##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9569832
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50931604650949	34.0	31.0	34.0	31.0	34.0
2	32.642558092973836	34.0	31.0	34.0	31.0	34.0
3	32.635528920465894	34.0	31.0	34.0	31.0	34.0
4	36.19510750031976	37.0	35.0	37.0	35.0	37.0
5	36.1355057225665	37.0	35.0	37.0	35.0	37.0
6	36.163328990519375	37.0	36.0	37.0	35.0	37.0
7	36.11245808703852	37.0	36.0	37.0	35.0	37.0
8	36.10076132997946	37.0	36.0	37.0	35.0	37.0
9	37.805979352615594	39.0	38.0	39.0	35.0	39.0
10-11	37.751099078855304	39.0	38.0	39.0	35.0	39.0
12-13	37.71999576377098	39.0	38.0	39.0	35.0	39.0
14-15	39.05631692384986	40.0	38.0	41.0	36.0	41.0
16-17	39.03438843022532	40.0	38.0	41.0	36.0	41.0
18-19	38.96622866524721	40.0	38.5	41.0	35.0	41.0
20-21	39.02646469009308	40.0	39.0	41.0	35.0	41.0
22-23	38.95950593412701	40.0	38.0	41.0	35.0	41.0
24-25	38.88480479603689	40.0	38.0	41.0	35.0	41.0
26-27	38.80396716533612	40.0	38.0	41.0	35.0	41.0
28-29	38.58996496956818	40.0	38.0	41.0	34.5	41.0
30-31	38.5175569449021	40.0	38.0	41.0	34.0	41.0
32-33	38.44726732816697	40.0	38.0	41.0	34.0	41.0
34-35	38.26143820269397	40.0	38.0	41.0	34.0	41.0
36-37	38.3802911543862	40.0	38.0	41.0	34.0	41.0
38-39	38.502277174292	40.0	38.0	41.0	34.0	41.0
40-41	38.498572534245426	40.0	38.0	41.0	34.0	41.0
42-43	38.373604322063684	40.0	38.0	41.0	34.0	41.0
44-45	38.31582142860983	40.0	38.0	41.0	34.0	41.0
46-47	38.32986580586932	40.0	38.0	41.0	34.0	41.0
48-49	38.208621784040204	40.0	38.0	41.0	33.0	41.0
50-51	38.156551667861194	40.0	37.5	41.0	33.0	41.0
52-53	38.088344009847845	40.0	37.0	41.0	33.0	41.0
54-55	37.98542641353811	40.0	37.0	41.0	33.0	41.0
56-57	37.8424005973806	40.0	37.0	41.0	33.0	41.0
58-59	37.694340975485936	39.5	36.0	41.0	33.0	41.0
60-61	37.523139158136814	39.0	36.0	41.0	33.0	41.0
62-63	37.31400207610277	39.0	35.5	41.0	32.0	41.0
64-65	37.080021110421086	39.0	35.0	40.0	32.0	41.0
66-67	36.810455893810726	38.0	35.0	40.0	32.0	41.0
68-69	36.501438387429474	37.5	35.0	40.0	31.0	41.0
70-71	36.21047042935517	37.0	35.0	39.5	31.0	41.0
72-73	35.888084977969285	36.5	35.0	39.0	31.0	41.0
74-75	35.56333943645011	36.0	34.0	39.0	31.0	40.0
76-77	34.542803241966524	35.0	33.5	37.0	29.5	39.0
78-79	34.851896267726865	35.0	34.0	37.0	30.0	39.0
80-81	34.693509024088364	35.0	34.0	37.0	30.0	39.0
82-83	34.427022778269546	35.0	34.0	36.5	30.0	39.0
84-85	34.15383209304065	35.0	34.0	36.0	30.0	37.0
86-87	33.90371037808954	35.0	34.0	36.0	30.0	37.0
88-89	33.65661136262019	35.0	33.5	35.0	30.0	37.0
90-91	33.52974071754029	35.0	33.0	35.0	30.0	36.0
92-93	33.45101790531953	35.0	33.0	35.0	30.0	36.0
94-95	33.36141311623575	35.0	33.0	35.0	30.0	36.0
96-97	33.34886442641056	35.0	33.0	35.0	30.0	35.5
98-99	33.38042939082864	34.5	33.0	35.0	30.0	35.0
100-101	32.16103557666392	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	1.0
19	20.0
20	62.0
21	314.0
22	1462.0
23	4741.0
24	11663.0
25	24240.0
26	42023.0
27	65238.0
28	93157.0
29	123675.0
30	158914.0
31	199843.0
32	251438.0
33	326258.0
34	451240.0
35	641179.0
36	1052611.0
37	2038879.0
38	2746818.0
39	1281022.0
40	55030.0
41	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.88320261521914	21.726657811754002	15.434748102604551	23.955391470422303
2	13.660651616658981	22.206248408409387	41.56827671633422	22.56482325859741
3	16.737056673316424	27.439155583249224	31.620433788582535	24.20335395485182
4	11.812891125924367	19.155546276372526	39.72687853298624	29.304684064716866
5	12.469842579519035	38.638458297491354	36.08026053670595	12.81143858628366
6	28.58651920825046	39.35569563984345	19.147256461319856	12.910528690586235
7	25.29620729886886	33.54503574613893	24.056934667354263	17.101822287637944
8	23.918255088680294	37.1823356256211	21.558125221400307	17.341284064298307
9	25.330276351965548	16.82180244517408	21.858203979209993	35.98971722365039
10-11	23.506944382763272	27.319669824545308	29.590078017133237	19.583307775558183
12-13	24.472885098272386	25.115895475677206	29.639857761318645	20.771361664731767
14-15	21.659326314216546	26.357811943644695	27.51615169254795	24.46671004959081
16-17	20.91533810862727	29.45670168241204	28.11335310223864	21.514607106722046
18-19	20.988091845737262	28.578311064910356	30.030317156137272	20.403279933215106
20-21	21.867389714490056	27.635538419152155	29.966372821377185	20.5306990449806
22-23	21.51962829702063	27.554769518495398	29.83260772381568	21.092994460668287
24-25	21.286001798949723	27.762121491469106	29.864180707097958	21.087696002483213
26-27	21.196192591946975	27.91504057807865	29.795835451911156	21.092931378063223
28-29	21.166422669011606	27.90561743529208	29.76094947020838	21.167010425487934
30-31	21.47342024102903	27.817633010394527	29.699917678831905	21.009029069744543
32-33	21.271030670005057	27.965986393483682	29.61266024241703	21.150322694094232
34-35	21.417970227543805	28.00657392702519	29.613701392412572	20.96175445301843
36-37	21.40808819000415	27.982574454753816	29.54159366325737	21.06774369198466
38-39	21.243515879861906	28.10840166770815	29.544922511614914	21.10315994081503
40-41	21.437048155442152	28.02960804894081	29.39314421953209	21.140199576084953
42-43	21.427603819334866	28.15710483683273	29.085007083784504	21.3302842600479
44-45	21.510730858941404	28.230077917646685	28.888148161429328	21.37104306198259
46-47	21.520276418084876	28.260134916364894	28.673437289748822	21.546151375801408
48-49	21.539634929273692	28.418880642360122	28.550921630249665	21.49056279811652
50-51	21.57716162671673	28.4930785959063	28.391779125246185	21.537980652130777
52-53	21.657052333780594	28.409552616897386	28.28698036101432	21.646414688307697
54-55	21.664540197753947	28.3314614996376	28.360979131811582	21.643019170796872
56-57	21.64691751693502	28.418142871181463	28.283265975966778	21.651673635916747
58-59	21.597220887020704	28.50777936038853	28.31987536810423	21.575124384486536
60-61	21.695384363202148	28.46552557028266	28.31548238000258	21.5236076865126
62-63	21.673503833284656	28.507468237713766	28.229098931770935	21.589928997230643
64-65	21.630623698274935	28.430392673009035	28.236626869893342	21.702356758822685
66-67	21.760327282957885	28.47322952591837	28.04800298470107	21.718440206422674
68-69	21.660719399738255	28.401082883618063	28.19642951175259	21.74176820489109
70-71	21.63527549504541	28.282597451846986	28.235939976177686	21.84618707692992
72-73	21.740060739880096	28.304668098442075	28.183735144054634	21.771536017623195
74-75	21.56802944593939	28.392256099059036	28.158496405371036	21.881218049630537
76-77	21.67481866760949	28.31507247308453	28.133528956146836	21.876579903159143
78-79	21.681491806234032	28.30365087342755	28.123840018617692	21.891017301720726
80-81	21.696939999226295	28.32857934983043	28.09617145288785	21.878309198055423
82-83	21.70253381919969	28.296777747594028	28.191594289280154	21.80909414392613
84-85	21.852183842748822	28.266944338540252	28.07472520476529	21.806146613945643
86-87	21.66437473831217	28.300075847667916	28.167576526551365	21.867972887468547
88-89	21.723639891301804	28.1963821276414	28.149822367192318	21.93015561386448
90-91	21.777885161832288	28.22528202585567	28.184985494555498	21.81184731775655
92-93	21.81517634837667	28.141966584622935	28.192061982469824	21.85079508453057
94-95	21.663013458590513	28.13323139832327	28.289558907012065	21.914196236074147
96-97	21.625018369346915	28.213592244266085	28.29316376240166	21.86822562398534
98-99	21.81395085099802	28.799171283757648	28.344389267406818	21.042488597837515
100-101	22.279202371115264	31.542935627949326	25.584609450858203	20.593252550077214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2428.0
1	2227.0
2	3161.5
3	4605.0
4	6016.0
5	8678.0
6	11045.5
7	13744.0
8	16367.5
9	17286.0
10	16236.0
11	14556.5
12	15286.0
13	19805.0
14	31549.5
15	47763.5
16	60522.5
17	66982.0
18	67491.0
19	64081.0
20	58709.0
21	53399.5
22	50962.5
23	54362.5
24	63773.5
25	79079.5
26	98653.0
27	120044.5
28	143160.0
29	167651.5
30	190995.5
31	213438.5
32	236797.0
33	261206.0
34	285073.5
35	305146.5
36	326245.5
37	345060.5
38	356476.5
39	364346.5
40	372720.5
41	380480.0
42	383550.0
43	386133.0
44	388097.0
45	386710.0
46	382440.5
47	377493.0
48	371554.5
49	362125.5
50	350211.5
51	334893.5
52	316322.0
53	296986.5
54	277846.5
55	256284.5
56	233760.5
57	210614.5
58	186033.5
59	160376.0
60	134132.5
61	109722.5
62	85803.5
63	64417.0
64	48135.0
65	37040.5
66	28776.5
67	21853.5
68	16729.5
69	11732.0
70	7585.0
71	5311.0
72	3773.0
73	2674.5
74	1828.0
75	1152.5
76	698.0
77	451.0
78	305.0
79	211.5
80	149.5
81	105.0
82	73.0
83	50.5
84	36.5
85	28.0
86	21.5
87	16.5
88	12.5
89	8.0
90	4.5
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.1348512701163404E-5
2	3.1348512701163404E-5
3	5.747227328546624E-4
4	2.5078810160930723E-4
5	5.015762032186145E-4
6	3.9708116088140317E-4
7	0.0027900176304035433
8	6.478692624907104E-4
9	4.493286820500088E-4
10-11	1.1494454657093249E-4
12-13	2.089900846744227E-5
14-15	1.5151781138895645E-4
16-17	9.352306289180416E-4
18-19	3.1348512701163404E-5
20-21	1.044987495418383E-5
22-23	0.002894864327793953
24-25	0.0073334729289263635
26-27	0.036450922702549855
28-29	0.2809015076463999
30-31	0.24615651231107366
32-33	0.25307413809631757
34-35	0.13365615363208108
36-37	0.024356880308609285
38-39	0.015322256063226123
40-41	0.008776636369354232
42-43	0.005768388070003994
44-45	0.006437937871454754
46-47	0.001393880731881773
48-49	0.0019502148920736118
50-51	0.002788186136847975
52-53	0.0017738480067917542
54-55	2.1566383456540758E-4
56-57	0.0018085516448805828
58-59	0.0031928340102757293
60-61	0.012603949823932023
62-63	0.0078962113519671
64-65	0.0021930796837128625
66-67	0.0017415240741465545
68-69	0.002551225062606277
70-71	0.004324700729793248
72-73	0.003894142518457372
74-75	0.004034262171796093
76-77	0.0016448683793338736
78-79	9.558635730333299E-5
80-81	4.5132551078764686E-4
82-83	3.915258408033745E-5
84-85	0.0
86-87	1.3377011952761491E-5
88-89	0.0
90-91	5.777247096365926E-4
92-93	0.008963268414259491
94-95	0.0012861325071767615
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	209.0
20-21	667.0
22-23	1482.0
24-25	5327.0
26-27	5750.0
28-29	8235.0
30-31	18876.0
32-33	24301.0
34-35	34509.0
36-37	47860.0
38-39	58818.0
40-41	63178.0
42-43	75446.0
44-45	90704.0
46-47	89848.0
48-49	78090.0
50-51	70949.0
52-53	67905.0
54-55	72295.0
56-57	76284.0
58-59	75527.0
60-61	74960.0
62-63	73732.0
64-65	79339.0
66-67	83818.0
68-69	84861.0
70-71	84369.0
72-73	82710.0
74-75	84902.0
76-77	85991.0
78-79	90554.0
80-81	93158.0
82-83	91014.0
84-85	94569.0
86-87	98743.0
88-89	103525.0
90-91	111877.0
92-93	117164.0
94-95	129323.0
96-97	191720.0
98-99	809277.0
100-101	5937966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.20912283921786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40744837141678	75.28922802649663
2	7.222374669279681	12.163796714324562
3	1.8321757980925195	4.62857750533845
4	0.6901458524882611	2.3246630747664283
5	0.32414788123682514	1.3648104374572003
6	0.1863236727690746	0.9414091828839141
7	0.10291298003680988	0.6066348244368789
8	0.06155140484177947	0.41465518489982683
9	0.036513200035655995	0.27672700923499405
>10	0.1337682352848216	1.7461961798071322
>50	0.0019144545829717424	0.10686947376993611
>100	6.608649833636239E-4	0.09512952693970048
>500	4.174385333380317E-5	0.022637818150290402
>1k	2.0871098101882372E-5	0.01866504149409528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.089900846744227E-5	0.0	2.089900846744227E-5	0.0
3	0.0	2.089900846744227E-5	0.0	2.089900846744227E-5	0.0
4	0.0	3.1348512701163404E-5	0.0	2.089900846744227E-5	0.0
5	2.089900846744227E-5	3.1348512701163404E-5	0.0	2.089900846744227E-5	0.0
6	2.089900846744227E-5	3.1348512701163404E-5	0.0	2.089900846744227E-5	1.0449504233721135E-5
7	2.089900846744227E-5	3.1348512701163404E-5	0.0	2.089900846744227E-5	1.0449504233721135E-5
8	2.089900846744227E-5	3.1348512701163404E-5	0.0	2.089900846744227E-5	2.089900846744227E-5
9	2.089900846744227E-5	3.1348512701163404E-5	0.0	2.089900846744227E-5	2.089900846744227E-5
10-11	1.0449504233721135E-4	3.1348512701163404E-5	0.0	3.1348512701163404E-5	3.1348512701163404E-5
12-13	1.5674256350581704E-4	3.1348512701163404E-5	0.0	4.179801693488454E-5	3.1348512701163404E-5
14-15	1.6719206773953816E-4	6.269702540232681E-5	0.0	5.224752116860567E-5	4.702276905174511E-5
16-17	1.776415719732593E-4	6.269702540232681E-5	0.0	7.314652963604794E-5	6.269702540232681E-5
18-19	1.9854058044070156E-4	6.269702540232681E-5	0.0	8.359603386976908E-5	7.83712817529085E-5
20-21	2.089900846744227E-4	6.269702540232681E-5	0.0	9.404553810349022E-5	8.359603386976908E-5
22-23	2.1943958890814386E-4	8.882078598662966E-5	0.0	1.462930592720959E-4	9.927029022035078E-5
24-25	2.8736136642733127E-4	1.1494454657093249E-4	0.0	1.8286632409011987E-4	1.2016929868779305E-4
26-27	3.50058391829658E-4	1.2539405080465361E-4	0.0	2.612376058430284E-4	1.2539405080465361E-4
28-29	3.552831439465186E-4	1.2539405080465361E-4	0.0	7.00116783659316E-4	1.306188029215142E-4
30-31	3.552831439465186E-4	1.462930592720959E-4	0.0	0.001812988984550617	1.3584355503837477E-4
32-33	3.6573264818023975E-4	1.462930592720959E-4	0.0	0.0047179511615250925	1.3584355503837477E-4
34-35	4.0753066511512424E-4	1.619673156226776E-4	0.0	0.008437974668729817	1.462930592720959E-4
36-37	4.3887917781628767E-4	1.8809107620698044E-4	0.0	0.01528239994181716	1.5674256350581704E-4
38-39	4.3887917781628767E-4	1.93315828323841E-4	0.0	0.022858290511264985	1.5674256350581704E-4
40-41	4.3887917781628767E-4	2.1943958890814383E-4	0.0	0.032973410609507044	1.619673156226776E-4
42-43	4.545534341668694E-4	2.403385973755861E-4	0.0	0.040277614068878115	1.776415719732593E-4
44-45	4.702276905174511E-4	2.7168711007674953E-4	0.0	0.047916201663728265	1.9854058044070156E-4
46-47	4.859019468680328E-4	2.8213661431047065E-4	0.0	0.05655271691289879	2.2988909314186498E-4
48-49	4.911266989848933E-4	2.8213661431047065E-4	0.0	0.06460405992498092	2.5078810160930723E-4
50-51	4.911266989848933E-4	2.9781087066105234E-4	0.0	0.07214337722961073	2.5078810160930723E-4
52-53	5.172504595691962E-4	3.030356227779129E-4	0.0	0.07942668168051435	2.7691186219361004E-4
54-55	5.224752116860568E-4	3.1870987912849464E-4	0.0	0.08695032472879356	3.1870987912849464E-4
56-57	5.224752116860568E-4	3.4483363971279745E-4	0.0	0.09346036586640183	3.343841354790763E-4
58-59	5.329247159197779E-4	3.4483363971279745E-4	0.0	0.09951585356984324	3.343841354790763E-4
60-61	5.485989722703597E-4	3.50058391829658E-4	0.0	0.10554521751270032	3.552831439465186E-4
62-63	5.538237243872202E-4	3.7618215241396087E-4	0.0	0.11120362405526033	3.7618215241396087E-4
64-65	5.538237243872202E-4	4.336544256994271E-4	0.0	0.11747855134760986	3.8140690453082143E-4
66-67	5.747227328546624E-4	4.3887917781628767E-4	0.0	0.12339819549601289	3.970811608814031E-4
68-69	5.747227328546624E-4	4.545534341668694E-4	0.0	0.1292812663795979	4.179801693488454E-4
70-71	5.747227328546624E-4	4.6500293840059053E-4	0.0	0.13468888482054858	4.179801693488454E-4
72-73	5.799474849715229E-4	4.702276905174511E-4	0.0	0.1390985756071789	4.806771947511722E-4
74-75	5.851722370883836E-4	4.806771947511722E-4	0.0	0.14273500308051384	4.911266989848933E-4
76-77	5.956217413221047E-4	4.859019468680328E-4	0.0	0.14619378898187554	4.96351451101754E-4
78-79	6.060712455558258E-4	4.96351451101754E-4	0.0	0.1491666729363692	5.015762032186145E-4
80-81	6.060712455558258E-4	5.06800955335475E-4	0.0	0.15038926493171456	5.120257074523356E-4
82-83	6.16520749789547E-4	5.120257074523356E-4	0.0	0.15071842431507682	5.538237243872202E-4
84-85	6.269702540232682E-4	5.224752116860568E-4	0.0	0.15109983121960763	5.538237243872202E-4
86-87	6.269702540232682E-4	5.224752116860568E-4	0.0	0.15121477576617856	5.538237243872202E-4
88-89	6.269702540232682E-4	5.329247159197779E-4	0.0	0.1512461242788797	5.538237243872202E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13240	0.0	51.75606	1
GTATCAA	24665	0.0	37.660057	1
ATCAACG	31170	0.0	29.253216	3
TCAACGC	31330	0.0	29.17146	4
CAACGCA	31970	0.0	28.614027	5
TATCAAC	32450	0.0	28.243954	2
AACGCAG	32765	0.0	27.932327	6
ACGCAGA	37585	0.0	24.338736	7
CGCAGAG	38125	0.0	23.993502	8
GTACATG	30865	0.0	20.998833	1
TACATGG	30605	0.0	20.926384	2
GCAGAGT	43900	0.0	20.739885	9
ACATGGG	30965	0.0	20.187033	3
GAGTACT	26605	0.0	19.62082	12-13
GGACCGA	1740	0.0	19.371706	6
AGAGTAC	40335	0.0	19.346754	10-11
AGGACCG	2170	0.0	19.269176	5
ACCGAAC	1515	0.0	19.150272	8
GTGGTAT	6420	0.0	19.074383	1
CCGAACC	1540	0.0	18.562244	9
>>END_MODULE