FastQCFastQC Report
Fri 27 May 2016
SRR2138992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7829887
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100150.051.7772751
GTATCAA183400.036.7894331
ATCAACG231200.029.9649053
TCAACGC237350.029.2433784
CAACGCA240050.028.860185
TATCAAC241450.028.7663252
AACGCAG241950.028.651196
ACGCAGA281000.024.6849447
CGCAGAG289900.023.9122318
TGGTATC46100.021.8042262
TACATGG251850.021.3090522
GTACATG246800.021.1857171
ACATGGG249950.020.8999963
GCAGAGT334750.020.7342289
GTGGTAT50650.020.612071
GAGTACT199950.019.70761312-13
ACTTCGC13000.019.2458486
CAGAGTA326250.018.88110210-11
CGAGACC79150.018.8496286-87
AGAGTAC304800.018.70967110-11
Produced by FastQC (version 0.11.5)