##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6723432
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.41303622911632	32.0	28.0	33.0	18.0	34.0
2	31.560788894719245	33.0	31.0	33.0	27.0	34.0
3	32.350032096702996	33.0	33.0	34.0	30.0	34.0
4	32.622821053295404	33.0	33.0	34.0	31.0	34.0
5	32.960988227440986	33.0	33.0	34.0	32.0	34.0
6	36.54596952865739	38.0	37.0	38.0	34.0	38.0
7	36.663271823080834	38.0	37.0	38.0	34.0	38.0
8	36.71481365469302	38.0	38.0	38.0	34.0	38.0
9	36.93923163051251	38.0	38.0	38.0	35.0	38.0
10-11	37.06662341494641	38.0	38.0	38.0	36.0	38.0
12-13	37.12549818307079	38.0	38.0	38.0	36.0	38.0
14-15	37.125624234765816	38.0	38.0	38.0	36.0	38.0
16-17	37.084844391971245	38.0	38.0	38.0	36.0	38.0
18-19	37.127772467989566	38.0	38.0	38.0	36.0	38.0
20-21	37.09825439418441	38.0	38.0	38.0	36.0	38.0
22-23	37.13953814225583	38.0	38.0	38.0	36.0	38.0
24-25	37.14560414500563	38.0	38.0	38.0	36.0	38.0
26-27	37.07838313144323	38.0	38.0	38.0	36.0	38.0
28-29	37.06750365728297	38.0	38.0	38.0	36.0	38.0
30-31	36.93810575516337	38.0	38.0	38.0	36.0	38.0
32-33	37.02988110358705	38.0	38.0	38.0	36.0	38.0
34-35	37.04263198469078	38.0	38.0	38.0	36.0	38.0
36-37	36.96769718196113	38.0	38.0	38.0	36.0	38.0
38-39	36.98562091532918	38.0	38.0	38.0	36.0	38.0
40-41	36.92615015528172	38.0	38.0	38.0	35.5	38.0
42-43	36.91093608180462	38.0	38.0	38.0	35.5	38.0
44-45	36.90989207283164	38.0	38.0	38.0	36.0	38.0
46-47	36.86829257375117	38.0	38.0	38.0	35.0	38.0
48-49	36.83472384972754	38.0	38.0	38.0	35.0	38.0
50-51	36.853674020557754	38.0	38.0	38.0	35.0	38.0
52-53	36.763120167588724	38.0	38.0	38.0	35.0	38.0
54-55	36.747338539761756	38.0	38.0	38.0	35.0	38.0
56-57	36.74953359959636	38.0	38.0	38.0	35.0	38.0
58-59	36.76887185278177	38.0	38.0	38.0	35.0	38.0
60-61	36.76065597175624	38.0	38.0	38.0	35.0	38.0
62-63	36.76405173905111	38.0	38.0	38.0	35.0	38.0
64-65	36.722230614788	38.0	38.0	38.0	35.0	38.0
66-67	36.722958459622646	38.0	38.0	38.0	35.0	38.0
68-69	36.73344092905089	38.0	38.0	38.0	35.0	38.0
70-71	36.64672806589644	38.0	38.0	38.0	34.0	38.0
72-73	36.60147890144763	38.0	38.0	38.0	34.0	38.0
74-75	36.65306755737201	38.0	38.0	38.0	34.0	38.0
76-77	36.63068319572424	38.0	38.0	38.0	34.0	38.0
78-79	36.64930657144697	38.0	38.0	38.0	34.0	38.0
80-81	36.671709906103644	38.0	38.0	38.0	34.0	38.0
82-83	36.546151770861655	38.0	38.0	38.0	34.0	38.0
84-85	36.56471337651436	38.0	38.0	38.0	34.0	38.0
86-87	36.560962569802406	38.0	38.0	38.0	34.0	38.0
88-89	36.5108185292521	38.0	38.0	38.0	34.0	38.0
90-91	36.51016234455095	38.0	38.0	38.0	34.0	38.0
92-93	36.55837947524048	38.0	38.0	38.0	34.0	38.0
94-95	36.45949544914559	38.0	38.0	38.0	34.0	38.0
96-97	36.41913640877736	38.0	38.0	38.0	34.0	38.0
98-99	36.48880425754352	38.0	38.0	38.0	34.0	38.0
100	34.85984317370247	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	22.0
20	53.0
21	177.0
22	658.0
23	2193.0
24	5271.0
25	10375.0
26	17990.0
27	28778.0
28	42479.0
29	59506.0
30	79944.0
31	103594.0
32	134050.0
33	178580.0
34	255569.0
35	427987.0
36	1071911.0
37	4304292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.272730355568406	20.181285391151423	15.369353032796345	26.176631220483827
2	12.711186786748197	21.088218041024287	44.438807442389546	21.761787729837977
3	15.941441811265436	28.117857070615127	31.801526363321592	24.13917475479785
4	11.109832002465406	20.195563813242998	40.72789016085832	27.96671402343327
5	12.50131634450684	37.9315750738789	37.08054719474343	12.486561386870825
6	27.12609083070831	40.28394133887892	20.234802736876457	12.355165093536321
7	24.226972177304688	33.58165889087597	25.342711876910485	16.848657054908863
8	22.706870538736766	37.82532492334273	22.742878934448953	16.72492560347156
9	24.55368397474444	17.343168986191724	22.658996574814257	35.44415046424958
10-11	22.47193843858315	27.83926423290962	30.595066626687085	19.093730701820142
12-13	23.556845670484954	25.309031161466343	30.489555036772884	20.64456813127581
14-15	20.96096160413313	26.708606556889396	27.95443606777015	24.37599577120732
16-17	20.55628732468775	29.84764328694036	28.622227457643657	20.973841930728234
18-19	20.517348877775518	29.039016085832355	30.22773934502498	20.215895691367148
20-21	21.312438897675015	28.182768330028185	29.952792899614227	20.551999872682575
22-23	20.95183592846524	28.31902831478924	29.78461037323939	20.944525383506132
24-25	20.874059712801767	28.395152445858603	29.935831920358336	20.79495592098129
26-27	20.8144392843789	28.53948558401885	29.88131419498354	20.764760936618714
28-29	20.575839316517307	28.441905764024778	30.114219044680844	20.868035874777068
30-31	20.980324935032492	28.213031490911394	29.80448044677934	21.002163127276766
32-33	21.017131495359166	28.25855479422862	30.00068254157021	20.723631168842008
34-35	21.02489512911004	28.594950226906203	29.81671381907654	20.563440824907218
36-37	20.849101506780485	28.450506218490613	29.766120003408027	20.93427227132087
38-39	20.725347059125138	28.451450008719505	30.000195935457807	20.82300699669755
40-41	20.971664911724353	28.32588453351462	29.89570675204161	20.806743802719406
42-43	21.033774415565095	28.302172177639594	29.777702579685688	20.886350827109617
44-45	20.87723502064296	28.595952340967184	29.653427936528182	20.873384701861674
46-47	21.117039504166804	28.330181141789517	29.307327344781875	21.245452009261808
48-49	21.084102407751317	28.343252364225492	29.39213201224747	21.180513215775722
50-51	20.93251408832729	28.47218057974693	29.439022013103394	21.156283318822382
52-53	21.063609338068876	28.581353412012767	29.47692785549931	20.87810939441905
54-55	21.038752371386774	28.515693948466847	29.341092379815937	21.10446130033045
56-57	21.274301603497587	28.649032465314033	28.978516760594413	21.098149170593967
58-59	20.902684783190235	28.931710323208343	29.415870914815745	20.749733978785674
60-61	21.170975632752516	29.219119847471582	28.95057423898916	20.659330280786744
62-63	20.9499575503147	29.38301130912934	28.931373637769802	20.73565750278615
64-65	20.92123475132463	29.24039027360528	28.940309778400913	20.898065196669176
66-67	21.04250092676707	29.21525030858288	28.82111969756841	20.92112906708164
68-69	21.043681938667937	29.08291159456411	28.944784906037448	20.928621560730505
70-71	21.115582221886072	28.688326370408994	29.04617556653789	21.14991584116704
72-73	21.148042324383287	28.553654011621028	29.155761992364894	21.14254167163079
74-75	21.117963819040053	28.66468210686014	29.025667031586643	21.191687042513163
76-77	21.094564774274783	28.676223301047578	28.972684598530208	21.256527326147438
78-79	21.12717611015833	28.66032531430431	28.974815842986928	21.23768273255043
80-81	21.116049347768133	28.66315402376147	28.96301834475758	21.257778283712813
82-83	21.19873823072678	28.58686765699622	29.054232466467994	21.16016164580901
84-85	21.345623351408456	28.608321523258912	28.878014725539952	21.168040399792684
86-87	21.1249882224666	28.66822215548041	28.96351342249906	21.24327619955393
88-89	21.288650310357703	28.598805134441985	28.895943796536272	21.216600758664043
90-91	21.291393351848136	28.647739460920945	28.926063970752814	21.134803216478108
92-93	21.292911188917664	28.66546635749722	28.89869546682753	21.142926986757583
94-95	21.18096040455059	28.74721002515615	28.856230098683323	21.215599471609934
96-97	21.23272043478346	28.87059519150404	28.963366889574633	20.93331748413787
98-99	22.18059676392509	30.737816670470515	29.3229247998975	17.758661765706897
100	21.028358918933527	31.796395642780272	23.32596404425965	23.849281394026548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	629.0
1	717.5
2	1315.5
3	2025.5
4	2725.5
5	3955.0
6	4924.0
7	5665.0
8	6397.5
9	6759.0
10	6778.5
11	6957.5
12	7900.5
13	9839.0
14	13489.0
15	17766.0
16	21154.5
17	23533.0
18	24073.5
19	23116.0
20	22271.5
21	22348.0
22	24611.0
23	29891.0
24	38318.5
25	50029.5
26	65626.0
27	83487.0
28	101261.5
29	120052.0
30	139401.5
31	158436.0
32	177375.5
33	196401.0
34	216683.0
35	235301.5
36	251814.5
37	266667.5
38	275669.5
39	281308.0
40	287655.0
41	290699.0
42	290043.0
43	290162.0
44	293124.0
45	300002.5
46	294103.0
47	281505.0
48	269405.5
49	251236.5
50	235435.5
51	217215.0
52	196731.5
53	177151.5
54	156579.0
55	136715.5
56	119578.5
57	103285.0
58	87141.0
59	72568.5
60	58615.5
61	45761.5
62	35007.0
63	26221.0
64	19315.5
65	14218.0
66	10737.5
67	7721.5
68	5234.5
69	3827.0
70	2809.5
71	1784.0
72	1161.5
73	738.0
74	442.5
75	272.0
76	186.0
77	108.5
78	58.0
79	35.5
80	20.5
81	15.0
82	14.0
83	10.5
84	6.5
85	3.0
86	2.5
87	2.5
88	2.0
89	2.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0039860595005645925
6	1.4873356345390271E-5
7	0.0
8	0.0
9	1.4873356345390271E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.4874723590448881E-5
26-27	0.0
28-29	4.463626465882829E-5
30-31	0.0
32-33	2.9805299859199764E-5
34-35	0.0
36-37	5.31098907970882E-4
38-39	4.504261369074747E-5
40-41	1.8844645346789718E-4
42-43	6.813245312260117E-5
44-45	7.457083533108348E-4
46-47	0.0
48-49	7.702998862190038E-5
50-51	0.0
52-53	1.246166771539389E-4
54-55	0.0022474125586196846
56-57	0.0012130912076803483
58-59	1.505965127871499E-4
60-61	3.66929622260312E-4
62-63	8.832802910135545E-5
64-65	7.437484907766699E-4
66-67	0.001328009021988163
68-69	3.28695899839128E-4
70-71	7.21498387948688E-4
72-73	1.1721316327266185E-4
74-75	1.349159309321502E-4
76-77	8.494835734511918E-6
78-79	0.001720883928176983
80-81	0.0
82-83	9.831103383883186E-4
84-85	1.4905967893597344E-4
86-87	0.0
88-89	4.4568551022032646E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.066977205853651E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	144.0
22-23	333.0
24-25	472.0
26-27	999.0
28-29	2244.0
30-31	6882.0
32-33	9439.0
34-35	13636.0
36-37	22096.0
38-39	27206.0
40-41	27736.0
42-43	31990.0
44-45	39048.0
46-47	40468.0
48-49	38140.0
50-51	34544.0
52-53	33845.0
54-55	36950.0
56-57	39238.0
58-59	39377.0
60-61	41496.0
62-63	41434.0
64-65	46481.0
66-67	51212.0
68-69	52072.0
70-71	62868.0
72-73	42858.0
74-75	43061.0
76-77	45357.0
78-79	46538.0
80-81	46611.0
82-83	45158.0
84-85	44833.0
86-87	47203.0
88-89	48723.0
90-91	50009.0
92-93	50824.0
94-95	61824.0
96-97	202575.0
98-99	471136.0
100-101	4736323.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.92705111084227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.38713696454785	78.57087274679951
2	6.955515427132399	12.092448900731798
3	1.5971656196289856	4.165106923499066
4	0.5525404207209631	1.921228375712698
5	0.21872136043817025	0.9506401438920891
6	0.10465672243791636	0.5458500156272394
7	0.0635107827776529	0.3864563542421844
8	0.03877201560551242	0.26962695857686014
9	0.02280277224152574	0.17839599732972028
>10	0.057375707949702794	0.7051028683992969
>50	0.001118785718595961	0.0660968048529616
>100	6.346748502277295E-4	0.11097004990605443
>500	4.874595056047193E-5	0.03720386043055055
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9746712690780543E-5	0.0	2.9746712690780543E-5	0.0
3	0.0	2.9746712690780543E-5	0.0	2.9746712690780543E-5	0.0
4	0.0	4.462006903617081E-5	0.0	2.9746712690780543E-5	0.0
5	0.0	5.9493425381561086E-5	0.0	4.462006903617081E-5	0.0
6	0.0	5.9493425381561086E-5	0.0	4.462006903617081E-5	0.0
7	0.0	7.436678172695136E-5	0.0	4.462006903617081E-5	0.0
8	0.0	1.0411349441773189E-4	0.0	4.462006903617081E-5	1.4873356345390271E-5
9	0.0	2.3797370152624434E-4	0.0	4.462006903617081E-5	1.4873356345390271E-5
10-11	0.0	2.454103796989395E-4	0.0	5.9493425381561086E-5	1.4873356345390271E-5
12-13	0.0	2.6028373604432975E-4	0.0	6.693010355425622E-5	1.4873356345390271E-5
14-15	0.0	3.569605522893665E-4	0.0	8.924013807234162E-5	1.4873356345390271E-5
16-17	0.0	3.7183390863475675E-4	0.0	9.667681624503675E-5	1.4873356345390271E-5
18-19	0.0	4.238906558436227E-4	0.0	1.3386020710851244E-4	1.4873356345390271E-5
20-21	0.0	4.610740467070984E-4	0.0	1.4129688528120756E-4	1.4873356345390271E-5
22-23	0.0	4.833840812251838E-4	0.0	1.6360691979929297E-4	1.4873356345390271E-5
24-25	0.0	5.428775066067449E-4	0.0	1.7848027614468325E-4	1.4873356345390271E-5
26-27	0.0	5.874975756429157E-4	0.0	1.7848027614468325E-4	1.4873356345390271E-5
28-29	0.0	6.02370931988306E-4	0.0	2.6028373604432975E-4	1.4873356345390271E-5
30-31	0.0	6.395543228517816E-4	0.0	5.428775066067448E-4	1.4873356345390271E-5
32-33	0.0	6.395543228517816E-4	0.0	0.001435278887330161	1.4873356345390271E-5
34-35	0.0	7.362311390968184E-4	0.0	0.002565653969579822	2.9746712690780543E-5
36-37	0.0	8.403446335145503E-4	0.0	0.0043950768000628245	2.9746712690780543E-5
38-39	0.0	8.700913462053308E-4	0.0	0.0075407916671128665	2.9746712690780543E-5
40-41	0.0	8.998380588961113E-4	0.0	0.011155017259042704	2.9746712690780543E-5
42-43	0.0	9.072747370688065E-4	0.0	0.014345352195128917	2.9746712690780543E-5
44-45	0.0	0.0010113882314865385	0.0	0.01805625460330379	2.9746712690780543E-5
46-47	0.0	0.0011155017259042704	0.0	0.02228772448356732	2.9746712690780543E-5
48-49	0.0	0.0011155017259042704	0.0	0.026742294709011707	4.462006903617081E-5
50-51	1.4873356345390271E-5	0.0012493619330127827	0.0	0.03138278188877347	4.462006903617081E-5
52-53	1.4873356345390271E-5	0.0012493619330127827	0.0	0.03553988498731005	5.205674720886595E-5
54-55	1.4873356345390271E-5	0.0012493619330127827	0.0	0.04044809258128884	5.9493425381561086E-5
56-57	1.4873356345390271E-5	0.0012493619330127827	0.0	0.045267060037195286	6.693010355425622E-5
58-59	1.4873356345390271E-5	0.0012493619330127827	0.0	0.05036862126366415	7.436678172695136E-5
60-61	1.4873356345390271E-5	0.0012716719675308682	0.0	0.05517271536322521	7.436678172695136E-5
62-63	1.4873356345390271E-5	0.0012939820020489536	0.0	0.059865259290195844	1.0411349441773189E-4
64-65	1.4873356345390271E-5	0.0014724622781936369	0.0	0.06422315269939519	1.0411349441773189E-4
66-67	2.9746712690780543E-5	0.001487335634539027	0.0	0.06870003295935767	1.0411349441773189E-4
68-69	2.9746712690780543E-5	0.0015319557035751979	0.0	0.07328846339191056	1.0411349441773189E-4
70-71	2.9746712690780543E-5	0.0015617024162659786	0.0	0.07749018655948331	1.0411349441773189E-4
72-73	2.9746712690780543E-5	0.0015914491289567589	0.0	0.08108953879506775	1.1898685076312217E-4
74-75	2.9746712690780543E-5	0.0016732525888564055	0.0	0.08360313601743871	1.1898685076312217E-4
76-77	2.9746712690780543E-5	0.0017699294051014424	0.0	0.08597543635452846	1.1898685076312217E-4
78-79	2.9746712690780543E-5	0.0017996761177922229	0.0	0.08748508202358557	1.1898685076312217E-4
80-81	2.9746712690780543E-5	0.0017996761177922229	0.0	0.08798333946115615	1.1898685076312217E-4
82-83	2.9746712690780543E-5	0.0018219861523103083	0.0	0.08845185018603594	1.3386020710851244E-4
84-85	2.9746712690780543E-5	0.0018591695431737839	0.0	0.08879393738197991	1.4129688528120756E-4
86-87	2.9746712690780543E-5	0.0018889162558645644	0.0	0.08886086748553418	1.4873356345390272E-4
88	2.9746712690780543E-5	0.0020525231756638573	0.0	0.08886830416370686	1.6360691979929297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7480	0.0	46.614674	1
CTTATAC	6200	0.0	38.809402	1
GTATCAA	13510	0.0	36.106506	1
ATCAACG	14925	0.0	32.243656	3
TCAACGC	15115	0.0	31.809402	4
CAACGCA	15430	0.0	31.188374	5
AACGCAG	15960	0.0	30.20637	6
TATCAAC	16765	0.0	29.017982	2
ACGCAGA	17550	0.0	27.444798	7
TATACAC	10240	0.0	26.915756	3
CGCAGAG	18000	0.0	26.807283	8
TTATACA	10135	0.0	25.122637	2
ACATCTC	10335	0.0	23.408009	8
ACACATC	10800	0.0	22.278645	6
GCAGAGT	22015	0.0	21.799059	9
TACACAT	13110	0.0	20.22255	5
TACATGG	15780	0.0	19.961422	2
GTACATG	16430	0.0	19.810768	1
CACATCT	12025	0.0	19.754433	7
CATCTCC	11985	0.0	19.747358	9
>>END_MODULE