##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10668502
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.421033805870778	18.0	18.0	30.0	18.0	33.0
2	31.342981142057244	32.0	32.0	33.0	27.0	33.0
3	31.871734850872222	33.0	31.0	33.0	29.0	33.0
4	32.585591585397836	33.0	33.0	33.0	31.0	34.0
5	33.0899689572163	33.0	33.0	34.0	33.0	34.0
6	37.02606495269907	38.0	38.0	38.0	36.0	38.0
7	37.146955495720015	38.0	38.0	38.0	36.0	38.0
8	37.15547346759648	38.0	38.0	38.0	36.0	38.0
9	37.34048669625783	38.0	38.0	38.0	37.0	38.0
10-11	37.37908100874893	38.0	38.0	38.0	37.0	38.0
12-13	37.41357708889214	38.0	38.0	38.0	37.0	38.0
14-15	37.41030751083892	38.0	38.0	38.0	37.0	38.0
16-17	37.34486927030618	38.0	38.0	38.0	37.0	38.0
18-19	37.398938951316694	38.0	38.0	38.0	37.0	38.0
20-21	37.43592546350029	38.0	38.0	38.0	37.0	38.0
22-23	37.45279328107109	38.0	38.0	38.0	37.0	38.0
24-25	37.456744276414895	38.0	38.0	38.0	37.0	38.0
26-27	37.4304861990766	38.0	38.0	38.0	37.0	38.0
28-29	37.408112277564456	38.0	38.0	38.0	37.0	38.0
30-31	37.31195482228486	38.0	38.0	38.0	37.0	38.0
32-33	37.3767862563698	38.0	38.0	38.0	37.0	38.0
34-35	37.38627897673362	38.0	38.0	38.0	37.0	38.0
36-37	37.36990878058946	38.0	38.0	38.0	37.0	38.0
38-39	37.35128481535372	38.0	38.0	38.0	37.0	38.0
40-41	37.33866540013622	38.0	38.0	38.0	37.0	38.0
42-43	37.32522669323053	38.0	38.0	38.0	37.0	38.0
44-45	37.29380879174366	38.0	38.0	38.0	37.0	38.0
46-47	37.268183030593114	38.0	38.0	38.0	37.0	38.0
48-49	37.23913510030351	38.0	38.0	38.0	37.0	38.0
50-51	37.19487643504466	38.0	38.0	38.0	37.0	38.0
52-53	37.170025301045996	38.0	38.0	38.0	37.0	38.0
54-55	37.15594216176846	38.0	38.0	38.0	36.5	38.0
56-57	37.14570627452646	38.0	38.0	38.0	36.0	38.0
58-59	37.13471258459667	38.0	38.0	38.0	36.0	38.0
60-61	37.1323299982989	38.0	38.0	38.0	36.0	38.0
62-63	37.137470067759594	38.0	38.0	38.0	36.0	38.0
64-65	37.139115643296165	38.0	38.0	38.0	36.0	38.0
66-67	37.119591390398185	38.0	38.0	38.0	36.0	38.0
68-69	37.11836742549053	38.0	38.0	38.0	36.0	38.0
70-71	37.099078083113135	38.0	38.0	38.0	36.0	38.0
72-73	37.0861695140578	38.0	38.0	38.0	36.0	38.0
74-75	37.071155538573514	38.0	38.0	38.0	36.0	38.0
76-77	37.07069650101659	38.0	38.0	38.0	36.0	38.0
78-79	37.05034612847763	38.0	38.0	38.0	36.0	38.0
80-81	37.04467253219546	38.0	38.0	38.0	36.0	38.0
82-83	36.99077628884372	38.0	38.0	38.0	36.0	38.0
84-85	36.975830891437454	38.0	38.0	38.0	36.0	38.0
86-87	36.96467366757089	38.0	38.0	38.0	36.0	38.0
88-89	36.96457096915324	38.0	38.0	38.0	36.0	38.0
90-91	36.955051975167585	38.0	38.0	38.0	36.0	38.0
92-93	36.93320076514322	38.0	38.0	38.0	35.0	38.0
94-95	36.90464995291491	38.0	38.0	38.0	35.0	38.0
96-97	36.87015259408284	38.0	38.0	38.0	35.0	38.0
98-99	36.86898469465845	38.0	38.0	38.0	35.0	38.0
100	35.297635808136945	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	20.0
19	52.0
20	136.0
21	338.0
22	853.0
23	2095.0
24	4803.0
25	8861.0
26	15510.0
27	25409.0
28	39048.0
29	57172.0
30	78904.0
31	105993.0
32	140736.0
33	191497.0
34	283134.0
35	505302.0
36	1527099.0
37	7681531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99215616213036	18.686166061552033	16.578513084592384	23.74316469172523
2	14.439103113926324	20.84322529372162	42.04142690204517	22.67624469030688
3	18.17968445804294	25.797586202823975	30.599628701386568	25.423100637746515
4	12.440815391452444	17.82533408235747	38.889135209886895	30.84471531630319
5	13.362954611410776	37.39517475810425	35.59428591354219	13.64758471694279
6	29.973477063602743	37.77826540220923	18.843657713144733	13.404599821043291
7	26.482443364588583	32.45176314350412	23.24802488671793	17.817768605189368
8	24.092482712193334	37.13853172638483	21.189441591706128	17.57954396971571
9	25.44345808115362	17.03948911949337	21.431969287479767	36.08508351187324
10-11	24.1799873140114	26.81343132558083	28.981297493369006	20.025283867038766
12-13	24.889133909253044	24.36252290738877	29.12154669151739	21.626796491840793
14-15	22.104855958221687	25.696231767121567	26.661971849468653	25.536940425188092
16-17	21.765759616485987	28.770285650225308	27.391877510075922	22.07207722321278
18-19	21.80607924149051	27.962904257786143	29.078998157379548	21.152018343343798
20-21	22.520894913251173	27.091975299731374	29.00149094717795	21.385638839839505
22-23	22.29654631381929	27.051808151730132	28.82738112032882	21.824264414121757
24-25	22.074888684278072	27.160712735598512	28.90458092623455	21.859817653888864
26-27	21.969458654875638	27.314191707908126	28.884664860775118	21.83168477644112
28-29	21.970497913756866	27.23916967377844	28.969337141963887	21.820995270500806
30-31	22.155691544658787	27.17843578395077	28.876074569181164	21.789798102209282
32-33	21.991683970974808	27.36738863776746	28.818493132984575	21.822434258273155
34-35	22.179397973787285	27.27955050467008	28.809557660439893	21.73149386110274
36-37	22.0476973870582	27.2556537944653	28.81591938701741	21.8807294314591
38-39	21.93509582047519	27.332161321533942	28.93725390703657	21.795488950954297
40-41	22.142656596602485	27.192521512795754	28.82292888364456	21.841893006957203
42-43	22.038093119939333	27.283088952690893	28.69613500028375	21.982682927086024
44-45	22.063445443533677	27.372744434723757	28.625704467935986	21.938105653806584
46-47	22.15336246578564	27.297604718845104	28.402402313393356	22.146630501975903
48-49	22.08217830374215	27.37720446924542	28.44333537769102	22.097281849321412
50-51	22.03438930560522	27.541904892587198	28.352498109163648	22.07120769264393
52-53	22.21916704920744	27.456242335205182	28.268434287915646	22.05615632767173
54-55	22.193443555016735	27.393457111540464	28.266726490037865	22.146372843404933
56-57	22.152066941787915	27.503710292382273	28.2431325577314	22.101090208098412
58-59	22.1021345107005	27.49779424580093	28.3163908895969	22.083680353901677
60-61	22.173885567303927	27.522759759218694	28.275086230585373	22.028268442892003
62-63	22.16439816346588	27.603707417687644	28.27248768228025	21.95940673656623
64-65	22.12416560864228	27.53141084955824	28.251417877469315	22.093005664330168
66-67	22.127870907147162	27.581608275832565	28.113942586326328	22.176578230693945
68-69	22.07987389735442	27.596020929472658	28.179411282803596	22.144693890369318
70-71	22.11590401304272	27.37163473891007	28.23048812691994	22.281973121127265
72-73	22.206446391899267	27.360703528131005	28.140288554010823	22.292561525958902
74-75	22.06445279816404	27.53739430258489	28.10438665617092	22.29376624308015
76-77	22.24411560689414	27.43842613278906	28.00750475661731	22.30995350369949
78-79	22.213516260471195	27.448706280829043	27.944449047458132	22.39332841124163
80-81	22.1933406929828	27.54754856901082	27.90679767914564	22.352313058860734
82-83	22.244922787500983	27.50807023294839	27.969022350025803	22.277984629524827
84-85	22.300941174730664	27.51341090175669	27.907646906384088	22.278001017128556
86-87	22.24967135318068	27.529652769240798	27.969670373308368	22.251005504270154
88-89	22.294822551763147	27.531279878087084	27.911531148470342	22.262366421679427
90-91	22.320061196763717	27.572132969334234	27.87908104662807	22.228724787273975
92-93	22.37333995495165	27.56264748485806	27.894238996271998	22.16977356391829
94-95	22.26054493207404	27.615798196543935	27.865629319843894	22.258027551538135
96-97	22.38318634067079	27.701398148676116	27.929533622784135	21.985881887868956
98-99	23.44329663890221	29.576773991512624	28.174443094970364	18.8054862746148
100	22.257540123605942	30.49258471732255	22.163627528364398	25.08624763070711
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2279.0
1	2172.0
2	2839.5
3	3911.0
4	4529.5
5	5487.5
6	6806.5
7	10187.0
8	14081.5
9	15605.5
10	15164.0
11	14466.5
12	15382.0
13	17794.0
14	22345.5
15	27466.0
16	30616.0
17	31796.5
18	31266.0
19	29565.0
20	27987.0
21	27836.0
22	30368.5
23	36440.5
24	46496.0
25	60852.0
26	78558.0
27	98835.0
28	120168.0
29	144133.0
30	168393.0
31	191282.5
32	216613.5
33	242412.5
34	267155.5
35	290051.5
36	315634.0
37	339535.5
38	358875.5
39	377575.5
40	393936.5
41	407506.0
42	416855.5
43	427843.0
44	436978.5
45	443596.5
46	445408.0
47	440284.0
48	436934.5
49	432465.0
50	424324.5
51	411541.0
52	394806.0
53	373661.5
54	349166.5
55	324623.5
56	298281.0
57	269168.0
58	239478.5
59	207096.5
60	171724.0
61	138270.5
62	108621.5
63	83294.5
64	61780.0
65	44916.0
66	33077.0
67	24999.0
68	18921.5
69	13943.5
70	10135.0
71	7246.5
72	5034.5
73	3394.5
74	2208.5
75	1388.0
76	870.5
77	507.0
78	295.0
79	175.0
80	114.5
81	80.5
82	59.0
83	40.0
84	30.0
85	24.5
86	17.5
87	17.0
88	14.5
89	6.5
90	2.5
91	2.0
92	2.5
93	3.0
94	2.0
95	0.5
96	0.5
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00701129361929163
3	0.0
4	0.0037399814894349737
5	0.0025308145417229145
6	0.0
7	0.0
8	0.0
9	1.7809435663976067E-4
10-11	3.6556210047108766E-4
12-13	9.37338719156635E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.077194901776268E-4
24-25	0.0013920716317877729
26-27	0.0
28-29	0.002006706619820382
30-31	0.0029698870345053956
32-33	5.920251394548106E-4
34-35	1.883096963863934E-5
36-37	0.001067027353387845
38-39	0.0022093857646809773
40-41	0.0015812116243629848
42-43	0.0
44-45	0.0
46-47	0.0014432347210115892
48-49	5.362753725285627E-4
50-51	0.0017955080215792593
52-53	0.0026198258254930564
54-55	0.0019897722727962902
56-57	0.0019821118648017974
58-59	0.0022112335084697276
60-61	9.663864483274178E-4
62-63	8.047403484892426E-4
64-65	0.0034256303095667236
66-67	0.0025058300590487226
68-69	7.291332480594639E-5
70-71	0.002903735853082938
72-73	0.010229825309848013
74-75	0.012138147808280009
76-77	0.004898749146617465
78-79	0.004597982353290746
80-81	3.663952352432405E-4
82-83	1.2132771343364709E-4
84-85	8.339846753647933E-5
86-87	0.001855444608812297
88-89	0.0045967980502791324
90-91	4.906854493449149E-4
92-93	0.0013705272153305562
94-95	0.002389419580334205
96-97	0.0027182815460406137
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	69.0
20-21	247.0
22-23	512.0
24-25	654.0
26-27	1836.0
28-29	4750.0
30-31	14802.0
32-33	18934.0
34-35	26730.0
36-37	42287.0
38-39	47685.0
40-41	47764.0
42-43	55034.0
44-45	65605.0
46-47	69105.0
48-49	65095.0
50-51	59351.0
52-53	56462.0
54-55	61754.0
56-57	63910.0
58-59	66962.0
60-61	66133.0
62-63	65631.0
64-65	70664.0
66-67	76634.0
68-69	77598.0
70-71	80762.0
72-73	72672.0
74-75	71811.0
76-77	76188.0
78-79	78055.0
80-81	77804.0
82-83	73853.0
84-85	72791.0
86-87	75743.0
88-89	79027.0
90-91	80924.0
92-93	80350.0
94-95	96535.0
96-97	329994.0
98-99	746305.0
100-101	7449480.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.42265837320028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.94753917422554	76.83557910389916
2	7.1102123090041145	12.14746474065964
3	1.6789557233196555	4.302625835305929
4	0.5843631346859821	1.9967140968069226
5	0.25210709187626945	1.0767828991403792
6	0.13838851507996683	0.7092908907870289
7	0.08160236707779561	0.48794837877317143
8	0.05338777032462081	0.36484202125975707
9	0.03600203614606971	0.27678506710007894
>10	0.11390258627853138	1.506041281312784
>50	0.0026116171705556457	0.14642852115953747
>100	8.736228131974578E-4	0.10866661616160038
>500	3.6036280513680466E-5	0.020947307738593053
>1k	1.801571720668472E-5	0.01988323989532901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8120161574699053E-5	0.0	0.0	0.0
3	0.0	2.8120161574699053E-5	0.0	1.87467743831327E-5	0.0
4	0.0	5.6240323149398106E-5	0.0	1.87467743831327E-5	0.0
5	0.0	6.561371034096446E-5	0.0	1.87467743831327E-5	0.0
6	9.373387191566351E-5	6.561371034096446E-5	0.0	2.8120161574699053E-5	0.0
7	9.373387191566351E-5	7.49870975325308E-5	0.0	2.8120161574699053E-5	0.0
8	9.373387191566351E-5	7.49870975325308E-5	0.0	2.8120161574699053E-5	0.0
9	9.373387191566351E-5	1.4060080787349526E-4	0.0	7.49870975325308E-5	0.0
10-11	9.84205655114467E-5	1.4060080787349526E-4	0.0	1.2185403349036257E-4	0.0
12-13	1.0310725910722987E-4	1.4060080787349526E-4	0.0	1.4528750146927845E-4	0.0
14-15	1.0779395270301304E-4	1.7809435663976067E-4	0.0	1.499741950650616E-4	4.686693595783175E-6
16-17	1.6403427585241114E-4	1.921544374271102E-4	0.0	1.734076630439775E-4	9.37338719156635E-6
18-19	1.6872096944819433E-4	2.062145182144597E-4	0.0	1.8746774383132702E-4	9.37338719156635E-6
20-21	1.6872096944819433E-4	2.2496129259759243E-4	0.0	2.062145182144597E-4	9.37338719156635E-6
22-23	1.734076630439775E-4	2.3433467978915877E-4	0.0	2.2496129259759243E-4	9.37338719156635E-6
24-25	1.7809435663976067E-4	2.4839476057650833E-4	0.0	2.2496129259759243E-4	9.37338719156635E-6
26-27	1.7809435663976067E-4	2.6245484136385783E-4	0.0	2.952616965343401E-4	9.37338719156635E-6
28-29	1.7809435663976067E-4	2.67141534959641E-4	0.0	5.436564571108484E-4	9.37338719156635E-6
30-31	1.7809435663976067E-4	3.280685517048223E-4	0.0	0.0012982141260319397	9.37338719156635E-6
32-33	1.7809435663976067E-4	3.3744193889638865E-4	0.0	0.0030088572884927984	9.37338719156635E-6
34-35	1.921544374271102E-4	3.843088748542204E-4	0.0	0.005305337150426555	9.37338719156635E-6
36-37	2.0621451821445973E-4	4.4054919800361853E-4	0.0	0.008890657751200685	9.37338719156635E-6
38-39	2.0621451821445973E-4	4.592959723867512E-4	0.0	0.01745793364429233	9.37338719156635E-6
40-41	2.0621451821445973E-4	5.014762147487997E-4	0.0	0.027210943017117115	9.37338719156635E-6
42-43	2.0621451821445973E-4	5.202229891319325E-4	0.0	0.03407226244134369	9.37338719156635E-6
44-45	2.0621451821445973E-4	5.717766186855474E-4	0.0	0.041631899211341944	9.37338719156635E-6
46-47	2.0621451821445973E-4	6.092701674518128E-4	0.0	0.050372582767477574	9.37338719156635E-6
48-49	2.0621451821445973E-4	6.092701674518128E-4	0.0	0.05910857963001741	9.37338719156635E-6
50-51	2.2027459900180927E-4	6.327036354307287E-4	0.0	0.06812577810830424	9.37338719156635E-6
52-53	2.2496129259759243E-4	6.373903290265119E-4	0.0	0.08909873194943395	9.37338719156635E-6
54-55	2.2496129259759243E-4	6.467637162180782E-4	0.0	0.11523173543952094	9.37338719156635E-6
56-57	2.2496129259759243E-4	6.561371034096446E-4	0.0	0.15291743864321344	1.4060080787349525E-5
58-59	2.2496129259759243E-4	6.561371034096446E-4	0.0	0.18167030385334323	1.87467743831327E-5
60-61	2.483947605765083E-4	6.701971841969941E-4	0.0	0.19483991285749397	1.87467743831327E-5
62-63	2.999483901301232E-4	6.842572649843437E-4	0.0	0.20531467304406936	1.87467743831327E-5
64-65	2.999483901301232E-4	7.967379112831398E-4	0.0	0.21030131502998267	1.87467743831327E-5
66-67	3.093217773216896E-4	8.01424604878923E-4	0.0	0.21518484975678873	2.3433467978915877E-5
68-69	3.093217773216896E-4	8.107979920704894E-4	0.0	0.22021835867865985	3.280685517048223E-5
70-71	3.186951645132559E-4	8.154846856662726E-4	0.0	0.22428640871979966	3.74935487662654E-5
72-73	3.5618871327952134E-4	8.154846856662726E-4	0.0	0.2273374462506545	3.74935487662654E-5
74-75	3.6087540687530453E-4	8.342314600494052E-4	0.0	0.22992918780912258	3.74935487662654E-5
76-77	3.655621004710877E-4	8.436048472409716E-4	0.0	0.23263809670748525	3.74935487662654E-5
78-79	3.655621004710877E-4	8.52978234432538E-4	0.0	0.23407691164139072	3.74935487662654E-5
80-81	3.655621004710877E-4	8.576649280283211E-4	0.0	0.23468618180884251	3.74935487662654E-5
82-83	3.7024879406687085E-4	8.623516216241043E-4	0.0	0.23506580399010096	3.74935487662654E-5
84-85	3.7493548766265403E-4	8.623516216241043E-4	0.0	0.23525795842752806	3.74935487662654E-5
86-87	3.889955684500036E-4	8.623516216241043E-4	0.0	0.2353844991546142	3.74935487662654E-5
88	4.030556492373531E-4	8.81098396007237E-4	0.0	0.2354126193161889	3.74935487662654E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12360	0.0	51.079796	1
GTATCAA	22475	0.0	36.85375	1
TCAACGC	26740	0.0	30.973438	4
ATCAACG	27005	0.0	30.653364	3
CAACGCA	27640	0.0	29.93183	5
TATCAAC	28525	0.0	29.31221	2
AACGCAG	28400	0.0	29.22233	6
ACGCAGA	32780	0.0	25.33099	7
CGCAGAG	33870	0.0	24.46434	8
GTACATG	30285	0.0	21.206507	1
GTGGTAT	6550	0.0	21.087118	1
GCAGAGT	39665	0.0	20.94505	9
TACATGG	30945	0.0	20.866854	2
ACATGGG	30615	0.0	20.364515	3
TGGTATC	6185	0.0	20.288597	2
GAGTACT	22360	0.0	19.931593	12-13
CAGAGTA	38690	0.0	18.911217	10-11
AGAGTAC	35220	0.0	18.71491	10-11
CATGGGG	22620	0.0	17.9704	4
GTACTTT	25810	0.0	17.259157	14-15
>>END_MODULE