FastQCFastQC Report
Fri 27 May 2016
SRR2139001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230007
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80900.054.4639851
CTTATAC112950.037.5784031
GTATCAA146450.036.2280731
TATACAC137600.032.4418263
TTATACA143100.030.12442
ATCAACG185100.028.2816643
TCAACGC187450.027.9271054
CAACGCA188950.027.6615335
TATCAAC190550.027.5608332
AACGCAG197750.026.554566
TGGTATC31900.024.8180332
GTGGTAT33700.024.7224121
TACACAT181250.024.3316565
ACATCTC179350.023.7757848
ACACATC180650.023.5160796
ACGCAGA225300.023.2123457
CGCAGAG226800.023.0588258
CACATCT189000.022.3864277
ATACACA205300.022.3292544
CATCTCC197150.021.587119
Produced by FastQC (version 0.11.5)