##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9601563
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.678927899551354	18.0	18.0	25.0	18.0	33.0
2	30.553987199792367	32.0	27.0	32.0	27.0	33.0
3	31.564861158542623	33.0	31.0	33.0	29.0	33.0
4	32.5068587270635	33.0	33.0	33.0	31.0	34.0
5	32.902573987172715	33.0	33.0	34.0	32.0	34.0
6	37.18404638911394	38.0	38.0	38.0	36.0	38.0
7	37.298235610181386	38.0	38.0	38.0	36.0	38.0
8	37.36665238774145	38.0	38.0	38.0	37.0	38.0
9	37.44626015576839	38.0	38.0	38.0	37.0	38.0
10-11	37.464927012404125	38.0	38.0	38.0	37.0	38.0
12-13	37.488408033150435	38.0	38.0	38.0	37.0	38.0
14-15	37.47849240795483	38.0	38.0	38.0	37.0	38.0
16-17	37.421834497154265	38.0	38.0	38.0	37.0	38.0
18-19	37.45204004806301	38.0	38.0	38.0	37.0	38.0
20-21	37.47485800248643	38.0	38.0	38.0	37.0	38.0
22-23	37.48060087410697	38.0	38.0	38.0	37.0	38.0
24-25	37.481923552068736	38.0	38.0	38.0	37.0	38.0
26-27	37.451013179939956	38.0	38.0	38.0	37.0	38.0
28-29	37.42627123445021	38.0	38.0	38.0	37.0	38.0
30-31	37.33026072171166	38.0	38.0	38.0	37.0	38.0
32-33	37.392377662533576	38.0	38.0	38.0	37.0	38.0
34-35	37.39795310832897	38.0	38.0	38.0	37.0	38.0
36-37	37.37547664711066	38.0	38.0	38.0	37.0	38.0
38-39	37.347723274154674	38.0	38.0	38.0	37.0	38.0
40-41	37.34831371967127	38.0	38.0	38.0	37.0	38.0
42-43	37.33007682729819	38.0	38.0	38.0	37.0	38.0
44-45	37.29527567383394	38.0	38.0	38.0	37.0	38.0
46-47	37.26601894718213	38.0	38.0	38.0	37.0	38.0
48-49	37.23617265383511	38.0	38.0	38.0	37.0	38.0
50-51	37.190353829296996	38.0	38.0	38.0	37.0	38.0
52-53	37.16898626407681	38.0	38.0	38.0	37.0	38.0
54-55	37.151927323992766	38.0	38.0	38.0	36.5	38.0
56-57	37.143461805992914	38.0	38.0	38.0	36.0	38.0
58-59	37.130678031980004	38.0	38.0	38.0	36.0	38.0
60-61	37.1288380594345	38.0	38.0	38.0	36.0	38.0
62-63	37.13686904647062	38.0	38.0	38.0	36.0	38.0
64-65	37.13990987220556	38.0	38.0	38.0	36.0	38.0
66-67	37.12028857742288	38.0	38.0	38.0	36.0	38.0
68-69	37.117639766067406	38.0	38.0	38.0	36.0	38.0
70-71	37.0998366097443	38.0	38.0	38.0	36.0	38.0
72-73	37.088728417593174	38.0	38.0	38.0	36.0	38.0
74-75	37.076743235030975	38.0	38.0	38.0	36.0	38.0
76-77	37.078737929995754	38.0	38.0	38.0	36.0	38.0
78-79	37.06275996963542	38.0	38.0	38.0	36.0	38.0
80-81	37.05802141439772	38.0	38.0	38.0	36.0	38.0
82-83	37.01035393846661	38.0	38.0	38.0	36.0	38.0
84-85	36.99688479157868	38.0	38.0	38.0	36.0	38.0
86-87	36.98841586483748	38.0	38.0	38.0	36.0	38.0
88-89	36.991054447839645	38.0	38.0	38.0	36.0	38.0
90-91	36.98059362786918	38.0	38.0	38.0	36.0	38.0
92-93	36.96309361279508	38.0	38.0	38.0	36.0	38.0
94-95	36.93520293622421	38.0	38.0	38.0	35.5	38.0
96-97	36.9016474769479	38.0	38.0	38.0	35.0	38.0
98-99	36.907266467868006	38.0	38.0	38.0	35.0	38.0
100	35.362376812142756	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	34.0
20	145.0
21	350.0
22	858.0
23	1964.0
24	4138.0
25	8032.0
26	13987.0
27	22678.0
28	35156.0
29	51452.0
30	71372.0
31	94119.0
32	122315.0
33	163374.0
34	243449.0
35	444505.0
36	1485745.0
37	6837880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.709461886569926	17.723645618947664	19.201457096099876	23.365435398382534
2	14.299323056643034	20.82617649022477	42.29388317886426	22.580617274267933
3	18.3714880587671	25.745277097072634	30.48415138243638	25.399083461723887
4	12.399304924851561	18.103626772474957	38.762500437442405	30.734567865231078
5	13.300784028719043	37.41486948720368	35.867527704715776	13.4168187793615
6	29.883301291675117	37.83657931526357	18.76537184622962	13.514747546831698
7	26.309560224725914	32.53977503454386	23.15668813504635	17.993976605683887
8	23.954964415689403	37.1760826857044	21.288294416232024	17.580658482374172
9	25.218256472810424	17.069580863419105	21.503386745553946	36.208775918216524
10-11	24.149709317463657	26.850531268942273	29.016584839515332	19.983174574078742
12-13	24.875650441042822	24.262974086924608	29.140841875519836	21.720533596512734
14-15	22.04561903098485	25.651958957098962	26.678182500078375	25.62423951183781
16-17	21.739616768644854	28.816579134043074	27.360232912079002	22.08357118523307
18-19	21.770174293497842	27.86579643335153	29.092544620079043	21.27148465307159
20-21	22.47545604324015	27.03116519832896	29.009127478389455	21.484251280041438
22-23	22.18874917444278	27.091707598646863	28.878754508211617	21.84078871869874
24-25	21.988524348885544	27.269061270864924	28.974149369913093	21.768265010336442
26-27	21.987685502759504	27.408707045499327	28.895124998912742	21.708482452828427
28-29	21.90447303005692	27.382908375793313	28.947848140095818	21.76477045405395
30-31	22.276552322149044	27.21516205519501	28.80958998823181	21.69869563442414
32-33	21.959482130024593	27.401864867914895	28.829464898433248	21.80918810362726
34-35	22.068793716975538	27.23004166580533	28.912850354001034	21.78831426321809
36-37	21.936485081210304	27.352803336682776	28.905613792977437	21.805097789129487
38-39	21.80438748455426	27.29537700532178	29.058244903835345	21.841990606288615
40-41	22.095333563858944	27.126684923121335	28.95301620454342	21.8249653084763
42-43	22.050809015323985	27.195444106053152	28.8383370253593	21.915409853263558
44-45	21.996173741693557	27.3786826368376	28.716462308469843	21.908681312999
46-47	22.184199323389915	27.18521699950826	28.442364438489975	22.188219238611847
48-49	22.04737248809679	27.271618469481623	28.483040193825733	22.19796884859585
50-51	21.91389605601846	27.418982268636228	28.519771531863586	22.147350143481727
52-53	22.11983236452576	27.38646620820363	28.491859766427375	22.001841660843233
54-55	22.11069403498016	27.285942775862456	28.407375784385465	22.195987404771923
56-57	22.190499032130777	27.458043962810624	28.252836972207636	22.09862003285096
58-59	21.97660418679629	27.654768085095487	28.499273997345803	21.86935373076242
60-61	22.149081839793638	27.780705509051923	28.21558979307105	21.854622858083385
62-63	22.03592564113046	27.97980958323123	28.137738480571105	21.8465262950672
64-65	21.958109440537775	27.92314105960924	28.206531334674423	21.912218165178558
66-67	22.034047976435616	27.94821827944331	28.03011319135856	21.987620552762515
68-69	21.978074943007023	27.832649277036737	28.13646151536932	22.05281426458692
70-71	22.024058205958866	27.511489706645627	28.223127415390053	22.241324672005458
72-73	22.151839989662903	27.41909215825107	28.175279311119926	22.2537885409661
74-75	22.026193736259497	27.54437338488834	28.16562487946928	22.26380799938288
76-77	22.191852567443522	27.495925773227953	28.018938834090484	22.293282825238038
78-79	22.151218268873556	27.54846431639521	27.97063345051806	22.329683964213174
80-81	22.19190140191461	27.57147616215511	27.94104944482023	22.295572991110046
82-83	22.22939352851663	27.513619515698938	28.046686848166967	22.210300107617464
84-85	22.35999644535786	27.550485648888145	27.870132623295035	22.219385282458955
86-87	22.18553888859029	27.594659709666725	27.97587032066723	22.243931081075758
88-89	22.251929039184766	27.58693539798525	27.91532328257086	22.245812280259123
90-91	22.289567045229383	27.6239554095768	27.89871313878049	22.187764406413326
92-93	22.348008681716728	27.579738451747442	27.922234490405355	22.15001837613047
94-95	22.232735008979553	27.692779795167695	27.88815653695732	22.186328658895434
96-97	22.329570612468693	27.741529355795098	27.948005673526232	21.980894358209984
98-99	23.37194272802013	29.64556146204912	28.188261125419235	18.79423468451152
100	22.251070939966926	30.57355802367087	22.155652432439364	25.01971860392284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1844.0
1	1867.5
2	2658.0
3	3631.5
4	4221.5
5	5245.0
6	6492.0
7	9359.0
8	12527.5
9	13766.0
10	13601.5
11	13240.0
12	14122.5
13	16425.0
14	20971.5
15	26195.5
16	29512.5
17	30759.0
18	30236.0
19	28427.0
20	26876.5
21	26803.0
22	29231.5
23	35022.5
24	44521.5
25	58722.0
26	76878.0
27	96422.0
28	116843.0
29	139291.0
30	160425.0
31	179884.5
32	201187.0
33	223248.0
34	245503.0
35	266266.5
36	287809.0
37	308021.5
38	321207.5
39	333029.5
40	348618.5
41	361626.0
42	371161.5
43	390496.5
44	403269.5
45	403794.0
46	401287.0
47	396467.5
48	394582.0
49	391115.0
50	384114.5
51	371737.5
52	355951.5
53	336531.0
54	315427.0
55	294095.0
56	270986.0
57	245773.0
58	218439.0
59	188770.5
60	156617.5
61	125352.0
62	97974.0
63	74800.0
64	55567.5
65	41129.0
66	30618.0
67	22770.0
68	16577.5
69	12010.5
70	8725.5
71	6132.5
72	4354.5
73	3078.0
74	2091.5
75	1316.5
76	779.5
77	430.0
78	274.5
79	185.5
80	109.0
81	57.0
82	33.0
83	25.5
84	21.5
85	14.0
86	8.0
87	6.0
88	6.5
89	4.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006373962239272919
3	0.0
4	0.00311407632278203
5	0.0013435312563173309
6	0.0
7	0.0
8	0.0
9	1.5622456468806172E-4
10-11	2.3954433252169465E-4
12-13	1.0414970979204115E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.999406841209152E-4
24-25	0.0013697014233176824
26-27	0.0
28-29	0.0020370163909487703
30-31	0.003050011746455495
32-33	6.214342598274083E-4
34-35	3.140045889677314E-5
36-37	9.818247438631883E-4
38-39	0.0021417705447361267
40-41	0.0015642690822799173
42-43	0.0
44-45	0.0
46-47	0.001361565649211521
48-49	4.5717515174677377E-4
50-51	0.0016221340631329098
52-53	0.0025256299047815467
54-55	0.0019033877027059345
56-57	0.0017765415730001883
58-59	0.0019917062874622933
60-61	9.408452077255861E-4
62-63	6.737097117310331E-4
64-65	0.0031122486255194034
66-67	0.0024657796203372395
68-69	1.171277762723605E-4
70-71	0.0028620331493215533
72-73	0.009669176130616824
74-75	0.011798565347481006
76-77	0.0048008422257287955
78-79	0.004512482378480413
80-81	3.341239696452086E-4
82-83	3.1218794863359396E-5
84-85	7.557702586894528E-5
86-87	0.0019186273301474803
88-89	0.004327708167571425
90-91	5.307923999072797E-4
92-93	0.0010849740570284586
94-95	0.002218265435265271
96-97	0.002552499077482617
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	72.0
20-21	239.0
22-23	498.0
24-25	663.0
26-27	1804.0
28-29	4702.0
30-31	14757.0
32-33	18872.0
34-35	26926.0
36-37	42647.0
38-39	49072.0
40-41	48693.0
42-43	55276.0
44-45	65892.0
46-47	68318.0
48-49	64174.0
50-51	58350.0
52-53	55705.0
54-55	60093.0
56-57	61845.0
58-59	64542.0
60-61	65240.0
62-63	64730.0
64-65	70603.0
66-67	78755.0
68-69	81866.0
70-71	92314.0
72-73	70454.0
74-75	69148.0
76-77	71355.0
78-79	74331.0
80-81	73904.0
82-83	69720.0
84-85	68286.0
86-87	70993.0
88-89	73585.0
90-91	74820.0
92-93	74587.0
94-95	88927.0
96-97	295059.0
98-99	657558.0
100-101	6552188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.0562441866546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24486332420159	75.01588023983703
2	7.309063575802615	12.287448654068946
3	1.8905328619067858	4.767332756499952
4	0.7038593238251196	2.3665508478599135
5	0.3301217312079274	1.3874396424867348
6	0.17165158293167412	0.8657032421958356
7	0.10984841632643555	0.6463411714376514
8	0.06024540409232331	0.4051201918006406
9	0.03995088265909308	0.3022309033438601
>10	0.13721489993100974	1.7187780742010046
>50	0.0022492636113420152	0.12517902922041846
>100	3.362623561557272E-4	0.0489471590857647
>500	0.0	0.0
>1k	6.247114787272461E-5	0.06304808796217837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.2074854896020575E-5	0.0	0.0	0.0
3	0.0	5.2074854896020575E-5	0.0	0.0	0.0
4	0.0	9.373473881283703E-5	0.0	0.0	0.0
5	0.0	9.373473881283703E-5	0.0	0.0	0.0
6	0.0	9.373473881283703E-5	0.0	0.0	0.0
7	0.0	1.0414970979204115E-4	0.0	1.0414970979204115E-5	0.0
8	0.0	1.0414970979204115E-4	0.0	1.0414970979204115E-5	0.0
9	0.0	2.4995930350089874E-4	0.0	2.082994195840823E-5	0.0
10-11	4.165988391681646E-5	2.603742744801029E-4	0.0	2.082994195840823E-5	0.0
12-13	5.2074854896020575E-5	2.603742744801029E-4	0.0	2.082994195840823E-5	5.207485489602057E-6
14-15	6.248982587522468E-5	3.541090132929399E-4	0.0	2.082994195840823E-5	2.082994195840823E-5
16-17	8.852725332323498E-5	3.853539262305523E-4	0.0	3.124491293761234E-5	3.124491293761234E-5
18-19	1.0414970979204115E-4	4.165988391681646E-4	0.0	3.64523984272144E-5	3.124491293761234E-5
20-21	1.0414970979204115E-4	4.53051237595379E-4	0.0	5.2074854896020575E-5	3.124491293761234E-5
22-23	1.1456468077124527E-4	4.947111215121954E-4	0.0	8.852725332323498E-5	3.64523984272144E-5
24-25	1.3018713724005144E-4	5.36371005429012E-4	0.0	9.373473881283703E-5	4.165988391681646E-5
26-27	1.3539462272965349E-4	5.624084328770222E-4	0.0	1.0935719528164321E-4	4.165988391681646E-5
28-29	1.3539462272965349E-4	5.624084328770222E-4	0.0	2.343368470320926E-4	4.165988391681646E-5
30-31	1.3539462272965349E-4	6.457282007106552E-4	0.0	8.748575622531457E-4	4.165988391681646E-5
32-33	1.3539462272965349E-4	6.613506571794612E-4	0.0	0.0021402765362264456	5.2074854896020575E-5
34-35	1.4060210821925553E-4	7.915377944195127E-4	0.0	0.003962896457587166	6.248982587522468E-5
36-37	1.458095937088576E-4	9.165174461699621E-4	0.0	0.007082180265858798	6.248982587522468E-5
38-39	1.458095937088576E-4	9.790072720451868E-4	0.0	0.0159505280546511	6.769731136482674E-5
40-41	1.6663953566726584E-4	0.001072742010858024	0.0	0.02520943725516356	7.29047968544288E-5
42-43	1.6663953566726584E-4	0.0010831569818372279	0.0	0.0308803889533402	7.29047968544288E-5
44-45	1.6663953566726584E-4	0.0011977216626084734	0.0	0.03776989225608372	7.29047968544288E-5
46-47	1.6663953566726584E-4	0.0012914564014213103	0.0	0.045461348324225964	7.29047968544288E-5
48-49	1.6663953566726584E-4	0.0012914564014213103	0.0	0.052730998067710436	7.29047968544288E-5
50-51	1.6663953566726584E-4	0.001452888451598974	0.0	0.06027664454214382	7.29047968544288E-5
52-53	1.7705450664646995E-4	0.0014737183935573822	0.0	0.08210642371455563	7.29047968544288E-5
54-55	1.82261992136072E-4	0.0015049633064949945	0.0	0.11021643038742754	7.29047968544288E-5
56-57	1.8746947762567407E-4	0.0015622456468806173	0.0	0.1536781042836463	7.29047968544288E-5
58-59	1.8746947762567407E-4	0.0015882830743286275	0.0	0.18489177230832105	7.29047968544288E-5
60-61	1.8746947762567407E-4	0.0016143205017766379	0.0	0.19684815899244737	7.29047968544288E-5
62-63	1.8746947762567407E-4	0.0016559803856934542	0.0	0.20573733672319808	7.29047968544288E-5
64-65	1.8746947762567407E-4	0.0018694872907671386	0.0	0.2097627230066605	7.29047968544288E-5
66-67	1.8746947762567407E-4	0.001895524718215149	0.0	0.21420991561478064	7.29047968544288E-5
68-69	1.9788444860487818E-4	0.001968429515069578	0.0	0.21815718961589897	7.29047968544288E-5
70-71	1.9788444860487818E-4	0.0020257118554552	0.0	0.2222190282977886	7.29047968544288E-5
72-73	1.9788444860487818E-4	0.002046541797413609	0.0	0.22555702649662351	7.29047968544288E-5
74-75	1.9788444860487818E-4	0.0020725792248616186	0.0	0.22811910935750773	7.29047968544288E-5
76-77	1.9788444860487818E-4	0.002145484021716048	0.0	0.23070722964583995	7.29047968544288E-5
78-79	1.9788444860487818E-4	0.002155898992695252	0.0	0.23236841751702303	7.29047968544288E-5
80-81	1.9788444860487818E-4	0.002155898992695252	0.0	0.23292561846441043	7.811228234403086E-5
82-83	1.9788444860487818E-4	0.002171521449164058	0.0	0.2333474247890682	1.0414970979204115E-4
84-85	1.9788444860487818E-4	0.002197558876612068	0.0	0.23346719695532903	1.0414970979204115E-4
86-87	1.9788444860487818E-4	0.0022183888185704765	0.0	0.23356093169414188	1.1456468077124527E-4
88	1.9788444860487818E-4	0.0023850283542377424	0.0	0.23356613917963148	1.1456468077124527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11060	0.0	47.06745	1
GTATCAA	19900	0.0	36.231976	1
ATCAACG	24360	0.0	30.289059	3
TCAACGC	24425	0.0	30.22614	4
CAACGCA	25180	0.0	29.422005	5
TATCAAC	26360	0.0	28.467096	2
AACGCAG	26055	0.0	28.449919	6
ACGCAGA	29565	0.0	25.04308	7
CGCAGAG	30600	0.0	24.153685	8
CTTATAC	8830	0.0	24.070946	1
GTGGTAT	5785	0.0	21.282873	1
TACATGG	26595	0.0	20.775873	2
TATACAC	13050	0.0	20.623041	3
GCAGAGT	36255	0.0	20.3624	9
GTACATG	26785	0.0	19.934969	1
TGGTATC	5775	0.0	19.823605	2
ACATGGG	27180	0.0	19.740007	3
GAGTACT	21435	0.0	19.22549	12-13
AGAGTAC	32445	0.0	18.859118	10-11
TATGCCG	6640	0.0	18.57532	42-43
>>END_MODULE