FastQCFastQC Report
Fri 27 May 2016
SRR2138993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9601563
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110600.047.067451
GTATCAA199000.036.2319761
ATCAACG243600.030.2890593
TCAACGC244250.030.226144
CAACGCA251800.029.4220055
TATCAAC263600.028.4670962
AACGCAG260550.028.4499196
ACGCAGA295650.025.043087
CGCAGAG306000.024.1536858
CTTATAC88300.024.0709461
GTGGTAT57850.021.2828731
TACATGG265950.020.7758732
TATACAC130500.020.6230413
GCAGAGT362550.020.36249
GTACATG267850.019.9349691
TGGTATC57750.019.8236052
ACATGGG271800.019.7400073
GAGTACT214350.019.2254912-13
AGAGTAC324450.018.85911810-11
TATGCCG66400.018.5753242-43
Produced by FastQC (version 0.11.5)