FastQCFastQC Report
Fri 27 May 2016
SRR2138994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083133
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127350.044.026951
GTATCAA225350.032.82771
ATCAACG254250.030.2373183
TCAACGC254950.030.2054564
CAACGCA260150.029.6342285
AACGCAG270600.028.537766
TATCAAC282150.027.5254572
ACGCAGA304450.025.3078467
CGCAGAG317350.024.2654428
TACATGG281450.020.5991442
GTACATG277600.020.3381081
GCAGAGT378450.020.3134639
ACATGGG281850.020.045733
GAGTACT229750.018.91937312-13
AGAGTAC341100.018.70507210-11
CATGGGG217200.018.306574
CAGAGTA374400.018.1126410-11
GTGGTAT68450.016.9777581
GTACTTT263350.016.3329314-15
TATGCCG45100.015.89920342-43
Produced by FastQC (version 0.11.5)