##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7829887
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.142118015240833	18.0	18.0	28.0	18.0	33.0
2	30.780799518562656	32.0	28.0	33.0	27.0	33.0
3	31.654937932054448	33.0	31.0	33.0	29.0	33.0
4	32.51775842997479	33.0	33.0	33.0	31.0	34.0
5	32.89993329405648	33.0	33.0	34.0	32.0	34.0
6	37.16721939920716	38.0	38.0	38.0	36.0	38.0
7	37.286743218644155	38.0	38.0	38.0	36.0	38.0
8	37.360582087583126	38.0	38.0	38.0	37.0	38.0
9	37.441013005679395	38.0	38.0	38.0	37.0	38.0
10-11	37.4587366331085	38.0	38.0	38.0	37.0	38.0
12-13	37.48121760888759	38.0	38.0	38.0	37.0	38.0
14-15	37.47149320290318	38.0	38.0	38.0	37.0	38.0
16-17	37.41368853726752	38.0	38.0	38.0	37.0	38.0
18-19	37.44309719923161	38.0	38.0	38.0	37.0	38.0
20-21	37.46682592437837	38.0	38.0	38.0	37.0	38.0
22-23	37.472145801311065	38.0	38.0	38.0	37.0	38.0
24-25	37.47427044047896	38.0	38.0	38.0	37.0	38.0
26-27	37.44514173370103	38.0	38.0	38.0	37.0	38.0
28-29	37.4184029753252	38.0	38.0	38.0	37.0	38.0
30-31	37.320784882947294	38.0	38.0	38.0	37.0	38.0
32-33	37.38421839785218	38.0	38.0	38.0	37.0	38.0
34-35	37.39122488083517	38.0	38.0	38.0	37.0	38.0
36-37	37.3703507540329	38.0	38.0	38.0	37.0	38.0
38-39	37.34425009244404	38.0	38.0	38.0	37.0	38.0
40-41	37.33757482303504	38.0	38.0	38.0	37.0	38.0
42-43	37.315792359759094	38.0	38.0	38.0	37.0	38.0
44-45	37.276781964908054	38.0	38.0	38.0	37.0	38.0
46-47	37.24552183409597	38.0	38.0	38.0	37.0	38.0
48-49	37.21083982721905	38.0	38.0	38.0	37.0	38.0
50-51	37.164874490785174	38.0	38.0	38.0	37.0	38.0
52-53	37.14180240700432	38.0	38.0	38.0	36.5	38.0
54-55	37.12988530198401	38.0	38.0	38.0	36.0	38.0
56-57	37.121484940224384	38.0	38.0	38.0	36.0	38.0
58-59	37.11125558334877	38.0	38.0	38.0	36.0	38.0
60-61	37.11174301035831	38.0	38.0	38.0	36.0	38.0
62-63	37.121145049490764	38.0	38.0	38.0	36.0	38.0
64-65	37.12751070765796	38.0	38.0	38.0	36.0	38.0
66-67	37.108998381761026	38.0	38.0	38.0	36.0	38.0
68-69	37.110668193912005	38.0	38.0	38.0	36.0	38.0
70-71	37.092852429692584	38.0	38.0	38.0	36.0	38.0
72-73	37.08586878295358	38.0	38.0	38.0	36.0	38.0
74-75	37.07583315249222	38.0	38.0	38.0	36.0	38.0
76-77	37.07650900079055	38.0	38.0	38.0	36.0	38.0
78-79	37.05983196846568	38.0	38.0	38.0	36.0	38.0
80-81	37.05425106296593	38.0	38.0	38.0	36.0	38.0
82-83	37.00497165287233	38.0	38.0	38.0	36.0	38.0
84-85	36.99020295284194	38.0	38.0	38.0	36.0	38.0
86-87	36.98068112056792	38.0	38.0	38.0	36.0	38.0
88-89	36.983259822216354	38.0	38.0	38.0	36.0	38.0
90-91	36.96956140749597	38.0	38.0	38.0	36.0	38.0
92-93	36.95210853138976	38.0	38.0	38.0	36.0	38.0
94-95	36.91960023446911	38.0	38.0	38.0	35.0	38.0
96-97	36.88507721289977	38.0	38.0	38.0	35.0	38.0
98-99	36.88820720754647	38.0	38.0	38.0	35.0	38.0
100	35.34305700297931	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	38.0
20	86.0
21	248.0
22	632.0
23	1581.0
24	3343.0
25	6491.0
26	11394.0
27	19221.0
28	30007.0
29	43910.0
30	60221.0
31	78755.0
32	101597.0
33	134957.0
34	199759.0
35	363942.0
36	1200648.0
37	5573050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57388056813591	18.071895545874415	18.03295500944011	23.32126887654956
2	14.507193093710423	20.756508972288373	42.091288140767126	22.645009793234077
3	18.392487656590703	25.8656478695031	30.41702900693203	25.32483546697417
4	12.49534780772863	17.902210599156383	38.54149397890734	31.060947614207645
5	13.299647345692666	37.402046856073824	35.71147227426012	13.586833523973391
6	30.03372845610671	37.689381724155155	18.570740548362956	13.706149271375182
7	26.345629253653343	32.57812022063665	23.156694343098437	17.919556182611576
8	24.210630370527696	36.943955896170664	21.085681057721523	17.75973267558012
9	25.482782292182186	16.81501939686138	21.36481106020211	36.33738725075432
10-11	24.34802826715764	26.697813652957446	28.74496287547268	20.209195204412236
12-13	25.023865291516717	24.285399831198042	28.995315448738047	21.6954194285472
14-15	22.19646975748181	25.56400877815989	26.670333811969442	25.56918765238885
16-17	21.789292744582394	28.669226005432773	27.341275806406912	22.20020544357792
18-19	21.80023159976638	27.669690507666328	29.23420861629293	21.295869276274356
20-21	22.61782130798151	26.771656409396776	29.155053202104135	21.455469080517574
22-23	22.363652828137422	26.787009977754693	28.97528672586061	21.874050468247276
24-25	22.154895895501774	26.873358872887547	29.0808794062357	21.890865825374973
26-27	22.050269985995804	27.00819289433086	29.075430654840105	21.866106464833223
28-29	21.930888725046096	26.996618932190806	29.122174616946484	21.95031772581661
30-31	22.267344435576167	26.936691024519714	29.004795903921295	21.79116863598282
32-33	22.10555079560618	27.021516622358142	29.00862325182888	21.8643093302068
34-35	22.040098201616605	27.00356343283462	29.113861961100845	21.842476404447925
36-37	22.16408581070917	26.987473832011418	28.934980983816516	21.9134593734629
38-39	21.90944711230451	27.05012630978786	29.082387571922315	21.958039005985317
40-41	22.198256782218078	26.892326592169113	28.990519163730404	21.91889746188241
42-43	22.093224905495305	27.04268306853863	28.789662332333254	22.074429693632812
44-45	22.114188755817644	27.1814457129799	28.662053835753436	22.04231169544902
46-47	22.24696325342309	27.07415289004994	28.41955636226893	22.259327494258034
48-49	22.202207135391102	27.18855734018825	28.35533527204842	22.253900252372226
50-51	22.075840198468654	27.370361164519412	28.367318099910865	22.186480537101072
52-53	22.28127344528663	27.240779610113474	28.291420376946746	22.186526567653146
54-55	22.25116019724805	27.17528912255503	28.215457247376296	22.358093432820624
56-57	22.27467966513242	27.358183228048365	28.121704540168174	22.24543256665104
58-59	22.109771020262396	27.378893499921098	28.238889457962244	22.27244602185426
60-61	22.322106801674572	27.427155249505336	28.230598572063204	22.02013937675689
62-63	22.068486757450234	27.526619541483143	28.20812413807908	22.196769562987544
64-65	22.067620405592358	27.499492779799983	28.237227771207284	22.195659043400376
66-67	22.160987077046627	27.466409276099007	28.182513259758952	22.190090387095413
68-69	22.125440754646498	27.612894563818568	28.094664953078507	22.166999728456428
70-71	22.099652407079343	27.33665032044687	28.02147163624481	22.542225636228977
72-73	22.18804450036703	27.296134184476216	27.954172494415303	22.561648820741446
74-75	22.084563680844198	27.478229145808484	28.046723111690415	22.390484061656895
76-77	22.239075967785638	27.219967043185246	27.96372601797883	22.577230971050284
78-79	22.236447580095742	27.28910950865679	27.7130321993949	22.761410711852566
80-81	22.35595371846272	27.51169381299792	27.707394179378603	22.42495828916076
82-83	22.45794992277154	27.22612383836214	27.78385723926606	22.532068999600266
84-85	22.33517086200991	27.252560973081586	27.726395528047632	22.68587263686087
86-87	22.261381178003404	27.47144915651807	27.71481100589955	22.55235865957898
88-89	22.528047355737606	27.48129585440348	27.63875179124149	22.351904998617425
90-91	22.493080332424075	27.54663337249546	27.638328116666642	22.32195817841382
92-93	22.397469295297782	27.268952126870726	27.63302693035244	22.700551647479056
94-95	22.472402164342625	27.606048803782006	27.616988844648095	22.304560187227278
96-97	22.756660063111138	27.440001139897834	27.719902064326973	22.08343673266406
98-99	23.546887200363425	29.46378143233176	27.980279727009123	19.009051640295695
100	22.41532453152984	30.373031359607968	21.998391041135967	25.213253067726228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1727.0
1	1725.0
2	2410.0
3	3256.0
4	3907.0
5	5130.5
6	6391.0
7	8865.0
8	11482.5
9	12361.5
10	12157.0
11	11816.5
12	12560.0
13	14911.5
14	20005.0
15	26162.0
16	30415.5
17	32230.5
18	31707.0
19	29558.0
20	26955.0
21	24960.0
22	25168.5
23	28658.0
24	35532.5
25	45957.5
26	59412.0
27	74595.0
28	90445.5
29	108020.0
30	124620.0
31	140026.0
32	157119.0
33	174624.0
34	192013.0
35	208039.0
36	225072.5
37	240995.5
38	252587.0
39	263502.0
40	276486.5
41	288975.5
42	295984.5
43	300990.5
44	306788.5
45	314803.5
46	325611.0
47	329069.5
48	326357.0
49	323599.5
50	317731.0
51	308691.5
52	297095.0
53	284451.0
54	272630.5
55	259627.0
56	239958.0
57	213942.0
58	188230.5
59	161295.0
60	132875.0
61	106216.0
62	82763.0
63	64006.0
64	47703.5
65	34863.0
66	25988.5
67	19697.0
68	14877.0
69	10996.0
70	8097.5
71	5824.0
72	4100.5
73	2865.5
74	1987.0
75	1306.5
76	852.5
77	509.5
78	289.0
79	175.0
80	118.0
81	82.0
82	51.0
83	30.0
84	18.5
85	14.0
86	10.0
87	6.5
88	4.0
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00641133135126982
3	0.0
4	0.003129036217253199
5	0.0015709038968250754
6	0.0
7	0.0
8	0.0
9	1.5325891676342201E-4
10-11	2.937462571298922E-4
12-13	1.9157364595427752E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.428147011335696E-4
24-25	0.0012900533628657628
26-27	0.0
28-29	0.00206999866600086
30-31	0.00296050475646971
32-33	5.572696757990783E-4
34-35	6.420252249142768E-6
36-37	0.0010500907458788125
38-39	0.0021818935210063907
40-41	0.0015232223051821455
42-43	0.0
44-45	0.0
46-47	0.0013287562111048143
48-49	4.620218383661461E-4
50-51	0.0017469576967785966
52-53	0.0025525878930348907
54-55	0.0019747648287414244
56-57	0.001906186317807503
58-59	0.0021939603524372374
60-61	9.398538116571947E-4
62-63	7.155220943752598E-4
64-65	0.003322447017573624
66-67	0.0026735545035455255
68-69	1.0073148325094457E-4
70-71	0.0028688648417490577
72-73	0.010283312596786547
74-75	0.012255433106414287
76-77	0.0050429380813739235
78-79	0.00458432481589871
80-81	3.343086281030014E-4
82-83	1.1502016073377341E-4
84-85	7.73543691836283E-5
86-87	0.0017794084512692218
88-89	0.004450825273597744
90-91	3.659502686313635E-4
92-93	0.0010765148170214026
94-95	0.0023778603509397255
96-97	0.0025778816763841
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	195.0
22-23	405.0
24-25	600.0
26-27	1697.0
28-29	4316.0
30-31	12966.0
32-33	16643.0
34-35	23297.0
36-37	36738.0
38-39	41559.0
40-41	42405.0
42-43	48972.0
44-45	58907.0
46-47	59831.0
48-49	55726.0
50-51	49101.0
52-53	46672.0
54-55	51045.0
56-57	53147.0
58-59	29769.0
60-61	54996.0
62-63	53611.0
64-65	58343.0
66-67	63267.0
68-69	64546.0
70-71	69173.0
72-73	57700.0
74-75	56970.0
76-77	59802.0
78-79	61343.0
80-81	60952.0
82-83	57408.0
84-85	56608.0
86-87	58781.0
88-89	61869.0
90-91	61514.0
92-93	61405.0
94-95	73114.0
96-97	264358.0
98-99	538526.0
100-101	5301565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.28636994654316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.12391868262645	75.96217951116026
2	6.722778066938042	11.33277118436892
3	1.6845372006917614	4.259505770586601
4	0.6293967059086857	2.1219825438942044
5	0.29613156624125364	1.2479927372529775
6	0.15508078389375568	0.7842717793721402
7	0.09912140758772546	0.5848208540692782
8	0.06199819363341315	0.41804821476826215
9	0.046891350753135747	0.3557071563184723
>10	0.17312699337366982	2.3601726187934786
>50	0.005735594045001909	0.33638817924102077
>100	0.0011719028247922556	0.15504140586831983
>500	8.36636420813621E-5	0.05187701795824704
>1k	2.7887840233836805E-5	0.02924102634785319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5543152793903668E-5	0.0	1.2771576396951834E-5	0.0
3	0.0	2.5543152793903668E-5	0.0	1.2771576396951834E-5	0.0
4	0.0	1.0217261117561467E-4	0.0	1.2771576396951834E-5	0.0
5	0.0	1.149441875725665E-4	0.0	1.2771576396951834E-5	0.0
6	5.1086305587807336E-5	1.149441875725665E-4	0.0	1.2771576396951834E-5	0.0
7	5.1086305587807336E-5	1.149441875725665E-4	0.0	1.2771576396951834E-5	0.0
8	5.1086305587807336E-5	1.2771576396951832E-4	0.0	2.5543152793903668E-5	2.5543152793903668E-5
9	5.1086305587807336E-5	2.682031043359885E-4	0.0	2.5543152793903668E-5	3.83147291908555E-5
10-11	6.385788198475916E-5	2.682031043359885E-4	0.0	5.7472093786283245E-5	3.83147291908555E-5
12-13	7.6629458381711E-5	2.682031043359885E-4	0.0	7.024367018323508E-5	3.83147291908555E-5
14-15	7.6629458381711E-5	4.150762329009346E-4	0.0	7.6629458381711E-5	3.83147291908555E-5
16-17	1.149441875725665E-4	4.470051738933142E-4	0.0	8.940103477866284E-5	3.83147291908555E-5
18-19	1.149441875725665E-4	4.789341148856937E-4	0.0	1.213299757710424E-4	4.470051738933141E-5
20-21	1.149441875725665E-4	5.36406208671977E-4	0.0	1.4048734036647016E-4	5.1086305587807336E-5
22-23	1.149441875725665E-4	5.874925142597843E-4	0.0	1.4048734036647016E-4	7.024367018323508E-5
24-25	1.149441875725665E-4	6.577361844430194E-4	0.0	1.53258916763422E-4	7.6629458381711E-5
26-27	1.149441875725665E-4	7.024367018323509E-4	0.0	2.1711679874818118E-4	7.6629458381711E-5
28-29	1.149441875725665E-4	7.088224900308268E-4	0.0	4.4700517389331413E-4	8.301524658018691E-5
30-31	1.149441875725665E-4	8.301524658018692E-4	0.0	0.001155827663924141	8.940103477866284E-5
32-33	1.149441875725665E-4	8.365382540003451E-4	0.0	0.002918305206703494	8.940103477866284E-5
34-35	1.2771576396951832E-4	9.706398061683393E-4	0.0	0.005127787923376161	8.940103477866284E-5
36-37	1.4048734036647016E-4	0.0011175129347332853	0.0	0.008671900373530295	8.940103477866284E-5
38-39	1.4048734036647016E-4	0.001149441875725665	0.0	0.018633729963152726	8.940103477866284E-5
40-41	1.4048734036647016E-4	0.0012643860632982316	0.0	0.029438483594973974	8.940103477866284E-5
42-43	1.4048734036647016E-4	0.001309086580687563	0.0	0.03527509400838097	8.940103477866284E-5
44-45	1.4048734036647016E-4	0.0014815028620464128	0.0	0.04277839514159017	8.940103477866284E-5
46-47	1.4048734036647016E-4	0.001609218626015931	0.0	0.05138005184493723	8.940103477866284E-5
48-49	1.4048734036647016E-4	0.001615604414214407	0.0	0.05996255118368886	8.940103477866284E-5
50-51	1.4687312856494607E-4	0.0017944064837717327	0.0	0.06808527377215022	8.940103477866284E-5
52-53	1.53258916763422E-4	0.0018007922719702086	0.0	0.09332829452072552	9.578682297713876E-5
54-55	1.53258916763422E-4	0.0018071780601686845	0.0	0.12396730629701297	1.0217261117561467E-4
56-57	1.53258916763422E-4	0.0018263354247641122	0.0	0.16136248198728795	1.1494418757256649E-4
58-59	1.53258916763422E-4	0.00184549278935954	0.0	0.34275079576499634	1.2771576396951832E-4
60-61	1.596447049618979E-4	0.0019093506713442991	0.0	0.5084875426682403	1.2771576396951832E-4
62-63	1.915736459542775E-4	0.0019348938241382028	0.0	0.5143177672934488	1.2771576396951832E-4
64-65	1.915736459542775E-4	0.0021903253520772392	0.0	0.5202757076826269	1.2771576396951832E-4
66-67	1.915736459542775E-4	0.002209482716672667	0.0	0.5258696581444917	1.2771576396951832E-4
68-69	1.915736459542775E-4	0.0022286400812680947	0.0	0.5314316796653642	1.2771576396951832E-4
70-71	1.9795943415275343E-4	0.0022669548104589502	6.385788198475917E-6	0.5363040360608013	1.4048734036647016E-4
72-73	2.1711679874818118E-4	0.002292497963252854	1.2771576396951834E-5	0.5401035800388946	1.53258916763422E-4
74-75	2.1711679874818118E-4	0.0023308126924437094	1.2771576396951834E-5	0.543155986797766	1.53258916763422E-4
76-77	2.1711679874818118E-4	0.002452142668214752	1.2771576396951834E-5	0.5461062209454619	1.53258916763422E-4
78-79	2.1711679874818118E-4	0.0025032289738025596	1.2771576396951834E-5	0.548187987898165	1.53258916763422E-4
80-81	2.1711679874818118E-4	0.0025032289738025596	1.2771576396951834E-5	0.5489670540583791	1.596447049618979E-4
82-83	2.235025869466571E-4	0.0025096147620010355	1.2771576396951834E-5	0.5494779171142572	1.6603049316037385E-4
84-85	2.29888375145133E-4	0.0025160005501995114	1.2771576396951834E-5	0.5497397344303947	1.6603049316037385E-4
86-87	2.3627416334360892E-4	0.0025670868557873187	1.2771576396951834E-5	0.5498482928297688	1.6603049316037385E-4
88	2.4265995154208484E-4	0.002694802619756837	1.2771576396951834E-5	0.5498546786179673	1.6603049316037385E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10015	0.0	51.777275	1
GTATCAA	18340	0.0	36.789433	1
ATCAACG	23120	0.0	29.964905	3
TCAACGC	23735	0.0	29.243378	4
CAACGCA	24005	0.0	28.86018	5
TATCAAC	24145	0.0	28.766325	2
AACGCAG	24195	0.0	28.65119	6
ACGCAGA	28100	0.0	24.684944	7
CGCAGAG	28990	0.0	23.912231	8
TGGTATC	4610	0.0	21.804226	2
TACATGG	25185	0.0	21.309052	2
GTACATG	24680	0.0	21.185717	1
ACATGGG	24995	0.0	20.899996	3
GCAGAGT	33475	0.0	20.734228	9
GTGGTAT	5065	0.0	20.61207	1
GAGTACT	19995	0.0	19.707613	12-13
ACTTCGC	1300	0.0	19.245848	6
CAGAGTA	32625	0.0	18.881102	10-11
CGAGACC	7915	0.0	18.84962	86-87
AGAGTAC	30480	0.0	18.709671	10-11
>>END_MODULE