##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16584694
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43576257722934	34.0	31.0	34.0	31.0	34.0
2	32.58123918355081	34.0	31.0	34.0	31.0	34.0
3	33.249899817265245	34.0	31.0	34.0	31.0	37.0
4	36.07403157393196	37.0	35.0	37.0	35.0	37.0
5	36.02083758675318	37.0	35.0	37.0	35.0	37.0
6	36.0508807096471	37.0	35.0	37.0	35.0	37.0
7	36.057741674341415	37.0	35.0	37.0	35.0	37.0
8	36.32090836285553	37.0	36.0	37.0	35.0	39.0
9	37.637676763888436	39.0	37.0	39.0	35.0	39.0
10-11	37.586481004714344	39.0	37.0	39.0	35.0	39.0
12-13	37.70929674674733	39.0	37.0	39.0	35.0	39.5
14-15	38.79092170769023	40.0	38.0	41.0	35.0	41.0
16-17	38.74581128840846	40.0	38.0	41.0	34.5	41.0
18-19	38.7189152540288	40.0	38.0	41.0	34.0	41.0
20-21	38.76109195443731	40.0	38.0	41.0	34.0	41.0
22-23	38.680067992983844	40.0	38.0	41.0	34.0	41.0
24-25	38.576202051590855	40.0	38.0	41.0	34.0	41.0
26-27	38.4908410134452	40.0	38.0	41.0	34.0	41.0
28-29	38.26865079586971	40.0	38.0	41.0	34.0	41.0
30-31	38.1864204468511	40.0	38.0	41.0	34.0	41.0
32-33	38.07125739868812	40.0	37.0	41.0	33.5	41.0
34-35	37.902076180150516	40.0	37.0	41.0	33.0	41.0
36-37	38.039058701140846	40.0	37.0	41.0	33.0	41.0
38-39	38.13330864682129	40.0	38.0	41.0	33.0	41.0
40-41	38.10659371749297	40.0	37.0	41.0	33.0	41.0
42-43	37.999062347215926	40.0	37.0	41.0	33.0	41.0
44-45	37.94089609550424	40.0	37.0	41.0	33.0	41.0
46-47	37.92934070131281	40.0	37.0	41.0	33.0	41.0
48-49	37.8248851570195	40.0	37.0	41.0	33.0	41.0
50-51	37.74172307606459	40.0	37.0	41.0	33.0	41.0
52-53	37.65563671917366	40.0	36.5	41.0	33.0	41.0
54-55	37.535505096980124	39.5	36.0	41.0	32.0	41.0
56-57	37.36814758645461	39.0	36.0	41.0	32.0	41.0
58-59	37.200550643722934	39.0	35.5	40.5	32.0	41.0
60-61	37.00466890671139	39.0	35.0	40.0	31.0	41.0
62-63	36.777338222170954	38.0	35.0	40.0	31.0	41.0
64-65	36.51850964909281	38.0	35.0	40.0	31.0	41.0
66-67	36.22847378783989	37.0	35.0	40.0	31.0	41.0
68-69	35.92406160216627	37.0	34.0	39.5	31.0	41.0
70-71	35.6220274885097	36.0	34.0	39.0	30.0	40.5
72-73	35.2968553512178	36.0	34.0	39.0	30.0	40.0
74-75	34.878374343192874	35.0	34.0	38.0	29.5	40.0
76-77	34.10625745366223	35.0	32.5	37.0	29.0	39.0
78-79	34.383015242709455	35.0	34.0	37.0	29.5	39.0
80-81	34.23692048079231	35.0	34.0	36.5	30.0	39.0
82-83	33.98870096926247	35.0	33.5	36.0	29.0	37.5
84-85	33.731624371420025	35.0	33.0	36.0	29.0	37.0
86-87	33.49371267362518	35.0	33.0	35.0	29.0	37.0
88-89	33.274467190750514	35.0	33.0	35.0	29.0	36.0
90-91	33.16539575993086	35.0	33.0	35.0	29.0	36.0
92-93	33.096873851598005	34.0	33.0	35.0	29.0	36.0
94-95	33.027729660243196	34.0	33.0	35.0	29.0	35.0
96-97	33.03697075989577	34.0	33.0	35.0	29.0	35.0
98-99	33.053527359505495	34.0	32.5	35.0	29.0	35.0
100-101	31.57288054682661	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	10.0
19	29.0
20	132.0
21	771.0
22	3335.0
23	10563.0
24	25893.0
25	51740.0
26	89020.0
27	136114.0
28	190821.0
29	252035.0
30	319791.0
31	400096.0
32	499218.0
33	647866.0
34	893799.0
35	1295429.0
36	2163075.0
37	3857590.0
38	4074488.0
39	1603543.0
40	69333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89031823267835	0.01915622294756326	27.529427895814024	30.561097648560064
2	14.421486830910716	25.766554293814707	37.16586462076122	22.646094254513354
3	15.18755054003941	25.90023903467688	34.38381445270116	24.528395972582555
4	12.807711071541778	24.772710321150136	36.85412428692444	25.565454320383648
5	17.193993960676867	36.90707787446698	31.98523453026415	13.91369363459201
6	26.9580936531481	38.37198967717039	20.320164489948507	14.349752179733006
7	25.365422162299705	33.454581962063095	22.767592155992567	18.41240371964463
8	24.043833409407878	32.09310318421718	22.386586118093824	21.476477288281124
9	23.696888073462645	20.579836313660238	23.880358977815987	31.84291663506113
10-11	24.528595007994728	26.374310673984745	28.61079666424636	20.486297653774162
12-13	24.876476407210845	24.801440316687334	28.680883677870057	21.64119959823177
14-15	22.259886535540108	26.993699510773943	27.11491088649999	23.631503067185953
16-17	20.94584733168029	29.217924094703218	28.02148807496858	21.814740498647915
18-19	21.130533346562405	28.000868632691283	30.247965811591666	20.620632209154643
20-21	22.04257130966019	27.121775723510382	30.188950082484123	20.64670288434531
22-23	21.889579736897716	26.987840831127624	29.961383448467522	21.161195983507135
24-25	21.37354840219871	27.346073078335724	30.19268526468659	21.087693254778973
26-27	21.412746696125236	27.485831865644133	30.01730546063125	21.08411597759938
28-29	21.40220635746334	27.350605788463646	29.99515841578762	21.252029438285394
30-31	21.499183219047058	27.362604541110496	30.01546918005524	21.1227430597872
32-33	21.397064883055016	27.623291612385355	29.84077089635091	21.138872608208718
34-35	21.648311614978617	27.430920236491684	29.747147536175504	21.173620612354192
36-37	21.53121240986699	27.593304545554876	29.693436023317112	21.182047021261024
38-39	21.417430305499824	27.825331426881366	29.608379505985837	21.148858761632976
40-41	21.68784645950247	27.521806435459467	29.369538828505203	21.42080827653286
42-43	21.569544296433758	27.752543739121784	29.16016401698331	21.517747947461153
44-45	21.742414465567744	28.022012930257826	28.66642698950429	21.569145614670134
46-47	21.930230004242574	27.913479598751263	28.31807013924855	21.838220257757616
48-49	21.858249488628122	28.216023092082203	28.125800469416017	21.799926949873655
50-51	21.96754689977588	28.395965620070086	27.810455683128986	21.826031797025045
52-53	22.108409852680985	28.12562114038623	27.73239584023033	22.033573166702457
54-55	21.994400264335347	28.16442453648151	27.866659710473296	21.97451548870985
56-57	21.962607423385712	28.430049045305513	27.687476590798298	21.919866940510474
58-59	22.082123659452883	28.174723268610663	27.735914935870948	22.00723813606551
60-61	22.016094912226936	28.265716243528953	27.836354841647342	21.88183400259677
62-63	22.024882556129423	28.447400148126878	27.722643110976097	21.805074184767605
64-65	22.18705317485138	28.167185334290163	27.576681586761275	22.06907990409718
66-67	21.985315249471096	28.288887305483158	27.66874948292393	22.05704796212182
68-69	22.068333994552162	28.350943354581005	27.59348936211154	21.987233288755302
70-71	22.072429989431395	28.03030920750342	27.649003306765913	22.248257496299274
72-73	22.037403452668684	28.13663740961469	27.663351712831417	22.162607424885213
74-75	21.981893010856645	28.36631213069983	27.594988706899805	22.05680615154372
76-77	22.19895783690778	27.983859916592184	27.517026182789646	22.30015606371039
78-79	21.927646691441975	28.15470059323885	27.675148758921083	22.242503956398092
80-81	22.069035089002924	28.193239078960968	27.596672727497428	22.141053104538678
82-83	22.191407846648474	27.898235835077127	27.68221007618945	22.228146242084943
84-85	22.110287273345833	27.981254483126015	27.77440232897667	22.13405591455148
86-87	22.101550812172015	28.036924985983973	27.693617197871234	22.167907003972772
88-89	22.172920303240897	27.794469381874894	27.69974313296749	22.332867181916715
90-91	22.14259143291286	27.852342209188315	27.794763488949098	22.210302868949736
92-93	22.163901072659232	27.8650323353908	27.811995341518507	22.159071250431456
94-95	22.092507852390707	27.690482578833926	27.88953231308196	22.327477255693402
96-97	21.967053771426983	27.877750735636326	27.981227375976204	22.173968116960488
98-99	22.4282661838734	28.830749451310062	27.858115247298066	20.882869117518474
100-101	22.72223689936838	30.87634783200461	24.749760189227228	21.65165507939978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6285.0
1	5314.0
2	7165.5
3	10273.5
4	13084.0
5	19287.5
6	24073.0
7	29164.5
8	33853.0
9	34017.0
10	30277.5
11	26019.5
12	28245.5
13	39154.0
14	69929.5
15	119081.5
16	161721.5
17	182345.0
18	181248.5
19	166612.0
20	145505.0
21	123065.0
22	106555.0
23	103089.0
24	112587.0
25	133624.0
26	161587.0
27	193314.0
28	231461.5
29	271225.5
30	309598.5
31	346850.5
32	383490.5
33	420309.0
34	453916.5
35	482364.5
36	510739.5
37	537483.0
38	560803.5
39	579563.0
40	591499.0
41	603421.0
42	612646.5
43	621015.0
44	631494.5
45	640844.5
46	650557.5
47	661138.0
48	666638.5
49	659745.5
50	645673.5
51	628673.0
52	601892.0
53	564204.0
54	528017.5
55	491978.0
56	450757.5
57	407716.0
58	362943.5
59	312615.0
60	258744.5
61	212021.5
62	170307.0
63	132592.0
64	99947.5
65	73455.0
66	55181.5
67	41274.5
68	30016.5
69	21186.0
70	14867.0
71	10447.0
72	7153.5
73	4773.0
74	3238.0
75	2383.5
76	1874.5
77	1208.5
78	731.0
79	537.0
80	346.0
81	221.5
82	162.5
83	110.5
84	73.0
85	54.5
86	42.0
87	31.5
88	18.0
89	8.0
90	5.5
91	4.5
92	3.5
93	3.0
94	2.5
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.014828009488749E-5
2	1.4471174445545996E-4
3	4.2810557734740233E-4
4	1.2662277639852745E-4
5	3.256014250247849E-4
6	5.667876657838847E-4
7	0.0018933119899589344
8	5.064911055941098E-4
9	2.894234889109199E-4
10-11	9.647449630363997E-5
12-13	4.823724815181999E-5
14-15	1.7787485255983622E-4
16-17	8.833446067802034E-4
18-19	5.125207616130873E-5
20-21	1.7486916620632862E-4
22-23	0.0031691453695424023
24-25	0.010227888886217888
26-27	0.06828193513545094
28-29	0.25235025392530996
30-31	0.22732097180396013
32-33	0.2257387261612435
34-35	0.09712022678543365
36-37	0.019687521348398063
38-39	0.011870855426143414
40-41	0.0060453846724412685
42-43	0.003647409199652872
44-45	0.004206955710508426
46-47	6.931427388842185E-4
48-49	0.0010787195598824196
50-51	0.0016786220410049228
52-53	6.933229972346599E-4
54-55	1.0268655862097385E-4
56-57	9.789586372601748E-4
58-59	0.0024575791166860585
60-61	0.009622914414431881
62-63	0.005583877249423937
64-65	0.0012782352732314498
66-67	0.0018547900934051294
68-69	0.0024724350900014124
70-71	0.00386937864573607
72-73	0.003540142375920089
74-75	0.003151905692641514
76-77	0.0013166950908835842
78-79	1.0855844455364342E-4
80-81	3.3739968188138306E-4
82-83	3.840116444618973E-6
84-85	3.890757944159297E-6
86-87	0.0
88-89	6.405557718098531E-5
90-91	0.0014196176680815115
92-93	0.006977302578133995
94-95	8.312129667197518E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	511.0
20-21	1723.0
22-23	3393.0
24-25	7666.0
26-27	12100.0
28-29	20900.0
30-31	35717.0
32-33	49776.0
34-35	72721.0
36-37	94559.0
38-39	111128.0
40-41	126196.0
42-43	159320.0
44-45	188873.0
46-47	180213.0
48-49	148034.0
50-51	125126.0
52-53	120820.0
54-55	127862.0
56-57	133408.0
58-59	132555.0
60-61	130821.0
62-63	132327.0
64-65	144183.0
66-67	150775.0
68-69	153457.0
70-71	155487.0
72-73	152243.0
74-75	155013.0
76-77	159491.0
78-79	166886.0
80-81	168798.0
82-83	168737.0
84-85	174227.0
86-87	182637.0
88-89	194161.0
90-91	207000.0
92-93	223385.0
94-95	266824.0
96-97	452848.0
98-99	1672044.0
100-101	9520749.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.79730351528868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.72649145256152	70.8806395622399
2	7.808005004913975	12.617315004618549
3	2.3172496482422513	5.616825694491752
4	0.9141387449586532	2.954397825260375
5	0.45635664312598506	1.8436193102934244
6	0.24754331546907224	1.200049943588129
7	0.15231907877617232	0.8614879587333292
8	0.0972394774225995	0.6285350056785466
9	0.06747360539960744	0.49065168372686124
>10	0.2082175254995725	2.557842761267505
>50	0.003993737144948694	0.21845645890791016
>100	9.418537775911265E-4	0.11519626754377711
>500	2.991270800999166E-5	0.014982523649935934
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2059312037954996E-4	0.0	0.0	0.0
3	0.0	1.2662277639852745E-4	0.0	1.2059312037954997E-5	0.0
4	0.0	1.2662277639852745E-4	0.0	1.2059312037954997E-5	0.0
5	0.0	1.3265243241750496E-4	0.0	1.2059312037954997E-5	0.0
6	6.632621620875248E-5	1.3265243241750496E-4	0.0	1.2059312037954997E-5	0.0
7	6.632621620875248E-5	1.4471174445545996E-4	0.0	1.8088968056932494E-5	0.0
8	7.838552824670747E-5	1.5074140047443747E-4	0.0	1.8088968056932494E-5	0.0
9	8.441518426568497E-5	2.472158967780774E-4	0.0	1.8088968056932494E-5	6.0296560189774984E-6
10-11	8.743001227517372E-5	2.472158967780774E-4	0.0	3.0148280094887493E-5	6.0296560189774984E-6
12-13	9.044484028466248E-5	2.472158967780774E-4	0.0	3.617793611386499E-5	9.044484028466247E-6
14-15	1.0250415232261747E-4	3.376607370627399E-4	0.0	4.522242014233124E-5	2.713345208539874E-5
16-17	1.8993416459779118E-4	3.919276412335374E-4	0.0	5.728173218028623E-5	3.617793611386499E-5
18-19	2.019934766357462E-4	3.919276412335374E-4	0.0	9.345966829415122E-5	3.617793611386499E-5
20-21	2.1103796066421242E-4	3.979572972525149E-4	0.0	1.3868208843648245E-4	3.617793611386499E-5
22-23	2.2912692872114493E-4	5.004614495751323E-4	0.0	1.5978588450290372E-4	3.919276412335374E-5
24-25	2.4118624075909992E-4	5.275949016605311E-4	0.0	2.0500830464523494E-4	4.2207592132842485E-5
26-27	2.4118624075909992E-4	5.547283537459298E-4	0.0	3.3464590905325114E-4	4.2207592132842485E-5
28-29	2.5023072478756617E-4	5.667876657838849E-4	0.0	9.49670822988956E-4	4.2207592132842485E-5
30-31	2.713345208539874E-4	6.60247334078036E-4	0.0	0.0025294406999610605	4.2207592132842485E-5
32-33	2.954531449298974E-4	6.72306646115991E-4	0.0	0.0053633790288804845	4.2207592132842485E-5
34-35	3.075124569678524E-4	7.597366583911647E-4	0.0	0.009632375490316553	5.426690417079748E-5
36-37	3.1655694099631864E-4	8.441518426568497E-4	0.0	0.016397649543609307	5.426690417079748E-5
38-39	3.3464590905325114E-4	8.501814986758273E-4	0.0	0.024073401655767664	5.426690417079748E-5
40-41	3.4670522109120616E-4	8.984187468276473E-4	0.0	0.03302442601593976	5.426690417079748E-5
42-43	3.557497051196724E-4	0.0010069525551692424	0.0	0.03997058974980183	5.426690417079748E-5
44-45	3.7082384516711614E-4	0.0011154863635108373	0.0	0.04790561707077622	5.426690417079748E-5
46-47	3.858979852145599E-4	0.0011154863635108373	0.0	0.055928074404025786	6.331138819926373E-5
48-49	4.0700178128098114E-4	0.0011245308475393034	0.0	0.06355558926803231	6.632621620875248E-5
50-51	4.3413523336637987E-4	0.0012873315600516958	0.0	0.07070374647853014	6.632621620875248E-5
52-53	4.4317971739484614E-4	0.0013024057000991397	0.0	0.07774638470869585	7.838552824670749E-5
54-55	4.461945454043349E-4	0.0013144650121370946	0.0	0.0851869802361141	9.044484028466248E-5
56-57	4.461945454043349E-4	0.0013506429482509595	0.0	0.0920185805056156	9.044484028466248E-5
58-59	4.5222420142331237E-4	0.0013657170882984033	0.0	0.09795477685629894	9.345966829415122E-5
60-61	4.6428351346126733E-4	0.001392850540383802	0.0	0.10361963868612831	9.647449630363997E-5
62-63	5.095059336035987E-4	0.0014109395084407346	0.0	0.10939001949628978	9.647449630363997E-5
64-65	5.245800736510424E-4	0.0016038885010480144	0.0	0.11524180066270742	9.647449630363997E-5
66-67	5.607580097649074E-4	0.001621977469104947	0.0	0.12143124256618784	9.647449630363997E-5
68-69	5.637728377743961E-4	0.0016732295452662558	0.0	0.12737648340089963	9.647449630363997E-5
70-71	5.698024937933736E-4	0.001706392653370632	0.0	0.13252580964110644	1.0853380834159497E-4
72-73	6.331138819926374E-4	0.001718451965408587	0.0	0.13661090159396366	1.3868208843648245E-4
74-75	6.512028500495698E-4	0.0017516150735129633	0.0	0.13993927171643927	1.3868208843648245E-4
76-77	6.60247334078036E-4	0.0018601488818545582	0.0	0.14291490696180464	1.3868208843648245E-4
78-79	6.662769900970135E-4	0.0019114009580158669	0.0	0.14544434766176573	1.3868208843648245E-4
80-81	6.692918181065023E-4	0.0019174306140348444	0.0	0.14653571540120064	1.477265724649487E-4
82-83	6.692918181065023E-4	0.0019264750980633105	0.0	0.14699095443063345	1.5677105649341495E-4
84-85	6.813511301444572E-4	0.0019415492381107545	0.0	0.14739795621191443	1.5677105649341495E-4
86-87	6.813511301444572E-4	0.0019716975182056417	0.0	0.1475215641603035	1.5677105649341495E-4
88-89	6.813511301444572E-4	0.002007875454319507	0.0	0.14756377175243632	1.5677105649341495E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27780	0.0	55.67226	1
GTATCAA	52635	0.0	38.433186	1
ATCAACG	68455	0.0	29.225332	3
TATCAAC	72265	0.0	27.824377	2
TCAACGC	72075	0.0	27.780851	4
CAACGCA	73700	0.0	27.139742	5
AACGCAG	74380	0.0	26.925598	6
ACGCAGA	74920	0.0	26.720205	7
GTGGTAT	11085	0.0	24.58312	1
TGGTATC	11185	0.0	23.045517	2
CGCAGAG	89535	0.0	22.362635	8
GTACATG	62890	0.0	20.794497	1
TACATGG	63240	0.0	20.266611	2
GCAGAGT	99000	0.0	20.109646	9
ACATGGG	63050	0.0	19.893475	3
AGAGTAC	91675	0.0	19.559736	10-11
GAGTACT	61190	0.0	19.300037	12-13
AGTACTT	65710	0.0	18.49162	12-13
CAGAGTA	96580	0.0	18.17606	10-11
GTACTTT	67770	0.0	17.427055	14-15
>>END_MODULE