FastQCFastQC Report
Fri 27 May 2016
SRR2138989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9043128
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89100.049.440831
GTATCAA157800.034.0672381
TCAACGC185800.029.4060824
ATCAACG188350.028.8680553
CAACGCA193250.028.2497185
AACGCAG200450.027.3432066
TATCAAC204050.026.797562
ACGCAGA233200.023.4090337
CGCAGAG239000.022.8225738
GCAGAGT296750.018.4699089
GAGTACT156600.018.2570312-13
AGAGTAC264600.018.01693310-11
GTACATG230000.017.0333271
CAGAGTA292900.017.0108510-11
TACATGG233350.016.939372
ACATGGG230000.016.804183
GTACTTT176500.015.97477714-15
CATGGGG166150.014.6972794
AGTACTT193050.013.79754712-13
ATGGGGG95650.012.5354585
Produced by FastQC (version 0.11.5)