FastQCFastQC Report
Fri 27 May 2016
SRR2139002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16584694
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277800.055.672261
GTATCAA526350.038.4331861
ATCAACG684550.029.2253323
TATCAAC722650.027.8243772
TCAACGC720750.027.7808514
CAACGCA737000.027.1397425
AACGCAG743800.026.9255986
ACGCAGA749200.026.7202057
GTGGTAT110850.024.583121
TGGTATC111850.023.0455172
CGCAGAG895350.022.3626358
GTACATG628900.020.7944971
TACATGG632400.020.2666112
GCAGAGT990000.020.1096469
ACATGGG630500.019.8934753
AGAGTAC916750.019.55973610-11
GAGTACT611900.019.30003712-13
AGTACTT657100.018.4916212-13
CAGAGTA965800.018.1760610-11
GTACTTT677700.017.42705514-15
Produced by FastQC (version 0.11.5)