FastQCFastQC Report
Fri 27 May 2016
SRR2138990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789888
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87300.050.5744131
GTATCAA156200.035.5093151
ATCAACG186250.029.7327043
TCAACGC187800.029.5814424
CAACGCA192050.028.9498275
TATCAAC198500.028.0759322
AACGCAG199300.028.0056086
ACGCAGA230300.024.1015327
CGCAGAG238800.023.2436478
GCAGAGT286350.019.3376129
GAGTACT149900.018.69117412-13
AGAGTAC261850.018.2187710-11
CAGAGTA282350.017.70988510-11
GTACATG228500.017.620241
TACATGG227350.017.4566542
ACATGGG230800.016.6978423
GTACTTT178850.015.59175314-15
AGTACTT173850.015.25195512-13
CATGGGG155750.014.783914
TATACAG68750.013.8211885
Produced by FastQC (version 0.11.5)