FastQCFastQC Report
Fri 27 May 2016
SRR2139003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9569832
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132400.051.756061
GTATCAA246650.037.6600571
ATCAACG311700.029.2532163
TCAACGC313300.029.171464
CAACGCA319700.028.6140275
TATCAAC324500.028.2439542
AACGCAG327650.027.9323276
ACGCAGA375850.024.3387367
CGCAGAG381250.023.9935028
GTACATG308650.020.9988331
TACATGG306050.020.9263842
GCAGAGT439000.020.7398859
ACATGGG309650.020.1870333
GAGTACT266050.019.6208212-13
GGACCGA17400.019.3717066
AGAGTAC403350.019.34675410-11
AGGACCG21700.019.2691765
ACCGAAC15150.019.1502728
GTGGTAT64200.019.0743831
CCGAACC15400.018.5622449
Produced by FastQC (version 0.11.5)