FastQCFastQC Report
Fri 27 May 2016
SRR2138995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7019641
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64400.045.5129471
GTATCAA118550.037.2146261
TCAACGC138350.031.979894
ATCAACG140550.031.6030253
CAACGCA140750.031.371595
AACGCAG148250.029.9000726
TATCAAC151600.029.6473772
ACGCAGA169150.026.2056487
CGCAGAG177850.024.923738
CTTATAC59200.022.8058221
GTACATG175650.020.3563921
GCAGAGT218750.020.3035169
TACATGG177100.020.2143152
TATACCG5000.020.0311015
TATACAC84350.019.7213953
ACATGGG176900.019.6688213
AGAGTAC191550.019.0152210-11
TATGCCG40550.017.97223342-43
CTCGTAT40300.017.9120238-39
GAGTACT123800.017.6035212-13
Produced by FastQC (version 0.11.5)