FastQCFastQC Report
Fri 27 May 2016
SRR2138998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8502258
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.050.9068371
GTATCAA171750.038.2468531
ATCAACG205250.031.1513753
TCAACGC206250.031.0005194
CAACGCA212650.030.0668055
TATCAAC218900.029.5500622
AACGCAG224400.028.5496236
ACGCAGA248200.025.7944077
CGCAGAG261750.024.4424348
GTACATG242900.020.754411
GCAGAGT309300.020.6565789
TACATGG234550.020.5256062
ACATGGG232850.020.2990153
GAGTACT176700.020.0052112-13
CAGAGTA291100.019.35441210-11
CATGGGG175500.019.1486844
AGAGTAC274300.018.63856110-11
GTACTTT201900.017.35717214-15
GTATAAT63050.016.6835291
GTGGTAT53050.016.6196521
Produced by FastQC (version 0.11.5)