##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139004_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9632908 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50697816277286 34.0 31.0 34.0 31.0 34.0 2 32.64488272907828 34.0 31.0 34.0 31.0 34.0 3 32.6389771396135 34.0 31.0 34.0 31.0 34.0 4 36.197244383523646 37.0 35.0 37.0 35.0 37.0 5 36.133778709399074 37.0 35.0 37.0 35.0 37.0 6 36.16202853800742 37.0 36.0 37.0 35.0 37.0 7 36.112969520730395 37.0 36.0 37.0 35.0 37.0 8 36.10118491736867 37.0 36.0 37.0 35.0 37.0 9 37.809268706812105 39.0 38.0 39.0 35.0 39.0 10-11 37.75199270043895 39.0 38.0 39.0 35.0 39.0 12-13 37.7225291158184 39.0 38.0 39.0 35.0 39.0 14-15 39.066410423519045 40.0 38.0 41.0 36.0 41.0 16-17 39.04501958287155 40.0 38.0 41.0 36.0 41.0 18-19 38.970377948175155 40.0 38.5 41.0 35.0 41.0 20-21 39.03065535871012 40.0 39.0 41.0 35.0 41.0 22-23 38.961655782793315 40.0 38.0 41.0 35.0 41.0 24-25 38.89048265543238 40.0 38.0 41.0 35.0 41.0 26-27 38.81105154758976 40.0 38.0 41.0 35.0 41.0 28-29 38.59874353985702 40.0 38.0 41.0 34.5 41.0 30-31 38.530304215309755 40.0 38.0 41.0 34.0 41.0 32-33 38.464884145390776 40.0 38.0 41.0 34.0 41.0 34-35 38.280319848196015 40.0 38.0 41.0 34.0 41.0 36-37 38.39960086255271 40.0 38.0 41.0 34.0 41.0 38-39 38.52063928258879 40.0 38.0 41.0 34.0 41.0 40-41 38.51765307497476 40.0 38.0 41.0 34.0 41.0 42-43 38.391054947755784 40.0 38.0 41.0 34.0 41.0 44-45 38.333624421474426 40.0 38.0 41.0 34.0 41.0 46-47 38.352576844986565 40.0 38.0 41.0 34.0 41.0 48-49 38.23093934982221 40.0 38.0 41.0 33.0 41.0 50-51 38.17807492299815 40.0 38.0 41.0 33.0 41.0 52-53 38.11183831173939 40.0 37.0 41.0 33.0 41.0 54-55 38.01129934788145 40.0 37.0 41.0 33.0 41.0 56-57 37.867745535857544 40.0 37.0 41.0 33.0 41.0 58-59 37.72147086728894 40.0 36.5 41.0 33.0 41.0 60-61 37.55196661887986 39.0 36.0 41.0 33.0 41.0 62-63 37.33947310038752 39.0 35.5 41.0 32.0 41.0 64-65 37.105337770333634 39.0 35.0 40.0 32.0 41.0 66-67 36.83610702591423 38.0 35.0 40.0 32.0 41.0 68-69 36.52808035936465 37.5 35.0 40.0 31.0 41.0 70-71 36.235943914528775 37.0 35.0 39.5 31.0 41.0 72-73 35.9082918990942 36.5 35.0 39.0 31.0 41.0 74-75 35.577384339223045 36.0 34.0 39.0 31.0 40.0 76-77 34.55300137155922 35.0 33.5 37.0 29.5 39.0 78-79 34.85965213319599 35.0 34.0 37.0 30.0 39.0 80-81 34.695855816426246 35.0 34.0 37.0 30.0 39.0 82-83 34.42737822900275 35.0 34.0 36.5 30.0 38.5 84-85 34.15388470735424 35.0 34.0 36.0 30.0 37.0 86-87 33.902379527922704 35.0 34.0 36.0 30.0 37.0 88-89 33.65238160960496 35.0 33.5 35.0 30.0 36.5 90-91 33.526067630918334 35.0 33.0 35.0 30.0 36.0 92-93 33.448289090528434 35.0 33.0 35.0 30.0 36.0 94-95 33.35641719806187 35.0 33.0 35.0 30.0 36.0 96-97 33.34249610614282 35.0 33.0 35.0 30.0 35.0 98-99 33.37580008593049 34.5 33.0 35.0 30.0 35.0 100-101 32.15938753787253 33.0 31.0 34.5 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 10.0 20 59.0 21 320.0 22 1412.0 23 4615.0 24 11903.0 25 23970.0 26 41752.0 27 64817.0 28 92605.0 29 123598.0 30 159048.0 31 199533.0 32 252590.0 33 326583.0 34 449217.0 35 637728.0 36 1055369.0 37 2051239.0 38 2790637.0 39 1290009.0 40 55893.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.62430742616871 21.726606337359396 15.496421226072126 24.15266501039977 2 13.673731753692655 22.1372196225688 41.50401934701338 22.68502927672516 3 16.87643830656246 27.115738053179662 31.43956475628827 24.568258883969616 4 11.841966347568771 19.149880850856206 39.65622967574097 29.351923125834055 5 12.574707823014142 38.4114375169991 36.09969417182436 12.914160488162393 6 28.73441795184825 39.26599314016161 19.118269925505142 12.881318982484988 7 25.52083468507099 33.33082450823827 23.871602276545957 17.27673853014478 8 23.872322313749304 37.21768739262005 21.54355149306799 17.36643880056266 9 25.22684299618162 17.002122108106054 21.822625314106673 35.94840958160566 10-11 23.49094462444683 27.346586390366262 29.50060090957061 19.661868075616297 12-13 24.476001787622735 25.01324885898906 29.55948906378709 20.951260289601112 14-15 21.602058862489063 26.355906408999495 27.40824354721303 24.63379118129841 16-17 20.95331580093121 29.435894806002516 28.064963456744955 21.545825936321318 18-19 21.053103644979462 28.6024702303017 29.876332768688997 20.468093356029836 20-21 21.827304059791537 27.65679942815027 29.863493248785726 20.65240326327247 22-23 21.511184352330876 27.591374212842624 29.673471150679458 21.223970284147047 24-25 21.32109125366153 27.735034370739942 29.77411273336819 21.169761642230334 26-27 21.28601959960137 27.900225049090473 29.622351577983686 21.191403773324467 28-29 21.218029440482987 27.93880285433491 29.59804179809817 21.245125907083935 30-31 21.506666791874434 27.887379787501555 29.541358733815592 21.064594686808423 32-33 21.2856564390642 27.962682964681452 29.487803470069945 21.263857126184398 34-35 21.42198161756637 28.024744016353953 29.494560336063376 21.058714030016297 36-37 21.43497822406361 27.987226101048396 29.40194082180627 21.17585485308172 38-39 21.300450574961424 28.120001350631064 29.429225743345118 21.150322331062394 40-41 21.510450165987333 28.011930070357018 29.290762731235393 21.18685703242026 42-43 21.437103970604777 28.12299072829237 29.03236795449311 21.407537346609747 44-45 21.575734337046537 28.19945471294618 28.82721675787206 21.397594192135227 46-47 21.531320535230446 28.265629700984114 28.581388458651425 21.621661305134015 48-49 21.57298638344713 28.4158363346099 28.46803005255858 21.54314722938439 50-51 21.587970607923427 28.481376023953743 28.26363037928355 21.66702298883928 52-53 21.655668614154617 28.40950506494593 28.247933729643837 21.686892591255617 54-55 21.68266958046628 28.333523737766846 28.272895502526808 21.71091117924007 56-57 21.685706093493742 28.410479265895017 28.211176730786825 21.692637909824416 58-59 21.61538773610851 28.4955115636037 28.251348070947508 21.63775262934028 60-61 21.761369526332256 28.4695715313735 28.201246697901755 21.567812244392492 62-63 21.71323081040166 28.49848056596411 28.1516567278532 21.63663189578103 64-65 21.66932226352189 28.415130200153815 28.17514125360376 21.740406282720535 66-67 21.755900005795926 28.45152327129945 28.04953735939145 21.743039363513173 68-69 21.682603976611453 28.390607081958958 28.14254271928749 21.7842462221421 70-71 21.684834690579788 28.255090266991946 28.206706784789322 21.85336825763894 72-73 21.767287432274237 28.27117170365772 28.124705752139146 21.83683511192889 74-75 21.615773296896133 28.356739945862973 28.098180127834677 21.92930662940622 76-77 21.715489486464744 28.272812050313867 28.080049219457585 21.931649243763804 78-79 21.704262634604266 28.28664292157787 28.0755846231182 21.933509820699665 80-81 21.680983604959223 28.33278005062665 28.076606736828385 21.90962960758574 82-83 21.709151125579737 28.285085424325572 28.16537700816969 21.840386441925 84-85 21.82740626658602 28.25739528438617 28.08402220294614 21.831176246081668 86-87 21.697313846984425 28.23609943696069 28.14314737400945 21.923439342045437 88-89 21.756546309769778 28.169915095829317 28.122866300749568 21.950672293651337 90-91 21.77189002418327 28.237745140428338 28.168812185519883 21.821552649868508 92-93 21.810245942410837 28.15103365596054 28.165910898576428 21.872809503052196 94-95 21.714108394139483 28.152940320264747 28.20176689078512 21.931184394810646 96-97 21.625844117429033 28.209870101456026 28.281537767305352 21.882748013809593 98-99 21.847689153027904 28.759862531097934 28.311971380014533 21.080476935859625 100-101 22.279381993188156 31.499281530571754 25.596179540290866 20.62515693594922 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2090.0 1 1942.5 2 2794.0 3 4024.5 4 5382.0 5 7961.0 6 10076.0 7 12266.5 8 14331.0 9 15064.0 10 14365.5 11 13114.5 12 13974.5 13 18348.5 14 29439.0 15 44930.5 16 57251.5 17 63634.5 18 64477.5 19 61128.5 20 55661.5 21 50529.0 22 48076.0 23 51031.5 24 59871.5 25 74083.0 26 92279.0 27 112989.5 28 136379.0 29 161722.5 30 186370.5 31 210605.5 32 236842.0 33 263058.5 34 287264.5 35 310131.5 36 333542.5 37 351840.0 38 363916.5 39 374093.0 40 384238.5 41 394063.5 42 397521.0 43 399777.5 44 401854.5 45 401092.0 46 398029.5 47 391613.5 48 384217.5 49 372992.0 50 358976.0 51 342153.0 52 321316.5 53 299480.5 54 278271.5 55 256201.0 56 232924.5 57 208615.5 58 182794.5 59 156808.0 60 131316.5 61 107274.0 62 84133.5 63 63581.5 64 47595.5 65 36350.5 66 28274.5 67 21880.5 68 16704.5 69 11538.5 70 7616.5 71 5493.0 72 3856.5 73 2631.5 74 1747.0 75 1168.5 76 805.0 77 524.0 78 336.0 79 226.0 80 154.0 81 98.5 82 69.0 83 49.5 84 37.5 85 27.0 86 16.0 87 11.5 88 9.0 89 6.0 90 3.5 91 2.5 92 2.0 93 1.5 94 1.5 95 2.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 5.398162216435577E-4 4 2.3876486726541975E-4 5 4.982918969017456E-4 6 2.906702731926849E-4 7 0.002543364890435993 8 6.021027087562759E-4 9 3.529567603054031E-4 10-11 1.1419189303998336E-4 12-13 3.11432435563591E-5 14-15 1.660972989672485E-4 16-17 7.993432512798835E-4 18-19 5.190540592726516E-6 20-21 0.0 22-23 0.0027928433855449694 24-25 0.0072125757411324 26-27 0.03579422714445085 28-29 0.27863098408772763 30-31 0.24346703046725873 32-33 0.250452810628404 34-35 0.13220039533525946 36-37 0.024342222867285875 38-39 0.015379821357378335 40-41 0.008582889440897606 42-43 0.005532723326930978 44-45 0.006527110417959841 46-47 0.0015119337146426524 48-49 0.0021935210223086596 50-51 0.0026681759648707953 52-53 0.001675186211794409 54-55 2.2023993729373682E-4 56-57 0.0016859351204321555 58-59 0.00303399418093707 60-61 0.012509940417205934 62-63 0.007648779730172337 64-65 0.0021415010695411472 66-67 0.001620212146527936 68-69 0.0026595238994926206 70-71 0.004152368202586932 72-73 0.0037650024291328884 74-75 0.003884420736378202 76-77 0.0017683775343060538 78-79 1.014367051126572E-4 80-81 3.5916213630664853E-4 82-83 6.4903713070030645E-6 84-85 0.0 86-87 1.995107199104915E-5 88-89 0.0 90-91 5.195237361850265E-4 92-93 0.008163420006501582 94-95 0.0011929437588429604 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 172.0 20-21 691.0 22-23 1453.0 24-25 4887.0 26-27 5441.0 28-29 8510.0 30-31 19826.0 32-33 25544.0 34-35 36074.0 36-37 51146.0 38-39 61194.0 40-41 64774.0 42-43 77107.0 44-45 92368.0 46-47 90919.0 48-49 79752.0 50-51 71673.0 52-53 69229.0 54-55 74150.0 56-57 77359.0 58-59 76528.0 60-61 75347.0 62-63 74087.0 64-65 79735.0 66-67 83930.0 68-69 85503.0 70-71 84389.0 72-73 82353.0 74-75 84463.0 76-77 85348.0 78-79 90034.0 80-81 92484.0 82-83 90828.0 84-85 93358.0 86-87 97975.0 88-89 102962.0 90-91 110341.0 92-93 116363.0 94-95 128603.0 96-97 192187.0 98-99 814909.0 100-101 5978912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.37936281845563 #Duplication Level Percentage of deduplicated Percentage of total 1 89.95293912741336 76.8012462634588 2 6.970969043750746 11.903537903652357 3 1.7289929723573576 4.428609548923767 4 0.6334353606826164 2.1632922992704167 5 0.2662804653012323 1.136742822921055 6 0.1546022633245684 0.7919905639765644 7 0.09081236878310954 0.5427451527915554 8 0.053456841312950115 0.36512888396695814 9 0.03423632586314687 0.26307681186964443 >10 0.1123109444854249 1.4291015968140899 >50 0.0015070891317129095 0.08547133362162024 >100 4.1894030692065336E-4 0.06524492239886112 >500 3.8257286851997054E-5 0.023811896334372893 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0381081185453033E-5 2 0.0 2.0762162370906067E-5 0.0 0.0 2.0762162370906067E-5 3 0.0 2.0762162370906067E-5 0.0 1.0381081185453033E-5 2.0762162370906067E-5 4 0.0 4.152432474181213E-5 0.0 1.0381081185453033E-5 2.0762162370906067E-5 5 0.0 4.152432474181213E-5 0.0 1.0381081185453033E-5 2.0762162370906067E-5 6 4.152432474181213E-5 5.190540592726516E-5 0.0 1.0381081185453033E-5 2.0762162370906067E-5 7 4.152432474181213E-5 5.190540592726516E-5 0.0 2.0762162370906067E-5 3.11432435563591E-5 8 4.152432474181213E-5 7.266756829817123E-5 0.0 2.0762162370906067E-5 3.11432435563591E-5 9 4.152432474181213E-5 9.342973066907729E-5 0.0 3.11432435563591E-5 4.152432474181213E-5 10-11 4.152432474181213E-5 9.342973066907729E-5 0.0 6.747702770544471E-5 4.152432474181213E-5 12-13 4.152432474181213E-5 9.342973066907729E-5 0.0 7.266756829817123E-5 4.152432474181213E-5 14-15 4.152432474181213E-5 1.3495405541088943E-4 0.0 8.823919007635078E-5 4.152432474181213E-5 16-17 8.304864948362427E-5 1.4533513659634246E-4 0.0 9.342973066907729E-5 4.152432474181213E-5 18-19 8.304864948362427E-5 1.7128783955997504E-4 0.0 1.1419189303998336E-4 6.22864871127182E-5 20-21 8.304864948362427E-5 2.1800270489451368E-4 0.0 1.8166892074542804E-4 7.785810889089775E-5 22-23 9.86202712618038E-5 2.543364890435993E-4 0.0 2.3357432667269325E-4 1.1938243363270988E-4 24-25 1.0381081185453032E-4 2.9586081378541146E-4 0.0 3.11432435563591E-4 1.557162177817955E-4 26-27 1.0381081185453032E-4 3.0105135437813793E-4 0.0 4.3600540978902736E-4 1.557162177817955E-4 28-29 1.0381081185453032E-4 3.0624189497086446E-4 0.0 0.001084822983879842 1.557162177817955E-4 30-31 1.0900135244725684E-4 3.529567603054031E-4 0.0 0.0026367946211050706 1.557162177817955E-4 32-33 1.1938243363270988E-4 3.6333784149085614E-4 0.0 0.005792643301482792 1.557162177817955E-4 34-35 1.245729742254364E-4 3.789094632690357E-4 0.0 0.009872408207365834 1.7647838015270154E-4 36-37 1.245729742254364E-4 3.944810850472152E-4 0.0 0.01692635287288117 1.7647838015270154E-4 38-39 1.3495405541088943E-4 3.944810850472152E-4 0.0 0.025599746203327178 1.7647838015270154E-4 40-41 1.557162177817955E-4 4.4638649097448037E-4 0.0 0.03605349495707838 1.7647838015270154E-4 42-43 1.557162177817955E-4 4.6714865334538644E-4 0.0 0.04326834638096824 1.8166892074542804E-4 44-45 1.557162177817955E-4 5.294351404581046E-4 0.0 0.051209873487839806 1.8685946133815458E-4 46-47 1.557162177817955E-4 5.813405463853699E-4 0.0 0.060231033037998497 2.1800270489451368E-4 48-49 1.557162177817955E-4 5.813405463853699E-4 0.0 0.06836980068739368 2.2838378607996672E-4 50-51 1.557162177817955E-4 6.695797364617206E-4 0.0 0.07598432373692347 2.3357432667269325E-4 52-53 1.557162177817955E-4 6.747702770544471E-4 0.0 0.08333412921622421 2.647175702290523E-4 54-55 1.557162177817955E-4 6.799608176471736E-4 0.0 0.09085003199449221 2.906702731926849E-4 56-57 1.557162177817955E-4 6.851513582399001E-4 0.0 0.09762887800859305 2.9586081378541146E-4 58-59 1.557162177817955E-4 6.955324394253531E-4 0.0 0.10347861725659582 3.0105135437813793E-4 60-61 1.557162177817955E-4 7.059135206108062E-4 0.0 0.10982664840150036 3.11432435563591E-4 62-63 1.557162177817955E-4 7.162946017962592E-4 0.0 0.11581653224550675 3.11432435563591E-4 64-65 1.6090675837452203E-4 8.408675760216956E-4 0.0 0.12167665257469498 3.11432435563591E-4 66-67 1.6609729896724853E-4 8.616297383926017E-4 0.0 0.12759386885040322 3.1662297615631747E-4 68-69 1.7647838015270154E-4 8.720108195780547E-4 0.0 0.1336771824250787 3.21813516749044E-4 70-71 1.7647838015270154E-4 8.823919007635078E-4 0.0 0.13969820951264145 3.3219459793449707E-4 72-73 1.920500019308811E-4 8.823919007635078E-4 0.0 0.1442295514500917 3.425756791199501E-4 74-75 2.0243108311633411E-4 8.875824413562343E-4 0.0 0.14784216770262937 3.425756791199501E-4 76-77 2.0762162370906065E-4 9.187256849125933E-4 0.0 0.15112777989782525 3.529567603054031E-4 78-79 2.0762162370906065E-4 9.342973066907729E-4 0.0 0.15399295830501028 3.529567603054031E-4 80-81 2.0762162370906065E-4 9.394878472834994E-4 0.0 0.15540997588682462 3.633378414908561E-4 82-83 2.0762162370906065E-4 9.706310908398585E-4 0.0 0.15587712454017 3.841000038617622E-4 84-85 2.0762162370906065E-4 9.965837938034911E-4 0.0 0.15619893805691903 3.892905444544887E-4 86-87 2.0762162370906065E-4 0.0010484891997307563 0.0 0.15635465427470085 3.944810850472152E-4 88-89 2.0762162370906065E-4 0.0010744419026943888 0.0 0.15640136914003538 4.0486216623266823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12400 0.0 49.436966 1 GTATCAA 23675 0.0 37.543144 1 TCAACGC 29070 0.0 30.136646 4 ATCAACG 29100 0.0 29.973572 3 CAACGCA 29790 0.0 29.365335 5 TATCAAC 30425 0.0 28.836073 2 AACGCAG 30670 0.0 28.577925 6 ACGCAGA 35355 0.0 24.815092 7 CGCAGAG 35560 0.0 24.611454 8 GCAGAGT 41165 0.0 21.19791 9 GTACATG 28160 0.0 20.997057 1 TACATGG 28355 0.0 20.52222 2 ACATGGG 28540 0.0 19.717228 3 GAGTACT 25390 0.0 19.232698 12-13 AGAGTAC 38105 0.0 19.0518 10-11 TGGTATC 6480 0.0 18.881142 2 GTGGTAT 6890 0.0 18.747267 1 GGACCGA 1615 0.0 18.21369 6 CAGAGTA 40810 0.0 18.091919 10-11 ACCGAAC 1405 0.0 17.90135 8 >>END_MODULE