.5
59	169974.0
60	140055.5
61	112095.5
62	87636.5
63	66156.5
64	49085.5
65	36168.5
66	26224.0
67	19283.0
68	14260.0
69	10217.0
70	7269.5
71	5182.0
72	3632.0
73	2432.0
74	1622.5
75	1040.0
76	623.0
77	380.5
78	238.5
79	135.5
80	72.5
81	48.5
82	34.5
83	25.5
84	24.5
85	20.0
86	13.5
87	11.5
88	8.5
89	5.5
90	3.5
91	2.5
92	1.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.1407567380935656E-4
3	0.0
4	4.7531530753898564E-5
5	0.002813866620630795
6	4.4679638908664643E-4
7	9.506306150779713E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0011221973537159876
30-31	0.0
32-33	0.0
34-35	4.774750204168319E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.857586257616452E-6
44-45	4.88719809211606E-6
46-47	1.476613833095582E-5
48-49	7.931374574630468E-5
50-51	4.1915686596411314E-4
52-53	1.907971696545336E-4
54-55	2.7284123217121494E-4
56-57	4.1714222576103526E-4
58-59	3.6374037195885514E-4
60-61	5.1593727667977434E-5
62-63	3.3770416034900114E-4
64-65	1.098772937473698E-4
66-67	1.4234284835525738E-4
68-69	4.5167617560149453E-4
70-71	1.2320758410176475E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	9.694338096850246E-4
90-91	0.0
92-93	5.877624219620486E-6
94-95	7.121400109990025E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	53.0
20-21	213.0
22-23	475.0
24-25	698.0
26-27	1852.0
28-29	4771.0
30-31	14580.0
32-33	19052.0
34-35	26520.0
36-37	42329.0
38-39	50769.0
40-41	50928.0
42-43	59187.0
44-45	70965.0
46-47	73003.0
48-49	67893.0
50-51	62858.0
52-53	61291.0
54-55	65985.0
56-57	68854.0
58-59	68747.0
60-61	67731.0
62-63	67071.0
64-65	71023.0
66-67	74137.0
68-69	75628.0
70-71	74066.0
72-73	71023.0
74-75	72332.0
76-77	74877.0
78-79	76803.0
80-81	76583.0
82-83	73492.0
84-85	72987.0
86-87	76081.0
88-89	79041.0
90-91	78953.0
92-93	79465.0
94-95	95322.0
96-97	317332.0
98-99	722460.0
100-101	7311903.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.25636756417207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49113649817485	73.67450586463367
2	7.734602964474678	12.879098947464772
3	2.071629046876329	5.174289281500533
4	0.8004674937319856	2.6657606352528713
5	0.3612758290868411	1.503925660925252
6	0.1876150382455866	0.9372087950844453
7	0.11186942433701041	0.6519689338256133
8	0.06635007358350063	0.4419252891342233
9	0.04235775425349884	0.31738974815899546
>10	0.1306358323156965	1.5682755616793886
>50	0.0015601817756196361	0.08557150335243303
>100	4.4290741028350686E-4	0.061765194789955236
>500	5.695573418359226E-5	0.038314584197865695
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.506306150779712E-6	0.0	0.0	0.0
3	0.0	9.506306150779712E-6	0.0	0.0	0.0
4	0.0	3.802522460311885E-5	0.0	0.0	0.0
5	0.0	3.802522460311885E-5	0.0	0.0	0.0
6	6.654414305545799E-5	3.802522460311885E-5	0.0	0.0	0.0
7	6.654414305545799E-5	4.753153075389856E-5	0.0	0.0	0.0
8	6.654414305545799E-5	5.703783690467827E-5	0.0	0.0	0.0
9	6.654414305545799E-5	5.703783690467827E-5	0.0	0.0	0.0
10-11	6.654414305545799E-5	5.703783690467827E-5	0.0	0.0	0.0
12-13	7.129729613084785E-5	5.703783690467827E-5	0.0	4.753153075389856E-6	0.0
14-15	7.60504492062377E-5	7.129729613084785E-5	0.0	9.506306150779712E-6	0.0
16-17	9.506306150779711E-5	8.55567553570174E-5	0.0	9.506306150779712E-6	0.0
18-19	1.0456936765857684E-4	9.030990843240727E-5	0.0	1.4259459226169568E-5	0.0
20-21	1.0456936765857684E-4	1.0456936765857684E-4	0.0	3.802522460311885E-5	0.0
22-23	1.0456936765857684E-4	1.0456936765857684E-4	0.0	5.228468382928842E-5	9.506306150779712E-6
24-25	1.0932252073396668E-4	1.0456936765857684E-4	0.0	8.080360228162754E-5	9.506306150779712E-6
26-27	1.1407567380935654E-4	1.0456936765857684E-4	0.0	1.3308828611091598E-4	9.506306150779712E-6
28-29	1.1407567380935654E-4	1.0456936765857684E-4	0.0	3.279675622019001E-4	9.506306150779712E-6
30-31	1.1407567380935654E-4	1.1407567380935654E-4	0.0	0.0010314342173595988	9.506306150779712E-6
32-33	1.188288268847464E-4	1.1407567380935654E-4	0.0	0.0025476900484089626	9.506306150779712E-6
34-35	1.3308828611091598E-4	1.3308828611091598E-4	0.0	0.0042873440740016505	9.506306150779712E-6
36-37	1.3308828611091598E-4	1.521008984124754E-4	0.0	0.007191520603064851	9.506306150779712E-6
38-39	1.3308828611091598E-4	1.521008984124754E-4	0.0	0.011764053861589893	9.506306150779712E-6
40-41	1.3308828611091598E-4	1.8061981686481453E-4	0.0	0.016464922253150462	9.506306150779712E-6
42-43	1.8061981686481453E-4	1.9012612301559423E-4	0.0	0.020994677133996994	9.506306150779712E-6
44-45	1.9012612301559423E-4	2.2339819454332324E-4	0.0	0.02610431669004109	9.506306150779712E-6
46-47	1.9963242916637395E-4	2.376576537694928E-4	0.0	0.03188890398279055	9.506306150779712E-6
48-49	1.9963242916637395E-4	2.376576537694928E-4	0.0	0.038072756133872745	9.506306150779712E-6
50-51	1.9963242916637395E-4	2.6617657222183195E-4	0.0	0.044337411887236576	9.506306150779712E-6
52-53	1.9963242916637395E-4	2.6617657222183195E-4	0.0	0.050121999179986026	9.506306150779712E-6
54-55	2.0913873531715367E-4	2.709297252972218E-4	0.0	0.05535046756291487	9.506306150779712E-6
56-57	2.1864504146793337E-4	2.8518918452339134E-4	0.0	0.06002757018909849	9.506306150779712E-6
58-59	2.281513476187131E-4	2.8994233759878124E-4	0.0	0.06464763497837743	9.506306150779712E-6
60-61	2.376576537694928E-4	2.946954906741711E-4	0.0	0.06938652859454111	9.506306150779712E-6
62-63	2.376576537694928E-4	2.946954906741711E-4	0.0	0.07406363122072473	9.506306150779712E-6
64-65	2.376576537694928E-4	3.897585521819682E-4	0.0	0.07857912664234509	9.506306150779712E-6
66-67	2.6617657222183195E-4	3.897585521819682E-4	0.0	0.08339407070771503	9.506306150779712E-6
68-69	2.6617657222183195E-4	3.9451170525735805E-4	0.0	0.08825654630383885	9.506306150779712E-6
70-71	2.6617657222183195E-4	3.992648583327479E-4	0.0	0.09237753002020185	9.506306150779712E-6
72-73	2.709297252972218E-4	3.992648583327479E-4	0.0	0.09573800924450249	9.506306150779712E-6
74-75	2.7568287837261165E-4	4.0877116448352765E-4	0.0	0.09822390830293137	9.506306150779712E-6
76-77	2.7568287837261165E-4	4.3729008293586673E-4	0.0	0.10081912988209424	9.506306150779712E-6
78-79	2.7568287837261165E-4	4.467963890866465E-4	0.0	0.10253026498923459	9.506306150779712E-6
80-81	2.7568287837261165E-4	4.467963890866465E-4	0.0	0.10305786498060286	9.506306150779712E-6
82-83	2.8518918452339134E-4	4.467963890866465E-4	0.0	0.10359497127812191	9.506306150779712E-6
84-85	2.946954906741711E-4	4.5154954216203633E-4	0.0	0.1037375658703836	9.506306150779712E-6
86-87	2.946954906741711E-4	4.658090013882059E-4	0.0	0.10383738208496679	9.506306150779712E-6
88	2.946954906741711E-4	4.753153075389856E-4	0.0	0.10384688839111757	9.506306150779712E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13070	0.0	49.95548	1
GTATCAA	23585	0.0	37.544296	1
ATCAACG	27255	0.0	31.882725	3
TCAACGC	27350	0.0	31.74019	4
CAACGCA	27745	0.0	31.319649	5
AACGCAG	28780	0.0	30.298325	6
TATCAAC	29205	0.0	29.962315	2
ACGCAGA	32835	0.0	26.529867	7
CGCAGAG	33320	0.0	26.091394	8
GCAGAGT	39755	0.0	21.85714	9
GAGTACT	23215	0.0	20.016184	12-13
GTACATG	28815	0.0	19.234476	1
CAGAGTA	39290	0.0	19.150818	10-11
TACATGG	28425	0.0	18.871372	2
TGGTATC	6175	0.0	18.514313	2
GTGGTAT	6705	0.0	18.477146	1
ACATGGG	28420	0.0	18.385235	3
AGAGTAC	36100	0.0	18.380693	10-11
GTACTTT	26955	0.0	17.31978	14-15
AGTACTT	25545	0.0	16.335695	12-13
>>END_MODULE