FastQCFastQC Report
Fri 27 May 2016
SRR2139004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9632908
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124000.049.4369661
GTATCAA236750.037.5431441
TCAACGC290700.030.1366464
ATCAACG291000.029.9735723
CAACGCA297900.029.3653355
TATCAAC304250.028.8360732
AACGCAG306700.028.5779256
ACGCAGA353550.024.8150927
CGCAGAG355600.024.6114548
GCAGAGT411650.021.197919
GTACATG281600.020.9970571
TACATGG283550.020.522222
ACATGGG285400.019.7172283
GAGTACT253900.019.23269812-13
AGAGTAC381050.019.051810-11
TGGTATC64800.018.8811422
GTGGTAT68900.018.7472671
GGACCGA16150.018.213696
CAGAGTA408100.018.09191910-11
ACCGAAC14050.017.901358
Produced by FastQC (version 0.11.5)