FastQCFastQC Report
Fri 27 May 2016
SRR2138997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8620372
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94250.048.3680571
GTATCAA166150.035.0965231
TCAACGC183200.030.8766254
ATCAACG184350.030.589283
CAACGCA186900.030.2180395
AACGCAG193300.029.2395786
TATCAAC202950.028.2387242
ACGCAGA223600.025.3164547
CGCAGAG232300.024.3120378
GTACATG214950.020.4173971
GCAGAGT282850.020.0132889
TACATGG214100.019.765642
ACATGGG217050.019.0137733
GAGTACT165400.018.90359112-13
CAGAGTA271700.018.5649210-11
AGAGTAC250700.017.60827610-11
CATGGGG152000.016.68634
GTACTTT186200.016.67513814-15
GTGGTAT52500.016.3536721
AGTACTT186400.015.14910812-13
Produced by FastQC (version 0.11.5)