##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7346971
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49696657302717	34.0	31.0	34.0	31.0	34.0
2	32.624985861520344	34.0	31.0	34.0	31.0	34.0
3	32.67127051406627	34.0	31.0	34.0	31.0	34.0
4	36.18497446090368	37.0	35.0	37.0	35.0	37.0
5	36.102385595369846	37.0	35.0	37.0	35.0	37.0
6	36.11650964730907	37.0	36.0	37.0	35.0	37.0
7	36.07519738406481	37.0	36.0	37.0	35.0	37.0
8	36.073014035307885	37.0	36.0	37.0	35.0	37.0
9	37.811124747872285	39.0	38.0	39.0	35.0	39.0
10-11	37.74521261891465	39.0	38.0	39.0	35.0	39.0
12-13	37.72341145759252	39.0	38.0	39.0	35.0	39.0
14-15	39.158116793982174	41.0	39.0	41.0	36.0	41.0
16-17	39.1339025293553	41.0	39.0	41.0	36.0	41.0
18-19	39.14531233347729	41.0	39.0	41.0	36.0	41.0
20-21	39.13682352264935	41.0	39.0	41.0	35.5	41.0
22-23	39.121775132762025	41.0	39.0	41.0	35.0	41.0
24-25	39.077607063703454	41.0	39.0	41.0	35.0	41.0
26-27	39.01645721937913	41.0	39.0	41.0	35.0	41.0
28-29	38.96177145691992	41.0	39.0	41.0	35.0	41.0
30-31	38.8723209911423	40.5	38.0	41.0	35.0	41.0
32-33	38.77530466026623	40.0	38.0	41.0	35.0	41.0
34-35	38.69848042912096	40.0	38.0	41.0	34.0	41.0
36-37	38.67295637379728	40.0	38.0	41.0	34.0	41.0
38-39	38.626998414793114	40.0	38.0	41.0	34.0	41.0
40-41	38.62289778344007	40.0	38.0	41.0	34.0	41.0
42-43	38.57952423470542	40.0	38.0	41.0	34.0	41.0
44-45	38.49718649226493	40.0	38.0	41.0	34.0	41.0
46-47	38.45029905202759	40.0	38.0	41.0	33.5	41.0
48-49	38.40169499070106	40.0	38.0	41.0	33.0	41.0
50-51	38.34010920352408	40.0	38.0	41.0	33.0	41.0
52-53	38.22177350291243	40.0	37.5	41.0	33.0	41.0
54-55	38.122264180514634	40.0	37.0	41.0	33.0	41.0
56-57	38.00911462165626	40.0	37.0	41.0	33.0	41.0
58-59	37.91274211906132	40.0	37.0	41.0	33.0	41.0
60-61	37.79456550529227	40.0	36.0	41.0	33.0	41.0
62-63	37.607480669693786	39.0	36.0	41.0	32.0	41.0
64-65	37.42999780737614	39.0	35.5	41.0	32.0	41.0
66-67	37.21921400671122	39.0	35.0	41.0	32.0	41.0
68-69	36.90136129476892	38.5	35.0	41.0	31.5	41.0
70-71	36.586986809959974	37.5	35.0	40.0	31.0	41.0
72-73	36.295304078236825	37.0	35.0	39.5	31.0	41.0
74-75	36.01371803908151	36.5	35.0	39.0	31.0	41.0
76-77	35.70112034986518	36.0	35.0	39.0	31.0	41.0
78-79	35.39938736834562	36.0	35.0	38.0	31.0	39.5
80-81	35.08002432617286	35.0	34.0	37.0	31.0	39.0
82-83	34.749873251311044	35.0	34.0	37.0	31.0	39.0
84-85	34.51450139933576	35.0	34.0	36.0	31.0	38.0
86-87	34.32916188662814	35.0	34.0	36.0	31.0	37.0
88-89	34.16792684142192	35.0	34.0	36.0	31.0	37.0
90-91	34.02876589007607	35.0	34.0	35.0	31.0	36.5
92-93	33.963335631364174	35.0	34.0	35.0	31.0	36.0
94-95	33.876988803838294	35.0	34.0	35.0	31.0	36.0
96-97	33.85749874849904	35.0	34.0	35.0	31.0	36.0
98-99	34.005581811074904	35.0	34.0	35.0	31.0	36.0
100	31.154133157450364	33.0	29.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	15.0
19	39.0
20	80.0
21	238.0
22	867.0
23	2850.0
24	7305.0
25	15855.0
26	28818.0
27	47330.0
28	68741.0
29	93904.0
30	121167.0
31	152384.0
32	191062.0
33	239098.0
34	312969.0
35	434073.0
36	631075.0
37	1186910.0
38	2038613.0
39	1608713.0
40	164864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0573379151762	21.286255247230457	14.773761867305588	23.882644970287757
2	14.295503298269132	21.58192776381559	41.9724059131314	22.15016302478388
3	17.397495018347726	27.137904657164324	31.278909916482462	24.185690408005488
4	12.023431152783916	19.140119104866482	39.4232670851702	29.413182657179398
5	12.386542116530117	38.499966921304555	36.251010397600474	12.862480564564848
6	28.327357973406208	39.19238508135243	19.28118441584748	13.199072529393884
7	25.123128973831527	33.465954881270115	23.914549274796375	17.49636687010198
8	23.627478589475853	37.26866486882825	21.80131104369406	17.302545498001827
9	25.131472548346796	16.81257486928967	21.86325493866792	36.19269764369562
10-11	23.422796333697722	27.382328475296575	29.563548438665126	19.631326752340573
12-13	24.372042105251694	25.01587899609492	29.552729189906124	21.05934970874726
14-15	21.505018871042232	26.35217288866391	27.386830572762573	24.755977667531287
16-17	20.844580921418295	29.425861400496302	28.15288339895811	21.576674279127293
18-19	20.891584595859918	28.39029017377106	30.035894011467136	20.682231218901883
20-21	21.8512017586546	27.489261929960087	29.86715487934772	20.792381432037597
22-23	21.51454854661563	27.660624035801085	29.631374467767646	21.19345294981564
24-25	21.335956562034376	27.719019306821618	29.79570571578984	21.149318415354166
26-27	21.294197979373028	27.86923280860828	29.71708960834038	21.119479603678315
28-29	21.153086799507946	27.80501559413845	29.846740583728266	21.19515702262534
30-31	21.31202891677903	27.523860112458998	29.920934779900744	21.24317619086123
32-33	21.058590016102134	27.842294337066058	29.709541532861923	21.389574113969882
34-35	21.356956567871983	27.834556253766507	29.692917309642024	21.11556986871949
36-37	21.241011797493776	27.839214454597943	29.704072252503384	21.215701495404904
38-39	21.094750384757173	27.746875782895614	29.7233404911366	21.43503334121061
40-41	21.47461605458394	27.587158504110356	29.729112006717447	21.209113434588264
42-43	21.39510242015953	27.606580800500318	29.50151491274631	21.49680186659385
44-45	21.336836384102607	27.955709815162884	29.202157132754902	21.505296667979607
46-47	21.51145860137598	27.774479410197817	28.964107276622038	21.74995471180417
48-49	21.48032653445163	27.974796150397612	28.83085349253271	21.714023822618046
50-51	21.285509237310045	28.105219486612437	28.659040546915328	21.950230729162193
52-53	21.245416944621823	27.99067423145601	28.754051729717684	22.009857094204484
54-55	21.51134410227713	27.742349245877683	28.901955687712245	21.844350964132946
56-57	21.481743258940487	27.456030085188683	28.802773217382487	22.259453438488343
58-59	21.002230652500394	27.440781967253148	29.177518672968244	22.379468707278217
60-61	21.447839227676898	27.92678998015062	29.062259347502167	21.563111444670316
62-63	20.761451602994907	28.26692849052572	29.000948269056902	21.97067163742247
64-65	20.52069710922023	28.577576407846017	28.74672109433864	22.155005388595118
66-67	20.71036089861685	29.218970814036304	28.16941616846758	21.90125211887926
68-69	20.88784249895566	29.063066178230844	27.966739374953054	22.08235194786044
70-71	20.87485922386974	28.34215658467344	28.021812857222432	22.761171334234387
72-73	21.18946012629195	27.70292922079995	28.038921357650636	23.068689295257467
74-75	21.36903577575766	27.71047836386623	28.238733191444865	22.681752668931242
76-77	21.656044352825017	27.678089406561867	27.465203608584694	23.200662632028422
78-79	21.928837559797064	27.721118464829146	26.98854541218254	23.361498563191248
80-81	21.678645547914336	28.162004546634297	27.242821430478774	22.91652847497259
82-83	21.696496452171623	27.91706959239925	27.313441257843323	23.072992697585807
84-85	21.801177727558503	28.349157291558154	27.198711817950063	22.650953162933288
86-87	22.30002332264806	28.25531739577791	27.28519071967256	22.159468561901473
88-89	21.994961640250068	28.38918280516274	27.718806230092508	21.897049324494684
90-91	21.926817069069173	27.927478802017603	27.598860581423875	22.546843547489352
92-93	21.984370338158048	27.42383411523569	27.817396465837096	22.77439908076916
94-95	21.075752468367423	28.029091237789984	28.58334635860487	22.311809935237722
96-97	21.17644072579758	27.903418086413627	28.791285132391543	22.128856055397254
98-99	22.30739427004173	29.686249105907454	29.18986462415012	18.8164919999007
100	21.439524524774185	30.908383816549627	22.41824305474727	25.233848603928916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2079.0
1	1711.5
2	2060.5
3	2823.5
4	3598.5
5	6070.0
6	8215.0
7	9635.0
8	10446.5
9	9941.5
10	8488.0
11	6781.5
12	7020.5
13	9787.5
14	17416.0
15	28770.0
16	38224.0
17	43481.0
18	44957.5
19	43330.0
20	39635.0
21	35891.5
22	34286.5
23	36990.5
24	44419.5
25	55368.5
26	70054.0
27	87508.0
28	104946.0
29	123722.5
30	141916.0
31	158502.0
32	176389.5
33	194043.5
34	212654.5
35	230777.0
36	247179.5
37	262297.0
38	271832.0
39	278290.0
40	287178.5
41	295079.5
42	299375.5
43	304213.5
44	309418.0
45	316285.5
46	322785.0
47	322343.5
48	316050.5
49	305848.5
50	293796.0
51	279361.5
52	263094.5
53	245281.0
54	225836.0
55	205455.0
56	183164.0
57	161355.5
58	140621.0
59	121258.0
60	101561.5
61	81572.5
62	64179.5
63	49875.0
64	37815.0
65	28240.5
66	21264.5
67	16263.0
68	12635.5
69	9285.5
70	6804.0
71	5146.0
72	3956.5
73	3093.5
74	2439.0
75	1910.0
76	1449.0
77	1082.5
78	794.0
79	562.0
80	406.0
81	294.0
82	225.5
83	165.5
84	115.5
85	86.0
86	68.5
87	55.5
88	35.5
89	19.0
90	12.0
91	9.0
92	8.0
93	7.5
94	8.0
95	8.0
96	6.5
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007377189865047787
3	1.497215655268001E-4
4	0.0
5	0.011610226854032771
6	0.013202719869181463
7	0.0
8	0.0
9	0.0
10-11	6.124973135187278E-5
12-13	2.0416577117290923E-5
14-15	0.0
16-17	3.4708181099394567E-4
18-19	8.574962389262188E-4
20-21	0.0
22-23	1.089104543689701E-4
24-25	0.0
26-27	6.811897687749013E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.3762067613038183E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.953352159845523E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.006277547551682428
52-53	8.174234248882248E-5
54-55	2.9773951692656597E-5
56-57	0.0
58-59	7.4676185391097855E-6
60-61	7.530680746429026E-5
62-63	0.0011418377970690851
64-65	0.0014780983925449028
66-67	0.0011771412882150929
68-69	0.009879684241816762
70-71	8.163188214789169E-4
72-73	4.941989549393443E-4
74-75	4.3431711623268416E-4
76-77	1.409782814664561E-4
78-79	0.0
80-81	1.3606548287428807E-4
82-83	0.0
84-85	2.6174043253653436E-5
86-87	1.76820370131008E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	264.0
20-21	879.0
22-23	1633.0
24-25	3073.0
26-27	5035.0
28-29	9236.0
30-31	22659.0
32-33	29138.0
34-35	38599.0
36-37	54214.0
38-39	37045.0
40-41	62804.0
42-43	71440.0
44-45	83608.0
46-47	83303.0
48-49	73186.0
50-51	39457.0
52-53	11309.0
54-55	11113.0
56-57	10732.0
58-59	40590.0
60-61	71377.0
62-63	72205.0
64-65	79478.0
66-67	82940.0
68-69	83768.0
70-71	78789.0
72-73	70030.0
74-75	71344.0
76-77	74105.0
78-79	74879.0
80-81	76526.0
82-83	73071.0
84-85	74484.0
86-87	78035.0
88-89	82225.0
90-91	85659.0
92-93	92196.0
94-95	112148.0
96-97	279072.0
98-99	615171.0
100-101	4330152.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.07356820389896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.8535182361787	72.98305239145674
2	8.291943129899447	13.776826062891065
3	2.1908003425411082	5.459928050316418
4	0.8372049388275534	2.781984063453273
5	0.35417908376076307	1.4711460135597096
6	0.17097535957359872	0.8522119916834103
7	0.10231851489321861	0.5949974887852414
8	0.0647677036804321	0.43043873992850357
9	0.03695926545496704	0.27633042535852903
>10	0.09534654982269233	1.1207820454448145
>50	0.0012468101490743897	0.06638543598384346
>100	6.372938487360684E-4	0.11565304920457588
>500	7.707860600762805E-5	0.04452350387494105
>1k	2.5692763694580303E-5	0.025740738058968215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.3611051411527281E-5	1.3611051411527281E-5	0.0	0.0	0.0
3	1.3611051411527281E-5	1.3611051411527281E-5	0.0	0.0	0.0
4	1.3611051411527281E-5	2.7222102823054563E-5	0.0	2.7222102823054563E-5	0.0
5	1.3611051411527281E-5	2.7222102823054563E-5	0.0	2.7222102823054563E-5	0.0
6	1.3611051411527281E-5	2.7222102823054563E-5	0.0	2.7222102823054563E-5	0.0
7	2.7222102823054563E-5	4.0833154234581846E-5	0.0	4.0833154234581846E-5	0.0
8	2.7222102823054563E-5	4.0833154234581846E-5	0.0	4.0833154234581846E-5	0.0
9	4.0833154234581846E-5	6.80552570576364E-5	0.0	4.0833154234581846E-5	0.0
10-11	4.0833154234581846E-5	6.80552570576364E-5	0.0	5.4444205646109126E-5	0.0
12-13	4.0833154234581846E-5	6.80552570576364E-5	0.0	5.4444205646109126E-5	0.0
14-15	4.0833154234581846E-5	9.527735988069096E-5	0.0	6.124973135187276E-5	0.0
16-17	5.4444205646109126E-5	1.0208288558645461E-4	0.0	6.80552570576364E-5	0.0
18-19	5.4444205646109126E-5	1.0888841129221825E-4	0.0	6.80552570576364E-5	0.0
20-21	5.4444205646109126E-5	1.2249946270374552E-4	0.0	8.166630846916369E-5	0.0
22-23	5.4444205646109126E-5	1.2930498840950917E-4	0.0	8.847183417492734E-5	0.0
24-25	8.166630846916369E-5	1.361105141152728E-4	0.0	1.6333261693832738E-4	0.0
26-27	9.527735988069096E-5	1.361105141152728E-4	0.0	3.6069286240547296E-4	0.0
28-29	9.527735988069096E-5	1.361105141152728E-4	0.0	0.0012658277812720373	0.0
30-31	1.0208288558645461E-4	1.497215655268001E-4	0.0	0.0055260868730800765	0.0
32-33	1.0888841129221825E-4	1.497215655268001E-4	0.0	0.014591047113157245	0.0
34-35	1.2249946270374552E-4	1.497215655268001E-4	0.0	0.027249324925877616	0.0
36-37	1.2249946270374552E-4	1.497215655268001E-4	0.0	0.05123199751298869	0.0
38-39	1.361105141152728E-4	1.5652709123256374E-4	0.0	0.2554930460457786	0.0
40-41	1.361105141152728E-4	1.7694366834985464E-4	0.0	0.4672742004834373	0.0
42-43	1.361105141152728E-4	1.837491940556183E-4	0.0	0.5167843999928678	0.0
44-45	1.361105141152728E-4	2.2458234829020015E-4	0.0	0.5898213018671232	0.0
46-47	2.0416577117290922E-4	2.5860997681901834E-4	0.0	0.6672341567701846	0.0
48-49	2.177768225844365E-4	2.5860997681901834E-4	0.0	0.7254962623372272	0.0
50-51	2.177768225844365E-4	2.926376053478365E-4	0.0	0.7817507378210694	0.0
52-53	2.313878739959638E-4	2.994431310536002E-4	0.0	1.3660187307122893	0.0
54-55	2.313878739959638E-4	3.0624865675936387E-4	0.0	2.1435908757500197	0.0
56-57	2.722210282305456E-4	3.2666523387665477E-4	0.0	2.9808134535987687	0.0
58-59	3.130541824651275E-4	3.2666523387665477E-4	0.0	3.8336411018908336	0.0
60-61	3.130541824651275E-4	3.40276285288182E-4	0.0	4.060802744423518	0.0
62-63	3.4027628528818205E-4	3.538873366997093E-4	0.0	4.069200763144431	0.0
64-65	3.4027628528818205E-4	4.0833154234581843E-4	0.0	4.075815734130433	0.0
66-67	3.6069286240547296E-4	4.1513706805158205E-4	0.0	4.08185904095715	0.0
68-69	3.879149652285275E-4	4.287481194631094E-4	0.0	4.087834292526811	0.0
70-71	3.9472049093429115E-4	4.35553645168873E-4	0.0	4.092931631280429	0.0
72-73	3.9472049093429115E-4	4.35553645168873E-4	0.0	4.097205501423648	0.0
74-75	3.9472049093429115E-4	4.5597022228616396E-4	0.0	4.100764791367762	0.0
76-77	3.9472049093429115E-4	4.7638679940345487E-4	0.0	4.10404505475794	0.0
78-79	3.9472049093429115E-4	4.7638679940345487E-4	0.0	4.106318100343666	0.0
80-81	4.0833154234581843E-4	4.7638679940345487E-4	0.0	4.107100735799828	0.0
82-83	4.219425937573457E-4	4.831923251092185E-4	0.0	4.107822121524639	0.0
84-85	4.219425937573457E-4	4.899978508149821E-4	0.0	4.108107953604281	0.0
86-87	4.627757479919276E-4	4.968033765207458E-4	0.0	4.108237258592691	0.0
88	4.627757479919276E-4	5.036089022265094E-4	0.0	4.1082508696441025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10805	0.0	51.609882	1
GTATCAA	20190	0.0	36.847298	1
ATCAACG	24510	0.0	29.869719	3
TCAACGC	24975	0.0	29.263075	4
CAACGCA	25045	0.0	29.130915	5
TATCAAC	25650	0.0	28.74145	2
AACGCAG	25880	0.0	28.268612	6
ATCTCGT	12025	0.0	24.663498	94
ACGCAGA	29625	0.0	24.563166	7
CGCAGAG	29955	0.0	24.306599	8
GTGGTAT	4600	0.0	23.31291	1
TGGTATC	4385	0.0	23.209187	2
TATCTCG	14245	0.0	21.875914	94
GCAGAGT	34530	0.0	21.086132	9
GTACATG	24950	0.0	20.344679	1
TACATGG	24775	0.0	19.911247	2
GAGTACT	20885	0.0	19.545294	12-13
ACATGGG	25445	0.0	19.071243	3
CAGAGTA	34375	0.0	18.515343	10-11
AGAGTAC	32295	0.0	18.484243	10-11
>>END_MODULE