##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7019641
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.963831483689834	18.0	18.0	30.0	18.0	33.0
2	31.227659505664178	32.0	32.0	33.0	27.0	33.0
3	31.831435111852585	33.0	31.0	33.0	29.0	33.0
4	32.57223695627739	33.0	33.0	33.0	31.0	34.0
5	33.088759952253966	33.0	33.0	34.0	33.0	34.0
6	37.040507912014306	38.0	38.0	38.0	36.0	38.0
7	37.16424543648315	38.0	38.0	38.0	36.0	38.0
8	37.17358907670634	38.0	38.0	38.0	36.0	38.0
9	37.35755261558248	38.0	38.0	38.0	37.0	38.0
10-11	37.39737822774698	38.0	38.0	38.0	37.0	38.0
12-13	37.43451374507613	38.0	38.0	38.0	37.0	38.0
14-15	37.43097788049275	38.0	38.0	38.0	37.0	38.0
16-17	37.36532203000125	38.0	38.0	38.0	37.0	38.0
18-19	37.41856414024592	38.0	38.0	38.0	37.0	38.0
20-21	37.45550780948282	38.0	38.0	38.0	37.0	38.0
22-23	37.47164568958463	38.0	38.0	38.0	37.0	38.0
24-25	37.477851744063265	38.0	38.0	38.0	37.0	38.0
26-27	37.452453742070134	38.0	38.0	38.0	37.0	38.0
28-29	37.42822452411873	38.0	38.0	38.0	37.0	38.0
30-31	37.33216078471611	38.0	38.0	38.0	37.0	38.0
32-33	37.39766367324201	38.0	38.0	38.0	37.0	38.0
34-35	37.406892904823714	38.0	38.0	38.0	37.0	38.0
36-37	37.39081217047142	38.0	38.0	38.0	37.0	38.0
38-39	37.36894813509437	38.0	38.0	38.0	37.0	38.0
40-41	37.36607743212818	38.0	38.0	38.0	37.0	38.0
42-43	37.356298099307196	38.0	38.0	38.0	37.0	38.0
44-45	37.32955275943242	38.0	38.0	38.0	37.0	38.0
46-47	37.310571302008064	38.0	38.0	38.0	37.0	38.0
48-49	37.287542809671095	38.0	38.0	38.0	37.0	38.0
50-51	37.24527391473309	38.0	38.0	38.0	37.0	38.0
52-53	37.2214947608274	38.0	38.0	38.0	37.0	38.0
54-55	37.20550370603293	38.0	38.0	38.0	37.0	38.0
56-57	37.19498956460703	38.0	38.0	38.0	37.0	38.0
58-59	37.18200582816095	38.0	38.0	38.0	36.5	38.0
60-61	37.178021466077055	38.0	38.0	38.0	36.0	38.0
62-63	37.18227273024646	38.0	38.0	38.0	36.0	38.0
64-65	37.18246670186373	38.0	38.0	38.0	36.0	38.0
66-67	37.16159872751303	38.0	38.0	38.0	36.0	38.0
68-69	37.15657667915657	38.0	38.0	38.0	36.0	38.0
70-71	37.136869817997336	38.0	38.0	38.0	36.0	38.0
72-73	37.12300097005231	38.0	38.0	38.0	36.0	38.0
74-75	37.10955414651586	38.0	38.0	38.0	36.0	38.0
76-77	37.10959351793004	38.0	38.0	38.0	36.0	38.0
78-79	37.09114707091524	38.0	38.0	38.0	36.0	38.0
80-81	37.085756573127284	38.0	38.0	38.0	36.0	38.0
82-83	37.034718134965665	38.0	38.0	38.0	36.0	38.0
84-85	37.02160915333222	38.0	38.0	38.0	36.0	38.0
86-87	37.0105598950082	38.0	38.0	38.0	36.0	38.0
88-89	37.013265518087415	38.0	38.0	38.0	36.0	38.0
90-91	37.001375575474746	38.0	38.0	38.0	36.0	38.0
92-93	36.98157330667537	38.0	38.0	38.0	36.0	38.0
94-95	36.950462874518706	38.0	38.0	38.0	35.5	38.0
96-97	36.91838898554224	38.0	38.0	38.0	35.0	38.0
98-99	36.92076772485365	38.0	38.0	38.0	35.0	38.0
100	35.37124646213574	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	15.0
19	31.0
20	78.0
21	193.0
22	530.0
23	1325.0
24	2797.0
25	5500.0
26	9562.0
27	15724.0
28	23942.0
29	35111.0
30	48683.0
31	64973.0
32	86093.0
33	118948.0
34	178782.0
35	323438.0
36	1004607.0
37	5099306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51021982463206	18.62553939724268	16.72665311516643	24.137587662958833
2	14.139135816328915	20.771044268000313	42.232313970511555	22.857505945159218
3	17.87987448360963	25.88756319589563	30.723593984364726	25.50896833613001
4	12.263670737247718	17.87546811397171	39.0583599061514	30.802501242629166
5	13.28325357897391	37.43066071939904	35.829941576785714	13.456144124841336
6	29.945790675050194	37.98652951055474	18.86226090479556	13.205418909599508
7	26.420225763682215	32.37010268758758	23.39290570557668	17.81676584315352
8	24.015900528246387	37.20505079960642	21.318554609844007	17.460494062303187
9	25.302939885552323	17.014166137317552	21.36934056467673	36.313553412453395
10-11	24.072844979193313	26.740361549537088	29.187638246463234	19.999155224806362
12-13	24.80776615269685	24.14445583075088	29.253335694327937	21.794442322224334
14-15	21.968317183172186	25.513014127075728	26.62455245218381	25.894116237568277
16-17	21.890335987267726	28.678311326747334	27.261458242665114	22.16989444331982
18-19	21.921348969270653	27.9812457645626	28.756449225822234	21.340956040344512
20-21	22.595838827782472	27.1663586356144	28.58912090879453	21.648681627808596
22-23	22.309261536951432	27.18069621495207	28.487608466828824	22.02243378126768
24-25	22.1408829674266	27.290665533365782	28.57600083886301	21.992450660344613
26-27	22.141013802692605	27.478545537544473	28.47110071476169	21.909339945001236
28-29	22.107220926422936	27.410715138221253	28.51805705446922	21.964006880886586
30-31	22.361555171905092	27.262538913771976	28.443743596665332	21.932162317657593
32-33	22.11412280064778	27.40453500624084	28.488094914238644	21.993247278872733
34-35	22.228454955684764	27.29920804466109	28.522695530851138	21.949641468803012
36-37	22.16415942887996	27.374985727163033	28.47950248476464	21.981352359192368
38-39	22.04422457417539	27.353590338997552	28.640987993170558	21.961197093656494
40-41	22.246604077328122	27.216075154084233	28.563969019731505	21.97335174885614
42-43	22.182496244815837	27.209235510861674	28.511119902138088	22.097148342184404
44-45	22.13770108148669	27.374143473473218	28.410350199170466	22.077805245869627
46-47	22.30504634801933	27.22377434220536	28.179847242764026	22.291332067011286
48-49	22.208174161096792	27.330298192420038	28.210974975811986	22.25055267067118
50-51	22.11805033347678	27.43642796410155	28.218292902655158	22.227228799766515
52-53	22.26811625075146	27.38749384436411	28.165187354832007	22.179202550052423
54-55	22.26033645456568	27.282253853286893	28.142760707486026	22.314648984661396
56-57	22.309203626103614	27.466337181576762	28.03400958834944	22.190449603970183
58-59	22.164861443884444	27.55899154343612	28.20747194957809	22.068675063101345
60-61	22.2714974769352	27.65946488751766	28.013407017484344	22.0556306180628
62-63	22.18629361568195	27.842472022760283	27.98312034654461	21.988114015013153
64-65	22.1522115531558	27.741747934824456	27.993878160167547	22.11216235185219
66-67	22.164033101935924	27.775091000215152	27.841196705362535	22.219679192486392
68-69	22.13862909234897	27.70396435533841	27.95093008074953	22.206476471563086
70-71	22.181562948030013	27.46846766008443	27.981471622190185	22.368497769695374
72-73	22.32984873671899	27.368375115802973	27.94250665187738	22.359269495600653
74-75	22.232164142904647	27.49064858090014	27.910210797028647	22.36697647916657
76-77	22.315713315096847	27.47736200408052	27.788695520416535	22.418229160406103
78-79	22.272201358653202	27.465346346924402	27.760011112581246	22.502441181841153
80-81	22.31159453787032	27.50918785390822	27.74533174546111	22.43388586276035
82-83	22.384306859900466	27.486539412073213	27.78687098503876	22.34228274298756
84-85	22.433430923514646	27.468164563521334	27.755026271917004	22.343378241047017
86-87	22.30121310944731	27.512736545432432	27.796280390914973	22.38976995420529
88-89	22.376187708264574	27.49792611278697	27.753369524015696	22.372516654932763
90-91	22.405077445866578	27.5661948816249	27.70777290226181	22.320954770246722
92-93	22.433622399056386	27.52901864043152	27.751202455972983	22.286156504539107
94-95	22.330420193916613	27.608376476494968	27.707023090909573	22.354180238678843
96-97	22.442523211641973	27.65929670733247	27.774897642110336	22.123282438915222
98-99	23.50471040382868	29.592990950848563	28.030515298444296	18.87178334687846
100	22.388640914617465	30.476454521141783	21.972259344017193	25.162645220223556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	623.0
1	708.0
2	1262.5
3	1906.0
4	2307.0
5	2885.5
6	3565.0
7	5081.5
8	6748.5
9	7441.0
10	7434.5
11	7410.5
12	8057.0
13	9413.0
14	11864.5
15	14576.5
16	16382.0
17	17217.0
18	17217.0
19	16600.0
20	16160.5
21	16774.5
22	19101.5
23	23479.5
24	30162.5
25	39801.5
26	51512.0
27	64839.5
28	79788.0
29	95524.0
30	110215.5
31	124806.5
32	141501.0
33	158726.0
34	174771.5
35	189641.0
36	206520.0
37	221140.0
38	232411.0
39	244559.0
40	256799.5
41	266729.5
42	274178.5
43	287001.0
44	295939.5
45	297738.0
46	297288.0
47	294326.5
48	293024.5
49	289256.0
50	283302.0
51	274491.0
52	264413.0
53	251538.0
54	234315.5
55	216457.5
56	197240.0
57	177031.5
58	157173.5
59	135709.0
60	112235.5
61	89875.0
62	70244.0
63	53485.5
64	39398.0
65	28757.5
66	20950.0
67	15820.0
68	11966.5
69	8779.5
70	6414.5
71	4531.5
72	3231.5
73	2258.0
74	1509.0
75	957.5
76	565.0
77	339.5
78	204.5
79	114.0
80	63.0
81	39.5
82	31.5
83	23.5
84	15.0
85	10.5
86	9.5
87	8.5
88	7.0
89	5.0
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006909185241809375
3	0.0
4	0.003561435691654317
5	0.002436022013091553
6	0.0
7	0.0
8	0.0
9	1.9944039873264174E-4
10-11	3.1340634086557986E-4
12-13	7.1228713833086335E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.336787464379184E-4
24-25	0.0014602955609721835
26-27	0.0
28-29	0.0018597030075672813
30-31	0.0030088394998738356
32-33	5.426481070541898E-4
34-35	1.4306195648527386E-5
36-37	0.0011762400654333743
38-39	0.0024916985390436917
40-41	0.0015770429995242104
42-43	0.0
44-45	0.0
46-47	0.0013015528923152493
48-49	5.475803276291496E-4
50-51	0.001690063624567033
52-53	0.002463316795418021
54-55	0.0018146516328175154
56-57	0.0017347664777059026
58-59	0.002066104203469942
60-61	8.596529780489914E-4
62-63	6.645610385018075E-4
64-65	0.0032601648087228624
66-67	0.0023129797129725466
68-69	7.11602352177855E-5
70-71	0.00268785858386382
72-73	0.010329210370913652
74-75	0.01177258163303638
76-77	0.005094051064141543
78-79	0.0041794089178199606
80-81	3.1593280741051995E-4
82-83	9.223161605889944E-5
84-85	5.071959267433253E-5
86-87	0.0016279193452048144
88-89	0.004754088946339145
90-91	4.5110440334285713E-4
92-93	0.001120668703015089
94-95	0.0022362934876216734
96-97	0.002546329752572062
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	129.0
22-23	276.0
24-25	363.0
26-27	1009.0
28-29	2968.0
30-31	9442.0
32-33	11853.0
34-35	16124.0
36-37	26675.0
38-39	31394.0
40-41	31392.0
42-43	35954.0
44-45	41634.0
46-47	42917.0
48-49	41758.0
50-51	38640.0
52-53	36926.0
54-55	40058.0
56-57	41115.0
58-59	43307.0
60-61	44446.0
62-63	43815.0
64-65	47128.0
66-67	52332.0
68-69	54801.0
70-71	60083.0
72-73	48801.0
74-75	47278.0
76-77	48749.0
78-79	51511.0
80-81	51123.0
82-83	48785.0
84-85	48220.0
86-87	49709.0
88-89	51827.0
90-91	53037.0
92-93	53036.0
94-95	63495.0
96-97	220554.0
98-99	492396.0
100-101	4894549.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.21325595964899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0667132658203	75.00597921739188
2	7.536999964304224	12.69430614323634
3	1.8686945815429827	4.721065653176651
4	0.703667596017807	2.370325574958339
5	0.32780482057002364	1.3802755629735106
6	0.16573103807632314	0.837405019798784
7	0.10457860190332822	0.6164833198991051
8	0.056496551118516196	0.3806206816144798
9	0.04204450563057396	0.31866342429279854
>10	0.12577425527135588	1.5430852193708873
>50	0.0011483032193536837	0.06086293595822731
>100	2.913706235995213E-4	0.03711881266582221
>500	5.51459016222924E-5	0.03380843466308965
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4245742766617267E-5	1.4245742766617267E-5
2	0.0	2.8491485533234534E-5	0.0	1.4245742766617267E-5	1.4245742766617267E-5
3	0.0	2.8491485533234534E-5	0.0	1.4245742766617267E-5	1.4245742766617267E-5
4	0.0	4.27372282998518E-5	0.0	1.4245742766617267E-5	1.4245742766617267E-5
5	0.0	4.27372282998518E-5	0.0	1.4245742766617267E-5	1.4245742766617267E-5
6	0.0	4.27372282998518E-5	0.0	1.4245742766617267E-5	1.4245742766617267E-5
7	0.0	4.27372282998518E-5	0.0	2.8491485533234534E-5	1.4245742766617267E-5
8	0.0	4.27372282998518E-5	0.0	2.8491485533234534E-5	1.4245742766617267E-5
9	0.0	1.5670317043278993E-4	0.0	1.1396594213293814E-4	1.4245742766617267E-5
10-11	0.0	1.5670317043278993E-4	0.0	1.282116848995554E-4	1.4245742766617267E-5
12-13	0.0	1.5670317043278993E-4	0.0	1.282116848995554E-4	1.4245742766617267E-5
14-15	0.0	2.4930049841580216E-4	0.0	1.5670317043278993E-4	3.561435691654317E-5
16-17	0.0	2.6354624118241944E-4	0.0	1.923175273493331E-4	4.27372282998518E-5
18-19	0.0	2.777919839490367E-4	0.0	1.9944039873264174E-4	4.27372282998518E-5
20-21	0.0	2.8491485533234536E-4	0.0	2.1368614149925902E-4	4.27372282998518E-5
22-23	0.0	3.1340634086557986E-4	0.0	2.350547556491849E-4	4.9860099683160435E-5
24-25	0.0	3.2765208363219714E-4	0.0	2.4217762703249355E-4	7.122871383308634E-5
26-27	0.0	3.418978263988144E-4	0.0	2.706691125657281E-4	8.54744565997036E-5
28-29	0.0	3.4902069778212306E-4	0.0	5.05723868214913E-4	8.54744565997036E-5
30-31	0.0	3.703893119320489E-4	0.0	0.001168150906862616	8.54744565997036E-5
32-33	0.0	3.846350546986662E-4	0.0	0.0025571108266077996	8.54744565997036E-5
34-35	0.0	4.701095112983698E-4	0.0	0.004259477087218563	8.54744565997036E-5
36-37	0.0	5.627068392813821E-4	0.0	0.0068165879138263625	8.54744565997036E-5
38-39	0.0	6.481812958810856E-4	0.0	0.014381077322900133	8.54744565997036E-5
40-41	0.0	6.837956527976288E-4	0.0	0.022764696941054395	8.54744565997036E-5
42-43	0.0	7.122871383308633E-4	0.0	0.0280498675074694	8.54744565997036E-5
44-45	0.0	7.906387235472583E-4	0.0	0.03380514758518278	8.54744565997036E-5
46-47	0.0	8.404988232304188E-4	0.0	0.040856790254658326	8.54744565997036E-5
48-49	0.0	8.618674373803447E-4	0.0	0.04730298885655264	9.972019936632087E-5
50-51	0.0	9.615876367466656E-4	0.0	0.05467516073827707	9.972019936632087E-5
52-53	0.0	9.687105081299742E-4	0.0	0.0740992310005597	1.0684307074962951E-4
54-55	0.0	9.972019936632086E-4	0.0	0.09871587450127435	1.1396594213293814E-4
56-57	0.0	0.001011447736429826	0.0	0.1373859432412569	1.1396594213293814E-4
58-59	0.0	0.0010256934791964432	0.0	0.1662264494722736	1.1396594213293814E-4
60-61	0.0	0.0010969221930295296	0.0	0.1772811458591686	1.1396594213293814E-4
62-63	0.0	0.0011254136785627642	0.0	0.18580722290498902	1.1396594213293814E-4
64-65	0.0	0.0013248540772954058	0.0	0.1896891878088922	1.1396594213293814E-4
66-67	0.0	0.0013390998200620231	0.0	0.19369224152631168	1.1396594213293814E-4
68-69	0.0	0.0013462226914453318	0.0	0.1976668037581979	1.1396594213293814E-4
70-71	0.0	0.0013533455628286404	0.0	0.20141343410581825	1.1396594213293814E-4
72-73	0.0	0.0013533455628286404	0.0	0.2042768284019083	1.1396594213293814E-4
74-75	0.0	0.001381837048361875	0.0	0.20670572754361655	1.1396594213293814E-4
76-77	0.0	0.0014103285338951095	0.0	0.20893518628659213	1.1396594213293814E-4
78-79	0.0	0.001438820019428344	0.0	0.21051646373368665	1.1396594213293814E-4
80-81	0.0	0.001438820019428344	0.0	0.21117889077233437	1.282116848995554E-4
82-83	0.0	0.001438820019428344	0.0	0.2116062630553329	1.4245742766617268E-4
84-85	0.0	0.001438820019428344	0.0	0.21179858058268222	1.4245742766617268E-4
86-87	0.0	0.0014886801191115043	0.0	0.21192679226758177	1.4245742766617268E-4
88	0.0	0.0015812774470945166	0.0	0.2119624066244983	1.5670317043278993E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6440	0.0	45.512947	1
GTATCAA	11855	0.0	37.214626	1
TCAACGC	13835	0.0	31.97989	4
ATCAACG	14055	0.0	31.603025	3
CAACGCA	14075	0.0	31.37159	5
AACGCAG	14825	0.0	29.900072	6
TATCAAC	15160	0.0	29.647377	2
ACGCAGA	16915	0.0	26.205648	7
CGCAGAG	17785	0.0	24.92373	8
CTTATAC	5920	0.0	22.805822	1
GTACATG	17565	0.0	20.356392	1
GCAGAGT	21875	0.0	20.303516	9
TACATGG	17710	0.0	20.214315	2
TATACCG	500	0.0	20.031101	5
TATACAC	8435	0.0	19.721395	3
ACATGGG	17690	0.0	19.668821	3
AGAGTAC	19155	0.0	19.01522	10-11
TATGCCG	4055	0.0	17.972233	42-43
CTCGTAT	4030	0.0	17.91202	38-39
GAGTACT	12380	0.0	17.60352	12-13
>>END_MODULE