##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9043128
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.267370648740126	25.0	18.0	32.0	18.0	33.0
2	26.50458889888543	28.0	18.0	31.0	18.0	33.0
3	28.78272816662553	30.0	27.0	32.0	18.0	33.0
4	29.53127125923685	31.0	29.0	33.0	25.0	33.0
5	31.065920331991318	32.0	32.0	33.0	27.0	33.0
6	36.066951944061834	37.0	36.0	38.0	33.0	38.0
7	36.17956242574472	38.0	37.0	38.0	33.0	38.0
8	36.85443167452678	38.0	38.0	38.0	35.0	38.0
9	37.01326288868188	38.0	38.0	38.0	36.0	38.0
10-11	37.08707379791594	38.0	38.0	38.0	36.0	38.0
12-13	37.13234010399941	38.0	38.0	38.0	36.0	38.0
14-15	37.15702105510394	38.0	38.0	38.0	36.0	38.0
16-17	37.13296826053994	38.0	38.0	38.0	36.0	38.0
18-19	37.170404919625156	38.0	38.0	38.0	36.0	38.0
20-21	37.135643038282005	38.0	38.0	38.0	36.0	38.0
22-23	37.178263177752754	38.0	38.0	38.0	36.0	38.0
24-25	37.18559484582379	38.0	38.0	38.0	36.0	38.0
26-27	37.117738334512225	38.0	38.0	38.0	36.0	38.0
28-29	37.10686200962563	38.0	38.0	38.0	36.0	38.0
30-31	36.979413790861415	38.0	38.0	38.0	36.0	38.0
32-33	37.07751517694155	38.0	38.0	38.0	36.0	38.0
34-35	37.09425601737054	38.0	38.0	38.0	36.0	38.0
36-37	37.02114356805157	38.0	38.0	38.0	36.0	38.0
38-39	37.06233627108208	38.0	38.0	38.0	36.0	38.0
40-41	36.97568485817084	38.0	38.0	38.0	36.0	38.0
42-43	36.95797700291082	38.0	38.0	38.0	36.0	38.0
44-45	36.958501666049045	38.0	38.0	38.0	36.0	38.0
46-47	36.92121632419369	38.0	38.0	38.0	36.0	38.0
48-49	36.88757316800875	38.0	38.0	38.0	35.0	38.0
50-51	36.90458967564872	38.0	38.0	38.0	35.5	38.0
52-53	36.81338666873071	38.0	38.0	38.0	35.0	38.0
54-55	36.80077390331493	38.0	38.0	38.0	35.0	38.0
56-57	36.80552644684812	38.0	38.0	38.0	35.0	38.0
58-59	36.82720140713836	38.0	38.0	38.0	35.0	38.0
60-61	36.823500224805606	38.0	38.0	38.0	35.0	38.0
62-63	36.82939412976718	38.0	38.0	38.0	35.0	38.0
64-65	36.78410005072578	38.0	38.0	38.0	35.0	38.0
66-67	36.78700659770328	38.0	38.0	38.0	35.0	38.0
68-69	36.80149399811181	38.0	38.0	38.0	35.0	38.0
70-71	36.71479730833515	38.0	38.0	38.0	34.5	38.0
72-73	36.66763808110656	38.0	38.0	38.0	34.0	38.0
74-75	36.719742248824176	38.0	38.0	38.0	34.5	38.0
76-77	36.69124831974262	38.0	38.0	38.0	34.0	38.0
78-79	36.70937649050384	38.0	38.0	38.0	34.5	38.0
80-81	36.73497571230503	38.0	38.0	38.0	35.0	38.0
82-83	36.603246935230075	38.0	38.0	38.0	34.0	38.0
84-85	36.62030083861579	38.0	38.0	38.0	34.0	38.0
86-87	36.61777013848185	38.0	38.0	38.0	34.0	38.0
88-89	36.5627380659987	38.0	38.0	38.0	34.0	38.0
90-91	36.56235420610996	38.0	38.0	38.0	34.0	38.0
92-93	36.61124414679187	38.0	38.0	38.0	34.0	38.0
94-95	36.50587213438003	38.0	38.0	38.0	34.0	38.0
96-97	36.4629698190928	38.0	38.0	38.0	34.0	38.0
98-99	36.529791406531146	38.0	38.0	38.0	34.0	38.0
100	34.93524055526974	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	39.0
19	51.0
20	123.0
21	296.0
22	988.0
23	2721.0
24	6336.0
25	12579.0
26	21804.0
27	34976.0
28	52240.0
29	74737.0
30	100903.0
31	135681.0
32	180825.0
33	251010.0
34	386982.0
35	730027.0
36	2150839.0
37	4899963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.82859514981984	24.145085638509155	14.702136251969453	24.324182959701552
2	13.700204177138705	21.395373370807093	40.327660959791785	24.57676149226241
3	16.23352008287398	26.49093322575994	32.46577953999988	24.80976715136621
4	12.37209071905208	18.87393388659322	40.12963213613697	28.62434325821773
5	13.095661349659057	37.275061339241866	36.22583324550968	13.40344406558939
6	28.41849157028222	39.55036123570544	19.431908833295036	12.599238360717301
7	24.935796551812604	33.209670370694745	24.97280808145146	16.881724996041193
8	23.401239040296677	37.47348262680789	22.057378818479624	17.067899514415807
9	25.01221093101977	16.771543737028132	22.254448046566193	35.961797285385906
10-11	23.297143422054848	27.278663975562438	30.01760563380282	19.406586968579898
12-13	24.075933681354506	24.941773465995396	30.03778670389272	20.944506148757377
14-15	21.544823870678375	26.261211828473513	27.3239801537698	24.869984147078313
16-17	21.251490634656502	29.226225704203234	28.0824013549294	21.43988230621086
18-19	21.313250238191916	28.596675840483517	29.449400694096113	20.640673227228454
20-21	22.185859115982844	27.763347725050906	29.113578236365754	20.937214922600493
22-23	21.862891791392418	27.657890279970047	28.969148018372394	21.510069910265138
24-25	21.602950548182104	27.815087552927807	29.088285588291807	21.493676310598282
26-27	21.642323233384523	27.949533250864455	28.921856777332376	21.486286738418645
28-29	21.608966044653346	27.937962491152753	29.050869830288256	21.402201633905644
30-31	21.837622790229616	27.796401152433358	28.93227268265242	21.433703374684605
32-33	21.66220264482146	27.943613451881266	28.836772350174968	21.557411553122304
34-35	21.72658248859564	27.959921713706017	28.867421812734474	21.446073984963874
36-37	21.71431745969604	27.869101883891496	28.867363957729225	21.549216698683242
38-39	21.589911750756805	27.995117120027253	28.952416532231013	21.46255459698493
40-41	21.83368128531919	27.85949325094918	28.85536562020721	21.45145984352442
42-43	21.69114962430861	27.961062573000785	28.694679315241533	21.653108487449067
44-45	21.75740226321746	27.998243061279172	28.58976272282539	21.654591952677976
46-47	21.82227186423549	27.965385725341296	28.49113345785024	21.721208952572976
48-49	21.772278942596188	28.063259006958337	28.490163297838794	21.67429875260668
50-51	21.71554373177549	28.112821400326403	28.42001800206929	21.75161686582881
52-53	21.892254879570824	28.1080715392113	28.304257893253894	21.69541568796398
54-55	21.928992077352103	27.93618874232413	28.37209780270135	21.762721377622412
56-57	21.873090860619314	27.992094864428196	28.338223220223963	21.796591054728527
58-59	21.7779309005015	28.067892189733534	28.443396323313415	21.710780586451552
60-61	21.871946752334868	28.065858432213613	28.435225202263776	21.626969613187747
62-63	21.932609066187123	28.085706463350828	28.346779964806153	21.6349045056559
64-65	21.85112056538231	28.090252356377103	28.32231662948619	21.736310448754402
66-67	21.89821165731771	28.172868814583712	28.196174281821335	21.732745246277247
68-69	21.866787710687095	28.058635332892568	28.256313500619612	21.818263455800725
70-71	21.85805996491612	27.92637732557685	28.290658281827486	21.924904427679543
72-73	21.915442220810633	27.93867466685313	28.236578418499512	21.909304693836724
74-75	21.845976535986246	28.001118454625175	28.201046863620377	21.951858145768206
76-77	21.975843215148725	27.958027095050397	28.080997562986337	21.985132126814545
78-79	21.911865558385816	28.029804918438938	28.067850968366898	21.990478554808345
80-81	21.959868169285198	28.02503412272114	28.03328479042639	21.981812917567275
82-83	21.954591798735606	27.992308943274303	28.132290927979316	21.92080833001077
84-85	22.049231004918724	28.034824677746855	28.044196739925596	21.871747577408822
86-87	21.93435178677653	28.03734558524093	28.109761386625703	21.91854124135683
88-89	21.978814060781403	28.013155659273554	28.08232400946415	21.925706270480894
90-91	22.04155608589302	28.08199365412158	28.033796023111368	21.842654236874033
92-93	22.076029067136936	28.041936739325628	28.024205079990562	21.857829113546874
94-95	21.973380628219	28.09461674935631	28.05793426312938	21.874068359295308
96-97	21.996543791059363	28.165695332549284	28.16987612045798	21.66788475593338
98-99	23.045129228105203	30.07491853417149	28.38375741096901	18.496194826754294
100	21.876382254714066	30.992776244552022	22.45143131042372	24.67941019031019
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	788.0
1	947.0
2	1676.5
3	2432.0
4	3023.5
5	3915.5
6	4725.5
7	5720.0
8	6842.0
9	7775.5
10	8297.0
11	8654.0
12	9677.0
13	11793.0
14	15507.5
15	19738.5
16	22627.0
17	23774.5
18	23911.5
19	23563.0
20	23262.5
21	24163.5
22	27918.5
23	35159.0
24	46007.0
25	61599.5
26	81043.5
27	102002.0
28	123987.0
29	146657.5
30	167913.0
31	189755.5
32	212785.5
33	234877.0
34	256891.0
35	277363.5
36	297548.5
37	315987.5
38	327918.5
39	337750.0
40	349275.0
41	358180.5
42	362618.0
43	364917.5
44	365893.0
45	366420.5
46	364066.0
47	359625.0
48	354447.5
49	346347.0
50	335239.0
51	320294.5
52	301356.5
53	279773.0
54	258157.0
55	236798.5
56	215860.0
57	194361.0
58	170845.5
59	147018.0
60	122498.5
61	98523.0
62	76968.0
63	59043.0
64	44868.0
65	34369.5
66	26726.5
67	19302.0
68	13310.5
69	9931.5
70	6925.0
71	4208.0
72	2893.5
73	1904.5
74	1145.5
75	665.5
76	401.5
77	232.0
78	142.5
79	78.0
80	45.0
81	34.5
82	31.0
83	23.5
84	10.5
85	3.0
86	3.0
87	2.5
88	2.0
89	2.5
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0042573764299255745
6	2.2116241194418567E-5
7	0.0
8	0.0
9	1.1058120597209283E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.658853650290869E-5
26-27	0.0
28-29	2.7654123899103156E-5
30-31	0.0
32-33	2.7697590176706197E-5
34-35	0.0
36-37	4.893662928946239E-4
38-39	4.464753616478334E-5
40-41	2.4652880439749114E-4
42-43	9.001148208968407E-5
44-45	7.066145053260362E-4
46-47	0.0
48-49	5.7181286234708936E-5
50-51	1.1499244470890151E-5
52-53	1.1554633803901573E-4
54-55	0.002136393515790241
56-57	0.0010621617933866537
58-59	1.3499301968702984E-4
60-61	3.8375314352863496E-4
62-63	1.0096098825841523E-4
64-65	7.947548094766564E-4
66-67	0.0013836301751453217
68-69	4.120424246151721E-4
70-71	6.470746305356471E-4
72-73	1.7219484904938296E-4
74-75	1.8578945966851447E-4
76-77	0.0
78-79	0.0015841792295118004
80-81	0.0
82-83	8.367550333210545E-4
84-85	8.367905657399403E-5
86-87	6.487707999162307E-6
88-89	9.812959753519385E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	2.0460584116939603E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	188.0
22-23	409.0
24-25	485.0
26-27	1189.0
28-29	2858.0
30-31	9108.0
32-33	12303.0
34-35	18432.0
36-37	30669.0
38-39	34881.0
40-41	35273.0
42-43	40577.0
44-45	49012.0
46-47	51129.0
48-49	48986.0
50-51	43039.0
52-53	41007.0
54-55	44779.0
56-57	47474.0
58-59	49376.0
60-61	50236.0
62-63	51016.0
64-65	54719.0
66-67	59157.0
68-69	60545.0
70-71	61676.0
72-73	56720.0
74-75	57959.0
76-77	60526.0
78-79	63103.0
80-81	63285.0
82-83	60346.0
84-85	60213.0
86-87	63088.0
88-89	66224.0
90-91	67263.0
92-93	68344.0
94-95	84366.0
96-97	290461.0
98-99	650689.0
100-101	6431973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.23480368415139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.98186078478082	76.69586239125434
2	6.971640079637205	11.884527470888774
3	1.673743495044473	4.279835946532231
4	0.6225070587557484	2.122370677801788
5	0.28366494031012407	1.2089062749704973
6	0.15971035865101094	0.8167728639566595
7	0.08699829807141475	0.5190697999880626
8	0.05322763020407411	0.362947728881349
9	0.03925153017099887	0.30110368215848693
>10	0.1250861172536113	1.612118120872647
>50	0.001968399871425204	0.10964579272016829
>100	2.771834149191531E-4	0.04650329347234986
>500	6.412383420662534E-5	0.040335956502644425
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.211624119441857E-5	0.0
2	0.0	0.0	0.0	2.211624119441857E-5	0.0
3	0.0	0.0	0.0	3.317436179162785E-5	0.0
4	0.0	3.317436179162785E-5	0.0	3.317436179162785E-5	0.0
5	0.0	3.317436179162785E-5	0.0	3.317436179162785E-5	0.0
6	0.0	3.317436179162785E-5	0.0	3.317436179162785E-5	0.0
7	0.0	3.317436179162785E-5	0.0	4.423248238883714E-5	1.1058120597209285E-5
8	0.0	5.5290602986046423E-5	0.0	4.423248238883714E-5	1.1058120597209285E-5
9	0.0	1.5481368836092998E-4	0.0	6.63487235832557E-5	2.211624119441857E-5
10-11	0.0	1.5481368836092998E-4	0.0	8.846496477767428E-5	3.317436179162785E-5
12-13	0.0	1.5481368836092998E-4	0.0	1.050521456734882E-4	3.317436179162785E-5
14-15	0.0	2.101042913469764E-4	0.0	1.437555677637207E-4	6.081966328465106E-5
16-17	0.0	2.211624119441857E-4	0.0	1.4928462806232534E-4	7.740684418046499E-5
18-19	0.0	2.488077134372089E-4	0.0	1.5481368836092998E-4	9.399402507627891E-5
20-21	0.0	2.764530149302321E-4	0.0	1.714008692567439E-4	1.1058120597209285E-4
22-23	1.1058120597209285E-5	3.040983164232553E-4	0.0	1.990461707497671E-4	1.1058120597209285E-4
24-25	1.1058120597209285E-5	3.7597610030511565E-4	0.0	2.1563335164558105E-4	1.2163932656930212E-4
26-27	1.1058120597209285E-5	3.8703422090232494E-4	0.0	2.4327865313860425E-4	1.2163932656930212E-4
28-29	1.1058120597209285E-5	3.980923414995342E-4	0.0	4.1467952239534816E-4	1.437555677637207E-4
30-31	1.1058120597209285E-5	4.4785388418697603E-4	0.0	0.0010505214567348821	1.6587180895813927E-4
32-33	1.1058120597209285E-5	4.533829444855807E-4	0.0	0.002559954918253949	1.6587180895813927E-4
34-35	1.1058120597209285E-5	5.197316680688364E-4	0.0	0.004450893540376737	1.7692992955534856E-4
36-37	2.211624119441857E-5	5.971385122493013E-4	0.0	0.008083486156559988	1.8798805015255782E-4
38-39	2.211624119441857E-5	6.247838137423246E-4	0.0	0.019959907677962758	1.8798805015255782E-4
40-41	2.211624119441857E-5	7.187778388186034E-4	0.0	0.033019548103266926	1.8798805015255782E-4
42-43	2.211624119441857E-5	7.243068991172081E-4	0.0	0.040887400908181326	2.101042913469764E-4
44-45	2.211624119441857E-5	7.795975021032545E-4	0.0	0.04952379309460178	2.211624119441857E-4
46-47	2.211624119441857E-5	8.293590447906963E-4	0.0	0.05976914182791618	2.4327865313860425E-4
48-49	2.7645301493023212E-5	8.34888105089301E-4	0.0	0.06961639821973105	2.4327865313860425E-4
50-51	3.317436179162785E-5	9.952308537488357E-4	0.0	0.07975669480737196	2.4327865313860425E-4
52-53	3.317436179162785E-5	9.952308537488357E-4	0.0	0.1111230538813561	2.4327865313860425E-4
54-55	3.317436179162785E-5	0.0010118180346446496	0.0	0.1502135101924909	2.4327865313860425E-4
56-57	3.317436179162785E-5	0.0010394633361376726	0.0	0.2022474966626592	2.543367737358135E-4
58-59	3.317436179162785E-5	0.0010449923964362773	0.0	0.24208437611410566	2.653948943330228E-4
60-61	3.317436179162785E-5	0.0010615795773320912	0.0	0.2610324657574238	2.653948943330228E-4
62-63	3.8703422090232495E-5	0.0010892248788251144	0.0	0.273754833504513	2.653948943330228E-4
64-65	4.423248238883714E-5	0.0012274513862902306	0.0	0.27834948261265346	2.653948943330228E-4
66-67	4.423248238883714E-5	0.0012274513862902306	0.0	0.2828114342736274	2.7092395463162745E-4
68-69	4.423248238883714E-5	0.0012495676274846491	0.0	0.2866983636635465	2.764530149302321E-4
70-71	4.423248238883714E-5	0.0012550966877832538	0.0	0.2904857699680907	2.875111355274414E-4
72-73	4.423248238883714E-5	0.0012606257480818584	0.0	0.29383638050904515	2.9856925612465067E-4
74-75	4.423248238883714E-5	0.001299329170172091	0.0	0.29626363798013255	2.9856925612465067E-4
76-77	4.423248238883714E-5	0.0013656778937553467	0.0	0.29852502364226186	2.9856925612465067E-4
78-79	4.423248238883714E-5	0.00139332319524837	0.0	0.300145038309753	2.9856925612465067E-4
80-81	4.423248238883714E-5	0.0014043813158455792	0.0	0.3007421768220023	3.151564370204646E-4
82-83	4.423248238883714E-5	0.0014209684967413931	0.0	0.3012010888267865	3.372726782148832E-4
84-85	4.423248238883714E-5	0.0014320266173386022	0.0	0.3014167221784321	3.483307988120925E-4
86-87	4.423248238883714E-5	0.0014596719188316254	0.0	0.3014996580829111	3.593889194093017E-4
88	4.423248238883714E-5	0.0015260206424148812	0.0	0.30152177432410554	3.6491797970790636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8910	0.0	49.44083	1
GTATCAA	15780	0.0	34.067238	1
TCAACGC	18580	0.0	29.406082	4
ATCAACG	18835	0.0	28.868055	3
CAACGCA	19325	0.0	28.249718	5
AACGCAG	20045	0.0	27.343206	6
TATCAAC	20405	0.0	26.79756	2
ACGCAGA	23320	0.0	23.409033	7
CGCAGAG	23900	0.0	22.822573	8
GCAGAGT	29675	0.0	18.469908	9
GAGTACT	15660	0.0	18.25703	12-13
AGAGTAC	26460	0.0	18.016933	10-11
GTACATG	23000	0.0	17.033327	1
CAGAGTA	29290	0.0	17.01085	10-11
TACATGG	23335	0.0	16.93937	2
ACATGGG	23000	0.0	16.80418	3
GTACTTT	17650	0.0	15.974777	14-15
CATGGGG	16615	0.0	14.697279	4
AGTACTT	19305	0.0	13.797547	12-13
ATGGGGG	9565	0.0	12.535458	5
>>END_MODULE