##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11083133
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.665505593048465	18.0	18.0	25.0	18.0	33.0
2	30.49826244979646	32.0	27.0	32.0	27.0	33.0
3	31.50922144487484	33.0	31.0	33.0	29.0	33.0
4	32.44691243892859	33.0	33.0	33.0	31.0	34.0
5	32.86394469866959	33.0	33.0	34.0	32.0	34.0
6	37.13777268575591	38.0	38.0	38.0	36.0	38.0
7	37.260435023201474	38.0	38.0	38.0	36.0	38.0
8	37.31826406847234	38.0	38.0	38.0	37.0	38.0
9	37.405353793011415	38.0	38.0	38.0	37.0	38.0
10-11	37.43027183739471	38.0	38.0	38.0	37.0	38.0
12-13	37.45548167652595	38.0	38.0	38.0	37.0	38.0
14-15	37.44268709939689	38.0	38.0	38.0	37.0	38.0
16-17	37.3850556967962	38.0	38.0	38.0	37.0	38.0
18-19	37.41324943948611	38.0	38.0	38.0	37.0	38.0
20-21	37.43737572137168	38.0	38.0	38.0	37.0	38.0
22-23	37.44218288019556	38.0	38.0	38.0	37.0	38.0
24-25	37.44395734533455	38.0	38.0	38.0	37.0	38.0
26-27	37.41353257089233	38.0	38.0	38.0	37.0	38.0
28-29	37.38675363685478	38.0	38.0	38.0	37.0	38.0
30-31	37.29218232518387	38.0	38.0	38.0	37.0	38.0
32-33	37.35635180525447	38.0	38.0	38.0	37.0	38.0
34-35	37.365847897815115	38.0	38.0	38.0	37.0	38.0
36-37	37.34404951554454	38.0	38.0	38.0	37.0	38.0
38-39	37.32289158765491	38.0	38.0	38.0	37.0	38.0
40-41	37.31341621054587	38.0	38.0	38.0	37.0	38.0
42-43	37.29216426008885	38.0	38.0	38.0	37.0	38.0
44-45	37.25820572521437	38.0	38.0	38.0	37.0	38.0
46-47	37.23112395055381	38.0	38.0	38.0	37.0	38.0
48-49	37.19986438284326	38.0	38.0	38.0	37.0	38.0
50-51	37.15253045806376	38.0	38.0	38.0	36.5	38.0
52-53	37.12647015604554	38.0	38.0	38.0	36.0	38.0
54-55	37.105819142050464	38.0	38.0	38.0	36.0	38.0
56-57	37.093067555273706	38.0	38.0	38.0	36.0	38.0
58-59	37.077715962477285	38.0	38.0	38.0	36.0	38.0
60-61	37.07209746117415	38.0	38.0	38.0	36.0	38.0
62-63	37.07588679443516	38.0	38.0	38.0	36.0	38.0
64-65	37.07698494944507	38.0	38.0	38.0	36.0	38.0
66-67	37.053466387516124	38.0	38.0	38.0	36.0	38.0
68-69	37.048624997351055	38.0	38.0	38.0	36.0	38.0
70-71	37.026745179224676	38.0	38.0	38.0	36.0	38.0
72-73	37.0134114817036	38.0	38.0	38.0	36.0	38.0
74-75	36.99860812642873	38.0	38.0	38.0	36.0	38.0
76-77	36.99644347521257	38.0	38.0	38.0	36.0	38.0
78-79	36.974130339752335	38.0	38.0	38.0	36.0	38.0
80-81	36.96581431952333	38.0	38.0	38.0	36.0	38.0
82-83	36.91378660536766	38.0	38.0	38.0	35.5	38.0
84-85	36.89574726943883	38.0	38.0	38.0	35.0	38.0
86-87	36.8801772571765	38.0	38.0	38.0	35.0	38.0
88-89	36.88094847574139	38.0	38.0	38.0	35.0	38.0
90-91	36.86617364756745	38.0	38.0	38.0	35.0	38.0
92-93	36.84472213741176	38.0	38.0	38.0	35.0	38.0
94-95	36.81514530481049	38.0	38.0	38.0	35.0	38.0
96-97	36.779912557403	38.0	38.0	38.0	35.0	38.0
98-99	36.777854374411774	38.0	38.0	38.0	35.0	38.0
100	35.09048637415522	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	15.0
19	41.0
20	157.0
21	448.0
22	1121.0
23	2653.0
24	5551.0
25	10444.0
26	18096.0
27	29031.0
28	44171.0
29	63518.0
30	87394.0
31	115736.0
32	151327.0
33	204883.0
34	305693.0
35	552027.0
36	1769979.0
37	7720845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.48390766401522	17.651687478621795	19.67812711441792	23.18627774294507
2	14.205468130992383	21.006618809264005	42.25817317327208	22.529739886471535
3	18.319007811238933	25.751409822475285	30.474731287624174	25.4548510786616
4	12.426003682277472	18.13639404790209	38.882007865330245	30.555594404490193
5	13.311538907129108	37.385468281205846	35.96047841388164	13.342514397783406
6	29.933819254898413	37.95909514033622	18.771009966225254	13.336075638540112
7	26.270883873720546	32.465278545335515	23.492220115016213	17.77161746592773
8	23.79458949017394	37.5076343485186	21.255424797302354	17.442351364005106
9	25.098733434838998	17.190900580837987	21.64322788022331	36.0671381040997
10-11	24.04086527584359	26.927719645985377	29.121518917786688	19.909896160384346
12-13	24.727199167491605	24.3935930923494	29.337552297380938	21.54165544277806
14-15	21.901997386479074	25.802586687356367	26.814033540876935	25.481382385287628
16-17	21.60084607845092	28.941780270975727	27.55667102433942	21.900702626233937
18-19	21.611637250947002	28.08496478387474	29.25440847818031	21.048989486997947
20-21	22.253379531988905	27.27086282950389	29.14676626779927	21.32899137070794
22-23	22.084158537788085	27.222475722544342	29.000856928338255	21.69250881132932
24-25	21.914328531746847	27.359563577725986	29.02907459013786	21.697033300389304
26-27	21.89436263734826	27.408823254303115	29.035201880737738	21.661612227610885
28-29	21.843365201986778	27.4125406824391	29.04075896232255	21.703335153251572
30-31	21.961540865743334	27.344551408862667	29.04233453185031	21.65157319354369
32-33	21.828312129760764	27.47335055529892	28.990278582211364	21.708058732728954
34-35	21.992059224737304	27.395905830122057	29.01633002772907	21.595704917411567
36-37	21.907060278473047	27.431432785618938	28.929989420872577	21.73151751503545
38-39	21.78815589899413	27.488645963969937	29.048147507799083	21.67505062923685
40-41	21.950967816805544	27.329131529604005	28.951241136949367	21.768659516641083
42-43	21.92557084489446	27.45732822009266	28.798234339270145	21.818866595742737
44-45	21.931413367053878	27.496802379511426	28.767500581459387	21.804283671975313
46-47	21.99024745358408	27.514738349171225	28.514992928835987	21.980021268408713
48-49	21.91596180523592	27.632897700072494	28.45804255504997	21.993097939641608
50-51	21.88280171856324	27.62663365600216	28.510057403877997	21.980507221556607
52-53	21.994472147578197	27.660644809980905	28.387837771363923	21.957045271076975
54-55	22.004918022409438	27.571387383751343	28.386257351952427	22.03743724188679
56-57	22.043824905614766	27.646395757709826	28.3106220063638	21.999157330311604
58-59	21.91155231884844	27.743452404211506	28.447887220443747	21.89710805649631
60-61	22.05445912746103	27.75153765252261	28.37047525074361	21.823527969272742
62-63	21.98944939147294	27.891517647092456	28.299147625418108	21.819885336016494
64-65	21.94141945677957	27.816740116448983	28.29180269451177	21.95003773225967
66-67	21.985503452207105	27.825103518176924	28.14485499449184	22.044538035124127
68-69	21.859752708290156	27.80359168281076	28.24611432731527	22.09054128158381
70-71	21.939199007639907	27.623405900954783	28.266930123176575	22.170464968228735
72-73	22.052104525882164	27.570876033480673	28.223552816289892	22.153466624347278
74-75	21.96038759043329	27.685614534749973	28.211536039035522	22.14246183578121
76-77	22.073790608163417	27.6125776719687	28.127625287357645	22.18600643251024
78-79	22.04903590171762	27.5741005618708	28.094650987840787	22.28221254857079
80-81	22.101278889839094	27.672827081388395	28.01968598045433	22.20620804831818
82-83	22.062440641296856	27.705899697934917	28.09672425675189	22.134935404016336
84-85	22.18495139717424	27.68594257770685	28.027965363919517	22.101140661199395
86-87	22.097376114071896	27.685080946982772	28.096422187712527	22.12112075123281
88-89	22.12574376737386	27.686363038360472	28.022411829181326	22.165481365084343
90-91	22.219320547528625	27.72351874239224	27.957549966717988	22.099610743361147
92-93	22.24239150364103	27.67040672889653	28.016586513939817	22.07061525352262
94-95	22.08555492338601	27.812690420489172	28.00466454001991	22.097090116104912
96-97	22.232963555196772	27.877926262260644	28.04479767243312	21.84431251010946
98-99	23.301670096097137	29.772120322035722	28.251396763613574	18.674812818253564
100	22.167797749069265	30.65798067251606	22.217840818817137	24.95638075959754
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2425.0
1	2381.5
2	3066.5
3	3981.5
4	4603.0
5	5683.0
6	7037.0
7	10383.0
8	14017.0
9	15230.5
10	14833.0
11	14308.5
12	15012.5
13	17274.5
14	22191.0
15	27865.5
16	31559.5
17	32908.0
18	32545.5
19	31340.0
20	30290.5
21	30798.5
22	34615.5
23	42396.0
24	53607.0
25	69063.5
26	88990.0
27	110502.0
28	133115.5
29	158832.5
30	185187.5
31	212042.5
32	240556.5
33	269427.5
34	295058.5
35	316274.0
36	339814.0
37	365386.0
38	385558.0
39	398689.5
40	411332.0
41	424269.5
42	435509.0
43	451385.5
44	462443.0
45	463992.0
46	461906.5
47	457831.5
48	454687.5
49	448711.5
50	438603.0
51	422576.0
52	402358.5
53	380149.5
54	356007.5
55	328268.5
56	298750.0
57	269613.5
58	239116.5
59	205210.0
60	168822.0
61	135548.0
62	106589.5
63	81351.5
64	60270.5
65	44296.0
66	32425.5
67	24212.0
68	17858.5
69	12838.0
70	9541.5
71	7032.0
72	4899.0
73	3231.0
74	2105.0
75	1358.5
76	872.5
77	525.0
78	309.5
79	188.5
80	126.0
81	76.5
82	49.0
83	36.0
84	28.0
85	18.5
86	11.5
87	10.0
88	6.5
89	3.5
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005648222393433337
3	0.0
4	0.0028692247941083087
5	0.0015970213476640585
6	0.0
7	0.0
8	0.0
9	7.218175582662411E-5
10-11	2.8421566356733247E-4
12-13	1.3534079217492022E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.05293718666714E-4
24-25	0.0012678139134320418
26-27	0.0
28-29	0.0020038708104488504
30-31	0.0028724559118665487
32-33	5.066322687481533E-4
34-35	2.7196912171379712E-5
36-37	9.639617445415893E-4
38-39	0.001964149700181684
40-41	0.0014918999244547782
42-43	0.0
44-45	0.0
46-47	0.0013133090930436961
48-49	4.565082904493995E-4
50-51	0.0015680767245255395
52-53	0.002306952411003591
54-55	0.0017072178870397225
56-57	0.0016797120278531006
58-59	0.0017398909661847817
60-61	7.341971048062941E-4
62-63	5.963934755736701E-4
64-65	0.002839581562835199
66-67	0.002246646230025051
68-69	1.009241676493737E-4
70-71	0.002769148791149475
72-73	0.009342747234053744
74-75	0.011560165770602825
76-77	0.004514735836803938
78-79	0.004321896311232545
80-81	2.761565529374321E-4
82-83	4.8215529847154085E-5
84-85	5.402261873023616E-5
86-87	0.001852461469073864
88-89	0.0041890953230839766
90-91	5.105342924496917E-4
92-93	9.523116497012346E-4
94-95	0.002257203534135819
96-97	0.0024049752106749677
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	277.0
22-23	568.0
24-25	709.0
26-27	1977.0
28-29	5026.0
30-31	16422.0
32-33	20947.0
34-35	29845.0
36-37	47720.0
38-39	53909.0
40-41	54452.0
42-43	61185.0
44-45	72790.0
46-47	74926.0
48-49	71187.0
50-51	65824.0
52-53	62365.0
54-55	67448.0
56-57	69326.0
58-59	72860.0
60-61	71489.0
62-63	71122.0
64-65	77219.0
66-67	83183.0
68-69	85770.0
70-71	89675.0
72-73	77781.0
74-75	77270.0
76-77	80986.0
78-79	82867.0
80-81	83028.0
82-83	78345.0
84-85	77713.0
86-87	80942.0
88-89	84374.0
90-91	84809.0
92-93	85281.0
94-95	101741.0
96-97	342878.0
98-99	773407.0
100-101	7643416.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.88824321610653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.63050270534575	76.08575913233284
2	7.156063789856697	12.149313669266567
3	1.7953771850127986	4.572192454380454
4	0.6455100391019319	2.1918485279089293
5	0.30862987449687607	1.3099523925023626
6	0.15862183375093675	0.8079077281700601
7	0.09358690308596966	0.5561099453702789
8	0.056743470422048706	0.38534828144902544
9	0.03603683467563507	0.27531932280155386
>10	0.11691101442615712	1.5123118525883024
>50	0.0016999403971246644	0.09609292024449477
>100	2.9895961313017387E-4	0.0426013592365888
>500	0.0	0.0
>1k	1.744981494282829E-5	0.015242413748562007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.6090877913312056E-5	0.0	0.0	0.0
3	0.0	3.6090877913312056E-5	0.0	0.0	0.0
4	0.0	8.120447530495213E-5	0.0	2.7068158434984044E-5	0.0
5	0.0	8.120447530495213E-5	0.0	2.7068158434984044E-5	0.0
6	0.0	8.120447530495213E-5	0.0	2.7068158434984044E-5	1.8045438956656028E-5
7	0.0	8.120447530495213E-5	0.0	2.7068158434984044E-5	1.8045438956656028E-5
8	0.0	8.120447530495213E-5	0.0	3.6090877913312056E-5	1.8045438956656028E-5
9	0.0	1.0827263373993618E-4	0.0	6.31590363482961E-5	1.8045438956656028E-5
10-11	2.2556798695820038E-5	1.0827263373993618E-4	0.0	8.571583504411613E-5	1.8045438956656028E-5
12-13	4.511359739164007E-5	1.0827263373993618E-4	0.0	1.308294324357562E-4	1.8045438956656028E-5
14-15	4.511359739164007E-5	1.4887487139241223E-4	0.0	1.6240895060990425E-4	2.2556798695820038E-5
16-17	4.511359739164007E-5	1.7143167008823226E-4	0.0	1.7143167008823226E-4	4.060223765247606E-5
18-19	5.413631686996809E-5	2.030111882623803E-4	0.0	1.9849982852321633E-4	4.511359739164007E-5
20-21	6.76703960874601E-5	2.3007934669736437E-4	0.0	2.1203390774070835E-4	5.8647676609132094E-5
22-23	7.218175582662411E-5	2.526361453931844E-4	0.0	2.706815843498404E-4	6.76703960874601E-5
24-25	7.218175582662411E-5	2.8421566356733247E-4	0.0	3.157951817414805E-4	8.120447530495213E-5
26-27	8.120447530495213E-5	2.8872702330649645E-4	0.0	3.6090877913312055E-4	8.120447530495213E-5
28-29	8.120447530495213E-5	2.9774974278482446E-4	0.0	5.594086076563369E-4	9.022719478328015E-5
30-31	9.022719478328014E-5	3.428633401764645E-4	0.0	0.0014030328788800063	1.0376127400077217E-4
32-33	9.022719478328014E-5	3.473746999156285E-4	0.0	0.0032391562927197573	1.0827263373993618E-4
34-35	9.022719478328014E-5	4.1053373626392465E-4	0.0	0.005706870070042469	1.1729535321826419E-4
36-37	9.924991426160816E-5	4.6015869339472874E-4	0.0	0.009906945987204159	1.1729535321826419E-4
38-39	1.0376127400077217E-4	4.736927726122208E-4	0.0	0.019822914693886647	1.1729535321826419E-4
40-41	1.263180726965922E-4	5.278290894821889E-4	0.0	0.030835143817185988	1.1729535321826419E-4
42-43	1.263180726965922E-4	5.41363168699681E-4	0.0	0.0378277514128902	1.218067129574282E-4
44-45	1.263180726965922E-4	5.77454046612993E-4	0.0	0.04611963061347364	1.353407921749202E-4
46-47	1.263180726965922E-4	6.09033564787141E-4	0.0	0.05531829312162906	1.4436351165324823E-4
48-49	1.308294324357562E-4	6.13544924526305E-4	0.0	0.06492748936604839	1.4436351165324823E-4
50-51	1.4436351165324823E-4	6.36101723222125E-4	0.0	0.07453217425072856	1.4436351165324823E-4
52-53	1.5338623113157624E-4	6.40613082961289E-4	0.0	0.09778823370611903	1.5338623113157624E-4
54-55	1.6240895060990425E-4	6.451244427004531E-4	0.0	0.12766696925860224	1.6240895060990425E-4
56-57	1.6240895060990425E-4	6.49635802439617E-4	0.0	0.17110685218701244	1.6692031034906826E-4
58-59	1.6240895060990425E-4	6.94749399831257E-4	0.0	0.20235704110020153	1.7143167008823226E-4
60-61	1.6240895060990425E-4	7.218175582662412E-4	0.0	0.21628360861500084	1.7594302982739627E-4
62-63	1.6692031034906826E-4	7.398629972228971E-4	0.0	0.2267274064111655	1.894771090448883E-4
64-65	1.7143167008823226E-4	8.481356309628334E-4	0.0	0.23138764102172193	1.894771090448883E-4
66-67	1.8045438956656028E-4	8.706924296586534E-4	0.0	0.23616968234523578	1.894771090448883E-4
68-69	1.8045438956656028E-4	9.022719478328015E-4	0.0	0.2408434510350097	1.894771090448883E-4
70-71	1.8045438956656028E-4	9.203173867894575E-4	0.0	0.2448630725626048	1.894771090448883E-4
72-73	1.9849982852321633E-4	9.248287465286214E-4	0.0	0.24839546723837025	1.9849982852321633E-4
74-75	1.9849982852321633E-4	9.473855452244415E-4	0.0	0.2510661922039553	1.9849982852321633E-4
76-77	1.9849982852321633E-4	9.789650633985894E-4	0.0	0.25325871303718905	1.9849982852321633E-4
78-79	2.0301118826238033E-4	9.924991426160815E-4	0.0	0.255072279652333	1.9849982852321633E-4
80-81	2.0752254800154434E-4	9.970105023552455E-4	0.0	0.25560010874181516	2.0301118826238033E-4
82-83	2.1203390774070835E-4	0.0010331013802685578	0.0	0.2560602674352099	2.1654526747987235E-4
84-85	2.1654526747987235E-4	0.0010691922581818696	0.0	0.25622718774555897	2.1654526747987235E-4
86-87	2.1654526747987235E-4	0.0010917490568776898	0.0	0.25633997173903805	2.1654526747987235E-4
88	2.1654526747987235E-4	0.0011188172153126738	0.0	0.25635350581825556	2.1654526747987235E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12735	0.0	44.02695	1
GTATCAA	22535	0.0	32.8277	1
ATCAACG	25425	0.0	30.237318	3
TCAACGC	25495	0.0	30.205456	4
CAACGCA	26015	0.0	29.634228	5
AACGCAG	27060	0.0	28.53776	6
TATCAAC	28215	0.0	27.525457	2
ACGCAGA	30445	0.0	25.307846	7
CGCAGAG	31735	0.0	24.265442	8
TACATGG	28145	0.0	20.599144	2
GTACATG	27760	0.0	20.338108	1
GCAGAGT	37845	0.0	20.313463	9
ACATGGG	28185	0.0	20.04573	3
GAGTACT	22975	0.0	18.919373	12-13
AGAGTAC	34110	0.0	18.705072	10-11
CATGGGG	21720	0.0	18.30657	4
CAGAGTA	37440	0.0	18.11264	10-11
GTGGTAT	6845	0.0	16.977758	1
GTACTTT	26335	0.0	16.33293	14-15
TATGCCG	4510	0.0	15.899203	42-43
>>END_MODULE