FastQCFastQC Report
Fri 27 May 2016
SRR2138996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668502
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123600.051.0797961
GTATCAA224750.036.853751
TCAACGC267400.030.9734384
ATCAACG270050.030.6533643
CAACGCA276400.029.931835
TATCAAC285250.029.312212
AACGCAG284000.029.222336
ACGCAGA327800.025.330997
CGCAGAG338700.024.464348
GTACATG302850.021.2065071
GTGGTAT65500.021.0871181
GCAGAGT396650.020.945059
TACATGG309450.020.8668542
ACATGGG306150.020.3645153
TGGTATC61850.020.2885972
GAGTACT223600.019.93159312-13
CAGAGTA386900.018.91121710-11
AGAGTAC352200.018.7149110-11
CATGGGG226200.017.97044
GTACTTT258100.017.25915714-15
Produced by FastQC (version 0.11.5)