FastQCFastQC Report
Fri 27 May 2016
SRR2139000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346971
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.051.6098821
GTATCAA201900.036.8472981
ATCAACG245100.029.8697193
TCAACGC249750.029.2630754
CAACGCA250450.029.1309155
TATCAAC256500.028.741452
AACGCAG258800.028.2686126
ATCTCGT120250.024.66349894
ACGCAGA296250.024.5631667
CGCAGAG299550.024.3065998
GTGGTAT46000.023.312911
TGGTATC43850.023.2091872
TATCTCG142450.021.87591494
GCAGAGT345300.021.0861329
GTACATG249500.020.3446791
TACATGG247750.019.9112472
GAGTACT208850.019.54529412-13
ACATGGG254450.019.0712433
CAGAGTA343750.018.51534310-11
AGAGTAC322950.018.48424310-11
Produced by FastQC (version 0.11.5)