FastQCFastQC Report
Fri 27 May 2016
SRR2138999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723432
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.046.6146741
CTTATAC62000.038.8094021
GTATCAA135100.036.1065061
ATCAACG149250.032.2436563
TCAACGC151150.031.8094024
CAACGCA154300.031.1883745
AACGCAG159600.030.206376
TATCAAC167650.029.0179822
ACGCAGA175500.027.4447987
TATACAC102400.026.9157563
CGCAGAG180000.026.8072838
TTATACA101350.025.1226372
ACATCTC103350.023.4080098
ACACATC108000.022.2786456
GCAGAGT220150.021.7990599
TACACAT131100.020.222555
TACATGG157800.019.9614222
GTACATG164300.019.8107681
CACATCT120250.019.7544337
CATCTCC119850.019.7473589
Produced by FastQC (version 0.11.5)