##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7930146
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.364657346787816	18.0	18.0	28.0	18.0	33.0
2	30.898152946994923	32.0	28.0	33.0	27.0	33.0
3	31.729854406211437	33.0	31.0	33.0	29.0	33.0
4	32.56235408528418	33.0	33.0	33.0	31.0	34.0
5	32.93722927673715	33.0	33.0	34.0	32.0	34.0
6	37.184895334839986	38.0	38.0	38.0	36.0	38.0
7	37.29130169860681	38.0	38.0	38.0	36.0	38.0
8	37.358506009851524	38.0	38.0	38.0	37.0	38.0
9	37.43710468886702	38.0	38.0	38.0	37.0	38.0
10-11	37.455246977798396	38.0	38.0	38.0	37.0	38.0
12-13	37.477947820885014	38.0	38.0	38.0	37.0	38.0
14-15	37.46589930374547	38.0	38.0	38.0	37.0	38.0
16-17	37.40627978349957	38.0	38.0	38.0	37.0	38.0
18-19	37.43412088503793	38.0	38.0	38.0	37.0	38.0
20-21	37.45825448152638	38.0	38.0	38.0	37.0	38.0
22-23	37.46526141072268	38.0	38.0	38.0	37.0	38.0
24-25	37.46537534333601	38.0	38.0	38.0	37.0	38.0
26-27	37.43393437970289	38.0	38.0	38.0	37.0	38.0
28-29	37.40731188690162	38.0	38.0	38.0	37.0	38.0
30-31	37.31269391616232	38.0	38.0	38.0	37.0	38.0
32-33	37.37592029257148	38.0	38.0	38.0	37.0	38.0
34-35	37.38361894823203	38.0	38.0	38.0	37.0	38.0
36-37	37.36286058827482	38.0	38.0	38.0	37.0	38.0
38-39	37.340327038514815	38.0	38.0	38.0	37.0	38.0
40-41	37.33614793123007	38.0	38.0	38.0	37.0	38.0
42-43	37.320049043164204	38.0	38.0	38.0	37.0	38.0
44-45	37.28925275496163	38.0	38.0	38.0	37.0	38.0
46-47	37.266928992393304	38.0	38.0	38.0	37.0	38.0
48-49	37.239438013673805	38.0	38.0	38.0	37.0	38.0
50-51	37.1969206790078	38.0	38.0	38.0	37.0	38.0
52-53	37.17573434962766	38.0	38.0	38.0	37.0	38.0
54-55	37.16321922198415	38.0	38.0	38.0	36.5	38.0
56-57	37.15537000136902	38.0	38.0	38.0	36.0	38.0
58-59	37.14512760212568	38.0	38.0	38.0	36.0	38.0
60-61	37.1385102399246	38.0	38.0	38.0	36.0	38.0
62-63	37.14291958548726	38.0	38.0	38.0	36.0	38.0
64-65	37.146255308237926	38.0	38.0	38.0	36.0	38.0
66-67	37.12428414377811	38.0	38.0	38.0	36.0	38.0
68-69	37.12197807489401	38.0	38.0	38.0	36.0	38.0
70-71	37.10284724144557	38.0	38.0	38.0	36.0	38.0
72-73	37.093044186253934	38.0	38.0	38.0	36.0	38.0
74-75	37.07796163706827	38.0	38.0	38.0	36.0	38.0
76-77	37.07683927010958	38.0	38.0	38.0	36.0	38.0
78-79	37.05665833385093	38.0	38.0	38.0	36.0	38.0
80-81	37.04956610972177	38.0	38.0	38.0	36.0	38.0
82-83	36.98652336541923	38.0	38.0	38.0	36.0	38.0
84-85	36.99068476988052	38.0	38.0	38.0	36.0	38.0
86-87	36.98160942322115	38.0	38.0	38.0	36.0	38.0
88-89	36.981859957201436	38.0	38.0	38.0	36.0	38.0
90-91	36.97230214713948	38.0	38.0	38.0	36.0	38.0
92-93	36.95576102098525	38.0	38.0	38.0	36.0	38.0
94-95	36.90899061180009	38.0	38.0	38.0	35.5	38.0
96-97	36.84134777956457	38.0	38.0	38.0	35.0	38.0
98-99	36.854940615710916	38.0	38.0	38.0	35.0	38.0
100	35.28272619555191	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	32.0
20	89.0
21	295.0
22	748.0
23	1694.0
24	3752.0
25	7104.0
26	12200.0
27	19461.0
28	29363.0
29	42482.0
30	58348.0
31	77537.0
32	100680.0
33	137303.0
34	203043.0
35	365563.0
36	1169015.0
37	5701430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20255112579264	18.38764380882773	17.696332450877954	23.713472614501676
2	14.15415001868307	20.970727671209243	42.34250764757387	22.532614662533817
3	18.447554433424052	25.6870050059608	30.37739784362104	25.488042716994112
4	12.40472449960895	18.12952701283169	38.91931522952754	30.54643325803182
5	13.314663325098369	37.41660318418302	35.97417102553099	13.294562465187617
6	29.823826698777044	37.99051114569644	18.829665935532585	13.355996219993932
7	26.27179877898843	32.38681860333971	23.40210382003055	17.939278797641304
8	23.741050921382783	37.44996624274005	21.382544533228014	17.426438302649156
9	25.111883242443827	17.07362869416462	21.564733645374826	36.24975441801673
10-11	24.079452769945473	26.883426732508465	29.13862589721215	19.898494600333915
12-13	24.73076469597971	24.30959333026067	29.33001855577672	21.6296234179829
14-15	21.886892120271177	25.74796226954712	26.743700557341565	25.621445052840137
16-17	21.676605954039182	28.89392578648615	27.47059764095138	21.958870618523292
18-19	21.682721856571117	28.086456415808737	29.098537403977176	21.13228432364297
20-21	22.296034699773852	27.26514566907612	28.964825766901104	21.473993864248918
22-23	22.123414153618466	27.240198060593112	28.83957726274278	21.796810523045647
24-25	22.01255724052209	27.355396920272163	28.838938581434505	21.79310725777125
26-27	21.94654717071559	27.42569549720893	28.852357085381758	21.775400246693724
28-29	21.897302754921945	27.413504725636557	28.906063974520247	21.783128544921254
30-31	21.979221286488094	27.209102524020413	28.954689684299723	21.856986505191774
32-33	21.722019190684524	27.511125991988855	28.877261376759083	21.889593440567534
34-35	21.987909882841155	27.369131485551744	28.9023451598763	21.740613471730796
36-37	21.92302967065423	27.402160020933238	28.886967056779376	21.787843251633156
38-39	21.796284298866784	27.4307759006862	28.932860926838888	21.840078873608128
40-41	22.04553662678562	27.28335011052455	28.908118623706812	21.76299463898302
42-43	21.89686723463765	27.39074041845037	28.752887311687548	21.95950503522443
44-45	21.930486342592786	27.442406610813467	28.7165351328139	21.910571913779847
46-47	21.948516107304364	27.37359366797001	28.61138294403982	22.066507280685805
48-49	21.867048149794577	27.674388317195174	28.447356404896563	22.011207128113686
50-51	21.799569145670485	27.66276162942259	28.419264439240138	22.118404785666787
52-53	21.84658548189566	27.51843906987031	28.388946590017788	22.246028858216242
54-55	21.99294456907131	27.27817047497478	28.584692004679766	22.144192951274142
56-57	21.97169702064516	27.10738852237309	28.56763573873457	22.353278718247182
58-59	21.677451200966164	27.138171334938654	28.744900682703605	22.439476781391576
60-61	21.92828430942348	27.40926004123434	28.82839439748593	21.83406125185625
62-63	21.493986292662502	27.7376311778633	28.718681930470737	22.049700599003465
64-65	21.28160568378469	27.97440094668422	28.506870237681532	22.23712313184956
66-67	21.40729393624921	28.420592444425147	28.039355409187305	22.13275821013834
68-69	21.408833700872467	28.39737627114542	27.945458530969276	22.24833149701284
70-71	21.459502884623387	27.88956290809998	27.952715208999912	22.69821899827672
72-73	21.732692197368205	27.31751535553886	27.956428494326786	22.993363952766156
74-75	21.775964970209472	27.430175127066793	28.11091693888079	22.682942963842947
76-77	22.129551870657902	27.3068833601667	27.54220011416456	23.02136465501084
78-79	22.20544876488936	27.342711942755038	27.18418770211536	23.26765159024024
80-81	22.137703646097016	27.6715288290026	27.290189196945256	22.900578327955124
82-83	22.05620912904678	27.541464359729435	27.368415596770756	23.033910914453028
84-85	22.181562252113686	27.881446921147525	27.25268743085313	22.684303395885667
86-87	22.584481258399588	27.775853065591523	27.297557139460093	22.3421085365488
88-89	22.330747576878096	27.86276301819478	27.577900807785916	22.22858859714121
90-91	22.334039413299603	27.581747008100415	27.400052991884476	22.684160586715507
92-93	22.392633873391304	27.15808541343265	27.685256903837413	22.764023809338628
94-95	21.745090734713255	27.667210872476083	28.100504627904954	22.487193764905708
96-97	21.83802684651277	27.516180253707002	28.321966063340465	22.32382683643976
98-99	23.067426400759732	29.343797467496547	28.605801528786955	18.982974602956762
100	22.03498166220055	30.3921973939546	22.349053775300305	25.22376716854454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1292.0
1	1258.5
2	1768.0
3	2502.0
4	2993.0
5	3793.5
6	4674.5
7	6483.0
8	8661.5
9	9660.0
10	9474.0
11	9174.5
12	9807.0
13	11493.0
14	14675.0
15	18298.5
16	20893.5
17	22003.0
18	21638.0
19	20438.5
20	19549.0
21	19737.5
22	22030.5
23	26909.5
24	34473.0
25	45378.0
26	59336.5
27	74940.5
28	90377.5
29	107374.5
30	125354.0
31	143946.5
32	163402.5
33	182540.5
34	200506.5
35	216362.0
36	234240.5
37	252482.5
38	266285.5
39	275800.5
40	285350.0
41	296937.5
42	306494.5
43	315072.0
44	329717.5
45	343171.0
46	340853.5
47	333617.5
48	330247.0
49	326036.0
50	317928.5
51	304900.0
52	290288.0
53	273663.0
54	255194.5
55	235675.0
56	214705.0
57	192427.5
58	170839.5
59	147144.0
60	119677.0
61	93899.0
62	72412.5
63	54729.0
64	40150.5
65	28687.5
66	20561.5
67	15217.5
68	11277.0
69	8198.0
70	5957.0
71	4256.0
72	2985.5
73	2025.5
74	1300.5
75	797.0
76	483.0
77	285.0
78	175.5
79	115.0
80	70.0
81	42.0
82	29.5
83	21.5
84	16.0
85	11.0
86	7.0
87	4.5
88	4.5
89	5.0
90	3.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006393324914825024
3	0.0
4	0.0031273068616895577
5	0.0017275848389172153
6	0.0
7	0.0
8	0.0
9	5.0440433253057384E-5
10-11	3.27862816144873E-4
12-13	1.2610108313264346E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.548662113527014E-4
24-25	0.0012422018728116438
26-27	0.0
28-29	0.001791331682911333
30-31	0.002619473557771511
32-33	5.184115768132962E-4
34-35	1.2670819274267423E-5
36-37	8.770833191654717E-4
38-39	0.0021431199757470255
40-41	0.0013511219530999315
42-43	0.0
44-45	0.0
46-47	0.0011538867730676308
48-49	4.7262863178999955E-4
50-51	0.0014735606220196741
52-53	0.0021321556417997064
54-55	0.0016894903562896645
56-57	0.001584346707561815
58-59	0.0016847096416198097
60-61	8.002460489851946E-4
62-63	6.984239593264748E-4
64-65	0.0029691217425809324
66-67	0.0021677767887958934
68-69	1.031382139390471E-4
70-71	0.0025182585888053484
72-73	0.009063266573573238
74-75	0.011231123101015447
76-77	0.004390204507395217
78-79	0.0041616980416842855
80-81	3.1845753068464967E-4
82-83	2.920037332677298E-5
84-85	6.62440492602527E-5
86-87	0.0017292423565817968
88-89	0.004162552465193088
90-91	4.7597274474291877E-4
92-93	0.0010903827403544782
94-95	0.0025481331575865625
96-97	0.0027563357070094787
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	186.0
22-23	368.0
24-25	475.0
26-27	1373.0
28-29	3423.0
30-31	12085.0
32-33	15264.0
34-35	22029.0
36-37	34997.0
38-39	21664.0
40-41	39615.0
42-43	43204.0
44-45	52032.0
46-47	53608.0
48-49	51050.0
50-51	26536.0
52-53	4510.0
54-55	4120.0
56-57	4129.0
58-59	28384.0
60-61	52898.0
62-63	51888.0
64-65	55969.0
66-67	61900.0
68-69	63871.0
70-71	71416.0
72-73	56480.0
74-75	55331.0
76-77	58007.0
78-79	59881.0
80-81	59846.0
82-83	56831.0
84-85	55382.0
86-87	57830.0
88-89	60152.0
90-91	61062.0
92-93	61004.0
94-95	72721.0
96-97	266409.0
98-99	608808.0
100-101	5563372.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98069300698396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54540228445384	76.09630341722053
2	7.355247393214636	12.501080414263841
3	1.745228335213307	4.449321401455552
4	0.6402092160141512	2.176216913853618
5	0.29306072631537355	1.245225180770525
6	0.14236303448873752	0.7258865597658043
7	0.08057427361080771	0.47930803269865446
8	0.05242839074479464	0.35643207829868734
9	0.030488950266457975	0.23318749104291597
>10	0.11240304176298986	1.46875504656924
>50	0.00174373170198844	0.09714744783070817
>100	7.561142677892178E-4	0.10919431728241885
>500	7.088107595566835E-5	0.040437864106204556
>1k	2.362686917743868E-5	0.02150383484140258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5220216626528692E-5	0.0	1.2610108313264346E-5	0.0
3	0.0	2.5220216626528692E-5	0.0	1.2610108313264346E-5	0.0
4	0.0	5.0440433253057384E-5	0.0	1.2610108313264346E-5	0.0
5	0.0	5.0440433253057384E-5	0.0	1.2610108313264346E-5	0.0
6	8.827075819285042E-5	5.0440433253057384E-5	0.0	1.2610108313264346E-5	0.0
7	8.827075819285042E-5	5.0440433253057384E-5	0.0	3.783032493979304E-5	0.0
8	1.0088086650611477E-4	5.0440433253057384E-5	0.0	3.783032493979304E-5	0.0
9	1.0088086650611477E-4	7.566064987958608E-5	0.0	3.783032493979304E-5	1.2610108313264346E-5
10-11	1.1349097481937911E-4	7.566064987958608E-5	0.0	5.0440433253057384E-5	1.2610108313264346E-5
12-13	1.1349097481937911E-4	7.566064987958608E-5	0.0	6.305054156632172E-5	1.2610108313264346E-5
14-15	1.1349097481937911E-4	8.827075819285043E-5	0.0	9.457581234948259E-5	1.2610108313264346E-5
16-17	1.2610108313264345E-4	1.0088086650611477E-4	0.0	1.0088086650611477E-4	1.2610108313264346E-5
18-19	1.2610108313264345E-4	1.2610108313264345E-4	0.0	1.1349097481937911E-4	1.2610108313264346E-5
20-21	1.2610108313264345E-4	1.639314080724365E-4	0.0	1.2610108313264345E-4	1.2610108313264346E-5
22-23	1.387111914459078E-4	1.891516246989652E-4	0.0	1.5132129975917215E-4	1.891516246989652E-5
24-25	1.387111914459078E-4	2.080667871688617E-4	0.0	1.8915162469896517E-4	2.5220216626528692E-5
26-27	1.5132129975917215E-4	2.2698194963875821E-4	0.0	2.711173287351834E-4	3.783032493979304E-5
28-29	1.5762635391580434E-4	2.332870037953904E-4	0.0	4.854891700606773E-4	3.783032493979304E-5
30-31	1.7654151638570083E-4	2.3959205795202258E-4	0.0	0.0011538249106636876	3.783032493979304E-5
32-33	1.7654151638570083E-4	2.3959205795202258E-4	0.0	0.0029381552369905925	3.783032493979304E-5
34-35	1.8915162469896517E-4	2.7111732873518347E-4	0.0	0.0053151606540409214	3.783032493979304E-5
36-37	2.0176173301222954E-4	3.026425995183443E-4	0.0	0.011368012644407809	3.783032493979304E-5
38-39	2.0176173301222954E-4	3.27862816144873E-4	0.0	0.13879315714994403	3.783032493979304E-5
40-41	2.0176173301222954E-4	3.27862816144873E-4	0.0	0.27015265544921874	3.783032493979304E-5
42-43	2.1437184132549388E-4	3.3416787030150514E-4	0.0	0.2836013359653151	3.783032493979304E-5
44-45	2.3959205795202255E-4	3.467779786147695E-4	0.0	0.30155182514924694	3.783032493979304E-5
46-47	2.522021662652869E-4	3.5308303277140166E-4	0.0	0.32224501289131374	3.783032493979304E-5
48-49	2.522021662652869E-4	3.5938808692803387E-4	0.0	0.34290667536259734	3.783032493979304E-5
50-51	2.6481227457855126E-4	3.972184118678269E-4	0.0	0.3649617548024967	3.783032493979304E-5
52-53	2.6481227457855126E-4	4.0352346602445907E-4	0.0	0.7872049266179967	3.783032493979304E-5
54-55	2.6481227457855126E-4	4.0982852018109123E-4	0.0	1.352213439702119	3.783032493979304E-5
56-57	2.6481227457855126E-4	4.1613357433772344E-4	0.0	1.9688288210582758	3.783032493979304E-5
58-59	2.6481227457855126E-4	4.1613357433772344E-4	0.0	2.60652830351421	3.783032493979304E-5
60-61	2.6481227457855126E-4	4.3504873680761996E-4	0.0	2.774980435416952	3.783032493979304E-5
62-63	3.026425995183443E-4	4.5396389927751643E-4	0.0	2.7804973578040055	3.783032493979304E-5
64-65	3.026425995183443E-4	4.7918411590404516E-4	0.0	2.785900789216239	3.783032493979304E-5
66-67	3.026425995183443E-4	4.917942242173095E-4	0.0	2.7907872061876287	3.783032493979304E-5
68-69	3.026425995183443E-4	5.170144408438381E-4	0.0	2.795225964313898	3.783032493979304E-5
70-71	3.026425995183443E-4	5.296245491571025E-4	0.0	2.799015301862034	3.783032493979304E-5
72-73	3.4047292445813735E-4	5.296245491571025E-4	0.0	2.8020732531280004	7.566064987958608E-5
74-75	3.467779786147695E-4	5.422346574703669E-4	0.0	2.8045952747906533	7.566064987958608E-5
76-77	3.5308303277140166E-4	5.611498199402634E-4	0.0	2.806915534720294	7.566064987958608E-5
78-79	3.5308303277140166E-4	5.674548740968956E-4	0.0	2.80881335602144	7.566064987958608E-5
80-81	3.5308303277140166E-4	5.674548740968956E-4	0.0	2.809342980570597	8.196570403621825E-5
82-83	3.5308303277140166E-4	5.674548740968956E-4	0.0	2.8098726051197547	8.827075819285042E-5
84-85	3.5938808692803387E-4	5.674548740968956E-4	0.0	2.8101311123401764	8.827075819285042E-5
86-87	3.7830324939793034E-4	5.926750907234243E-4	0.0	2.810225688152526	8.827075819285042E-5
88	3.909133577111947E-4	6.55725632289746E-4	0.0	2.810238298260839	8.827075819285042E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8860	0.0	44.30081	1
GTATCAA	15095	0.0	33.50636	1
TCAACGC	16850	0.0	30.01501	4
ATCAACG	16920	0.0	29.968487	3
CAACGCA	17170	0.0	29.453575	5
ATCTCGT	9665	0.0	29.097975	94
AACGCAG	17995	0.0	28.249435	6
TATCAAC	18440	0.0	27.714046	2
ACGCAGA	20985	0.0	24.182543	7
CGCAGAG	21690	0.0	23.396524	8
TATCTCG	11040	0.0	23.147781	94
GTACATG	20015	0.0	20.75121	1
TACATGG	20315	0.0	20.639273	2
ACATGGG	20085	0.0	20.131262	3
GCAGAGT	26215	0.0	19.4585	9
GAGTACT	15120	0.0	18.639471	12-13
CGCTATC	15170	0.0	18.332247	94
CATGGGG	15715	0.0	17.96315	4
AGAGTAC	23595	0.0	17.805069	10-11
CAGAGTA	25835	0.0	17.637646	10-11
>>END_MODULE