##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7789888
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.823629556676554	25.0	18.0	32.0	18.0	33.0
2	26.84320788694266	28.0	25.0	32.0	18.0	33.0
3	29.045937759310533	31.0	27.0	33.0	18.0	33.0
4	29.694972122834116	31.0	29.0	33.0	25.0	33.0
5	31.13926015367615	32.0	32.0	33.0	28.0	33.0
6	36.09763015848238	37.0	36.0	38.0	33.0	38.0
7	36.209633565976816	38.0	37.0	38.0	33.0	38.0
8	36.860002865253	38.0	38.0	38.0	35.0	38.0
9	37.00795531334982	38.0	38.0	38.0	36.0	38.0
10-11	37.07438714138124	38.0	38.0	38.0	36.0	38.0
12-13	37.12054114256841	38.0	38.0	38.0	36.0	38.0
14-15	37.14174632549274	38.0	38.0	38.0	36.0	38.0
16-17	37.11927251072159	38.0	38.0	38.0	36.0	38.0
18-19	37.15519696817207	38.0	38.0	38.0	36.0	38.0
20-21	37.121730970069066	38.0	38.0	38.0	36.0	38.0
22-23	37.162126065572956	38.0	38.0	38.0	36.0	38.0
24-25	37.16811642302762	38.0	38.0	38.0	36.0	38.0
26-27	37.09820539915117	38.0	38.0	38.0	36.0	38.0
28-29	37.08322425319558	38.0	38.0	38.0	36.0	38.0
30-31	36.954412045477596	38.0	38.0	38.0	36.0	38.0
32-33	37.05117363366482	38.0	38.0	38.0	36.0	38.0
34-35	37.07028489778696	38.0	38.0	38.0	36.0	38.0
36-37	36.99471464136606	38.0	38.0	38.0	36.0	38.0
38-39	37.03177524992876	38.0	38.0	38.0	36.0	38.0
40-41	36.948225996104924	38.0	38.0	38.0	36.0	38.0
42-43	36.930521991813194	38.0	38.0	38.0	35.5	38.0
44-45	36.927479712993325	38.0	38.0	38.0	36.0	38.0
46-47	36.888088425158664	38.0	38.0	38.0	35.0	38.0
48-49	36.85500213726401	38.0	38.0	38.0	35.0	38.0
50-51	36.87228800003016	38.0	38.0	38.0	35.0	38.0
52-53	36.78338088749864	38.0	38.0	38.0	35.0	38.0
54-55	36.76695845377678	38.0	38.0	38.0	35.0	38.0
56-57	36.77163888471556	38.0	38.0	38.0	35.0	38.0
58-59	36.79217788597499	38.0	38.0	38.0	35.0	38.0
60-61	36.787298040586734	38.0	38.0	38.0	35.0	38.0
62-63	36.79286667259596	38.0	38.0	38.0	35.0	38.0
64-65	36.74638683149688	38.0	38.0	38.0	35.0	38.0
66-67	36.75026549919611	38.0	38.0	38.0	35.0	38.0
68-69	36.763255752873064	38.0	38.0	38.0	35.0	38.0
70-71	36.679338877168504	38.0	38.0	38.0	34.5	38.0
72-73	36.63134536761455	38.0	38.0	38.0	34.0	38.0
74-75	36.682177200008326	38.0	38.0	38.0	34.0	38.0
76-77	36.65548319312147	38.0	38.0	38.0	34.0	38.0
78-79	36.67466671039864	38.0	38.0	38.0	34.0	38.0
80-81	36.698520134580725	38.0	38.0	38.0	34.0	38.0
82-83	36.57030639922161	38.0	38.0	38.0	34.0	38.0
84-85	36.58521626291852	38.0	38.0	38.0	34.0	38.0
86-87	36.582705887456385	38.0	38.0	38.0	34.0	38.0
88-89	36.52900098572955	38.0	38.0	38.0	34.0	38.0
90-91	36.5247808572764	38.0	38.0	38.0	34.0	38.0
92-93	36.572376480946346	38.0	38.0	38.0	34.0	38.0
94-95	36.46958653667882	38.0	38.0	38.0	34.0	38.0
96-97	36.425956021226156	38.0	38.0	38.0	34.0	38.0
98-99	36.49404673528389	38.0	38.0	38.0	34.0	38.0
100	34.87083177195132	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	23.0
19	41.0
20	108.0
21	285.0
22	896.0
23	2528.0
24	5865.0
25	11719.0
26	20167.0
27	31821.0
28	47832.0
29	67413.0
30	91413.0
31	120683.0
32	160729.0
33	221423.0
34	334592.0
35	618254.0
36	1772904.0
37	4281188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.93058231389206	24.03954973421954	14.941331634036331	24.088536317852068
2	13.445918606275212	21.779286172021987	40.92342534321418	23.851369878488626
3	16.118960888782997	27.120056154851007	32.60588598963169	24.15509696673431
4	12.009749049023554	19.440947032871332	40.61465325303779	27.93465066506733
5	12.715928992454185	37.66309492723649	36.499731304340905	13.121244775968416
6	27.532864083907764	39.980618460832616	19.95708025032969	12.52943720492993
7	24.395369997617426	33.446039275532584	25.41155919058143	16.74703153626856
8	22.97909803067772	37.82368886433284	22.428024639121897	16.769188465867547
9	24.80832197030868	17.01077525396392	22.624284874002008	35.55661790172539
10-11	22.89052166090193	27.74302403320818	30.160760976281047	19.20569332960885
12-13	23.846812688449436	25.36933778765497	30.20868849462277	20.57516102927282
14-15	21.256473777286658	26.60072262913151	27.71347932088369	24.429324272698143
16-17	20.75029191690561	29.55264568630512	28.489299204301783	21.20776319248749
18-19	20.81033770960507	28.975063569591757	29.877650359029552	20.336948361773622
20-21	21.760498149532793	28.062375754826235	29.63004327652243	20.54708281911854
22-23	21.469018085992666	28.064320643468328	29.398803125192362	21.067858145346648
24-25	21.255811618692594	28.142799626865	29.545990840064718	21.05539791437769
26-27	21.182011151630007	28.31274380749427	29.474847524522385	21.03039751635334
28-29	21.114767166997286	28.339927760079636	29.496702615409742	21.048602457513333
30-31	21.35490950923053	28.17298665946419	29.49986559584397	20.972238235461308
32-33	21.276616124489774	28.19886401440505	29.394577084667922	21.12994277643725
34-35	21.362721640928054	28.258523245073413	29.417571817375805	20.961183296622725
36-37	21.31786419359872	28.248113155237707	29.356532099894135	21.077490551269438
38-39	21.19369229575607	28.320428272170066	29.420138619494658	21.0657408125792
40-41	21.321704511254786	28.162133272449903	29.408656690086033	21.10750552620928
42-43	21.282471696247928	28.231771180946748	29.190646467153776	21.29511065565155
44-45	21.319529399218208	28.284448937948888	29.138048511420283	21.25797315141262
46-47	21.35197763828705	28.274151637673423	29.00078647411623	21.373084249923295
48-49	21.388001870960117	28.375306626457874	28.94801921304704	21.288672289534972
50-51	21.359628783533697	28.402691058102608	28.877547386658865	21.360132771704833
52-53	21.46448454981353	28.396909962706445	28.826924613745337	21.311680873734684
54-55	21.492261662812577	28.35636356535304	28.772388385599264	21.37898638623512
56-57	21.46663042566959	28.39344179477313	28.70301076158762	21.43691701796966
58-59	21.38086378216283	28.415076574941377	28.880500396601665	21.323559246294124
60-61	21.477486621348184	28.474017971396258	28.77172249497401	21.276772912281537
62-63	21.41108398725981	28.536520936949483	28.799915349205673	21.25247972658503
64-65	21.474953191453164	28.41055503062055	28.749276451750944	21.365215326175342
66-67	21.459859616619102	28.489698797787593	28.65267719310814	21.39776439248516
68-69	21.455867970430827	28.446362223289217	28.643812420089816	21.453957386190144
70-71	21.431581077005614	28.346770783146503	28.70996634160698	21.511681798240904
72-73	21.47218614484562	28.37012028189923	28.65231397205705	21.50537960119809
74-75	21.477397260322533	28.37912732433682	28.57278173565372	21.570693679686926
76-77	21.5324064124711	28.315585427515323	28.51528193222338	21.636726227790195
78-79	21.507072771187712	28.31262074912927	28.567832392427295	21.612474087255727
80-81	21.487820617314856	28.367276132462404	28.54006363591399	21.604839614308744
82-83	21.649639480076193	28.299318358047486	28.559971913678332	21.491070248197985
84-85	21.614979263894718	28.36778719241248	28.501121172463016	21.516112371229788
86-87	21.55596317831839	28.38281690395277	28.566328586229318	21.494891331499524
88-89	21.625241423924557	28.32946395960978	28.50619541263765	21.539099203828012
90-91	21.68537086316799	28.365843052991973	28.4821907773116	21.46659530652844
92-93	21.675127762586328	28.355041381167762	28.505989548294576	21.463841307951338
94-95	21.537921561287384	28.504467766190427	28.47688981348083	21.480720859041355
96-97	21.617116724288838	28.591611409772423	28.55298523127211	21.238286634666633
98-99	22.63052281224356	30.442331850387433	28.864131581678944	18.063013755690065
100	21.46901879783323	31.406468790216802	22.908090832982815	24.216421578967157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	906.0
1	1041.5
2	1750.0
3	2490.5
4	3154.0
5	4215.0
6	5055.5
7	5945.0
8	6853.0
9	7509.5
10	7818.5
11	8095.0
12	9172.5
13	11328.0
14	15231.5
15	19713.0
16	22855.0
17	24323.5
18	24244.5
19	23030.5
20	22036.0
21	22486.0
22	25619.5
23	31905.5
24	41283.0
25	54628.0
26	71335.5
27	89800.5
28	109984.5
29	131624.5
30	152317.5
31	172522.5
32	194079.5
33	214448.0
34	233839.5
35	252277.5
36	269451.5
37	284791.0
38	295184.0
39	303551.0
40	311103.0
41	316333.0
42	318994.5
43	320919.0
44	321267.5
45	320709.0
46	317719.5
47	311560.0
48	303734.0
49	291913.0
50	279024.5
51	265645.5
52	247017.0
53	224246.0
54	203250.0
55	183129.0
56	162429.0
57	141006.0
58	119980.5
59	100669.0
60	81503.0
61	64743.5
62	50432.5
63	37886.5
64	28299.5
65	20309.5
66	14392.5
67	10325.5
68	7013.0
69	4659.5
70	3179.5
71	2147.5
72	1424.5
73	915.5
74	588.0
75	384.5
76	250.5
77	146.5
78	83.0
79	52.5
80	39.0
81	28.5
82	19.5
83	13.0
84	7.5
85	5.0
86	6.5
87	7.0
88	7.0
89	5.0
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0052118849462277255
6	1.2837155039969765E-5
7	0.0
8	0.0
9	2.567431007993953E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.419195139488147E-6
26-27	0.0
28-29	3.2103196624438764E-5
30-31	0.0
32-33	3.214264132797965E-5
34-35	0.0
36-37	4.900034145501098E-4
38-39	1.9396380751730012E-5
40-41	2.3350125532869322E-4
42-43	7.809855530038298E-5
44-45	7.645462770713094E-4
46-47	0.0
48-49	4.6199783969810154E-5
50-51	0.0
52-53	1.265315394180042E-4
54-55	0.002220247776976913
56-57	0.0011554707374348604
58-59	1.687807467886425E-4
60-61	4.6147109928659286E-4
62-63	1.3644532369811037E-4
64-65	7.751630088919429E-4
66-67	0.0014700553858895387
68-69	4.029579615496124E-4
70-71	6.575911164197214E-4
72-73	2.1837752543041504E-4
74-75	1.8433790044661527E-4
76-77	2.1412108575949176E-5
78-79	0.0016963983235271688
80-81	0.0
82-83	9.9223343867545E-4
84-85	1.7637710836785914E-4
86-87	0.0
88-89	7.461425363548624E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.950925717045828E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	57.0
20-21	214.0
22-23	368.0
24-25	513.0
26-27	969.0
28-29	1998.0
30-31	6138.0
32-33	8118.0
34-35	11940.0
36-37	20402.0
38-39	24150.0
40-41	25564.0
42-43	29150.0
44-45	36686.0
46-47	38577.0
48-49	36607.0
50-51	32771.0
52-53	30901.0
54-55	33228.0
56-57	35921.0
58-59	38056.0
60-61	38833.0
62-63	39668.0
64-65	42963.0
66-67	47135.0
68-69	48800.0
70-71	50782.0
72-73	45949.0
74-75	46095.0
76-77	48668.0
78-79	51275.0
80-81	51585.0
82-83	49965.0
84-85	49615.0
86-87	51669.0
88-89	55111.0
90-91	56397.0
92-93	58203.0
94-95	70442.0
96-97	244893.0
98-99	564125.0
100-101	5665387.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.22525700135662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0152231524068	77.61585750350744
2	7.1960761811597145	12.409670362436746
3	1.6427841248277428	4.249484500830626
4	0.5610540982536549	1.9350813525434312
5	0.2351011167475317	1.0135827106430932
6	0.12098300141018443	0.6259074233633186
7	0.06822725809723432	0.4118039004752343
8	0.042852389190467355	0.2955966616856163
9	0.03029347231051547	0.23508561918939094
>10	0.08622850630146889	1.1083883190884054
>50	8.671062812285714E-4	0.050037873022014207
>100	3.0959301336769415E-4	0.049503773214730866
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.567431007993953E-5	0.0	2.567431007993953E-5	0.0
5	0.0	2.567431007993953E-5	0.0	2.567431007993953E-5	0.0
6	5.134862015987906E-5	2.567431007993953E-5	0.0	2.567431007993953E-5	1.2837155039969765E-5
7	5.134862015987906E-5	3.85114651199093E-5	0.0	6.418577519984883E-5	1.2837155039969765E-5
8	5.134862015987906E-5	5.134862015987906E-5	0.0	6.418577519984883E-5	2.567431007993953E-5
9	5.134862015987906E-5	1.4120870543966744E-4	0.0	7.70229302398186E-5	3.85114651199093E-5
10-11	5.134862015987906E-5	1.4120870543966744E-4	0.0	8.344150775980348E-5	3.85114651199093E-5
12-13	5.134862015987906E-5	1.4120870543966744E-4	0.0	8.986008527978835E-5	3.85114651199093E-5
14-15	6.418577519984883E-5	1.7330159303959183E-4	0.0	8.986008527978835E-5	5.134862015987906E-5
16-17	1.0269724031975812E-4	1.797201705595767E-4	0.0	1.0269724031975812E-4	5.134862015987906E-5
18-19	1.1553439535972789E-4	1.9897590311953136E-4	0.0	1.4120870543966744E-4	6.418577519984883E-5
20-21	1.1553439535972789E-4	2.18231635679486E-4	0.0	1.6688301551960697E-4	6.418577519984883E-5
22-23	1.2195297287971279E-4	2.18231635679486E-4	0.0	1.9897590311953136E-4	7.060435271983372E-5
24-25	1.2837155039969767E-4	2.631616783193802E-4	0.0	2.5032452327941043E-4	7.70229302398186E-5
26-27	1.2837155039969767E-4	2.8241741087933487E-4	0.0	2.8883598839931973E-4	7.70229302398186E-5
28-29	1.2837155039969767E-4	2.8883598839931973E-4	0.0	4.5571900391892664E-4	7.70229302398186E-5
30-31	1.2837155039969767E-4	3.3376603103921394E-4	0.0	9.82042360557687E-4	7.70229302398186E-5
32-33	1.2837155039969767E-4	3.3376603103921394E-4	0.0	0.002009014763755268	7.70229302398186E-5
34-35	1.4120870543966744E-4	4.1078896127903254E-4	0.0	0.003305567422792215	7.70229302398186E-5
36-37	1.4120870543966744E-4	4.878118915188511E-4	0.0	0.006155415841665503	7.70229302398186E-5
38-39	1.4120870543966744E-4	5.006490465588209E-4	0.0	0.015340400272763871	7.70229302398186E-5
40-41	1.4120870543966744E-4	5.776719767986395E-4	0.0	0.025963146068338855	7.70229302398186E-5
42-43	1.4120870543966744E-4	6.03346286878579E-4	0.0	0.03204795755728452	7.70229302398186E-5
44-45	1.540458604796372E-4	6.932063721583673E-4	0.0	0.03955127467814685	7.70229302398186E-5
46-47	1.6688301551960697E-4	7.573921473582162E-4	0.0	0.04844100454332591	8.986008527978835E-5
48-49	1.7330159303959183E-4	7.573921473582162E-4	0.0	0.057459105958904674	8.986008527978835E-5
50-51	2.0539448063951624E-4	8.087407675180952E-4	0.0	0.06667618327760297	8.986008527978835E-5
52-53	2.18231635679486E-4	8.21577922558065E-4	0.0	0.09268425938858171	9.627866279977324E-5
54-55	2.18231635679486E-4	8.344150775980348E-4	0.0	0.12414170781402763	1.09115817839743E-4
56-57	2.18231635679486E-4	8.600893876779743E-4	0.0	0.16638878505056812	1.1553439535972789E-4
58-59	2.18231635679486E-4	8.600893876779743E-4	0.0	0.19968194664672972	1.1553439535972789E-4
60-61	2.246502131994709E-4	8.857636977579139E-4	0.0	0.21869377326092493	1.1553439535972789E-4
62-63	2.5674310079939534E-4	9.371123179177929E-4	0.0	0.23283389953745165	1.1553439535972789E-4
64-65	2.5674310079939534E-4	0.0010847396008774454	0.0	0.23726913660376117	1.1553439535972789E-4
66-67	2.631616783193802E-4	0.0010911581783974301	0.0	0.2410881902281522	1.2195297287971279E-4
68-69	2.695802558393651E-4	0.0011232510659973545	0.0	0.24529235850374229	1.2837155039969767E-4
70-71	2.7599883335935E-4	0.0011617625311172637	0.0	0.24891885480253373	1.2837155039969767E-4
72-73	2.8241741087933487E-4	0.0011681811086372486	0.0	0.2521281435625262	1.2837155039969767E-4
74-75	2.8241741087933487E-4	0.001200273996237173	0.0	0.2545736215976404	1.2837155039969767E-4
76-77	2.8241741087933487E-4	0.001315808391596901	0.0	0.25689714665987495	1.2837155039969767E-4
78-79	2.8241741087933487E-4	0.0013607384342367952	0.0	0.2583798380669915	1.2837155039969767E-4
80-81	2.8241741087933487E-4	0.0013607384342367952	0.0	0.2588291384933904	1.2837155039969767E-4
82-83	2.8241741087933487E-4	0.0013607384342367952	0.0	0.25936188042754915	1.4762728295965232E-4
84-85	2.8883598839931973E-4	0.001373575589276765	0.0	0.25968280930354837	1.540458604796372E-4
86-87	2.9525456591930464E-4	0.0014120870543966743	0.0	0.2597662508113082	1.540458604796372E-4
88	2.9525456591930464E-4	0.0014634356745565533	0.0	0.2597726693888282	1.540458604796372E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8730	0.0	50.574413	1
GTATCAA	15620	0.0	35.509315	1
ATCAACG	18625	0.0	29.732704	3
TCAACGC	18780	0.0	29.581442	4
CAACGCA	19205	0.0	28.949827	5
TATCAAC	19850	0.0	28.075932	2
AACGCAG	19930	0.0	28.005608	6
ACGCAGA	23030	0.0	24.101532	7
CGCAGAG	23880	0.0	23.243647	8
GCAGAGT	28635	0.0	19.337612	9
GAGTACT	14990	0.0	18.691174	12-13
AGAGTAC	26185	0.0	18.21877	10-11
CAGAGTA	28235	0.0	17.709885	10-11
GTACATG	22850	0.0	17.62024	1
TACATGG	22735	0.0	17.456654	2
ACATGGG	23080	0.0	16.697842	3
GTACTTT	17885	0.0	15.591753	14-15
AGTACTT	17385	0.0	15.251955	12-13
CATGGGG	15575	0.0	14.78391	4
TATACAG	6875	0.0	13.821188	5
>>END_MODULE