FastQCFastQC Report
Fri 27 May 2016
SRR2138991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930146
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88600.044.300811
GTATCAA150950.033.506361
TCAACGC168500.030.015014
ATCAACG169200.029.9684873
CAACGCA171700.029.4535755
ATCTCGT96650.029.09797594
AACGCAG179950.028.2494356
TATCAAC184400.027.7140462
ACGCAGA209850.024.1825437
CGCAGAG216900.023.3965248
TATCTCG110400.023.14778194
GTACATG200150.020.751211
TACATGG203150.020.6392732
ACATGGG200850.020.1312623
GCAGAGT262150.019.45859
GAGTACT151200.018.63947112-13
CGCTATC151700.018.33224794
CATGGGG157150.017.963154
AGAGTAC235950.017.80506910-11
CAGAGTA258350.017.63764610-11
Produced by FastQC (version 0.11.5)